Burcucumber (Sicyos angulatus L.) is a highly invasive vine that has become naturalized in the humid Black Sea region of Turkey, but previous to this study there was no information on the germination biology of this weed. The germination biology of three naturalized populations of S. angulatus from the Black Sea region was studied in laboratory and greenhouse experiments. The germination of nondormant seeds was observed under different photoperiods, constant temperatures, and varying levels of pH, salinity, and osmotic potential. Furthermore, seedling emergence from various soil depths was investigated. The seeds of all populations proved nonphotoblastic (82% to 90%, 55% to 66%, and 48% to 56% germination under 12-, 24-, and 0-h photoperiod, respectively) and germinated over a wide range of temperatures (5 to 40 C). Seedlings emerged from all seed burial depths (0 to 15 cm); however, germination was drastically reduced (>90% reduction) beyond 6 to 8 cm. Seed germination was 30% to 38% for seeds placed on the surface and increased to 78% to 88% for seeds buried 2 cm deep, while germination was severely reduced for seeds buried 10 and 15 cm deep (8 to 12% and 0 to 6%, respectively). Seeds of all populations were found to be sensitive to osmotic and salinity stress and to highly acidic and alkaline pH levels. All populations had similar requirements for germination that are directly related to their area of naturalization. These results indicate that the species has limited potential for range expansion to arid, semiarid (nonirrigated), and relatively saline areas. However, humid, salinity-free, and frequently irrigated areas of the country are at high risk of invasion. In arable lands, deep tillage followed by shallow tillage and effective management of emerging seedlings could deplete the soil seedbank in the long run. However, there is an urgent need for effective strategies to manage the species in other nonarable areas.

Cover crop–based, organic rotational no-till (CCORNT) corn and soybean systems have been developed in the mid-Atlantic region to build soil health, increase management flexibility, and reduce labor. In this system, a roller-crimped cover crop mulch provides within-season weed suppression in no-till corn and soybean. A cropping system experiment was conducted in Pennsylvania, Maryland, and Delaware to test the cumulative effects of a multitactic weed management approach in a 3-yr hairy vetch/triticale–corn–cereal rye–soybean–winter wheat CCORNT rotation. Treatments included delayed planting dates (early, intermediate, late) and supplemental weed control using high-residue (HR) cultivation in no-till corn and soybean phases. In the no-till corn phase, HR cultivation decreased weed biomass relative to the uncultivated control by 58%, 23%, and 62% in Delaware, Maryland, and Pennsylvania, respectively. In the no-till soybean phase, HR cultivation decreased weed biomass relative to the uncultivated treatment planted in narrow rows (19 to 38 cm) by 20%, 41%, and 78% in Delaware, Maryland, and Pennsylvania, respectively. Common ragweed was more dominant in soybean (39% of total biomass) compared with corn (10% of total biomass), whereas giant foxtail and smooth pigweed were more dominant in corn, comprising 46% and 22% of total biomass, respectively. Common ragweed became less abundant as corn and soybean planting dates were delayed, whereas giant foxtail and smooth pigweed increased as a percentage of total biomass as planting dates were delayed. At the Pennsylvania location, inconsistent termination of cover crops with the roller-crimper resulted in volunteer cover crops in other phases of the rotation. Our results indicate that HR cultivation is necessary to achieve adequate weed control in CCORNT systems. Integration of winter grain or perennial forages into CCORNT systems will also be an important management tactic for truncating weed seedbank population increases.

Silvery-Thread Moss (Bryum argenteum Hedw.) is an undesirable invader of golf course putting greens across North America, establishing colonies and proliferating despite practices to suppress it. The goal was to grow genotypes of green (growing in putting greens) and native (growing in habitats outside of putting greens) B. argenteum in a common garden experiment, allowing an experimental test of life-history traits between genotypes from these two habitats. Seventeen collections of green and 17 collections of native B. argenteum were cloned to single genotypes and raised through a minimum of two asexual generations in the lab. A culture of each genotype was initiated using a single detached shoot apex and was allowed to grow for 6 mo under conditions of inorganic nutrients present and absent. Compared with genotypes from native habitats, genotypes of B. argenteum from putting greens exhibited earlier shoot regeneration and shoot induction, faster protonemal extension, longer (higher) shoots, lower production of gemmae and bulbils, and greater aerial rhizoid cover, and showed similar tendencies of chlorophyll fluorescence properties and chlorophyll content. Cultures receiving no inorganic nutrients produced less chlorophyll content, greatly reduced growth, and bleaching of shoots. Mosses from putting greens establish more quickly, grow faster, produce more abundant rhizoids, and yet do not produce as many specialized asexual propagules compared with mosses of the same species from native habitats. The highly managed putting green environment has either selected for a suite of traits that allow the moss to effectively compete with grasses, or genotypic diversity is very high in this species, allowing a set of specialized genotypes to colonize the putting green from native habitats. Successful golf course weeds have been able to adapt to this highly competitive environment by selection acting on traits or genotypes to produce plants more successful in competing with golf course grasses.

