Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-77c89778f8-m8s7h Total loading time: 0 Render date: 2024-07-19T23:36:03.772Z Has data issue: false hasContentIssue false

Chapter 6 - ICSI for IVF

Training Protocol

from Section 1 - Starting a New Laboratory and Training Protocols

Published online by Cambridge University Press:  07 August 2023

By
Cristina Hickman
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
Get access

Summary

Intracytoplasmic sperm injection (ICSI) is an insemination procedure that allows for a single sperm to be inserted directly into the ooplasm using a needle, therefore bypassing issues involving sperm that may be unable to reach an oocyte and/or bind to the zona pellucida. ICSI is traditionally indicated for male-factor infertility and/or following failed or low fertilization with conventional in vitro fertilization (IVF) in a preceding cycle. Because it is a technically demanding procedure, a structured and closely monitored training programme is required to achieve optimal success rates with ICSI. The objective of this chapter is to outline an ICSI training protocol by which the technical skills are acquired without compromising clinical outcome.

Keywords

sperm injection, ICSI, embryologist, training, micromanipulation
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 38 - 46
Publisher: Cambridge University Press
Print publication year: 2023

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Katz, E., Watts, L. D., Wright, K. E., et al. Effect of incremental time experience on the results of in vitro fertilisation with intracytoplasmic sperm injection (ICSI). J Assist Reprod Genet 1996; 13 (6):501–4.CrossRefGoogle ScholarPubMed
Ebner, T., Yaman, C., Moser, M., et al. A prospective study on oocyte survival rate after ICSI: influence of injection technique and morphological features. J Assist Reprod Genet 2001; 18(12):623–8.Google Scholar
Daniel, C. E., Hickman, C., Wilkinson, T., et al. Maximising success rates by improving ICSI technique: which factors affect outcome? Fertil Steril 2015; 104(3):e95–6.Google Scholar
Dumoulin, J. M., Coonen, E., Bras, M., et al. Embryo development and chromosomal anomalies after ICSI: effect of the injection procedure. Hum Reprod 2001; 16(2):306–12.CrossRefGoogle ScholarPubMed
Tsai, M. Y., Huang, F. J., Kung, F. T., et al. Influence of polyvinylpyrrolidone on the outcome of intracytoplasmic sperm injection. J Reprod Med 2000; 45(2):115–20.Google ScholarPubMed
Nagy, Z. P., Liu, J., Joris, H., et al. The influence of the site of sperm deposition and mode of oolemma breakage at intracytoplasmic sperm injection on fertilization and embryo development rates. Hum Reprod 1995; 10 (12):3171–7.Google Scholar
Rubino, P., Viganò, P., Luddi, A. and Piomboni, P. The ICSI procedure from past to future: a systematic review of the more controversial aspects. Hum Reprod Update 2016; 22(2):194–227.Google ScholarPubMed
Flaherty, S. P., Payne, D., Swann, N. J. and Mattews, C. D. Aetiology of failed and abnormal fertilization after intracytoplasmic sperm injection. Hum Reprod 1995; 10(10):2623–9.CrossRefGoogle ScholarPubMed
Blake, M., Garrisi, J., Tomkin, G. and Cohen, J. Sperm deposition site during ICSI affects fertilization and development. Fertil Steril 2000; 73(1):31–7.CrossRefGoogle ScholarPubMed
Nagy, Z. P., Oliveira, S. A., Abdelmassih, V. and Abdelmassih, R. Novel use of laser to assist ICSI for patients with fragile oocytes: a case report. Reprod Biomed Online 2002; 4(1):27–31.CrossRefGoogle ScholarPubMed

Save book to Kindle

To save this book to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×