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Chapter 6 - ICSI for IVF

Training Protocol

from Section 1 - Starting a New Laboratory and Training Protocols

Published online by Cambridge University Press:  07 August 2023

By
Cristina Hickman
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Intracytoplasmic sperm injection (ICSI) is an insemination procedure that allows for a single sperm to be inserted directly into the ooplasm using a needle, therefore bypassing issues involving sperm that may be unable to reach an oocyte and/or bind to the zona pellucida. ICSI is traditionally indicated for male-factor infertility and/or following failed or low fertilization with conventional in vitro fertilization (IVF) in a preceding cycle. Because it is a technically demanding procedure, a structured and closely monitored training programme is required to achieve optimal success rates with ICSI. The objective of this chapter is to outline an ICSI training protocol by which the technical skills are acquired without compromising clinical outcome.

Keywords

sperm injection, ICSI, embryologist, training, micromanipulation
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 38 - 46
Publisher: Cambridge University Press
Print publication year: 2023

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References

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