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Cytotoxic properties of graphene derivatives depending on origin and type of cell line

Published online by Cambridge University Press:  05 August 2020

Agnieszka Zuchowska Open the ORCID record for Agnieszka Zuchowska [Opens in a new window] ,
Bartlomiej Dabrowski ,
Elzbieta Jastrzebska ,
Marta Mazurkiewicz-Pawlicka ,
Artur Malolepszy ,
Leszek Stobinski ,
Maciej Trzaskowski  and
Zbigniew Brzozka
Agnieszka Zuchowska*
Affiliation:
Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 00-664Warsaw, Poland
Bartlomiej Dabrowski
Affiliation:
Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 00-664Warsaw, Poland
Elzbieta Jastrzebska
Affiliation:
Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 00-664Warsaw, Poland
Marta Mazurkiewicz-Pawlicka
Affiliation:
Graphene Laboratory of Warsaw University of Technology, Faculty of Chemical and Process Engineering, Warsaw University of Technology, 00-645Warsaw, Poland
Artur Malolepszy
Affiliation:
Graphene Laboratory of Warsaw University of Technology, Faculty of Chemical and Process Engineering, Warsaw University of Technology, 00-645Warsaw, Poland
Leszek Stobinski
Affiliation:
Graphene Laboratory of Warsaw University of Technology, Faculty of Chemical and Process Engineering, Warsaw University of Technology, 00-645Warsaw, Poland
Maciej Trzaskowski
Affiliation:
Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, 02-822Warsaw, Poland
Zbigniew Brzozka
Affiliation:
Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 00-664Warsaw, Poland
*
a)Address all correspondence to this author. e-mail: azuchowska@ch.pw.edu.pl
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Abstract

This work is focused on determining whether two graphene derivatives: graphene oxide (GO) and reduced graphene oxide (RGO) can be used alone as a component of anticancer therapy. In this paper, we present the synthesis GO and RGO, their physicochemical characterization as well as an evaluation of their cytotoxic properties on cancer (HepG2 and MCF-7) and non-malignant (clone-9 and HMF) cells. We demonstrated that both tested graphene derivatives have a high affinity to cancer cells. We showed that cytotoxic properties of GO and RGO were different depending on the type of solvent in which they were prepared. Additionally, we observed that cytotoxic properties of GO and RGO were different depending on the origin of the cells (liver and breast) and the form of graphene material (GO and RGO). We showed that GO and RGO can be potential, selectively materials which in future can found application in anticancer therapy.

Keywords

graphene oxidereduced graphene oxidecytotoxicitycancer researchgraphene
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 18 , 28 September 2020 , pp. 2385 - 2395
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Copyright
Copyright © Materials Research Society 2020

