This study was carried out on some epidemiological aspects and vector role of ticks infesting layers in Faisalabad (Pakistan). The prevalence of tick infestation was found as 11% (88/800), 24% (192/800) and 12.7% (413/3250) on Indigenous, Babcock and White Leghorn varieties of layers, respectively. Two species of ticks namely Argas persicus (10%; 485/4,850) and Argas vespertilionis (3.5%; 170/4,850) were recorded. Tick infestation was found higherin summermonths, in youngerbirds and in areas having densely located farms. The vector role of Argas persicus and Argas vespertilionis in the transmission of spirochaetosis in layer chicken was confirmed through isolation of Borrelia anserina from naturally infected ticks and their experimental transmission. Three species of bacteria namely Staphylococcus aureus, Salmonella pullorum and Escherichia coli were isolated from the triturated Argas persicus and Argas vespertilionis. Results of the present study were not different from those reported in literature, but for the first time in the study area. These results warrant for further research and also following effective tick control programs in orderto save the farmers from economic losses.