The Diptera fauna from the late Eocene of the Isle of Wight (Bembridge Marls) is studied including redescriptions of formerly described material. The fauna includes the following taxa: Anisopodidae – one species; Bibionidae – 11 species; Ceratopogonidae – one described and two unidentified species; Chironomidae – undetermined species of three subfamilies; Culicidae – four species; Cylindrotomidae – one species; Dixidae – one species; Keroplatidae – one described and four unidentified species; Limoniidae – 31 species; Mycetophilidae – 14 species; Psychodidae – two species; Scatopsidae – two species; Sciaridae – three species; Simuliidae – an unnamed species; Tipulidae – nine species. Diptera Brachycera: Agromyzidae – unidentified species; Anthomyzidae – two species; Asilidae – two species; Bombyliidae – one species; Chloropidae – one species; Dolichopodidae – unidentified species; Empididae – unidentified species; Ephydridae – two species; Heleomyzidae – unidentified species; Lauxaniidae – unidentified species; Otitidae – one species; Phoridae – unidentified species; Rhagionidae – two species; Stratiomyidae – two species; Syrphidae – unidentified species; Tabanidae – one species. Following new taxa are described: Leptotarsus (Longurio) fragmentatus Krzemiński, n. sp., Leptotarsus (Longurio) wegiereki Krzemiński, n. sp., Tipula (s. lato) anglicana Krzemiński, n. sp. (Tipulidae); Cyttaromyiarossi Krzemiński, n. sp. (Cylindrotomidae); Gurnardia corami Krzemiński, n. gen, n. sp., Pilaria hooleyi Krzemiński, n. sp., Pilaria volodii Krzemiński, n. sp., Cheilotrichia (Cheilotrichia) duplicata Krzemiński, n. sp., Cheilotrichia (Empeda) szwedoi Krzemiński, n. sp., Symplecta (Psiloconopa) gurnetensis Krzemiński, n. sp., Orimarga (Orimarga) lenae Krzemiński, n. sp., Dicranomyia (Dicranomyia) azari Krzemiński, n. sp., Dicranomyia (Dicranomyia) fasciata Krzemiński, n. sp., Helius (Helius) edmundi Krzemiński, n. sp., Helius (Helius) popovi Krzemiński, n. sp., Dicranoptycha staryi Krzemiński, n. sp. (Limoniidae); Wightipsychoda Azar, n. gen. (Psychodidae); Dilophus andrewrossi Nel, Colomb & Waller, n. sp., Bibiodes massiliensis Nel, Colomb & Waller, n. sp. (Bibionidae); Azana cockerelli Blagoderov, n. sp., Aglaomyia vectis Blagoderov, n. sp., Dziedzickia oligocenica Blagoderov, n. sp., Palaeoempalia saxea Blagoderov, n. sp., Mycomyahoolei Blagoderov, n. sp., Leia gurnardensis Blagoderov, n. sp. (Mycetophilidae); Regmocleminahaennii Krzemiński, n. sp. (Scatopsidae); Sylvicola problematica Krzemiński, n. sp. (Anisopodidae); Palaeoberidops barkeri Nicholson, n. gen., n. sp. (Stratiomyidae). The Diptera fauna and palaeohabitat of the Insect Bed is discussed.