This study provides the first focused investigation of rudist bivalves from the Upper Cretaceous of the Gulf Coastal Plain (GCP) in the southern US and previously undescribed specimens from the Flor de Alba Limestone Member of the Pozas Formation in Puerto Rico. Identified rudists from the GCP comprise the Monopleuridae, including Gyropleura, as well as Radiolitidae, including Biradiolites cardenasensi, Durania maxima, Guanacastea jamaicensis, Radiolites acutocostata, and Sauvagesia. Integrating rudist occurrences within well-established GCP biostratigraphy allows for extension of upper ranges of D. maxima and R. acutocostata into the late Campanian, and extension of the lower ranges of B. cardenasensis and G. jamaicensis into the early Campanian. Identified rudists from Puerto Rico comprise the Hippuritidae and include Barrettia monilifera, which supports the age of the Flor de Alba Limestone Member of the Pozas Formation as middle Campanian. Combined taxonomic, biostratigraphic, and paleobiogeographic analyses indicate there is no rudist fauna endemic to the GCP, and the region marks the northeastern range of the Caribbean genera Biradiolites, Durania, Guanacastea, Gyropleura, Radiolites, and Sauvagesia during the Campanian and Maastrichtian. The new occurrences help inform future updates of Late Cretaceous sea surface-current reconstructions for the Caribbean and Western Interior Seaway, USA.

The ability of dairy cows to discriminate between floors with a smooth epoxy resin surface or with surface-applied bauxite aggregates of mean diameters 0.5 mm, 1.2 mm or 2.5 mm (having coefficients of static friction of 0.35, 0.42, 0.49 and 0.74, respectively) was recorded when they were offered the opportunity to walk down paired floor surfaces to receive a food reward. Following training, one half of the cows were rewarded when they selected the floor with the greater friction and the other half were rewarded when they selected the floor with the least friction. The cows were able to distinguish between the different floor surfaces — even between surfaces with 0.5 mm and 1.2 mm aggregates, which humans found difficult to distinguish. Eight similar cows were then offered a choice of walking down passageways of paired floors with an equal reward at the end of each passageway. There were no consistent preferences for floor type, and when the reward was offered only on the side least favoured by each cow in the initial test, the random pattern of selection was still evident. A final choice test offered the cows the opportunity to traverse passageways of either wet concrete or concrete covered with excreta. All cows avoided the passageway with excreta completely, even when the reward was increased in this passageway and removed from the wet concrete passageway. This avoidance was attributed to the cows’ lack of contact with slurry, as they were at pasture for most of the day, in contrast to the cows used in previous work, which were housed in buildings with passageways covered in excreta and showed little avoidance behaviour of such passageways.

Guidelines for the housing of dairy cows do not address the provision of supplementary lighting in passageways, other than for inspection of the animals. Two experiments were conducted to investigate whether lighting passageways to various intensities influenced the locomotion of dairy cows. The first experiment compared the locomotion of dairy cows in a dark or lighted passageway as they walked back to their accommodation from milking. When the passageway was dark, the cows took shorter but more rapid steps - which probably increased their stability. In the second experiment, cows walked down a cubicle passageway to receive a food reward, with the light intensity in the building varying from 0-250 lux. Step length and stepping rate were recorded, as well as the angles of the cows’ leg joints (which were measured from video recordings). Once again, the cows increased their stepping rate in the dark, and this resulted in an increased walking rate, perhaps because they wished to return more rapidly to other members of their group and found the darkness aversive. In addition, the arcs of travel of the metacarpophalangeal joint and of the fore- and hindfeet angles to the floor were reduced in the dark, probably increasing the cows’ stability, and were greatest at 119 lux. The slowest walking rate was observed at 39 lux. Hence, the optimum illumination for dairy cow locomotion may lie approximately between 39 and 119 lux, as measured by our technique.

We conclude that during locomotion in dark passageways cows have to modify their walking behaviour significantly, so that the provision of at least a low level of lighting is desirable at night.

