Tactical skills in sport are the basis for current models of invasion team sports instruction, and relate to other psychological variables that exert a mediating influence on people’s normal physical activity. This study aimed to validate the Spanish adaptation of the Tactical Skills Inventory for Sports by Elferink-Gemser, Visscher, Richart, and Lemmink (2004) and verify its factorial invariance. The sample included 540 participants 12.89 ± 1.73 years of age (10 to 17 years old; 333 males and 207 females). The adaptation and translation of the original questionnaire followed the methodological steps established by the International Test Commission. Then two measurement models with the original questionnaire’s four-factor structure were compared. Confirmatory analyses were conducted using the software AMOS 21 and maximum likelihood estimation. Results indicated a four-factor structure (positioning and deciding, knowing about ball actions, knowing about others, acting in changing situations) was viable and showed goodness of fit to the data, with adequate indices of reliability (α between .72 and .86) and validity (GFI = .955; RMSEA = .044; CFI = .974), and strong evidence of stability in the factor structure. Moreover, factorial invariance was observed between participants who do and do not practice team sports, with practitioners scoring higher than non-practitioners on all factors (p < .001; effect sizes between .97 and 1.08).