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References

Zakharian, T.Y., Seryshev, A., Sitharaman, B., Gilbert, B.E., Knight, V., and Wilson, L.J.: A fullerene−paclitaxel chemotherapeutic: Synthesis, characterization, and study of biological activity in tissue culture. J. Am. Chem. Soc. 127, 12508 (2005).CrossRefGoogle ScholarPubMed
Liu, Z., Tabakman, S., Welsher, K., and Dai, H.: Carbon nanotubes in biology and medicine: In vitro and in vivo detection, imaging and drug delivery. Nano Res. 2, 85 (2009).CrossRefGoogle ScholarPubMed
Pinto, A.M., Gonçalves, I.C., and Magalhães, F.D.: Graphene-based materials biocompatibility: A review. Colloids Surf. B 111, 188 (2013).10.1016/j.colsurfb.2013.05.022CrossRefGoogle ScholarPubMed
Bitounis, D., Boucetta, A., Hong, B.H., Min, D.H., and Kostarelos, K.: Prospects and challenges of graphene in biomedical applications. Adv. Mater. 25, 2258 (2013).CrossRefGoogle ScholarPubMed
Markovic, Z.M., Harhaji-Trajkovic, L.M., Todorovic-Markovic, B.M., Kepi, D.P., Arsikin, K.M., Jovanovi, S.P., Pantovic, A.C., Dramicanin, M.D., and Trajkovic, V.S.: In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes. Biomaterials 32, 1121 (2011).CrossRefGoogle ScholarPubMed
Robinson, J.T., Tabakman, S.M., Liang, Y., Wang, H., Casalongue, H.S., Vinh, D., and Dai, H.: Ultrasmall reduced graphene oxide with high near-infrared absorbance for photothermal therapy. J. Am. Chem. Soc. 133, 6825 (2011).CrossRefGoogle ScholarPubMed
Huang, P., Xu, C., Lin, J., Wang, C., Wang, X., Zhang, C., Zhou, X., Guo, S., and Cui, D.: Folic acid-conjugated graphene oxide loaded with photosensitizers for targeting photodynamic therapy. Theranostics 1, 240 (2011).10.7150/thno/v01p0240CrossRefGoogle ScholarPubMed
Tian, B., Wang, C., Zhang, S., Feng, L., and Liu, Z.: Photothermally enhanced photodynamic therapy delivered by nano-graphene oxide. ASC Nano 5, 7000 (2011).CrossRefGoogle ScholarPubMed
Zhang, L., Lu, Z., Zhao, Q., Huang, J., Shen, H., and Zhang, Z.: Enhanced chemotherapy efficacy by sequential delivery of siRNA and anticancer drugs using PEI-grafted graphene oxide. Small 7, 460 (2011).CrossRefGoogle ScholarPubMed
Xu, Z., Wang, S., Li, Y., Wang, M., Shi, P., and Huang, X.: Covalent functionalization of graphene oxide with biocompatible poly(ethylene glycol) for delivery of paclitaxel. ACS Appl. Mater. Interfaces 6, 17268 (2014).10.1021/am505308fCrossRefGoogle ScholarPubMed
Bengtson, S., Kling, K., Madsen, A.M., Noergaard, A.W., Jacobsen, N.R., Clausen, P.A., Alonso, B., Pesquera, A., Zurutuz, A., Ramos, R., Okuno, H., Dijon, J., Wallin, H., and Vogel, U.: No cytotoxicity or genotoxicity of graphene and graphene oxide in murine lung epithelial FE1 cells in vitro. Environ. Mol. Mutagen. 57, 469 (2016).10.1002/em.22017CrossRefGoogle ScholarPubMed
Loutfy, S.A., Salaheldin, T.A., Ramadan, M.A., Farroh, K.Y., Abdallah, Z.F., and Youssef, T.: Synthesis, characterization and cytotoxic evaluation of graphene oxide nanosheets: In vitro liver cancer model. Asian Pac. J. Cancer Prev. 18, 955 (2017).Google ScholarPubMed
Wu, J., Yang, R., Zhang, L., Fan, Z., and Liu, S.: Cytotoxicity effect of graphene oxide on human MDA-MB-231 cells. Toxicol. Mech. Methods 25, 312 (2015).CrossRefGoogle ScholarPubMed
Tabish, T.A., Pranjol, M.Z., Hayat, H., Rahat, A.A.M., Abdullah, T.M., Whatmore, J.L., and Zhang, S.: In vitro toxic effects of reduced graphene oxide nanosheets on lung cancer cells. Nanotechnology 15, 504001 (2017).CrossRefGoogle Scholar
Luo, L., Xu, L., and Zhao, H.: Biosynthesis of reduced graphene oxide and its in-vitro cytotoxicity against cervical cancer (HeLa) cell lines. Mater. Sci. Eng. C 78, 198 (2017).CrossRefGoogle ScholarPubMed
Zhou, T., Zhang, B., Wei, P., Du, Y., Zhou, H., Yu, M., Yan, L., Zhang, W., Nie, G., Chen, C., Tu, Y., and Wei, T.: Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets. Biomaterials 37, 9833 (2014).CrossRefGoogle Scholar
Yeh, C.N., Raidongia, K., Shao, J., Yang, Q.H., and Huang, J.: On the origin of the stability of graphene oxide membranes in water. Nat. Chem. 7, 166 (2015).CrossRefGoogle Scholar
Li, D., Müller, M.B., Gilje, S., Kaner, R.B., and Wallace, G.G.: Processable aqueous dispersions of graphene nanosheets. Nat. Nanotechnol. 3, 101 (2008).CrossRefGoogle ScholarPubMed
Gurunathan, S., Han, J.W., Eppakayala, V., and Kim, J.H.: Green synthesis of graphene and its cytotoxic effects in human breast cancer cells. J. Nanomed. 8, 1015 (2013).10.2147/IJN.S42047CrossRefGoogle ScholarPubMed
Chaudhari, N.S., Pandey, A.P., Patil, P.O., Tekade, A.R., Bari, S.B., and Deshmukh, P.K.: Graphene oxide based magnetic nanocomposites for efficient treatment of breast cancer. Mater. Sci. Eng. C 37, 278 (2014).CrossRefGoogle ScholarPubMed
Chatterjee, N., Eom, H.J., and Choi, J.: A systems toxicology approach to the surface functionality control of graphene-cell interactions. Biomaterials 35, 1109 (2014).CrossRefGoogle ScholarPubMed
Das, S., Singh, S., Singh, V., Joung, D., Dowding, J.M., Reid, D., Anderson, J., Zhai, L., Khondaker, S.I., Self, W.T., and Seal, S.: Oxygenated functional group density on graphene oxide: Its effect on cell toxicity. Part. Part. Syst. Charact. 30, 148 (2013).CrossRefGoogle Scholar
Vallabani, N.V., Mittal, S., Shukla, R.K., Pandey, A.K., Dhakate, S.R., Pasricha, R., and Dhawan, A.: Toxicity of graphene in normal human lung cells (BEAS-2B). J. Biomed. Nanotechnol. 7, 106 (2011).CrossRefGoogle Scholar
Wang, A., Pu, K., Dong, B., Liu, Y., Zhang, L., Zhang, Z., Duan, W., and Zhu, Y.: Role of surface charge and oxidative stress in cytotoxicity and genotoxicity of graphene oxide towards human lung fibroblast cells. J Appl. Toxicol. 33, 1156 (2013).10.1002/jat.2877CrossRefGoogle ScholarPubMed
Fulda, S., Gorman, A.M., Hori, O., and Samali, A.: Cellular stress responses: Cell survival and cell death. Int. J. Cell Biol. (2010). doi:10.1155/2010/214074.CrossRefGoogle ScholarPubMed
Sanchez, V.C., Jachak, A., Hurt, R.H., and Kane, A.B.: Biological interactions of graphene-family nanomaterials – An interdisciplinary review. Chem. Res. Toxicol. 25, 15 (2012).CrossRefGoogle ScholarPubMed
Fletcher, A.: The cell membrane and receptors. Anaesth. Intensive Care Med. 18, 316 (2017).CrossRefGoogle Scholar
Hummers, W.S. and Offeman, R.E.: Preparation of graphitic oxide. J. Am. Chem. Soc. 80, 1339 (1958).CrossRefGoogle Scholar
Zhang, J., Yang, H., Shen, G., Cheng, P., Zhang, J., and Guo, S.: Reduction of graphene oxide via L-ascorbic acid. Chem. Commun. 46, 1112 (2010).CrossRefGoogle ScholarPubMed