Research was conducted in 2006 and 2007 to evaluate nicosulfuron tank-mixes for field sandbur control and crop injury to ‘Tifton 85’ and ‘Jiggs’ bermudagrass. In 2006, sequential applications of nicosulfuron + metsulfuron controlled field sandbur 86 to 90% 30 d after treatment (DAT) and 77 to 85% 90 DAT. Single applications of nicosulfuron provided less than 63% control 90 DAT. In 2007, sequential applications of nicosulfuron + metsulfuron controlled field sandbur 84 to 95% 42 DAT. Injury data showed Jiggs bermudagrass was generally more sensitive to herbicide treatment than Tifton 85. Injury to Tifton 85 bermudagrass ranged from 0 to 15%, whereas injury to Jiggs was 7 to 29% 22 DAT. Forage yield data for Jiggs showed significant reductions at the first and second harvests but no differences by the third harvest. No yield reduction was noted for Tifton 85 bermudagrass from any herbicide treatment. Results of these studies indicate that nicosulfuron + metsulfuron combinations are a viable option for field sandbur control in bermudagrass pastures.

Tropical signalgrass (TSG) causes serious problems for sod production and turf maintenance in Florida. Other grasses such as large crabgrass (CG), smutgrass (SG), thin paspalum (TP), and torpedograss (TG) can be problematic as well. Several emulsion formulations composed of mycelium or mycelium-free culture filtrate (or both) of the fungal pathogen Drechslera gigantea (DG) and Sunspray 6E oil were tested with or without ammonium sulfate or pelargonic acid (n-nonanoic acid; a natural product registered as a biorational herbicide) in greenhouse and field trials. A 30% Sunspray 6E oil formulation containing DG mycelium (10 g), DG culture filtrate (70 ml), and 4.5 g of ammonium sulfate caused 88 to 100% injury on TSG, CG, SG, and TG in greenhouse trials. The injury resulted from disease as well as phytotoxicity of the culture filtrate, oil, and ammonium sulfate. An emulsion formulation composed of 30% Sunspray 6E oil and 70% DG culture filtrate amended with 2% (v/v) pelargonic acid killed SG 2 wk after application. DG formulations containing ammonium sulfate or pelargonic acid produced lower levels of injury when treated grasses were exposed to a 24-h dew period compared with those treated and not exposed to dew. Formulations containing DG mycelium, DG culture filtrate, and ammonium sulfate or pelargonic acid are effective and promising for control of weedy grasses. Further evaluations of these formulations under field conditions are justified.

Annual grass weed control and switchgrass cultivar response to PRE-applied pendimethalin and POST-applied mesotrione and quinclorac was evaluated in 2005 and 2006 near Paterson, WA, in both newly seeded and 1-yr-old established switchgrass. Pendimethalin applied to newly planted switchgrass at 1.1 kg ai ha−1 at the one-leaf stage in 2005 or at 0.67 kg ha−1 PRE in 2006 severely injured and greatly reduced switchgrass stands. Mesotrione applied POST at 0.07 kg ai ha−1 injured newly planted switchgrass, reduced switchgrass height for several weeks after treatment, and reduced final switchgrass biomass by 54% both years. ‘Kanlow’ and ‘Cave-in-Rock’ cultivars were injured less by mesotrione than ‘Shawnee’ in 2005, whereas in 2006, Kanlow was injured less than Shawnee and Cave-in-Rock. Quinclorac applied POST at 0.56 kg ai ha−1 injured newly planted switchgrass less than mesotrione and pendimethalin but reduced final switchgrass biomass by 33% both years compared with treatment with atrazine alone. All three herbicide treatments controlled large crabgrass in the year of establishment. Green foxtail counts were reduced 93% or more by pendimethalin and quinclorac compared with nontreated controls, but mesotrione failed to control green foxtail. Pendimethalin applied PRE at 1.1 kg ha−1 did not injure 1-yr-old established switchgrass or reduce switchgrass biomass. Quinclorac applied POST at 0.56 kg ha−1 to established switchgrass reduced switchgrass biomass of the first harvest by 16% in 1 of 2 yr. Mesotrione applied POST at 0.07 kg ha−1 injured established switchgrass and reduced biomass of the first harvest by 33 and 17% in 2005 and 2006, respectively. Kanlow was injured the least by mesotrione in both years. Established switchgrass suppressed late-emerging annual grass weeds sufficiently to avoid the need for a grass-specific herbicide application.

