MicroRNAs (miRNAs) are endogenous, non-coding RNAs, which are functional in a variety of biological processes through post-transcriptional regulation of gene expression. However, the role of miRNAs in the interaction between Bacillus thuringiensis and insects remains unclear. In this study, small RNA libraries were constructed for B. thuringiensis-infected (Bt) and uninfected (CK) Spodoptera exigua larvae (treated with double-distilled water) using Illumina sequencing. Utilising the miRDeep2 and Randfold, a total of 233 known and 726 novel miRNAs were identified, among which 16 up-regulated and 34 down-regulated differentially expressed (DE) miRNAs were identified compared to the CK. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that potential target genes of DE miRNAs were associated with ABC transporters, fatty acid metabolism and MAPK signalling pathway which are related to the development, reproduction and immunity. Moreover, two miRNA core genes, SeDicer1 and SeAgo1 were identified. The phylogenetic tree showed that lepidopteran Dicer1 clustered into one branch, with SeDicer1 in the position closest to Spodoptera litura Dicer1. A similar phylogenetic relationship was observed in the Ago1 protein. Expression of SeDicer1 increased at 72 h post infection (hpi) with B. thuringiensis; however, expression of SeDicer1 and SeAgo1 decreased at 96 hpi. The RNAi results showed that the knockdown of SeDicer1 directly caused the down-regulation of miRNAs and promoted the mortality of S. exigua infected by B. thuringiensis GS57. In conclusion, our study is crucial to understand the relationship between miRNAs and various biological processes caused by B. thuringiensis infection, and develop an integrated pest management strategy for S. exigua via miRNAs.

Despite its transformative impact, a systematic approach to Smart PSS development remains elusive. Addressing this, the study introduces a dynamic conceptual model named DHSmart and its accompanying canvas, adaptable to various contexts and technological advancements. Notably, it offers a structured approach to designing ‘Smart’ in Smart PSS, capturing the interplay between data, humans, and smart systems while directing digitalisation that achieves competitive advantage. It also serves as a unifying framework, enabling meaningful interdisciplinary contributions in theory and practice.

Artificial intelligence and cognitive science are two core research areas in design. Artificial intelligence shows the capability of analysing massive amounts of data which supports making predictions, uncovering patterns and generating insights in varying design activities, while cognitive science provides the advantage of revealing the inherent mental processes and mechanisms of humans in design. Both artificial intelligence and cognitive science in design research are focused on delivering more innovative and efficient design outcomes and processes. Therefore, this thematic collection on “Applications of Artificial Intelligence and Cognitive Science in Design” brings together state-of-the-art research in artificial intelligence and cognitive science to showcase the emerging trend of applying artificial intelligence techniques and neurophysiological and biometric measures in design research. Three promising future research directions: 1) human-in-the-loop AI for design, 2) multimodal measures for design, and 3) AI for design cognitive data analysis and interpretation, are suggested by analysing the research papers collected. A framework for integration of artificial intelligence and cognitive science in design, incorporating the three research directions, is proposed to inspire and guide design researchers in exploring human-centred design methods, strategies, solutions, tools and systems.

Todorokite is a common Mn oxide (with a tunnel structure) in the Earth surface environment, and can be obtained by hydrothermal treatment or refluxing process from precursor buserite with a layered structure. Several chemical reaction conditions for the phase transformation from Na-buserite to todorokite at atmospheric pressure were investigated, including temperature, pH, crystallinity of precursor Na-buserite, the amount of the interlayer Mg2+ of the Mg-buserite and clay minerals. The results showed that the conversion rate and crystallinity of todorokite decreased with falling temperature, and Mg-buserite could not be completely transformed to todorokite at lower temperatures (40°C). The poorly crystalline Na-buserite could be converted into todorokite more easily than highly crystalline Na-buserite. Todorokite can be prepared at pH 5–9, but the rate of conversion and crystallinity of todorokite did vary with pH in the order: neutral ≈ alkali > acidic. The conversion rate of todorokite decreased with decreasing interlayer Mg2+ content of the Mg-buserite. The presence of montmorillonite or goethite slowed the formation reaction of todorokite in the refluxing process, and the reaction time was prolonged when the amounts of those minerals were increased.

The early-stage engineering design activities include conceptualising, identifying, and solving an engineering design problem. These activities are essential and standard roles of a design engineer. However, they seem to lack comprehensive practice within the engineering design community. In this study, semi-structured interviews conducted with 18 participants having engineering design backgrounds are presented. The aim of the interviews is to investigate the awareness and practice of the early stage engineering design activities. The participants interviewed practice in countries including France, the United Kingdom, and the United States. Contrary to standard expectations, the results of the interviews show that the early-stage engineering design activities are not comprehensively practised. The results suggest that design engineers' crucial role in identifying unknown problems lacks practice. Also, the data from the interviews provide empirical evidence on the determinants for the lack of comprehensive practice of early-stage engineering design activities. Recommendations on possible interventions to support the practice are presented to expedite innovations and inventions.

