Varicella is a vaccine-preventable infectious disease. Since 1 December 2018, the varicella vaccine has been included in the local Expanded Programme on Immunization (EPI) in Wuxi, China, and children born after 1 December 2014 are eligible for free vaccination. To evaluate the effect of varicella vaccination in Wuxi city, we selected 382 397 children born from 2012 to 2016 as subjects. Their disease data were obtained from the Chinese Disease Prevention and Control Information System, and their vaccination data were obtained from the Jiangsu Province Vaccination Integrated Service Management Information System. The incidence of breakthrough varicella cases increased in the first 4 years and reached the peak in the fifth year. With the increase of vaccination rate, the incidence of varicella decreased significantly. The vaccine effectiveness (VE) was found to be 88.17%–95.78% for one dose and 98.65%–99.93% for two doses. Although the VE per dose decreased from 99.57% in the first year to 93.04% in the eighth year, it remained high. These findings confirmed the effectiveness of varicella vaccination in children, supported the use of a two-dose varicella vaccination strategy to achieve better protection, and provided important insights into the optimal vaccination strategy for varicella prevention in children.

Numerous studies have indicated that turbulence typically initiates along the boundary layer of the stationary disk within a rotor–stator cavity. To describe the transition process to turbulence on the stationary side of a closed rotor–stator cavity, a comprehensive approach combining global linear stability analysis with direct numerical simulation was adopted in the present study. The proposed model aligns with that of Yim et al. (J. Fluid Mech., vol. 848, 2018, pp. 631–647), who investigated the stability characteristics of the rotating-disk boundary layer in a rotor–stator cavity. In order to achieve a stable inflow for the stationary-disk boundary layer, we rotate the shroud together with the rotating disk. Through careful global stability analysis, the predominant spiral mode exhibiting the highest instability in the boundary layer of the stationary disk was discerned, corroborating observations from simulations. Initially, the spiral mode undergoes linear amplification, reaches a state of linear saturation and enters the nonlinear regime. Following nonlinear saturation in the flow field, a circular wave mode arises due to the influence of mean flow distortion. As the Reynolds number attained a sufficiently high level, the interplay between the downstream-propagating circular mode and spiral mode amplified disturbances in the boundary layer of the stationary disk, ultimately leading to the development of localised turbulence at the mid-radius of the rotor–stator cavity. Notably, the present study is the first to elucidate the coexistence of laminar–transitional–turbulent flow states in the stationary-disk boundary layer through direct numerical simulations.

The social-sexual environment is well known for its influence on the survival of organisms by modulating their reproductive output. However, whether it affects survival indirectly through a variety of cues without physical contact and its influence relative to direct interaction remain largely unknown. In this study, we investigated both the indirect and direct influences of the social-sexual environment on the survival and reproduction of the mite Tyrophagus curvipenis (Acari: Acaridae). The results demonstrated no apparent influence of conspecific cues on the survival of mites, but the survival and reproduction of mated female mites significantly changed, with the females mated with males having a significantly shortened lifespan and increased lifetime fecundity. For males, no significant difference was observed across treatments in their survival and lifespan. These findings indicate that direct interaction with the opposite sex has a much more profound influence on mites than indirect interaction and highlight the urgent need to expand research on how conspecific cues modulate the performance of organisms with more species to clarify their impacts across taxa.

For the launch vehicle attitude control problem, traditional methods can seldom accurately identify the fault types, making the control method lack of pertinence, which largely affects the effect of attitude control. This paper proposes an active fault tolerant control strategy, which mainly includes fault diagnosis and fault tolerant control. In the fault diagnosis part, a small deviation attitude dynamics model of the launch vehicle is established, Kalman filters with different structures are designed to detect and isolate faults through residual changes, and the fault quantity of the actuator is further estimated. In the fault tolerant control part, the following control scheme is adopted according to the above diagnostic information: when the sensor fault is detected, the sensor measurement data is reconstructed; when the actuator fault is identified, the control allocation matrix is reconstructed. Simulation results show that the proposed method can effectively diagnose sensor fault and actuator faults, and significantly improve attitude tracking accuracy and control adjustment time.