Field experiments were conducted from 2007 through 2009 at four locations in Missouri to evaluate the effect of May and August herbicide applications on weed control, total biomass yield, and forage nutritive values. Experiments were conducted in established tall fescue pastures that contained natural infestations of common ragweed and tall ironweed. Treatments consisted of 2,4-D, metsulfuron, aminopyralid, 2,4-D + dicamba, 2,4-D + picloram, aminopyralid + 2,4-D, and 2,4-D + dicamba + metsulfuron. All herbicide treatments provided > 76% control of common ragweed 1 mo after treatment (MAT), except metsulfuron alone which provided ≤ 62% control. August applications provided greater reductions in common ragweed density than May applications the following spring. Few differences in tall ironweed density were observed, but metsulfuron-containing herbicides tended to provide the lowest reduction in tall ironweed stem density the following spring. Biomass yields were generally greater in nontreated compared to herbicide-treated plots. Crude protein (CP) concentration and relative feed value (RFV) were higher in nontreated compared with herbicide-treated biomass. Overall, the poorer nutritive values and lower biomass yields in the herbicide-treated compared with the nontreated biomass may be partially explained by the removal of common ragweed, tall ironweed, and legumes with the herbicide treatments. Pure samples of common ragweed and white clover were greater in nutritive values than pure samples of tall fescue at all June harvests. Results indicate that common ragweed offers nutritive values equivalent to or greater than tall fescue and white clover when harvested in June at the vegetative stage of growth and that the removal of common ragweed and tall ironweed with herbicide applications is not likely to improve forage nutritive values of the total harvested biomass of tall fescue pastures, at least by the season after treatment.

A field survey was conducted in 2007 to characterize weed populations in different turfgrass sites throughout the Klang Valley of western Peninsular Malaysia. Sites included golf course putting greens, athletic fields, sod farms, and residential lawns. Weeds present in each site were identified and the data were used to calculate frequency, distribution uniformity, density, relative abundance, and community coefficient values for each species. Seventy-nine weed species, belonging to 16 families, were found. The most species were found on residential lawns, and the fewest were found on golf course putting greens; athletic fields and sod farms ranked intermediately. A total of 19 different weed species were classified as major (relative abundance ≥ 15), and abundance rankings varied by turfgrass area. Greater kyllinga had the highest relative abundance values on athletic fields (45.5) and golf course putting greens (71.5), and the second highest value (21.7) on residential lawns. Forked fringerush and annual sedge had the greatest relative abundance values on residential lawns and sod farms, respectively. Cogongrass was reported on 15% of the residential lawns evaluated. The heterogeneity of weed species composition suggests that control strategies will vary by turfgrass use area. Quantifying weed population dynamics will help researchers delineate integrated weed management strategies to turfgrass managers in Malaysia.

Dr. Raghavan Charudattan has worked in the area of biological control of weeds with plant pathogenic fungi for nearly four decades. He has maintained his research program in this line throughout his career. The scientific discoveries and contributions that he has made have been recognized by his peers and demonstrated through his election as fellow of both the Weed Science Society of America and the American Phytopathological Society. The basic knowledge that he has contributed to our understanding of the fundamental biology of weed/pathogen interactions and his contributions in the areas of mycology, etiology, and natural products will have long-lasting effects. Equally important to the basic and applied research that he has conducted is his role as a mentor and colleague. Dr. Charudattan has contributed to the scientific development of more than 60 students, postdocs, and scientists from more than 20 countries. Dr. Charudattan has contributed to the establishment of biological control of weeds with pathogens as a permanent and highly productive area of weed control research.

