Direct numerical simulations of the turbulence of a Herschel–Bulkley (HB) fluid in a rough channel are performed at a shear Reynolds number $Re_{\tau } \approx 300$ and a Bingham number ${Bn} \approx 0.9$. For the type of rough surface used in this study, the results indicate that Townsend's wall similarity hypothesis also holds for HB fluids. However, there are notable differences compared with the effect of roughness on Newtonian fluids. More specifically, the effect of roughness appears to be slightly stronger for HB fluids, in the sense that the bulk Reynolds number, based on the viscosity at the wall, is reduced further due to the increase in viscosity in the troughs of the roughness surface induced by the low shear. At the same time, for the simulated rough surface, the contribution of form drag to the total pressure drop is reduced from 1/4 to about 1/5 due to the persistence of viscous shear in the boundary layer, reducing its shielding effect. As for the friction factor, due to the nonlinearity of the HB constitutive relation, its use with the wall shear rate from the mean wall shear stress underpredicts the minimum viscosity at the wall by up to 18 %. This inevitably leads to uncertainties in the prediction of the friction factor. Finally, it is observed that the rough surface is unable to break the peculiar near-wall flow structure of HB fluids, which consists of long persistent low-speed streaks occupying the entire domain. This means that the small-scale energy is significantly reduced for HB fluids, even in rough channels, with the energy more concentrated in the lower wavenumber range, implying an increase in the slope of the power spectrum to $-7/2$ in the inertial range, as shown by Mitishita et al. (J. Non-Newtonian Fluid Mech., vol. 293, 2021, 104570).

Herbicides have been placed in global Herbicide Resistance Action Committee (HRAC) herbicide groups based on their sites of action (e.g., acetolactate synthase–inhibiting herbicides are grouped in HRAC Group 2). A major driving force for this classification system is that growers have been encouraged to rotate or mix herbicides from different HRAC groups to delay the evolution of herbicide-resistant weeds, because in theory, all active ingredients within a herbicide group physiologically affect weeds similarly. Although herbicide resistance in weeds has been studied for decades, recent research on the biochemical and molecular basis for resistance has demonstrated that patterns of cross-resistance are usually quite complicated and much more complex than merely stating, for example, a certain weed population is Group 2-resistant. The objective of this review article is to highlight and describe the intricacies associated with the magnitude of herbicide resistance and cross-resistance patterns that have resulted from myriad target-site and non–target site resistance mechanisms in weeds, as well as environmental and application timing influences. Our hope is this review will provide opportunities for students, growers, agronomists, ag retailers, regulatory personnel, and research scientists to better understand and realize that herbicide resistance in weeds is far more complicated than previously considered when based solely on HRAC groups. Furthermore, a comprehensive understanding of cross-resistance patterns among weed species and populations may assist in managing herbicide-resistant biotypes in the short term by providing growers with previously unconsidered effective control options. This knowledge may also inform agrochemical company efforts aimed at developing new resistance-breaking chemistries and herbicide mixtures. However, in the long term, nonchemical management strategies, including cultural, mechanical, and biological weed management tactics, must also be implemented to prevent or delay increasingly problematic issues with weed resistance to current and future herbicides.

Drip irrigation and mulching were tested to minimize unproductive water loss through evaporation and weed interference. A field experiment was conducted during spring season of 2020 and 2021 in split plot design with three replications. The study includes six treatment combinations of drip irrigation methods (surface drip and subsurface drip irrigation) and mulching (black plastic, paddy straw and no mulch) along with one conventional furrow irrigation without mulching (as control) in main plots. Four weed control treatments (atrazine 1000 g a.i./ha as pre-emergence, two hand weedings at 30 and 60 days after sowing [DAS], weed free and weedy for whole crop growth period) were kept in the subplots. The combination of drip irrigation and mulches significantly enhanced leaf area index and crop biomass at 60 DAS than furrow irrigation. Integration of subsurface drip irrigation with plastic mulching resulted in the lowest weed density and biomass among main plots. Drip irrigation coupled with plastic and straw mulching resulted in 86 and 50% reduction in weed density and biomass, respectively, as compared to no mulching. Integration of subsurface drip with paddy straw mulch and black plastic mulch resulted in 17.1 and 15.5% higher maize grain yield, respectively, as compared to furrow irrigation. The highest irrigation water productivity (3.58 kg/m3) was observed in combination of subsurface drip and paddy straw mulch followed by combination of subsurface drip and black plastic mulch (3.51 kg/m3). Overall, straw mulching in drip irrigation system proved economical in terms of maize productivity.