Halauxifen-methyl is a new synthetic auxin herbicide for control of broadleaf weeds, including preplant applications for corn (Zea mays L.) or soybean [Glycine max (L.) Merr.]. The objective of this study was to investigate the efficacy of halauxifen-methyl in comparison to the current auxin standards, 2,4-D and dicamba, on glyphosate-resistant (GR) horseweed (Erigeron canadensis L.) at different plant heights. In field experiments, a foliar application of halauxifen-methyl at the recommended use rate of 5 g ae ha−1 resulted in 81% control. Dicamba applied at 280 g ae ha−1 provided a comparable level of efficacy of 80%, while 2,4-D at 560 g ae ha−1 resulted in 49% control. The addition of glyphosate improved GR E. canadensis control with 2,4-D more than with halauxifen-methyl or dicamba, possibly due to the higher level of control observed with halauxifen-methyl or dicamba alone. Even though applied at 50 to 100 times lower application rates, the efficacy of halauxifen-methyl on E. canadensis was similar to dicamba and greater than 2,4-D. Thus, halauxifen-methyl should be an effective tool for management of GR E. canadensis before planting both conventional and herbicide-resistant soybean varieties, and it precludes the extended preplant application interval required for dicamba in some soybean management systems.

Fomesafen is a protoporphyrinogen oxidase (PROTOX) inhibitor that has the potential to be used as an alternative mechanism of action for PRE nutsedge and broadleaf weed control in Florida production of small fruit and vegetables. Fumigation in the raised-bed plasticulture system may increase herbicide persistence. Fomesafen persistence could dissuade Florida growers from using the herbicide for fear of injury to subsequent susceptible crops. Field experiments were conducted in Balm, FL, in 2015 and 2016 to investigate the effect of fumigation on fomesafen dissipation, eggplant tolerance, and purple nutsedge control. Treatments included fomesafen at 0.42 kg ai ha−1, S-metolachlor at 1.06 kg ai ha−1, and a nontreated control in either a fumigated bed injected with a combination of 39% 1,3-dichloropropene and 59.6% chloropicrin at 336 kg ha−1 or no fumigant. Fomesafen concentration in the soil decreased by 83% and 96% from application to harvest in 2015 and 2016, respectively. Fumigation did not affect fomesafen dissipation in either year. At 2 wk after transplant (WATr), fomesafen caused 14% eggplant injury. Injury decreased to less than 5% at 6 WATr. Fomesafen and S-metolachlor treatments did not reduce eggplant height or yields compared with the nontreated control. Fumigation and fomesafen did not decrease purple nutsedge density; however, S-metolachlor applications resulted in a 48% reduction. Further research is needed to assess efficacy on broadleaf and grass weeds.