Several sulfonylurea herbicides are labeled for use on established bermudagrass or seashore paspalum, but label recommendations for many of these chemicals vary for sprigged turf. The objective of this study was to determine the safety of various sulfonylurea herbicides on newly planted, ‘Tifway’ bermudagrass and ‘Aloha’ seashore paspalum sprigs in Arkansas and Louisiana. Treatments were arranged as a five by two by two factorial with five herbicides (foramsulfuron at 29 and 59 g ai ha−1, halosulfuron at 35 and 70 g ai ha−1, metsulfuron at 21 and 42 g ai ha−1, sulfosulfuron at 66 and 131 g ai ha−1, and trifloxysulfuron at 28 and 56 g ai ha−1), two herbicide rates (low and high), and two application timings at 2 or 4 wk after sprigging (WAS). There was no discernable herbicide injury to, or reduction in, Tifway bermudagrass coverage in Arkansas, regardless of herbicide, application timing, or application rate. Trifloxysulfuron and metsulfuron were more injurious than other herbicides in Louisiana when applied at 2 WAS to Tifway bermudagrass, but injury levels were acceptable (< 15%), and there was no long-term reduction in establishment. Metsulfuron or halosulfuron applied at 2 or 4 WAS and sulfosulfuron applied at 4 WAS allowed > 90% establishment of Aloha seashore paspalum at both locations. Both trifloxysulfuron and foramsulfuron were injurious to seashore paspalum and reduced its establishment. These results suggest that sulfonylurea herbicides can be safely applied shortly after sprigging to Tifway bermudagrass and that metsulfuron, halosulfuron, and sulfosulfuron could be useful herbicides for establishing Aloha seashore paspalum from sprigs.

Planting glyphosate-resistant sugarbeet in narrow rows could improve weed control with fewer herbicide applications and cultivations. Field studies were conducted in 2007 and 2008 at multiple locations in Michigan to compare weed management and sugarbeet yield and quality in glyphosate-resistant sugarbeet planted in 38-, 51-, and 76-cm rows. At all locations, weed densities and biomass were less after glyphosate treatments than after conventional herbicide treatments. Weed densities and biomass also were less in 38- and 51-cm rows compared with 76-cm rows following a single glyphosate application when weeds were 10 cm tall. Averaged over row width, sugarbeet treated with glyphosate when weeds first reached 2 cm in height and again as needed thereafter yielded similarly to sugarbeet treated when weeds were 5 to 10 cm tall. However, root yields were reduced when glyphosate application was delayed until weeds averaged 15 cm in height. Sugarbeet root and sugar yields were greater from 38- and 51-cm row widths than from the 76-cm row widths, averaged over all herbicide treatments. Regardless of row width, initial glyphosate applications should be made before weeds reach 10 cm in height to maximize yield and minimize weed competition with sugarbeet.

Proso millet is an important short-season summer cereal in western Nebraska, southeast Wyoming, and eastern Colorado. The objective of this study was to evaluate proso millet tolerance to saflufenacil applied early preplant (EPP) or PRE. Field studies were conducted in Lingle, WY and Sidney, NE in 2008 and 2009. A dose–response study was conducted in the greenhouse at the University of Wyoming in Laramie, WY to determine proso millet cultivar response to saflufenacil applied at six rates from 0 to 400 g ai ha−1. In the field, saflufenacil was applied EPP and PRE at 50 and 100 g ha−1. Proso millet stands were reduced by an average of 33 and 23% by PRE and EPP treatments compared with the nontreated check; however, proso seed yields were not affected by saflufenacil timing or rate. In the greenhouse, ‘Panhandle’ and ‘Dawn’ exhibited less tolerance to saflufenacil than ‘Sunrise’, the cultivar used in the field studies.

Nursery container preemergence herbicides must be applied multiple times, usually every 6 to 8 wk, in order to maintain acceptable weed control. Nursery growers have identified extended duration of container preemergence activity as a research priority for reduction of herbicide usage and costs. The objective of this study was to determine if the combination of slow-release (microencapsulated [ME]) formulations of alachlor and acetochlor with wood-based organic mulches could provide extended efficacy and reduced phytotoxicity vs. over-the-top (OTT) sprays or mulch alone. Efficacy and phytotoxicity studies were conducted over 3 yr with various plants. Both acetochlor formulation OTT sprays reduced spirea shoot dry weights at 45 and 110 days after treatment (DAT) compared with the controls, and emulsifiable concentrate (EC) acetochlor OTT spray also reduced shoot dry weights of rose. No herbicide-treated bark mulch (TBM) combination reduced rose or spirea shoot dry weights. EC acetochlor + hardwood (in 2003) was the only treatment to provide 100% weed control at 45 and 110 DAT. The addition of EC or ME acetochlor to mulch reduced phytotoxicity and extended efficacy in 2002 and 2003; alachlor EC or ME TBM did not. Regardless of bark type, 3-yr average EC and ME TBM were 80% more effective than untreated bark mulch (UBM) and 83% and 98% more effective at 45 and 110 DAT, respectively than their comparable OTT sprays. Of the eight treatments that received ratings above commercially acceptable, averaged over dates and years, the three providing the least phytotoxicity and greatest extent, consistency, and duration of efficacy were all TBM combinations: EC acetochlor + Douglas fir or hardwood bark, EC acetochlor + pine, and ME acetochlor + pine. TBM-reduced phytotoxicity compared with OTT sprays.