The availability of digital data in the fourth industrial revolution brings different trends with new opportunities and challenges for the engineering design community. As an opportunity, these trends would impact engineering design. However, the challenge is finding applications for these trends in engineering design. Crowdsourcing is one of the trends inspired by digital data. It is outsourcing an individually performed task to be mass-performed. This paper explores the application of crowdsourcing in identifying engineering design problems. Identifying an engineering design problem is an aspect of engineering design considered challenging but necessary for inventions. Secondary data from 63 invention-related cases and an interview with a renowned UK inventor are presented. The data contains scenarios on how the engineering design problems solved to qualify for a UK patent grant or application are identified. Lessons from the case studies are presented and discussed, especially regarding crowdsourcing engineering design problems. These seem to be promising ways of supporting the identification of new engineering design problems with inventive benefits once solved.

Crowdfunding is becoming increasingly popular for funding projects, particularly in the domain of product design, by asking a large group of people. Previous studies have indicated that creativity plays a significant role in product design and is considered an important factor of success for new product design and development. However, these studies have not explicitly explored the role of creativity in crowdfunding product design projects. This paper investigates this issue by conducting a case study employing expert evaluations of selected successful and unsuccessful crowdfunding product design project samples. The results of the study show there is a positive relationship between the creativity of a product and the success of its crowdfunding campaign. Therefore, creativity can be considered a success factor of crowdfunding. The study also suggests creative products, especially useful ones, might have more potential to attract people's willingness to fund them. This paper has contributed to the research on design, creativity, product design and development, and funding business models. Most importantly, this paper has raised the significance of creativity in design and business.

The ideation process has a significant impact on the initial concept generation and final product creativity of the design. Visual stimuli play an important role in the process of innovative product design. With the increase in computing capability, generative design methods are widely implemented. In this paper, features of design targets and combinational objects in 2 combinational design tasks are fused using adversarial neural generative networks to form the generated stimuli. It is also used with combinational object pictures to investigate the impact on creativity in design ideation. The study invited designers to use and subjectively self-evaluate the two stimuli in a design task. Through analysis of participant data (n=20), the results showed that the generative stimuli had an advantage over the combinational image stimuli in terms of the smoothness of creativity in the design ideation of outcomes. And there is a positive correlation between designers' years of design education and their tendency to prefer generative stimuli. Based on the results obtained, ideas are provided for the study of the influence of visual and generative stimuli on the designer's ideation process.

Understanding user perceptions of interacting with the virtual world is one of the research focuses in recent years, given the rapid proliferation of virtual reality (VR) and driven to establish the metaverse. Users can generate a familiar connection between their bodies and the virtual world by being embodied in virtual hands, and hand representations can induce users’ embodiment in VR. The sense of embodiment represents the cognitive awareness of one's manifestation and includes three subcomponents: the sense of body ownership, agency and self-location. There is insufficient evidence in the literature about the effects of hand designs on the embodiment, especially based on studying its three subcomponents. This study investigates how virtual hand designs with five realism levels influence the three subcomponents of embodiment in VR. This research employs a self-report questionnaire commonly used in the literature to assess embodiment and evaluates agency and self-location by introducing implicit methods (intentional binding and proprioceptive measurement) derived from psychology. Besides, the objective data of eye tracking is used to explore the connection between embodiment and hand designs, and classifying participants’ eye tracking data to help analyze the link between embodiment and user attention. Overall, this research makes a major contribution through a systematic exploration of users’ embodied experience in VR and offers important evidence of the effects of virtual hand designs on body ownership, agency, and self-location, respectively. In addition, this study provides a valuable reference for further investigation of embodiment through implicit and objective methods, and practical design recommendations for virtual hand design in VR applications.

Computer-aided design (CAD) plays an essential role in creative idea generation on 2D screens during the design process. In most CAD scenarios, virtual object translation is an essential operation, and it is commonly used when designers simulate their innovative solutions. The degrees of freedom (DoF) of virtual object translation modes have been found to directly impact users’ task performance and psychological aspects in simulated environments. Little is known in the existing literature about the sense of agency (SoA), which is a critical psychological aspect emphasizing the feeling of control, in translation modes on 2D screens during the design process. Hence, this study aims to assess users’ SoA in virtual object translation modes on mouse-based, touch-based, and handheld augmented reality (AR) interfaces through subjective and objective measures, such as self-report, task performance, and electroencephalogram (EEG) data. Based on our findings in this study, users perceived a greater feeling of control in 1DoF translation mode, which may help them come up with more creative ideas, than in 3DoF translation mode in the design process; additionally, the handheld AR interface offers less control feel, which may have a negative impact on design quality and creativity, as compared with mouse- and touch-based interfaces. This research contributes to the current literature by analyzing the association between virtual object translation modes and SoA, as well as the relationship between different 2D interfaces and SoA in CAD. As a result of these findings, we propose several design considerations for virtual object translation on 2D screens, which may enable designers to perceive a desirable feeling of control during the design process.