Leafy spurge (Euphorbia esula L.) is an invasive perennial weed infesting range and recreational lands of North America. Previous research and omics projects with E. esula have helped develop it as a model for studying many aspects of perennial plant development and response to abiotic stress. However, the lack of an assembled genome for E. esula has limited the power of previous transcriptomics studies to identify functional promoter elements and transcription factor binding sites. An assembled genome for E. esula would enhance our understanding of signaling processes controlling plant development and responses to environmental stress and provide a better understanding of genetic factors impacting weediness traits, evolution, and herbicide resistance. A comprehensive transcriptome database would also assist in analyzing future RNA-seq studies and is needed to annotate and assess genomic sequence assemblies. Here, we assembled and annotated 56,234 unigenes from an assembly of 589,235 RNA-seq-derived contigs and a previously published Sanger-sequenced expressed sequence tag collection. The resulting data indicate that we now have sequence for >90% of the expressed E. esula protein-coding genes. We also assembled the gene space of E. esula by using a limited coverage (18X) genomic sequence database. In this study, the programs Velvet and Trinity produced the best gene-space assemblies based on representation of expressed and conserved eukaryotic genes. The results indicate that E. esula contains as much as 23% repetitive sequences, of which 11% are unique. Our sequence data were also sufficient for assembling a full chloroplast and partial mitochondrial genome. Further, marker analysis identified more than 150,000 high-quality variants in our E. esula L-RNA–scaffolded, whole-genome, Trinity-assembled genome. Based on these results, E. esula appears to have limited heterozygosity. This study provides a blueprint for low-cost genomic assemblies in weed species and new resources for identifying conserved and novel promoter regions among coordinately expressed genes of E. esula.

Weed management strategies differ in their ability to control weeds, and often have unique agroecological implications. To provide growers with an improved sense of trade-offs between weed control and ecological effects, we implemented several prominent organic weed management strategies in yellow onion in 2014 and 2015. Strategies included cultivation of weed seedlings during the early, weed-sensitive “critical period” of the crop; frequent cultivation events to ensure “zero seed rain”; and weed suppression with polyethylene or natural mulches. As expected, end-of-season weed biomass and weed seed production were greatest in the critical period system and nearly zero for the zero seed rain system. Weeds were also well controlled in natural mulch systems. Average onion yield per treatment was 50.7 Mg ha−1. In 2014, the critical period system and the polyethylene mulch systems demonstrated yield loss, likely due to weed competition and excessive soil temperature, respectively. Onion soluble solids content was also diminished in these systems in 2014, but bulb firmness was greatest in unmulched systems. Carabid beetles, earthworms, soil compaction, soil nitrate, and microbial biomass were affected by weed management strategy, with natural-mulched systems generally performing most favorably. However, these effects were not substantial enough to affect yield of a subsequent sweet corn crop grown in weed-free conditions. In contrast, sweet corn managed with only early-season cultivations demonstrated yield loss (P=0.004) in plots where the critical period treatment was implemented the prior year, indicating that weed competition resulting from abundant weed seed production in that system was the most influential legacy effect of the weed management strategies.

Corn poppy (Papaver rhoeas L.) is the most widespread broadleaf weed species infesting winter cereals in Europe. Biotypes that are resistant to both 2,4-D and tribenuron-methyl, an acetolactate synthase (ALS) inhibitor, have evolved in recent decades, thus narrowing the options for effective chemical control. Though the effectiveness of several integrated weed management (IWM) strategies have been confirmed, none of these strategies have been tested to manage multiple herbicide–resistant P. rhoeas under no-till planting. With the expansion of no-till systems, it is important to prove the effectiveness of such strategies. In this study, a field experiment over three consecutive seasons was conducted to evaluate and compare the effects of different weed management strategies, under either direct drilling (i.e., no-till) or intensive tillage, on a multiple herbicide–resistant P. rhoeas population. Moreover, evaluations were carried out as to whether the proportions of ALS inhibitor–resistant individuals were affected by the tillage systems for each IWM strategy at the end of the 3-yr period. The IWM strategies tested in this research included crop rotation, delayed sowing, and different herbicide programs such as PRE plus POST or POST. All IWM strategies greatly reduced the initial density of P. rhoeas each season (≥ 95%) under either direct drilling or intensive tillage. After 3 yr, the IWM strategies were very effective in both tillage systems, though the effects were stronger under direct drilling (~95%) compared with intensive tillage (~86%). At the end of the study, the proportion of ALS inhibitor–resistant plants was not different between the IWM strategies in both tillage systems (94% on average). Therefore, crop rotation (with sunflower [Helianthus annuus L.]), delayed sowing, or a variation in the herbicide application timing are effective under direct drilling to manage herbicide-resistant P. rhoeas. Adoption of IWM strategies is necessary to mitigate the evolution of resistance in both conventional and no-till systems.