A complex coacervate formulation was developed for Colletotrichum truncatum 00-003B1 (Ct), a bioherbicidal fungus against scentless chamomile, and tested in the greenhouse. A two-step process was developed to formulate Ct conidia: (1) invert emulsion preparation—emulsify an aqueous suspension of Ct conidia in nonrefined vegetable oil with the aid of a surfactant, and (2) encapsulate the Ct conidia invert emulsion by complex coacervation. Formulation ingredients, including nonrefined vegetable oils, surfactants, proteins, and carbohydrates, and formulation-processing parameters, including mixing speed and the amount of oil added to invert emulsions, were examined for maximum retention of Ct conidia in the formulation. Most formulation ingredients considered and tested in this study were compatible with Ct, with no significant reduction in conidial germination and mycelial growth. The surfactant soya lecithin promoted the greatest retention of Ct conidia (88%) in the invert emulsion, followed by sorbitan monooleate (82%), glycerol monooleate (70%), and sorbitan trioleate (55%). Optimal retention of Ct conidia in the invert emulsion was observed with a water : oil ratio of 1 : 1.8 to 1 : 3.7, and an overhead paddle stirring speed of 300 rpm when preparing the emulsion. Complex coacervate wall ingredients of 1% gelatin and 2% gum arabic were most effective for Ct conidia retention. In greenhouse studies, scentless chamomile disease rating, following a 24-h dew period, was higher on plants sprayed with the Ct conidia complex coacervate formulation than on plants with Ct conidia suspended in 0.1% Tween 80.

Broad- host-range pathogens are appealing as candidates for commercial development as bioherbicides because of the wider market potential of a product that is effective against a range of weeds. But when these pathogens are able to spread in space (or time), a risk analysis is necessary. Here we test the hypothesis that a safety zone around a bioherbicide application site is adequate so long as it is wide enough to ensure that dispersing inoculum has diluted sufficiently that the density of inoculum occurring naturally in a susceptible crop is no more than doubled by the influx of bioherbicide spores. To this end the plant disease–pathogen inoculum density relationship using data from nine published experiments was modeled using the logistic equation. This revealed that a doubling of the natural spore density of a plant pathogen in the range of 103.4 to 106.7 spores/ml may generally be expected to result in unacceptable increases in disease in a susceptible crop. A doubling outside this range (< 103.4 or > 106.7) is less likely to do so. Therefore when the natural density of inoculum in a crop's environment occurs outside this range, an “acceptable” safety zone for the pathogen's use as a bioherbicide can in most cases be defined by the 1 : 1 ratio of added : natural inoculum. However, if a more “risk averse” safety zone is desired, it can be defined using a 1 : 10 ratio of added : natural inoculum.