Meat quality is not only influenced by breed but also rearing environment. The aim of this study was to evaluate the influence of different housing environments on growth performance, carcase traits, meat quality, physiological response pre-slaughter and fatty acid composition in two pig breeds. A total of 120 growing pigs at 60-70 days of age were arranged in a 2 × 2 factorial design with the breeds (Duroc × Landrace × Large White [D × L × LW] and Duroc × Landrace × Min pig [D × L × M]) and environmental enrichment (barren concrete floor or enriched with straw bedding) as factors. Each treatment was performed in triplicate with ten pigs per replicate. The pigs housed in the enriched environment exhibited a higher average daily gain, average daily feed intake, saturated fatty acid percentage and backfat depth than the pigs reared in the barren environment. Plasma cortisol levels were lower and growth hormone higher in enriched compared to barren pens. The D × L × M pigs showed lower cooking loss compared with the D × L × LW pigs. Moreover, the D × L × M pigs exhibited poor growth performance but had a better water-holding capacity. Only carcase traits and meat quality interaction effects were observed. We concluded that an enriched environment can reduce preslaughter stress and improve the growth performance of pigs and modulate the fatty acid composition of pork products.

Nicotine 2,6-dihydroxybenzoate is a nicotine salt that can be used as the nicotine source in tobacco products. X-ray powder diffraction data, unit-cell parameters, and space group for nicotine 2,6-dihydroxybenzoate, C10H15N2⋅C7H5O4, are reported [a = 7.726(8) Å, b = 11.724(3) Å, c = 9.437(1) Å, α = 90°, β = 109.081(3)°, γ = 90°, unit-cell volume V = 802.902 Å3, Z = 2, ρcal = 1.309 g cm−3, and space group P21] at room temperature. All measured lines were indexed and were consistent with the P21 space group.

Climbing robots that use bionic claws can climb vertical or even inverted rough surfaces. However, wall-climbing robots with unidirectional spiny feet cannot crawl horizontally or downward on vertical rough surfaces. In this paper, a pair of gripping spiny feet is used to give a robot the capacity to crawl in any direction on a rough wall. On the basis of observations of the gecko’s method for grasping onto a vertical rough surface, a multilevel interlocking structure is proposed. A spherical contact model of the claw tip on a vertical rough surface is established, and the influences of the contact angle, friction coefficient, and other factors on the grappling claw action are analyzed. Moreover, the optimal structure of the grappling claws is proposed. The force during the grasping and detachment of the mechanism and the influence of the number of feet on grasping performance are determined through experiments. Furthermore, a six-legged wall-climbing robot is designed and evaluated in terms of crawling on a vertical rough surface at various angles. The feasibility of using an opposed gripping mechanism to allow a robot to crawl in any direction on a vertical rough surface is also verified.

Angiopoietin-like 4 (ANGPTL4) is a potent regulator of TAG metabolism, but knowledge of the mechanisms underlying ANGPTL4 transcription in response to fatty acids is still limited in teleost. In the current study, we explored the molecular characterisation of ANGPTL4 and regulatory mechanisms of ANGPTL4 in response to fatty acids in large yellow croaker (Larimichthys crocea). Here, croaker angptl4 contained a 1416 bp open reading frame encoding a protein of 471 amino acids with highly conserved 12-amino acid consensus motif. Angptl4 was widely expressed in croaker, with the highest expression in the liver. In vitro, oleic and palmitic acids (OA and PA) treatments strongly increased angptl4 mRNA expression in croaker hepatocytes. Moreover, angptl4 expression was positively regulated by PPAR family (PPAR-α, β and γ), and expression of PPARγ was also significantly increased in response to OA and PA. Moreover, inhibition of PPARγ abrogated OA- or PA-induced angptl4 mRNA expression. Beyond that, PA might increase angptl4 expression partly via the insulin signalling. Overall, the expression of ANGPTL4 is strongly upregulated by OA and PA via PPARγ in the liver of croaker, which contributes to improve the understanding of the regulatory mechanisms of ANGPTL4 in fish.

Almost all hospitals are equipped with air-conditioning systems to provide a comfortable environment for patients and staff. However, the accumulation of dust and moisture within these systems increases the risk of transmission of microbes and have on occasion been associated with outbreaks of infection. Nevertheless, the impact of air-conditioning on the transmission of microorganisms leading to infection remains largely uncertain. We conducted a scoping review to screen systematically the evidence for such an association in the face of the coronavirus disease 2019 epidemic. PubMed, Embase and Web of Science databases were explored for relevant studies addressing microbial contamination of the air, their transmission and association with infectious diseases. The review process yielded 21 publications, 17 of which were cross-sectional studies, three were cohort studies and one case−control study. Our analysis showed that, compared with naturally ventilated areas, microbial loads were significantly lower in air-conditioned areas, but the incidence of infections increased if not properly managed. The use of high-efficiency particulate air (HEPA) filtration not only decreased transmission of airborne bioaerosols and various microorganisms, but also reduced the risk of infections. By contrast, contaminated air-conditioning systems in hospital rooms were associated with a higher risk of patient infection. Cleaning and maintenance of such systems to recommended standards should be performed regularly and where appropriate, the installation of HEPA filters can effectively mitigate microbial contamination in the public areas of hospitals.