Volunteer corn (Zea mays L.) is a competitive weed in corn-based cropping systems. Scientific literature does not exist about the water use of volunteer corn grown in different crops and irrigation systems. The objectives of this study were to characterize the growth and evapotranspiration (ETa) of volunteer corn in corn, soybean [Glycine max (L). Merr.], and sorghum [Sorghum bicolor (L.) Moench] under center-pivot irrigation (CPI) and subsurface drip irrigation (SDI) systems. Field experiments were conducted in south-central Nebraska in 2021 and 2022. Soil moisture sensors were installed at depths of 0 to 0.30, 0.30 to 0.60, and 0.60 to 0.90 m to track soil water balance and quantify seasonal total ETa. Corn was the most competitive, as volunteer corn had the lowest biomass, leaf area, and plant height compared with the fallow. Soybean was the least competitive with volunteer corn, as the plant height, biomass, and leaf area of volunteer corn in soybean were similar to fallow at 15, 30, 45, and 60 d after transplanting (DATr). Averaged across crop treatments, irrigation type did not affect volunteer corn growth at 15 to 45 DATr. Soil water depletion and ETa were similar across crop treatments with and without volunteer corn, as water was not a limiting factor in this study. The ETa of volunteer corn was the highest in soybean (623 mm), followed by sorghum (622 mm), and corn (617 mm) under CPI. The SDI had higher irrigation efficiency, because without affecting crop yield, it had 3%, 6%, and 8% lower ETa in soybean (605 mm), sorghum (585 mm), and corn (571 mm), respectively. Although soil water use did not differ with volunteer corn infestation, a soybean yield loss of 27% was observed, which suggests that volunteer corn may not compete for moisture under fully irrigated conditions; however, it can impact the crop yield potential due to competition for factors other than soil moisture.

Benghal dayflower and sicklepod are weeds of economic importance in peanut in the southeastern United States due to their extended emergence pattern and limited effective herbicides for control. Field studies were conducted near Jay, Florida, in 2022 and 2023, to evaluate the effect of planting date and herbicide combinations on Benghal dayflower and sicklepod control in peanut crops. Peanut planted in June was exposed to a higher Benghal dayflower density than peanut planted in May. Sicklepod density was similar between May and June planting dates at 28 d after preemergence and early postemergence herbicide applications, but density was greater in peanut that was planted in June, 28 d after the mid-postemergence application. A preemeergence herbicide application followed by (fb) an early postemergence application of S-metolachlor or diclosulam + S-metolachlor controlled Benghal dayflower 84% to 93% 28 d after early postemergence in peanut that was planted in May, but control was reduced to 58% to 78% in the crop that had been planted in June. Regardless of planting date, a preemeergence application fb S-metolachlor or diclosulam + S-metolachlor applied early postemergence provided <80% sicklepod control 28 d after early postemergence. Imazapic + dimethenamid-P + 2,4-DB applied postemergence improved Benghal dayflower control to at least 94% 28 d after mid-postemergence, but sicklepod control was not >85%. Regardless of the planting date, paraquat + bentazon + S-metolachlor applied early postemergence was required to achieve ≥95% sicklepod control. However, herbicide combinations that included paraquat + bentazon + S-metolachlor reduced peanut yield when planting was delayed to June. In fields that are infested with Benghal dayflower and sicklepod, it is recommended that peanut be planted in early May to minimize the potential impact of these weeds and to increase peanut yield. Late-planted peanut required more intensive herbicide applications to obtain the same peanut yield as the May-planted peanut.

