Skip to main content Accessibility help

Expression changes in the TaNAC2 and TaNAC69-1 transcription factors in drought stress tolerant and susceptible accessions of Triticum boeoticum

  • Maryam Nazari (a1), Kiarash Jamshidi Goharrizi (a2), Sayyed Saeed Moosavi (a1) and Mahmood Maleki (a3)


Triticum boeoticum is a valuable gene source for tolerance to drought stress. In order to study the effect of drought stress on this plant, and to understand its adaptive mechanisms at the molecular level, 10 accessions of T. boeoticum were evaluated under non- and drought stress conditions. Evaluation of 31 different phenological, morpho-physiological and root-related traits showed that there were significant differences between accessions. Using the bi-plot resulting from the PCA, the studied traits and accessions were separated in different groups. The most tolerant (B5) and susceptible (B6) accessions to drought stress were identified, so these accessions were used for assessment of changes in the TaNAC2 and TaNAC69-1 transcription factors (TFs) expression. The results showed that in the most tolerant and susceptible accessions, TaNAC2 and TaNAC69-1 expression levels increased between non-stress and stress conditions significantly, but the increased level of these two genes expression in the most tolerant accession was much higher than the most susceptible accession. According to the obtained results, T. boeoticum can be a suitable and promising gene source for improving modern wheat. In addition, the results of TFs expression could improve our understanding about the complex mechanisms associated with drought tolerance in wheat, especially wild wheat.


Corresponding author

*Corresponding author. E-mail:;


Hide All

Maryam Nazari and Kiarash Jamshidi Goharrizi contributed equally to this work as co-first authors.



Hide All
Aida, M, Ishida, T, Fukaki, H, Fujisawa, H and Tasaka, M (1997) Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant. Plant Cell 9: 841857.
Ashraf, M (2010) Inducing drought tolerance in plants: recent advances. Biotechnology Advances 28: 169183.
Blum, A (2009) Effective use of water (EUW) and not water-use efficiency (WUE) is the target of crop yield improvement under drought stress. Field Crops Research 112: 119123.
Budak, H, Kantar, M and Yucebilgili Kurtoglu, K (2013) Drought tolerance in modern and wild wheat. The Scientific World Journal 2013: 548246.
Cochard, H, Coll, L, Le Roux, X and Améglio, T (2002) Unraveling the effects of plant hydraulics on stomatal closure during water stress in walnut. Plant Physiology 128: 282290.
Collinge, M and Boller, T (2001) Differential induction of two potato genes, Stprx2 and StNAC, in response to infection by Phytophthora infestans and to wounding. Plant Molecular Biology 46: 521529.
Dvorak, J, Luo, M and Yang, Z (1998) Genetic evidence on the origin of Triticum aestivum L. In: The origins of agriculture and crop domestication. Proceedings of the Harlan symposium. ICARDA, Aleppo, pp. 235251.
Ernst, HA, Olsen, AN, Skriver, K, Larsen, S and Leggio, LL (2004) Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors. EMBO Reports 5: 297303.
Fujita, M, Fujita, Y, Maruyama, K, Seki, M, Hiratsu, K, Ohme-Takagi, M, Tran, LS, Yamaguchi-Shinozaki, K and Shinozaki, K (2004) A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. The Plant Journal: for Cell and Molecular Biology 39: 863876.
Hu, H, Dai, M, Yao, J, Xiao, B, Li, X, Zhang, Q and Xiong, L (2006) Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proceedings of the National Academy of Sciences of the USA 103: 1298712992.
Hu, H, You, J, Fang, Y, Zhu, X, Qi, Z and Xiong, L (2008) Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice. Plant Molecular Biology 67: 169181.
Jamshidi Goharrizi, K, Wilde, HD, Amirmahani, F, Moemeni, MM, Zaboli, M, Nazari, M, Moosavi, SS and Jamalvandi, M (2018) Selection and validation of reference genes for normalization of qRT-PCR gene expression in wheat (Triticum durum L.) under drought and salt stresses. Journal of Genetics 97: 14331444.
Livak, KJ and Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25: 402408.
Lopes, M, Reynolds, M, Jalal-Kamali, M, Moussa, M, Feltaous, Y, Tahir, I, Barma, N, Vargas, M, Mannes, Y and Baum, M (2012) The yield correlations of selectable physiological traits in a population of advanced spring wheat lines grown in warm and drought environments. Field Crops Research 128: 129136.
Lu, M, Ying, S, Zhang, DF, Shi, YS, Song, YC, Wang, TY and Li, Y (2012) A maize stress-responsive NAC transcription factor, ZmSNAC1, confers enhanced tolerance to dehydration in transgenic Arabidopsis. Plant Cell Reports 31: 17011711.
Mao, X, Zhang, H, Qian, X, Li, A, Zhao, G and Jing, R (2012) TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis. Journal of Experimental Botany 63: 29332946.
Mguis, K, Brahim, NB, Albouchi, A, Yakkoubi-Tej, M, Mahjoub, A and Ouerghi, Z (2008) Phenotypic responses of the wild wheat relative Aegilops geniculata Roth and wheat (Triticum durum Desf.) to experimentally imposed salt stress. Genetic Resources and Crop Evolution 55: 665674.
Mguis, K, Albouchi, A, Abassi, M, Khadhri, A, Ykoubi-Tej, M, Mahjoub, A, Brahim, NB and Ouerghi, Z (2013) Responses of leaf growth and gas exchanges to salt stress during reproductive stage in wild wheat relative Aegilops geniculata Roth. and wheat (Triticum durum Desf.). Acta Physiologiae Plantarum 35: 14531461.
Naghavi, M, Malaki, M, Alizadeh, H, Pirseiedi, M and Mardi, M (2010) An assessment of genetic diversity in wild diploid wheat Triticum boeoticum from west of Iran using RAPD, AFLP and SSR markers. Journal of Agricultural Science and Technology 11: 585598.
Nazari, M, Moosavi, SS and Maleki, M (2018) Morpho-physiological and proteomic responses of Aegilops tauschii to imposed drought stress. Plant Physiology and Biochemistry 132: 445452.
Nuruzzaman, M, Manimekalai, R, Sharoni, AM, Satoh, K, Kondoh, H, Ooka, H and Kikuchi, S (2010) Genome-wide analysis of NAC transcription factor family in rice. Gene 465: 3044.
Pour-Aboughadareh, A, Mahmoudi, M, Moghaddam, M, Ahmadi, J, Mehrabi, AA and Alavikia, SS (2017) Agro-morphological and molecular variability in Triticum boeoticum accessions from Zagros Mountains, