Ninety-nine individual plants of a segregating F2 population of a wheat single cross Wen 6×CP18.104.22.168.1 were used in this study to find out whether CP22.214.171.124.1, a derivative of Triticum spelta var. album, carries a novel stripe rust resistance gene other than Yr5. Using molecular marker technology, it was found that only one out of 150 SSR primers could produce amplified polymorphism, and a marker, Xgwm155-147bp, located on chromosome 3A, was found to be linked to the stripe rust resistance gene identified with a genetic distance of 40.5 cM, indicating that the latter was also located on chromosome 3A. Since the only Yr gene previously reported to originate from T. spelta var. album is Yr5, located on 2BL, the only logical inference is that there might be other Yr gene (genes) in T. spelta, and the Yr gene identified in the present study might be a novel one. It was temporarily designated YrSp.