This manuscript addresses a critical topic: navigating complexities of conducting clinical trials during a pandemic. Central to this discussion is engaging communities to ensure diverse participation. The manuscript elucidates deliberate strategies employed to recruit minority communities with poor social drivers of health for participation in COVID-19 trials. The paper adopts a descriptive approach, eschewing analysis of data-driven efficacy of these efforts, and instead provides a comprehensive account of strategies utilized. The Accelerate COVID-19 Treatment Interventions and Vaccines (ACTIV) public–private partnership launched early in the COVID-19 pandemic to develop clinical trials to advance SARS-CoV-2 treatments. In this paper, ACTIV investigators share challenges in conducting research during an evolving pandemic and approaches selected to engage communities when traditional strategies were infeasible. Lessons from this experience include importance of community representatives’ involvement early in study design and implementation and integration of well-developed public outreach and communication strategies with trial launch. Centralization and coordination of outreach will allow for efficient use of resources and the sharing of best practices. Insights gleaned from the ACTIV program, as outlined in this paper, shed light on effective strategies for involving communities in treatment trials amidst rapidly evolving public health emergencies. This underscores critical importance of community engagement initiatives well in advance of the pandemic.

We have established trophoblast cell lines, from parthenogenesis-derived buffalo blastocysts. The buffalo trophoblast cells were cultured continuously over 200 days and 21 passages. These cells were observed by phase-contrast microscopy for their morphology and characterized by reverse transcriptase polymerase chain reaction and immunofluorescence against trophoblast-specific markers and cytoskeletal proteins. Trophoblast cells showed positive staining for CDX2, a marker of these cells at both blastocyst and cell line levels. Epithelial morphology of these cells was revealed by positive staining against cytokeratins and tubulin but not against vimentin and dolichos biflorus agglutinin. Gene expression profiles of many important placenta-specific genes were studied in the primary trophectoderm outgrowths, which were collected on days 0, 5, 9, 12 and 15 of culture and trophoblast cell line at passages 12–15. Therefore, the trophoblast cell line derived can potentially be used for in vitro studies on buffalo embryonic development.

Sicklepod is one of the most difficult to control weeds in peanut production in the southeastern United States due to its extended emergence pattern and limited effective herbicides for control. Growers rely on preemergence herbicides as the foundation of their weed control programs; however, postemergence herbicides are often needed for season-long weed control. The objectives of this study were to evaluate the effect of planting pattern and herbicide combinations for sicklepod control in peanut crops. Due to rapid canopy closure, twin-row planting improved late-season sicklepod control by 13% and peanut yield by 5% compared with a single-row pattern. A preemergence application of fluridone, flumioxazin, or fluridone + flumioxazin provided 76% to 89% control of sicklepod 28 d after preemergence. Regardless of the herbicide applied preemergence, paraquat + bentazon + S-metolachlor applied early postemergence was required to achieve ≥90% sicklepod control 28 d after early postemergence. All preemergence herbicide treatments followed by (fb) S-metolachlor or diclosulam + S-metolachlor applied early postemergence provided <90% control 28 d after early postemergence. A mid-postemergence application of imazapic + dimethenamid-P + 2,4-DB controlled sicklepod by 67% to 79% prior to peanut harvest, and biomass reduction was unacceptable (<80%), resulting in difficulty in peanut digging. The highest peanut yield was observed when paraquat + bentazon + S-metolachlor was applied early postemergence fb imazapic + dimethenamid-P + 2,4-DB applied mid-postemergence. Based on the results of this study, a herbicide combination of paraquat + bentazon + S-metolachlor is an important early-season tool for controlling sicklepod in peanut crops. The results also showed that a twin-row planting pattern improved late-season sicklepod control but did not reduce herbicide input to protect peanut yield.

