OBJECTIVES/SPECIFIC AIMS: Characterize the expression kinetics of HIV-1 Envelope and their relationship to virus production at the cellular level. METHODS/STUDY POPULATION: In vitro and ex vivo laboratory analyses. RESULTS/ANTICIPATED RESULTS: Initial studies addressing the kinetics of cell surface. Envelope (Env) expression reveal that Env expression to peaks on day 2 post infection. Next steps include a series of experiments to compare the kinetics of Env cell surface expression with broadly neutralizing antibody (bNAb)-mediated ADCC and the characterization of virus production kinetics in this same context. To be maximally effective, ADCC elimination of infected cells should occur before peak Env expression. DISCUSSION/SIGNIFICANCE OF IMPACT: Potent bNAbs to HIV-1 recognize vulnerable sites on the HIV-1 Envelope (Env) protein and are of great clinical interest due to their potential use in the prevention and treatment of HIV-1 infection. Their effectiveness depends not only on the neutralization of viral infectivity, but also on the elimination of productively infected cells via antibody-dependent cellular cytotoxicity (ADCC). On a cellular level, ADCC dynamics are determined by the timing and level of Env expression on the surface of HIV-infected cells. This study aims to delineate the expression kinetics of HIV-1 Envelope and their relationship to virus production. We expect that it will provide new insights into the utility of bNAb-mediated ADCC in treating and possibly curing HIV-1 infection; therefore results might have substantial impact on future HIV treatment strategies.

The lesser date moth (LDM) Batrachedra amydraula is a significant pest of date palm fruits. Previously, detection and monitoring of the pest was inaccurate due to high costs of sampling with lifting machines. We report a practical system for detection and monitoring of LDM based on pheromone traps and relevant models. Dose–response experiments with LDM pheromone traps indicated a 1 mg lure is optimal for monitoring. Delta traps with adhesive covering their entire inner surface gave the highest captures while trap colour was unimportant. Sampling pheromone traps throughout the night indicated male flight began at 1:00–2:00 and reached a peak 2 h before sunrise. Monitoring traps exposed all year long in Israel revealed three generations with different abundance. Trapping transects in a date plantation indicated interference from a monitoring trap became minimal at distances >27 m away. Inter-trap distances closer than this may lower efficiency of monitoring and mass trapping in control programs. Our estimate of the circular effective attraction radius (EARc) of a 1 mg delta trap for LDM (3.43 m) shows this bait is among the most attractive compared with baits for other insects. We developed encounter-rate equations with the pheromone trap EARc to model the interplay between population levels, trap density and captures that are useful for detection of invasive LDM and its control by mass trapping. The integrated methodologies are applicable to many pest species.

Late Neogene Sirius Group strata from Tillite Spur and Quartz Hills in the Reedy Glacier area, Antarctica, demonstrate the variability in Sirius Group facies and contrasts Sirius Group strata deposited at high and low paleo-elevation, respectively. The Tillite Spur and Quartz Hills Formations (Pliocene) are formally defined here.The Tillite Spur Formation type section crops out on the edge of the Wisconsin Plateau overlooking Tillite Spur. It comprises 32m of alternating coarse gray conglomerate and muddy olive-brown diamictites. The Quartz Hills Formation type section crops out above the western margin of Reedy Glacier in a pre-existing cirque towards the southern end of the Quartz Hills. It comprises c.100m of alternating massive diamictites and rhythmically interbedded sandstone and laminated mudstones which were deposited close to sea level and subsequently rapidly uplifted (>500 m Myr−1) to their present elevation at c. 1500 m. Three orders of paleoclimatic variability are recorded in the Sirius Group strata from Reedy Valley: (1) recycled marine microfloras in glacial diamictites indicate intervals of marine incursion into the Antarctic cratonic interior co-occurring with reductions in the East Antarctic ice sheet; (2) an advancing and retreating paleo-Reedy Glacier deposited a glacial/interglacial sequence alternating on a 10-100 kyr scale; 3) Centimeter and millimeter stratification in strata of the Quartz Hills Formation record annual kyr scale variability.