The use of triclopyr for the removal of woody and broad-leaf vegetation in right-of-ways and agricultural settings has been proposed for Alaska. Triclopyr concentrations in soil after application are of concern because residual herbicide may affect growth of subsequent vegetation. In order to measure triclopyr residues in soil and determine the amount of herbicide taken up by the plant, soil bioassays were developed. Four agricultural species, turnip, lettuce, mustard, and radish, were tested to determine sensitivity to triclopyr in a 1-wk bioassay. The sensitivity (I50) of turnip, lettuce, mustard, and radish was 0.33 ± 0.05 kg ai ha−1, 0.78 ± 0.11 kg ai ha−1, 0.78 ± 0.07 kg ai ha−1, and 0.85 ± 0.10 kg ai ha−1 (mean ± SE), respectively. Mustard was the most consistent crop in the bioassay with a midrange response to triclopyr and lowest standard deviation for germination as compared to the other species. Thus, it was used in a bioassay to determine triclopyr concentrations in a field trial. The bioassay of mustard closely matched residual amounts of triclopyr in a field trial determined by chemical extraction. Estimates of residual triclopyr concentrations using the bioassay method were sometimes less than the triclopyr concentration determined using a chemical extraction. These differences in concentrations were most evident after spring thaw when the chemical extraction determined there was enough triclopyr in the soil to reduce mustard growth over 60%, yet the bioassay measured only a 10% reduction. The chemical extraction method may have identified nonphototoxic metabolites of triclopyr to be the herbicidal triclopyr acid. These methods, when analyzed together with a dose–response curve, offer a more complete picture of triclopyr residues and the potential for carryover injury to other plant species.

Glyphosate plus flumioxazin tank mixtures have become popular in the nursery production and landscape maintenance industries in the southeastern United States. Research was conducted to compare the efficacy of such a mixture relative to the components applied alone. Glyphosate, flumioxazin, and glyphosate plus flumioxazin (2 : 1, w/w) were applied POST in container trials to four weed species at a series of rates that ranged from no effect to death. Regression analyses revealed that control data from all three treatment series could be described by the four-parameter, log-logistic model. With respect to glyphosate and flumioxazin applied alone, analysis revealed that across all four species, a lower rate of flumioxazin was required for 90% control than of glyphosate. The rate of the mixture required for 90% control was generally intermediate to the components applied alone and ranged from 0.36 kg ha−1 for hairy bittercress to 1.52 kg ha−1 for eclipta. Glyphosate alone was more cost effective than either flumioxazin alone or the mixture for the POST-applied control of all four species. The popularity of the tank mixture might be the result of flumioxazin-based PRE activity that was not measured in this study.

Tenuazonic acid (TeA), a naturally occurring product of Alternaria alternata, a pathogen to croftonweed, was discovered to be a novel natural photosystem II (PSII) inhibitor. However, herbicidal activity of AAC-toxin, a metabolite of this fungus containing TeA as the main active ingredient, has not been evaluated systematically. In this study, we conducted activity-evaluation experiments in the laboratory, greenhouse, and field trials to assess the herbicidal potential of this fungal metabolite. AAC-toxin had high herbicidal activity on all species tested: croftonweed, large crabgrass, barnyardgrass, redroot pigweed, and eclipta. The AAC-toxin caused brown, leaf spot symptoms and leaf necrosis, subsequently killing the seedlings. When AAC-toxin was applied POST at 83 ml ai/ha, more than 95% of large crabgrass, barnyardgrass, and redroot pigweed plants were controlled 2 d after treatment in field trials. It can be concluded that AAC-toxin has broad-spectrum, rapid, and high herbicidal activity similar to that of paraquat and may have the potential to be developed as a microbe-based herbicide.

There is significant interest from processors in producing organic sweet corn and snap bean. However, large-scale production is necessary for this to be a practical and economical venture for processors. This study focused on the feasibility of managing weeds in organic sweet corn and snap bean, utilizing methods that are practical in large hectarage. Tactics such as rotary hoe, interrow cultivation, and a stale seedbed were evaluated alone or in combination. Hand-weeded and herbicide-based treatments were included for each crop for comparison. Percentage weed control, weed biomass, and crop yield were quantified, and net profit was calculated for each treatment. Organic weed management was feasible in snap bean, with yields similar among several of the organic treatments and the herbicide treatment in all 3 yr of the study. Interrow cultivation was the most effective means of organic weed control in snap bean. Organic weed management was possible in snap bean because it is a short-season crop and an effective competitor with weeds in the crop row. Organic weed management was more difficult in sweet corn because of the longer crop season and poor competition with weeds in the crop row. In sweet corn, the organic treatment involving three interrow cultivations was the only one consistently similar in yield to the herbicide treatments. Higher net profits were attained for most of the organic treatments in both crops because of the organic premium. Market saturation and organic premium adjustments are factors for grower consideration in this potential industry, particularly for sweet corn production.