Twelve lacustrine sediment samples from a relict lake in the Kalla Glacier valley were co-dated using AMS radiocarbon (14C) and infrared stimulated luminescence (IRSL) dating methods. In general, the radiocarbon ages of bulk organic matter were older by a minimum of 1500 years compared to (age depth) modeled luminescence ages after fading corrections. This is observed for the first time in the lake sediments of High Himalayan Crystalline zone. A combination of lipid n-alkane data, Raman spectra and geochemical proxies suggested that this was due to ancient organic carbon (OCancient) that is a mixture of pre-aged (OCpre-aged) and petrogenic (OCpetro) organic carbon within older glacial moraine debris that served as sediment source to the lake. Raman spectra suggest the presence of moderate to highly graphitized OCpetro in all the profile samples. The OCpetro contributed 0.064 ± 0.032% to the sediment and the lake stored 2.5 ± 0.7 Gg OCpetro at variable rates during the last 16 kyr, with the mean burial flux 160 kg OCpetro yr−1. This study implies (1) employing another independent dating method in addition to radiocarbon method using bulk sediment organic matter, if the carbon content is low, to observe any discrepancy, and (2) a need to investigate on the fate of OCpetro as many such small lakes become relict in this region.

Background: Duchenne muscular dystrophy (DMD) is caused by DMD gene mutations. Delandistrogene moxeparvovec is an investigational gene transfer therapy, developed to address the underlying cause of DMD. We report findings from Part 1 (52 weeks) of the two-part EMBARK trial (NCT05096221). Methods: Key inclusion criteria: Ambulatory patients aged ≥4-<8 years with a confirmed DMD mutation within exons 18–79 (inclusive); North Star Ambulatory Assessment (NSAA) score >16 and <29 at screening. Eligible patients were randomized 1:1 to intravenous delandistrogene moxeparvovec (1.33×1014 vg/kg) or placebo. The primary endpoint was change from baseline in NSAA total score to Week 52. Results: At Week 52 (n=125), the primary endpoint did not reach statistical significance, although there was a nominal difference in change from baseline in NSAA total score in the delandistrogene moxeparvovec (2.6, n=63) versus placebo groups (1.9, n=61). Key secondary endpoints (time to rise, micro-dystrophin expression, 10-meter walk/run) demonstrated treatment benefit in both age groups (4-5 and 6-7 years; p<0.05).There were no new safety signals, reinforcing the favorable and manageable safety profile observed to date. Conclusions: Based on the totality of functional assessments including the timed function tests, treatment with delandistrogene moxeparvovec indicates beneficial modification of disease trajectory.

Background: Stroke incidence is rising among younger adults (≤65yrs). Modifiable and behavioural risk factors are linked to stroke; however, limited understanding of knowledge and behaviour exists around preventative/lifestyle medicine (LSM) among this patient population. Study aim was to assess younger adult stroke patients’ lifestyle knowledge, habits, and barriers. Methods: A cross-sectional design was employed. Data were collected through an online, self-reported survey following a routine stroke prevention clinic visit and analyzed using descriptive and inferential statistics. Results: Sample included 103 participants (56.3% women, 60% white, mean age 47.6, 54.5% prior stroke). Majority (63%) understood current healthy lifestyle recommendations around blood pressure, sleep, and alcohol use, but fewer (<24.3%) around exercise and diet. Almost 70% ate processed food weekly, with emotions and social/family situations influencing eating habits. Interestingly, despite not understanding the current recommendations, >80% exercised moderately (3.5d/wk) with work and family responsibilities as main barriers. Over 50% slept <7hrs/night, had moderate to high stress levels, and implemented different coping strategies (food, TV, video games, and exercise). Majority (82.4%) reported willingness to change habits. Conclusions: Our findings provide valuable insight on young adult stroke patients’ preventative/LSM-related knowledge, habits, and barriers and provide new opportunities for the development of brain care-related initiatives.