Previous research has shown that strip-tillage (ST) systems conserve soil, reduce production costs, and save time for growers compared with intensive-tillage systems. In contrast to these well-documented benefits, we have limited information on weed community dynamics and management risks in ST corn (Zea mays L.) production systems in the northern Corn Belt. Therefore, we conducted research in 2015 and 2016 to characterize weed community composition, emergence patterns, and aboveground productivity in an ST corn/no-tillage (NT) soybean [Glycine max (L.) Merr.] rotation that was established in 2007 compared with a long-term intensive-tillage chisel-plow (CP) continuous-corn system. Fifteen or more weed species were identified in nontreated quadrats in each cropping system in each year. Common lambsquarters (Chenopodium album L.) was the most abundant weed species across systems and years. Chenopodium album densities were similar between CP and ST corn phases and were approximately 2-fold greater compared with the NT soybean phase. Other abundant weed species occurred at much lower densities than C. album. In each year, cumulative emergence of nontreated weed communities was described best by a logistic function in each cropping system. Maximum weed community emergence was greater in CP corn than ST corn phases in 2015, but did not differ in 2016. In the ST corn phase, most (about 75%) weed community emergence occurred in the in-row (tilled) zone compared with the between-row (nondisturbed) zone. Total late-season weed shoot biomass did not differ between nontreated CP and ST corn phases in either year, with C. album accounting for >85% of total weed biomass in these phases. These results suggest that weed community composition, total emergence, and productivity were similar between CP and ST corn phases after 10 yr. Our findings, coupled with previous research that showed favorable agronomic performance and greater soil conservation associated with the long-term ST corn/NT soybean system, suggest that production risks are no greater than a CP corn system, while processes that underpin ecosystem services are enhanced. These results provide strong evidence to support grower adoption of ST practices as an alternative to intensive tillage.

The potential role of fall-seeded cover crops for weed management in edamame [soybean, Glycine max (L.) Merr.] is unknown. Field experiments were conducted over three edamame growing seasons to (1) determine the extent to which cover crop–residue management systems influence edamame emergence while selectively suppressing weed density and biomass, and (2) determine whether cultivars differed in emergence in cover crop–residue management systems. Cover crop treatments included a winter-killed oilseed radish (Raphanus sativus L.), two canola (Brassica napus L.) treatments (early-killed and late-killed), two cereal rye (Secale cereale L.) treatments (early-killed and late-killed), and a bare-soil control. Two spring timings of a cover crop burndown application created the early-killed and late-killed treatments for canola and cereal rye. Twelve soybean cultivars were tested, including 11 edamame cultivars differing in seed size and a grain-type soybean control. Spring residue biomass in cover crop treatments ranged from 438 kg ha−1 for winter-killed radish to 9,003 kg ha−1 for late-killed cereal rye. Cultivars responded similarly to cover crop treatments, and with the exception of late-killed cereal rye, cover crop treatments resulted in similar crop emergence as the bare-soil control. While all cover crop treatments reduced weed biomass 6 wk after planting compared with the bare soil, winter-killed radish and both canola treatments increased weed density. Early-killed cereal rye has potential for weed management in edamame, as evidenced by the fact that the treatment did not interfere with planting or crop establishment, yet reduced weed density 20% and suppressed early-season weed growth 85%.

Genetically engineered (GE) herbicide-resistant crops have been widely adopted by farmers in the United States and other countries around the world, and these crops have caused significant changes in herbicide use patterns. GE crops have been blamed for increased problems with herbicide-resistant weeds (colloquially called by the misnomer “superweeds”); however, there has been no rigorous analysis of herbicide use or herbicide-resistant weed evolution to quantify the impact of GE crops on herbicide resistance. Here, I analyze data from the International Survey of Herbicide Resistant Weeds and the USDA and demonstrate that adoption of GE corn varieties did not reduce herbicide diversity, and therefore likely did not increase selection pressure for herbicide-resistant weeds in that crop. Adoption of GE herbicide-resistant varieties substantially reduced herbicide diversity in cotton and soybean. Increased glyphosate use in cotton and soybean largely displaced herbicides that are more likely to select for herbicide-resistant weeds, which at least partially mitigated the impact of reduced herbicide diversity. The overall rate of newly confirmed herbicide-resistant weed species to all herbicide sites of action (SOAs) has slowed in the United States since 2005. Although the number of glyphosate-resistant weeds has increased since 1998, the evolution of new glyphosate-resistant weed species as a function of area sprayed has remained relatively low compared with several other commonly used herbicide SOAs.