We report how artificial nests can be utilised at scale in nest site-limited areas of Mongolia to create a managed population of Saker Falcons (Falco cherrug), an “Endangered” species that is harvested for international trade. The provision of 5,000 artificial nests created an average annual saker population of 602 (SE ± 59) breeding pairs, producing an estimated 1,735 (SE ± 272) fledglings per annum over the period 2013–2015. Our regular monitoring enabled us to identify the effects of climate and vegetation on breeding performance. A warm and dry climate prior to breeding was associated with earlier egg-laying dates, while warmer conditions during the breeding season increased fledging success. Greater vegetation biomass in the previous growth season was positively related to breeding density and earlier clutch initiation, which was associated with larger clutch size and larger fledged brood size. Furthermore, using small mammal remains from saker pellets collected at artificial nest sites, we found that higher breeding density, earlier egg laying, larger clutch size, and increased nest survival were associated with areas with a higher proportion of small mammal prey in the diet. Our results provided evidence of the role of temporal and spatial variation in climate and prey availability on breeding performance, demonstrating the requirement for dynamic modelling of variable demographic parameters to be incorporated within an adaptive management framework for the sustainable management of the Saker Falcon.

With the development of high-power microwave technology, the output power of the pulse generator is required more and more higher. In this paper, it is realized by increasing the output power of the module while the output impedance of the module changes little. The module of the generator is based on pulse forming network (PFN) and linear transformer (LT). Four Blumlein PFNs with arc-type configuration and 24 Ω characteristic impedance were connected symmetrically to the primary coil of the LTD and driven by two identical laser triggered spark switches to ensure four Blumlein PFNs synchronizing operation. On this basis, a two-stage high-power pulse generator based on PFN-LT is developed. The following technical parameters of the generator were achieved on a 12 Ω high-power solid resistor: output voltage amplitude of ∼250 kV and output power of ∼5.2 GW at a repetition rate of 5 Hz.

Background. We aimed to investigate the associations of various genetic variants in the nicotine metabolism pathway with smoking cessation (SC) in the Chinese Han population. Method. A case-control study was conducted where 363 successful smoking quitters were referred to as cases, and 345 failed smoking quitters were referred to as controls. A total of 42 genetic variants in 10 genes were selectedand genotyped. The weighted gene score was applied to analyze the whole gene effect. Logistic regression was used to explore associations of each genetic variant and gene score with smoking cessation. Results. Our study found that the variants CYP2A6∗4, rs11726322, rs12233719, and rs3100 were associated with a higher probability of quitting smoking, while rs3760657 was associated with a lower probability of quitting smoking. Moreover, the gene scores of CYP2D6, FMO3, UGT2B10, UGT1A9, UGT2B7, and UGT2B15 were shown to exert a positive effect, while the gene score of CYP2B6 was detected to exert a negative effect on successful smoking cessation. Conclusion. This study revealed that genetic variants in the nicotine metabolic pathway were associated with smoking cessation in the Chinese Han population.

Increasing evidence shows that maternal hyperglycemia inhibits cardiomyocyte (CM) proliferation and promotes cell apoptosis during fetal heart development, which leads to cardiac dysplasia. Accumulating evidence suggests that the overexpression of miR-21 in CMs has a protective role in cardiac function. Therefore, we investigated whether miR-21 can rescue CM injury caused by high glucose. First, we performed biological function analysis of miR-21-5p overexpression in H9c2 cells treated with high glucose. We found that the proliferation of H9c2 cells treated with high glucose decreased significantly and was rescued after overexpression of miR-21-5p. CCK-8 and EdU incorporation assays were performed to assess cell proliferation. The cell proliferation of the miR-21-5p mimic transfection group was improved compared with that of the NC mimic group (*p < 0.05, miR-21-5p mimics vs. NC mimics) when the proliferation of H9c2 cells was reduced by high glucose (****p < 0.0001, high glucose (HG) vs. normal glucose (NG)). Then, we verified the targeted and negative regulation of miR-21-5p on Rhob using a dual-luciferase activity assay and RT-qPCR, respectively. We further demonstrated that miR-21-5p regulates Rhob to rescue the inhibition of CM proliferation induced by high glucose. The CCK-8 results showed that the cell proliferation of the siRNA-Rhob group was higher than that of the NC mimic group (***p < 0.001) and that of the cotransfection group with Up-Rhob plasmids and miR-21-5p mimics was lower than that of the miR-21-5p mimic group (*p < 0.05). Conclusion: Overexpression of miR-21-5p rescues the inhibition of high glucose-induced CM proliferation through regulation of Rhob.