Changing practice patterns caused by the pandemic have created an urgent need for guidance in prescribing stimulants using telepsychiatry for attention-deficit hyperactivity disorder (ADHD). A notable spike in the prescribing of stimulants accompanied the suspension of the Ryan Haight Act, allowing the prescribing of stimulants without a face-to-face meeting. Competing forces both for and against prescribing ADHD stimulants by telepsychiatry have emerged, requiring guidelines to balance these factors. On the one hand, factors weighing in favor of increasing the availability of treatment for ADHD via telepsychiatry include enhanced access to care, reduction in the large number of untreated cases, and prevention of the known adverse outcomes of untreated ADHD. On the other hand, factors in favor of limiting telepsychiatry for ADHD include mitigating the possibility of exploiting telepsychiatry for profit or for misuse, abuse, and diversion of stimulants. This Expert Consensus Group has developed numerous specific guidelines and advocates for some flexibility in allowing telepsychiatry evaluations and treatment without an in-person evaluation to continue. These guidelines also recognize the need to give greater scrutiny to certain subpopulations, such as young adults without a prior diagnosis or treatment of ADHD who request immediate-release stimulants, which should increase the suspicion of possible medication diversion, misuse, or abuse. In such cases, nonstimulants, controlled-release stimulants, or psychosocial interventions should be prioritized. We encourage the use of outside informants to support the history, the use of rating scales, and having access to a hybrid model of both in-person and remote treatment.

Low phosphorus use efficiency (PUE) is one of the abiotic factors that hamper yield and production potential in chickpea (Cicer arietinum L.). Higher yield coupled with improved PUE can make this crop more adaptive and competitive to wide cropland area, especially on marginal soils having low-level phosphorus (P). To identify chickpea germplasm lines that assimilate phosphorus more efficiently under P-deficient soils, 288 diverse genotypes of chickpea belonging to reference set were evaluated for yield component traits and PUE under field conditions for two consecutive years at two phosphorus levels (low P – no phosphorus application and high P – phosphorus application at 40 kg/ha). Based on 2-year evaluation of data under high and low P soil conditions, we identified strong correlations for traits like number of primary and secondary branches, number of pods, biological yield and seed yield indicating that these traits can be used as proxy traits for PUE. ICC 6571 was the best performing genotype under low P conditions while ICC 6579 yielded maximum under high P regime. We report 16 genotypes namely ICC 1052, ICC 1083, ICC 1098, ICC 1161, ICC 2072, ICC 4418, ICC 4567, ICC 4991, ICC 5504, ICC 5639, ICC 7413, ICC 8350, ICC 9590, ICC 9702, ICC 11584 and ICC 13357 as phosphorus use efficient genotypes based on their better performance for yield and yield-contributing traits under low P compared to high P conditions. These genotypes can be exploited in future as potential donors for development of phosphorus use efficient chickpea cultivars.

In vitro released products of adult Setaria cervi females, microfilariae and extracts showed considerable amounts of collagenase activity. On the basis of per mg protein released in vitro, the products of both microfilariae and adult females exhibited comparable activity but this was much higher than that of extract of microfilariae and adult females. Two collagenase enzymes with molecular masses of 50 kDa and 70 kDa were separated using DEAE-sepharose CL6B and Sephadex G-100 column chromatography. The 50 kDa and 70 kDa collagenase exhibited pH optima of 5.2 and 7.0, respectivly. Considering specific activity, the 50 kDa enzyme was found to contribute about ten times more collagenase activity as compared to the 70 kDa enzyme. An inhibition study revealed obvious differences between them. Thiol group inhibitors such as N-ethylmaleimide and leupeptin inhibited the 50 kDa enzyme but this was strongly activated by dithiothreitol, a thiol group stabilizer. Alternatively, the 70 kDa enzyme showed a sensitivity to a metal chelator and a serine group inhibitor indicating its metalloserine protease nature. The antifilarial drug diethylcarbamazine did not demonstrate any inhibition under in vitro conditions. Both enzymes were significantly inhibited by antibody IgG separated from Wuchereria bancrofti infected human sera, showing a possible immunoprotective role.