An experiment is described where 40 spring-calving dairy cows were allocated to be housed in a deep straw yard or a cubicle house from November to April, in order to examine the effects on behaviour, milk production and hoof health. Cows in straw yards spent longer lying down and feeding, except during oestrus when they increased their time spent standing proportionately more than cows in cubicles. In the straw yard cows spent longer in associative behaviour during oestrus and had fewer unsuccessful mounting attempts. There were no differences in milk production or composition but cows in the straw yard lost more weight after calving. Cows in the cubicles had a reduction in heel depth, which is a predisposing factor to lameness. It is concluded that a straw yard system for dairy cows allows greater opportunity to display normal behaviour, leads to better hoof health and provides acceptable levels of production.

Both the presence of slurry and dark conditions may deter dairy cows from using passageways in cubicle accommodation, thus restricting movement and normal behaviour. We attempted to train seven dairy cows to recognize the quantity of reward offered in a transparent tube containing molasses in a Y-maze. Only one cow failed to consistently select the aisle containing the larger reward. The cows were then individually offered the choice of traversing either a passageway with a 5cm-depth of cow excreta or a clean, dry passageway to collect their rewards. The quantity of the rewards on the two sides was varied between zero and 400ml of molasses to determine the price that the cows were prepared to pay for entering the aisle with excreta. Only two of the seven cows showed a clear avoidance of the passageway with excreta. There was a tendency (P < 0.1) for the cows to avoid the passageway with excreta only when it contained no reward and the clean passageway contained a reward of 400ml molasses. Otherwise, there was no clear avoidance of the passageway with excreta. We also investigated whether cows preferred to enter a lighted or unlighted passageway. All the cows, except one, showed a strong avoidance of the dark passage, even when it contained the largest reward and the lighted passage contained no reward. We conclude that dairy cows demonstrate only mild avoidance of passageways with excreta but strongly avoid passageways without lighting. Therefore, we suggest that passageways for dairy cows should be lit at all times.

Extensive sheep farming systems make an important contribution to socio-economic well-being and the ‘ecosystem services’ that flow from large areas of the UK and elsewhere. They are therefore subject to much policy intervention. However, the animal welfare implications of such interventions and their economic drivers are rarely considered. Under Defra project AW1024 (a further study to assess the interaction between economics, husbandry and animal welfare in large, extensively managed sheep flocks) we therefore assessed the interaction between profit and animal welfare on extensive sheep farms. A detailed inventory of resources, resource deployment and technical performance was constructed for 20 commercial extensive sheep farms in Great Britain (equal numbers from the Scottish Highlands, Cumbria, Peak District and mid-Wales). Farms were drawn from focus groups in these regions where participative research with farmers added further information. These data were summarised and presented to a panel of 12 experts for welfare assessment. We used two welfare assessment methods one drawn from animal welfare science (‘needs’ based) the other from management science (Service Quality Modelling). The methods gave complementary results. The inventory data were also used to build a linear programme (LP) model of sheep, labour and feed-resource management month-by-month on each farm throughout the farming year. By setting the LP to adjust farm management to maximise gross margin under each farm's circumstances we had an objective way to explore resource allocations, their constraints and welfare implications under alternative policy response scenarios. Regression of indicators of extensification (labour per ewe, in-bye land per ewe, hill area per ewe and lambs weaned per ewe) on overall welfare score explained 0.66 of variation with labour and lambs weaned per ewe both positive coefficients. Neither gross margin nor flock size were correlated with welfare score. Gross margin was also uncorrelated with these indicators of extensification with the exception of labour/ewe, which was negatively correlated with flock size and hence with gross margin. These results suggest animal welfare is best served by reduced extensification while greater profits are found in flock expansion with reduced labour input per ewe and no increase in other inputs or in productivity. Such potential conflicts should be considered as policy adjusts to meet the requirements for sustainable land use in the hills and uplands.