The effect of soil properties and weather on herbicide persistence and injury to following crops were studied at a site near Lethbridge, Alberta, Canada, with undulating topography that included no-tillage and conventional tillage systems on adjacent fields. Soil pH ranged from 5.2 (lower slope no-tillage) to 7.8 (upper slope conventional tillage) and soil organic matter content ranged from 2.3% (upper slope conventional tillage) to 4.4% (lower slope no-tillage). During the years when the experiments were conducted rainfall ranged from < 50% of normal to > 150% of normal. During dry years atrazine and metsulfuron severely injured wheat and lentil crops, seeded 1 yr after herbicide application, on upper slope locations. The most severe injury occurred on the upper slope conventional tillage location. In years with high rainfall, no crop injury occurred 1 yr after atrazine and metsulfuron application on either upper or lower slope locations in both tillage systems. Imazamox plus imazethapyr caused almost 100% injury in the lower slope position in the no-tillage system (pH 5.2) in the driest year. Following-crop injury due to the imidazolinone herbicides decreased with increasing rainfall and increasing soil pH. The most severe injury to following crops seemed to occur when herbicide dissipation was dependent on microbial activity and rainfall was below normal.

Pathogens were not seriously considered as biological control agents for aquatic plants in the United States until the Chesapeake Bay Eurasian watermilfoil decline occurred in the 1960s. The decline and suggestion that it was induced by pathogens spawned interest in the use of pathogens as biological control agents for nuisance aquatic species. In the years that followed, emphasis was placed on finding pathogen agents for some of the most problematic aquatic weeds, including waterhyacinth, Eurasian watermilfoil, and hydrilla. The scientist that has contributed the most to our knowledge of pathogen biological control in aquatic plants has been Dr. Raghavan Charudattan (University of Florida, Gainesville, FL). For the past 40 yr, he has authored or coauthored more than 50 manuscripts devoted to the subject in peer-reviewed journals, books, and proceedings.

In greenhouse and field experiments conducted over 3 yr, tank mixtures of spores of the fungi Colletotrichum truncatum and Colletotrichum gloeosporioides, formulated in unrefined corn oil and Silwet L-77 surfactant, were evaluated as a mycoherbicide mixture for simultaneous control of hemp sesbania and sicklepod, respectively. In greenhouse tests, 100% mortality and dry weight reduction of hemp sesbania occurred 6 d after treatment (DAT), whereas 15 d were required to achieve 100% mortality and dry weight reduction of sicklepod. In field experiments conducted in narrow-row (51-cm) soybean test plots, a single application of the fungal mixture formulated as described controlled hemp sesbania and sicklepod 94% and 88%, respectively, 28 DAT. Neither fungus, applied as aqueous conidial suspensions, provided control of their respective weed hosts. These results indicate that tank mixtures of these anthracnose-forming pathogens can effectively control hemp sesbania and sicklepod with a single application.

Three years of field trials have been carried out in Zaragoza, Spain, using different biodegradable mulch materials in processing tomatoes. The aim was to evaluate weed control with several biodegradable mulches as alternatives to black polyethylene (PE) mulch. The treatments were rice straw, barley straw, maize harvest residue, absinth wormwood plants, black biodegradable plastic, brown kraft paper, PE, herbicide, manual weeding, and unweeded control. Assessments focused on weeds and on crop yield. A laboratory study showed that 1 kg/m2 of organic mulch was sufficient to cover the soil for rice, barley straw, and maize harvest residue. The most abundant weed species in the field were purple nutsedge, common purslane, common lambsquarters, and large crabgrass and a change in weed composition was observed between treatments and years. Most weed species were controlled by the mulching materials except that purple nutsedge was controlled only by paper mulch. The other species were well controlled by PE and biodegradable plastic and also by some of the organic mulch treatments. Best weed control and lowest weed biomass were achieved by paper followed by PE and biodegradable plastic. The best organic mulch was rice straw and the worst weed control was from absinth wormwood. Tomato yield was highest for PE followed by paper, manual weeding, biodegradable plastic, and rice straw and was clearly related to weed control. Paper, biodegradable plastic, and rice straw are potential substitutes for PE and herbicides.