Microscopy Today: Volume 30 -

Industry News – March 2022
Published online by Cambridge University Press:

25 March 2022, pp. 48-51
- Article
- - You have access
- PDF
- HTML
- Export citation

Electron Optics Instruments LLC
Published online by Cambridge University Press:

25 March 2022, p. 13
- Article
- - You have access
- PDF
- HTML
- Export citation

Microscopy Society of America Focused Interest Groups: A Historical (and Personal) Perspective
Beverly E. Maleeff
Published online by Cambridge University Press:

31 May 2022, pp. 46-49
- Article
- - You have access
- PDF
- HTML
- Export citation
“A Focused Interest Group (FIG) is a group of not less than ten MSA members who have organized, with the approval of MSA Council, to promote a specific discipline relevant to microscopy or microanalysis.” – mission statement from the FIG guidelines.

Walter McCrone (1916–2002): A Life Lived Fully
Cameron Varano
Published online by Cambridge University Press:

31 May 2022, pp. 50-51
- Article
- - You have access
- PDF
- HTML
- Export citation

Highlights from Microscopy and Microanalysis
Published online by Cambridge University Press:

24 November 2022, pp. 52-53
- Article
- - You have access
- PDF
- HTML
- Export citation

MSA Focused Interest Groups: Atom Probe Field Ion Microscopy and Focused Ion Beam Microscopy
Katherine Jungjohann, Daniel Perea, Josh Sugar, David Diercks, KD Derr, Arun Devaraj
Published online by Cambridge University Press:

11 July 2022, pp. 42-45
- Article
- - You have access
- PDF
- HTML
- Export citation

Gatan
Published online by Cambridge University Press:

25 March 2022, p. 14
- Article
- - You have access
- PDF
- HTML
- Export citation

Highlights from Microscopy and Microanalysis
Published online by Cambridge University Press:

25 March 2022, pp. 52-53
- Article
- - You have access
- PDF
- HTML
- Export citation

Dr. John Spence: Beyond the Boundaries
Cameron Varano Casasanta
Published online by Cambridge University Press:

22 September 2022, pp. 44-45
- Article
- - You have access
- PDF
- HTML
- Export citation

Recent Advances in Gas Injection System-Free Cryo-FIB Lift-Out Transfer for Cryo-Electron Tomography of Multicellular Organisms and Tissues
Sven Klumpe, Jakub Kuba, Oda H. Schioetz, Philipp S. Erdmann, Alexander Rigort, Jürgen M. Plitzko
Published online by Cambridge University Press:

31 January 2022, pp. 42-47
- Article
- - You have access
  - Open access
- PDF
- HTML
- Export citation
Cryo-electron tomography (cryo-ET) enables visualization of protein complexes within their native cellular environment at molecular resolution. Most cells and all tissues, however, are too thick to be imaged directly by transmission electron microscopy (TEM). Overcoming this limitation requires the production of thin biological sections called lamellae. The procedure to obtain lamellae of cells, either seeded or grown directly on electron microscopy grids, requires cryo-focused ion beam (cryo-FIB) milling to thin the samples. This method faces an additional challenge when dealing with tissues and multicellular organisms, as these samples must be high-pressure frozen, which embeds the sample in a thick layer of ice. Nonetheless, lamellae can still be prepared from such samples by extracting a small volume and transferring it to a receiver grid for lamella preparation, a process called lift-out. Here, we describe the available workflows to produce lamellae by lift-out at cryogenic conditions and recent developments in gas injection system (GIS)-free approaches to the lift-out transfer. These advances expand the applications of cryo-ET, enabling the investigation of tissues and whole organisms in situ at molecular resolution.