Animals under human management are often separated from conspecifics, which may lead to behaviour indicative of separation distress or grief. For the purposes of this paper, grief is considered as a biological response to separation, indicated by a bi-phasic ‘protest-despair’ behavioural response. It is reasonable to assume that only animals which are able to form complex social bonds can experience grief. Scientific experiments have suggested that some farm and laboratory animals experience distress or grief as a result of maternal separation and social isolation. However, little is known about whether the public believe that animals are capable of grief. Therefore, we surveyed 1,000 members of the public to establish what knowledge they have about grief in animals and to compare this to what we know in science. The survey revealed that 90% of the general public believed that some or all animals can experience grief, with 23% believing that all animals can grieve. They attributed grief more to companion animals and animals with higher level cognitive abilities than to farm animals and animals that may be feared. It is concluded that public belief about grief in animals extends beyond scientific evidence, and that educating people about scientific findings and management practices connected with grief and separation distress may improve the welfare of farm and laboratory animals.

The mining industry is an important source of noise for wildlife, and the eastern blue-tongued (EBT) lizard (Tiliqua scincoides) is an Australian animal that may be impacted. We analysed the behaviour of nine EBT lizards during and after exposure for 5 s to one of five combinations of mining machinery noise frequency and amplitude (frequency < or > 2 kHz, low [60-65 dB (A)] and high [70-75 dB (A)] amplitude, or a control treatment). Following exposure, lizards could leave the test chamber and enter an escape chamber, which led into a small hiding chamber. Chambers were monitored for 15 min after initial exposure. In the test chamber, lizards exposed to high frequency, high amplitude noise spent more time freezing, a typical stress response in reptiles, when compared with animals in all the other treatments. This was especially the case for lizards exposed to high frequency noise. In the hiding chamber, high frequency noise at high amplitudes decreased durations of head positioning to the right and downwards, suggesting a lateralised fear reaction, but decreased standing and freezing behaviours. We hypothesise that lizards have lateralised behaviour reactions to mining noise, with high frequency, high amplitude noise being the most detrimental. Our results demonstrate that acute exposure to mining noise had negative effects on EBT lizards’ behaviour and welfare, which may suggest a threat for lizards experiencing chronic mining noise in the wild, making the study of mining machinery noise in situ a research priority.

A survey of attitudes towards the welfare and rights of animals was conducted in universities in 11 European and Asian countries, to improve understanding of cultural differences that might impact on trade and international relations. Collaborators’ universities were recruited in each country to assist in the design, translation and administration of the survey via the internet in a convenient selection of the country's universities, providing 3,433 student responses from at least 103 universities. Respondents rated the acceptability of 43 major concerns about animals (focused on type of use, animal integrity, killing animals, animal welfare, experimentation on animals, changes in animal genotypes, the environment for animals and societal attitudes towards animals). Students from European countries had more concern for animal welfare than students from Asian countries, which may be partly explained by increased affluence of European students as there was a positive correlation between student expenditure and concern for animal welfare and rights. Southern and central European countries had most concern for animal rights and unnatural practices. Those in communist or former communist countries in Asia and Europe had most concern about killing animals and those in northern European countries the least. Regional similarities between neighbouring countries were evident in responses to animal issues and there were no differences between ethnic groups within a country. Thus, there were national and continental differences in European and Asian students’ attitudes to animals’ welfare and rights, which appear to arise as a result of the socio-political situation in regions rather than religious or other differences.

Horseshoe crabs as a group are renowned for their morphological conservatism punctuated by marked shifts in morphology associated with the occupation of non-marine environments and have been suggested to exhibit a consistent developmental trajectory throughout their evolutionary history. Here, we report a new species of horseshoe crab from the Ordovician (Late Sandbian) of Kingston, Ontario, Canada, from juvenile and adult material. This new species provides critical insight into the ontogeny and morphology of the earliest horseshoe crabs, indicating that at least some Palaeozoic forms had freely articulating tergites anterior to the fused thoracetron and an opisthosoma comprising 13 segments.