The semiarid Espinal in central Argentina, being recently transformed from natural semiarid grasslands into agriculture, represents an interesting scenario to understand the early stages of weed community assembly and its relationship with crop identity and management. Our aim was to characterize the weed communities in corn (Zea mays L.) and soybean [Glycine max (L.) Merr.], the main crops of the Espinal region, under the dominant rainfed conditions. Weed surveys were carried out in 53 fields, and farmers were interviewed to collect information about crop management. Floristic composition was compared within and between crops by calculating the additive partition of the abundance-based Bray-Curtis dissimilarity. We compared the frequency and mean cover of functional groups between crops through generalized linear models. Finally, canonical correspondence analysis was carried out to analyze the associations between floristic composition and agronomic variables. Mean alpha and gamma diversity was greater in corn (10.0 and 80 species, respectively) than in soybean (7.6 and 46 species, respectively). Furthermore, species composition of weed communities was more similar among soybean fields than among either cornfields or fields of both crops. Hence, floristic differences between crops are potentially the result of different microenvironmental heterogeneity above- and belowground, with corn likely to be more permissive to weed establishment compared with soybean. The higher frequency of annual, dicotyledonous, and native species, and the high proportion of rare species, mostly native, suggest a strong legacy of the original vegetation that thrived in these recently cultivated systems. The functional composition was also affected by agronomic management, with sulfur, nitrogen, and grass herbicide application being the most important factors related to the floristic composition of weed communities. This early description can be used as a starting point for studies concerning trajectories, mechanisms, and processes of weed communities related to environment and management.

Fomesafen is a protoporphyrinogen oxidase–inhibitor herbicide with an alternative mode of action that provides PRE weed control in strawberry [Fragaria×ananassa (Weston) Duchesne ex Rozier (pro sp.) [chiloensis×virginiana]] produced in a plasticulture setting in Florida. Plasticulture mulch could decrease fomesafen dissipation and increase crop injury in rotational crops. Field experiments were conducted in Balm, FL, to investigate fomesafen persistence and movement in soil in Florida strawberry systems for the 2014/2015 and 2015/2016 production cycles. Treatments included fomesafen preplant at 0, 0.42, and 0.84 kg ai ha−1. Soil samples were taken under the plastic from plots treated with fomesafen at 0.42 kg ha−1 throughout the production cycle. Fomesafen did not injure strawberry or decrease yield. Fomesafen concentration data for the 0.0- to 0.1-m soil depth were described using a three-parameter logistic function. The fomesafen 50% dissipation times were 37 and 47 d for the 2014/2015 and 2015/2016 production cycles, respectively. At the end of the study, fomesafen was last detected in the 0.0- to 0.1-m depth soil at 167 and 194 d after treatment in the 2014/2015 and 2015/2016 production cycles, respectively. Fomesafen concentration was less than 25 ppb on any sampling date for 0.1- to 0.2-m and 0.2- to 0.3-m depths. Fomesafen concentration decreased significantly after strawberry was transplanted and likely leached during overhead and drip irrigation used during the crop establishment.

Broadleaf species escape current integrated weed management strategies in strawberry [Fragaria×ananassa (Weston) Duchesne ex Rozier (pro sp.) [chiloensis×virginiana]] production. Clopyralid is a registered POST control option, but current application timings provide suppression of only some species. Earlier clopyralid application timings may increase spray coverage to weeds at the planting hole, but strawberry plant tolerance to applications shortly after transplant is unknown. The objectives of the study were to determine the degree of clopyralid tolerance when applied to mature strawberry plants according to current management strategies, whether clopyralid absorption and translocation were involved in the tolerance response demonstrated by mature strawberry plants, and whether clopyralid could be safely applied to immature strawberry plants shortly after transplant. Clopyralid caused no damage when applied to mature strawberry plants and did not affect crop height, number of crowns, flowers, immature berries, or yield. Maximal strawberry absorption of radiolabeled clopyralid was 82% of the recovered radioactivity and reached peak (90%) absorption at 15 h. Maximal total translocation of radioactivity from the treated leaf was 17% and reached peak translocation at 52 h. Translocation was primarily to the new leaves and reproductive structures. In the early-application experiment, damage induced by clopyralid for all application timings reached 0 by 8 wk after treatment. Across all timings, maximal damage at 140 g ha−1 was 17% when applied 14 d after transplant (DATr) and 56% at 28 g ha−1 when applied at 21 DATr. Clopyralid dose did not affect the number of crowns, aboveground biomass, or yield. There was some stunting in plant height (3%) by the high labeled dose of clopyralid. Labeled dose clopyralid applications appear safe for application timings closer to strawberry transplant, though considerations of leaf cupping should be taken under consideration for label changes.

