Hostname: page-component-8448b6f56d-42gr6 Total loading time: 0 Render date: 2024-04-20T04:36:14.926Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic stability of cryopreserved ornamental Lilium germplasm

Published online by Cambridge University Press:  04 May 2022

Jae-young Song Open the ORCID record for Jae-young Song [Opens in a new window] ,
Jung-yoon Yi ,
Jinjoo Bae ,
Jung-ro Lee ,
Mun-sup Yoon  and
Young-yi Lee
Jae-young Song
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
Jung-yoon Yi
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
Jinjoo Bae
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
Jung-ro Lee
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
Mun-sup Yoon
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
Young-yi Lee*
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
*
Author for correspondence: Young-yi Lee, E-mail: youngyi@korea.kr
Get access Rights & Permissions [Opens in a new window]

Abstract

The genus Lilium contains a number of ornamental crop species, which are commercially important in many countries. As they are vegetatively propagated, maintaining genetic stability is essential for their efficient conservation. In this study, we investigated the genetic stability of regenerated plants of three cultivars (L. bolanderi ‘Lenora’, L. bolanderi ‘Mount Duckling’ and L. bolanderi ‘Mount Dragon’) and one variety (L. callosum var. flavum) after cryopreservation, compared with fresh (donor) and non-cryopreserved plants using morphological traits and ISSR markers. No differences in morphological parameters including flower, stigma and pollen colour, floral spots, floral direction or polymorphic bands were observed between control (fresh and non-cryopreserved) and cryopreserved plantlets. In addition, based on the resulting UPGMA dendrogram, the four taxa were divided into different clusters. All cryopreserved, non-cryopreserved and fresh plants in each group could be grouped together in a single cluster with more than 97 or 100% similarity. The results suggest a very low level or the absence of genetic variation in terms of morphological and genetic stability among the plants regenerated after cryopreservation.

Keywords

CryopreservationGenetic stabilityISSR
Type
Short Communication
Information
Plant Genetic Resources , Volume 20 , Issue 1 , February 2022 , pp. 66 - 68
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahuja, MR (2011) Strategies for conservation of germplasm in endemic redwoods in the face of climate change: a review. Plant Genetic Resources 9, 411–422.CrossRefGoogle Scholar
Ahuja, S, Mandal, BB, Dixit, S and Srivastava, PS (2002) Molecular, phenotypic and biosynthetic stability in Dioscorea floribunda plants derived from cryopreserved shoot tips. Plant Science 163, 971–977.CrossRefGoogle Scholar
Bouman, H, Tiekstra, A, Petutschnig, E, Homan, M and Schreurs, R (2003) Cryopreservation of Lilium species and cultivars. Acta Horticulture 612, 147–154.CrossRefGoogle Scholar
Bush, CM, Rollins, D and Smith, GL (2010) The phylogeny of the Southeastern United States Hymenocallis (Amaryllidaceae) based on ISSR fingerprinting and morphological data. Castanea 75, 368–380.CrossRefGoogle Scholar
Chen, XL, Li, JH, Xin, X, Zhang, ZE, Xin, PP and Lu, XX (2011) Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification. South African Journal of Botany 77, 397–403.CrossRefGoogle Scholar
Dhital, SP, Lim, HT and Manandhar, HK (2009) Elimination of potato virus (PLRV and PVY) by cryopreservation of in vitro grown shoot tips of potato (Solanum tuberosum L.). Horticulture. Environment, and Biotechnology 50, 233–239.Google Scholar
Engelmann, F (2014) Cryopreservation of clonal crops: a review of key parameters. Acta Horticulture 1039, 31–39.CrossRefGoogle Scholar
Fki, L, Bouaziz, N, Sahnoun, N, Swennen, R, Drira, N and Panis, B (2011) Palm cryobanking. CryoLetters 32, 451–462.Google ScholarPubMed
Hirai, D, Shirai, K, Shirai, S and Sakai, A (1998) Cryopreservation of in vitro grown meristems of strawberry (Fragaria × ananassa Duch) by encapsulation-vitrification. Euphytica 101, 109–115.CrossRefGoogle Scholar
Khandagale, K, Padmakar, B, Lakshmana Reddy, DC, Sane, A and Aswath, C (2014) Genetic diversity analysis and barcoding in tuberose (Polianthes tuberosa L.) cultivars using RAPD and ISSR markers. Journal of Horticultural Sciences 9, 5–11.Google Scholar
Kim, HH, Lee, JG, Shin, DJ, Ko, HC, Gwag, JG, Cho, EG and Engelmann, F (2009) Development of alternative plant vitrification solutions in droplet-vitrification procedures. CryoLetters 30, 320–334.Google ScholarPubMed
Liu, K and Muse, SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics (Oxford, England) 21, 2128–2129.CrossRefGoogle ScholarPubMed
Liu, X and Yang, G (2012) Adventitious shoot regeneration of oriental lily (Lilium orientalis) and genetic stability evaluation based on ISSR marker variation. In Vitro Cellular and Developmental Biology – Plant 48, 172–179.CrossRefGoogle Scholar
Raji, MR, Lotfi, M, Tohidfar, M, Zahedi, B, Carra, A, Abbate, L and Carimi, F (2018) Somatic embryogenesis of muskmelon (Cucumis melo L.) and genetic stability assessment of regenerants using flow cytometry and ISSR markers. Protoplasma 255, 873–883.CrossRefGoogle ScholarPubMed
Tamura, K, Dudley, J, Nei, M and Kumar, S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.CrossRefGoogle ScholarPubMed
Towill, LE (1988) Genetic considerations for germplasm preservation of clonal materials. HortScience 23, 91–95.Google Scholar
Vidyagina, EO, Kharchenko, NN and Shestibratov, KA (2021) Efficient cryopreservation of Populus tremula by In Vitro-Grown axillary buds and genetic stability of recovered plants. Plants 10, 77.CrossRefGoogle ScholarPubMed
Wang, YL, Sun, GL, Li, SF, Qian, ZQ and Zhao, GF (2008) Population structure and genetic diversity of Clintonia udensis Trautv. Et Mey. (Liliaceae). International Journal of Plant Sciences 169, 1238–1247.CrossRefGoogle Scholar
Yadav, R, Yadav, N, Pal, M and Goutam, U (2013) Multiple shoot proliferation, bulblet induction and evaluation of genetic stability in Asiatic hybrid lily (Lilium sp.). Indian Journal of Plant Physiology 18, 354–359.CrossRefGoogle Scholar
Yi, JY, Lee, GA, Chung, JW, Lee, SY and Lim, KB (2013) Efficient cryopreservation of Lilium spp. shoot tips using droplet-vitrification. Plant Breeding and Biotechnology 1, 131–136.CrossRefGoogle Scholar
Yin, ZF, Zhao, B, Bi, WL, Chen, L and Wang, QC (2013) Direct shoot regeneration from basal leaf segments of Lilium and assessment of genetic stability in regenerants by ISSR and AFLP markers. In Vitro Cellular and Developmental Biology – Plant 49, 333–342.CrossRefGoogle Scholar
Yin, ZF, Bi, WL, Chen, L, Zhao, B, Volk, GM and Wang, QC (2014) An efficient, widely applicable cryopreservation of Lilium shoot tips by droplet vitrification. Acta Physiologiae Plantarum 36, 1683–1692.CrossRefGoogle Scholar
Supplementary material: File

Song et al. supplementary material

Tables S1-S3

Download Song et al. supplementary material(File)
File 43.5 KB