Functional coefficient (FC) regressions allow for systematic flexibility in the responsiveness of a dependent variable to movements in the regressors, making them attractive in applications where marginal effects may depend on covariates. Such models are commonly estimated by local kernel regression methods. This paper explores situations where responsiveness to covariates is locally flat or fixed. The paper develops new asymptotics that take account of shape characteristics of the function in the locality of the point of estimation. Both stationary and integrated regressor cases are examined. The limit theory of FC kernel regression is shown to depend intimately on functional shape in ways that affect rates of convergence, optimal bandwidth selection, estimation, and inference. In FC cointegrating regression, flat behavior materially changes the limit distribution by introducing the shape characteristics of the function into the limiting distribution through variance as well as centering. In the boundary case where the number of zero derivatives tends to infinity, near parametric rates of convergence apply in stationary and nonstationary cases. Implications for inference are discussed and a feasible pre-test inference procedure is proposed that takes unknown potential flatness into consideration and provides a practical approach to inference.

OBJECTIVES/GOALS: This study examines faculty mentor experiences in a summer research program for students traditionally underrepresented in translational research. The objectives are to understand mentor perspectives of the program and how their views were impacted by the pandemic-related pivot to a remote format. METHODS/STUDY POPULATION: During the summer research program, students work with a faculty mentor on an ongoing research project. Program staff pair students with diverse faculty members who share their research interests. Our program has traditionally been offered as a residential in-person program but in 2020 we moved swiftly to a fully remote learning format. Students continued to work on research projects remotely and interacted virtually with their faculty mentors. For the past five cohort years, we have collected comparable data about faculty perspectives of their program experience, which allows us to evaluate the impact of the remote format on the faculty experience compared to that of the in-person format. RESULTS/ANTICIPATED RESULTS: For this study, we will examine data from five cohort years (2017-2021). A survey questionnaire was administered to mentors each year at the end of the summer research program. Data were collected on faculty satisfaction with the quality and amount of student work, the amount of time students spent on their projects, and how mentors communicated with students. In 2020 and 2021, three questions were added regarding satisfaction with the remote format. Quantitative data collected from both the in-person and remote cohorts will be compared using independent samples t-tests. Select quotes from open-ended qualitative questions will be used to illustrate mentors attitudes toward the program. DISCUSSION/SIGNIFICANCE: This study addresses a gap in the literature about summer research programs, as previous work has primarily focused on trainees. As many training programs continue to remain remote or adopt hybrid models, these results have implications for the design of similar mentored research programs.

We present the most sensitive and detailed view of the neutral hydrogen ( ${\rm H\small I}$ ) emission associated with the Small Magellanic Cloud (SMC), through the combination of data from the Australian Square Kilometre Array Pathfinder (ASKAP) and Parkes (Murriyang), as part of the Galactic Australian Square Kilometre Array Pathfinder (GASKAP) pilot survey. These GASKAP-HI pilot observations, for the first time, reveal ${\rm H\small I}$ in the SMC on similar physical scales as other important tracers of the interstellar medium, such as molecular gas and dust. The resultant image cube possesses an rms noise level of 1.1 K ( $1.6\,\mathrm{mJy\ beam}^{-1}$ ) $\mathrm{per}\ 0.98\,\mathrm{km\ s}^{-1}$ spectral channel with an angular resolution of $30^{\prime\prime}$ ( ${\sim}10\,\mathrm{pc}$ ). We discuss the calibration scheme and the custom imaging pipeline that utilises a joint deconvolution approach, efficiently distributed across a computing cluster, to accurately recover the emission extending across the entire ${\sim}25\,\mathrm{deg}^2$ field-of-view. We provide an overview of the data products and characterise several aspects including the noise properties as a function of angular resolution and the represented spatial scales by deriving the global transfer function over the full spectral range. A preliminary spatial power spectrum analysis on individual spectral channels reveals that the power law nature of the density distribution extends down to scales of 10 pc. We highlight the scientific potential of these data by comparing the properties of an outflowing high-velocity cloud with previous ASKAP+Parkes ${\rm H\small I}$ test observations.