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  • Print publication year: 2017
  • Online publication date: May 2017

Chapter 16 - Cryopreservation of Sperm for IVF

from Section 3 - Gametes
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2.Bunge, R. G. and Sherman, J. K. Fertilizing capacity of frozen human spermatozoa. Nature 1953; 172:767–8.
3.Di Santo, M., Tarozzi, N., Nadalini, M. and Borini, A. Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. Adv Urol 2012; 2012:854837.
4.Isachenko, V., Isachenko, E., Katkov, I. I., Montag, M., Dessole, S. et al. Cryoprotectant-free cryopreservation of human spermatozoa by vitrification and freezing in vapor: effect on motility, DNA integrity, and fertilization ability. Biol Reprod 2004; 71:1167–73.
5.Isachenko, V., Maettner, R., Petrunkina, A. M., Sterzik, K., Mallmann, P. et al. Vitrification of human ICSI/IVF spermatozoa without cryoprotectants: new capillary technology. J Androl 2012; 33:462–8.
6.Slabbert, M., du Plessis, S. S. and Huyser, C. Large volume cryoprotectant-free vitrification: an alternative to conventional cryopreservation for human spermatozoa. Andrologia 2015; 47:594–9.
7.World Health Organization (WHO). laboratory Manual for the Examination and Processing of Human Semen (5th edn.) Geneva: World Health Organization, 2010.
8.AbdelHafez, F., Bedaiwy, M., El-Nashar, S. A., Sabanegh, E. and Desai, N. Techniques for cryopreservation of individual or small numbers of human spermatozoa: a systematic review. Hum Reprod Update 2009; 15:153–64.
9.Gangrade, B. K. Cryopreservation of testicular and epididymal sperm: techniques and clinical outcomes of assisted conception. Clinics 2013; 68(Suppl 1):131–40.
10.Cohen, J., Garrisi, G. J., Congedo-Ferrara, T. A., Kieck, K. A., Schimmel, T. W. et al. Cryopreservation of single human spermatozoa. Hum Reprod 1997; 12:9941001.
11.Desai., N. N., Blackmon, H. and Goldfarb, J. Single sperm cryopreservation on cryoloops: an alternative to hamster zona for freezing individual spermatozoa. Reprod Biomed Online 2004; 9:4753.
12.Desai, N., Goldberg, J., Austin, C., Sabanegh, E. and Falcone, T. Cryopreservation of individually selected sperm: methodology and case report of a clinical pregnancy. J Assist Reprod & Genetics 2012; 29:375–9.
13.Coetzee, K., Ozgur, K., Berkkanoglu, M., Bulut, H. and Isikli, A. Reliable single sperm cryopreservation in Cell Sleepers for azoospermia management. Andrologia 2015.