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Chapter 16 - Cryopreservation of Sperm for IVF

Semen Samples and Individual Sperm

from Section 3 - Gametes

Published online by Cambridge University Press:  11 May 2017

By
Nina Desai  and
Pooja Rambhia
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Fertility Associates, New Zealand
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Information
Principles of IVF Laboratory Practice
Optimizing Performance and Outcomes
 , pp. 112 - 118
Publisher: Cambridge University Press
Print publication year: 2017

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References

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Isachenko, V., Maettner, R., Petrunkina, A. M., Sterzik, K., Mallmann, P. et al. Vitrification of human ICSI/IVF spermatozoa without cryoprotectants: new capillary technology. J Androl 2012; 33:462–8.CrossRefGoogle ScholarPubMed
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World Health Organization (WHO). laboratory Manual for the Examination and Processing of Human Semen (5th edn.) Geneva: World Health Organization, 2010.Google Scholar
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Gangrade, B. K. Cryopreservation of testicular and epididymal sperm: techniques and clinical outcomes of assisted conception. Clinics 2013; 68(Suppl 1):131–40.CrossRefGoogle ScholarPubMed
Cohen, J., Garrisi, G. J., Congedo-Ferrara, T. A., Kieck, K. A., Schimmel, T. W. et al. Cryopreservation of single human spermatozoa. Hum Reprod 1997; 12:994–1001.CrossRefGoogle ScholarPubMed
Desai., N. N., Blackmon, H. and Goldfarb, J. Single sperm cryopreservation on cryoloops: an alternative to hamster zona for freezing individual spermatozoa. Reprod Biomed Online 2004; 9:47–53.CrossRefGoogle ScholarPubMed
Desai, N., Goldberg, J., Austin, C., Sabanegh, E. and Falcone, T. Cryopreservation of individually selected sperm: methodology and case report of a clinical pregnancy. J Assist Reprod & Genetics 2012; 29:375–9.CrossRefGoogle ScholarPubMed
Coetzee, K., Ozgur, K., Berkkanoglu, M., Bulut, H. and Isikli, A. Reliable single sperm cryopreservation in Cell Sleepers for azoospermia management. Andrologia 2015.CrossRefGoogle Scholar

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