Incorporation of cover crop residue into the soil has been suggested as a means for reducing weed seedbanks. To explore this hypothesis, we buried mesh bags of seeds mixed with sand at 15-cm depth in late fall in plots that had been planted with rye (Secale cereale L.) or hairy vetch (Vicia villosa Roth.) or left unplanted. Separate bags contained either velvetleaf (Abutilon theophrasti Medik.), giant foxtail (Setaria faberi Herrm.), Powell amaranth (Amaranthus powellii S. Watson), or common lambsquarters (Chenopodium album L.). The experiment used a randomized complete block design with five replications, and enough bags were buried to allow a final recovery in each of the following three springs. Each spring, bags were exhumed, and seeds were either counted and tested for viability or mixed with chopped cover crop material or simply stirred for control bags, and the material was reburied. The experiment was completed twice with initial burials in fall of 2011 and 2013. Rye had no consistent effect on persistence of seeds of any of the species. For two observation intervals, rye increased persistence of a species; for another two intervals, it decreased persistence relative to the control; but mostly rye did not affect persistence. Hairy vetch decreased persistence of C. album and A. powellii in both runs of the experiment but had no effect on persistence of A. theophrasti and S. faberi. Germination of the first two species is promoted by nitrate, whereas A. theophrasti germination is not sensitive to nitrate, and S. faberi is only rarely nitrate sensitive. We suggest that nitrate released during decomposition of hairy vetch may have promoted fatal germination of C. album and A. powellii. Incorporation of legume cover crops like hairy vetch may provide a means for decreasing the seedbanks of the many weed species whose germination is promoted by nitrate. The lack of any reduction of A. theophrasti and S. faberi seed persistence in response to hairy vetch and the inconsistent and mostly negligible effect of rye indicate that a general increase in readily decomposable organic matter through incorporation of cover crops may be ineffective at reducing weed seedbanks.

The development of early-maturing soybean [Glycine max (L.) Merr.] varieties has led to an increase in soybean production in canola (Brassica napus L.)-dominant crop rotations in western Canada. Herbicide-resistant (HR) volunteer B. napus can be difficult to manage in HR soybean using herbicides alone. In 2013 and 2014, four field experiments were conducted in Manitoba, Canada, to evaluate soybean row spacing, seeding density, nitrogen supply, and interrow tillage as candidate nonchemical weed management tools for an integrated program to manage volunteer B. napus in soybean. Among treatments and sites, volunteer B. napus produced about 830 seeds plant−1 and resulted in large seedbank inputs (averaging about 20,300 seeds m−2). Volunteer B. napus seedling recruitment differed among sites, and resulted in two distinct classes of sites based on average seedling densities of 39 and 89 plants m−2. Weed management tools were more effective at the sites with higher volunteer B. napus densities. At these sites, soybean yield was greater when using an increased soybean-seeding density (44% greater yield using a seeding density of 682,500 vs. 455,000 seeds ha−1) or interrow tillage (36% greater yield with vs. without using interrow tillage). Soybean row spacing (19 vs. 38 vs. 76 cm) did not affect soybean yield, unless the reduction in row spacing was combined with an increased seeding density (65% greater yield with narrow-row soybean seeded at 682,500 vs. wide-row soybean seeded at 455,000 seeds ha−1). At the sites with higher volunteer B. napus densities, seed production of canola volunteer B. napus was greater when nitrogen fertilizer was applied to simulate an environment with greater nitrogen supply (77% greater number of volunteer B. napus seeds produced with vs. without broadcast application of 23 kg N ha−1 urea). In northern climates, seeding soybean at increased densities using narrow-row spacing in fields with limited soil inorganic nitrogen and using interrow tillage in wide-row production systems are effective strategies that could augment chemical weed management in an integrated program for management of volunteer B. napus, and perhaps also other competitive early-season weeds.