Fine mapping and discovery of watermelon rind trait candidate genes are of great significance for modern watermelon breeding and development. In this study, we used the high-resolution genetic mapping and genome-wide genetic variation detection technology, combined with genome survey and sequencing technology, to locate and discover the candidate genes for rind traits of star watermelon varieties ‘Su XuanBai’ and ‘SHLX21’. Firstly, we identified a total of eight quantitative trait loci (QTLs) related to watermelon rind traits on chromosome 6. Secondly, a total of 208,240 single nucleotide polymorphisms and 75,345 small Indels (insertions/deletions) were detected in the two parents by high-coverage re-sequencing, respectively. Based on the genetic variation of the two parents and combined with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis using the planta database, the QTL region was reduced to 0.02 Mb. Finally, we identified the six potential regulatory factors for watermelon rind traits using real-time quantitative PCR. In conclusion, our results revealed the fine localization of candidate genes for watermelon rind traits and the successful discovery of candidate genes for regulating watermelon rind traits, which is of importance for watermelon rind traits and breeding-improved watermelon varieties.

Fast neutron absorption spectroscopy is widely used in the study of nuclear structure and element analysis. However, due to the traditional neutron source pulse duration being of the order of nanoseconds, it is difficult to obtain a high-resolution absorption spectrum. Thus, we present a method of ultrahigh energy-resolution absorption spectroscopy via a high repetition rate, picosecond duration pulsed neutron source driven by a terawatt laser. The technology of single neutron count is used, which results in easily distinguishing the width of approximately 20 keV at 2 MeV and an asymmetric shape of the neutron absorption peak. The absorption spectroscopy based on a laser neutron source has one order of magnitude higher energy-resolution power than the state-of-the-art traditional neutron sources, which could be of benefit for precisely measuring nuclear structure data.

All-fiber coherent beam combiners based on the self-imaging effect can achieve a near-perfect single laser beam, which can provide a promising way to overcome the power limitation of a single-fiber laser. One of the key points is combining efficiency, which is determined by various mismatches during fabrication. A theoretical model has been built, and the mismatch error is analyzed numerically for the first time. The mismatch errors have been numerically studied with the beam quality and combining efficiency being chosen as the evaluation criteria. The tolerance of each mismatch error for causing 1% loss is calculated to guide the design of the beam combiners. The simulation results are consistent with the experimental results, which show that the mismatch error of the square-core fiber is the main cause of the efficiency loss. The results can provide useful guidance for the fabrication of all-fiber coherent beam combiners.

Many rural youths in China receive a poor-quality, strict and exam-oriented education. In everyday and professional discourses, incorporating live-streaming technologies in rural schooling is tied to promises of improved educational quality and a narrowed urban‒rural education gap. Reflecting a dystopian ideology of meritocracy, this article investigates how live-streaming technologies transmit suzhi (human quality) education and downplay the exam-oriented education with which rural students and teachers are familiar. The authors argue that the two educational vehicles for meritocracy work together to channel students to a seemingly meritocratic pathway of social mobility but funnel rural students to an inferior educational track according to their rural registration and lower-class backgrounds. The online version of suzhi education complicates and even exacerbates the already fierce educational competition that rural students face. Rural students’ low aspirations and their teachers’ apathy towards live-streaming classes challenge the purportedly transformative effects of live-streaming technologies in China's rural schooling.

Sterol regulatory element-binding protein 2 (SREBP2) is considered to be a major regulator to control cholesterol homoeostasis in mammals. However, the role of SREBP2 in teleost remains poorly understand. Here, we explored the molecular characterisation of SREBP2 and identified SREBP2 as a key modulator for 3-hydroxy-3-methylglutaryl-coenzyme A reductase and 7-dehydrocholesterol reductase, which were rate-limiting enzymes of cholesterol biosynthesis. Moreover, dietary palm oil in vivo or palmitic acid (PA) treatment in vitro elevated cholesterol content through triggering SREBP2-mediated cholesterol biosynthesis in large yellow croaker. Furthermore, our results also found that PA-induced activation of SREBP2 was dependent on the stimulating of endoplasmic reticulum stress (ERS) in croaker myocytes and inhibition of ERS by 4-Phenylbutyric acid alleviated PA-induced SREBP2 activation and cholesterol biosynthesis. In summary, our findings reveal a novel insight for understanding the role of SREBP2 in the regulation of cholesterol metabolism in fish and may deepen the link between dietary fatty acid and cholesterol biosynthesis.