Interest in planting mixtures of cover crop species has grown in recent years as farmers seek to increase the breadth of ecosystem services cover crops provide. As part of a multidisciplinary project, we quantified the degree to which monocultures and mixtures of cover crops suppress weeds during the fall-to-spring cover crop growing period. Weed-suppressive cover crop stands can limit weed seed rain from summer- and winter-annual species, reducing weed population growth and ultimately weed pressure in future cash crop stands. We established monocultures and mixtures of two legumes (medium red clover and Austrian winter pea), two grasses (cereal rye and oats), and two brassicas (forage radish and canola) in a long fall growing window following winter wheat harvest and in a shorter window following silage corn harvest. In fall of the long window, grass cover crops and mixtures were the most weed suppressive, whereas legume cover crops were the least weed suppressive. All mixtures also effectively suppressed weeds. This was likely primarily due to the presence of fast-growing grass species, which were effective even when they were seeded at only 20% of their monoculture rate. In spring, weed biomass was low in all treatments due to winter kill of summer-annual weeds and low germination of winter annuals. In the short window following silage corn, biomass accumulation by cover crops and weeds in the fall was more than an order of magnitude lower than in the longer window. However, there was substantial weed seed production in the spring in all treatments not containing cereal rye (monoculture or mixture). Our results suggest that cover crop mixtures require only low seeding rates of aggressive grass species to provide weed suppression. This creates an opportunity for other species to deliver additional ecosystem services, though careful species selection may be required to maintain mixture diversity and avoid dominance of winter-hardy cover crop grasses in the spring.

The triazines are one of the most widely used herbicide classes ever developed and are critical for managing weed populations that have developed herbicide resistance. These herbicides are traditionally valued for their residual weed control in more than 50 crops. Scientific literature suggests that atrazine, and perhaps other s-triazines, may no longer remain persistent in soils due to enhanced microbial degradation. Experiments examined the rate of degradation of atrazine and two other triazine herbicides, simazine and metribuzin, in both atrazine-adapted and non-history Corn Belt soils, with similar soils being used from each state as a comparison of potential triazine degradation. In three soils with no history of atrazine use, the t1/2 of atrazine was at least four times greater than in three soils with a history of atrazine use. Simazine degradation in the same three sets of soils was 2.4 to 15 times more rapid in history soils than non-history soils. Metribuzin in history soils degraded at 0.6, 0.9, and 1.9 times the rate seen in the same three non-history soils. These results indicate enhanced degradation of the symmetrical triazine simazine, but not of the asymmetrical triazine metribuzin.

Research was conducted from 2015 to 2017 to investigate the potential for 2,4-D and multiple herbicide resistance in a waterhemp [Amaranthus tuberculatus (Moq.) J. D. Sauer] population from Missouri (designated MO-Ren). In the field, visual control of the MO-Ren population with 0.56 to 4.48 kg 2,4-D ha−1 ranged from 26% to 77% in 2015 and from 15% to 55% in 2016. The MO-Ren population was highly resistant to chlorimuron, with visual control never exceeding 7% either year. Estimates of the 2,4-D dose required to provide 50% visual control (I50) of the MO-Ren population were 1.44 kg ha−1 compared with only 0.47 kg 2,4-D ha−1 for the susceptible population. Based on comparisons to a susceptible population in dose–response experiments, the MO-Ren population was approximately 3-fold resistant to 2,4-D, and 7-, 7-, 22-, and 14-fold resistant to atrazine, fomesafen, glyphosate, and mesotrione, respectively. Dicamba and glufosinate were the only two herbicides that provided effective control of the MO-Ren population in these experiments. Examinations of multiple herbicide resistance at the individual plant level revealed that 16% of the plants of the MO-Ren population contained genes stacked for six-way herbicide resistance, and only 1% of plants were classified as resistant to a single herbicide (glyphosate). Results from these experiments confirm that the MO-Ren A. tuberculatus population is resistant to 2,4-D, atrazine, chlorimuron, fomesafen, glyphosate, and mesotrione, making this population the third 2,4-D–resistant A. tuberculatus population identified in the United States, and the first population resistant to six different herbicidal modes of action.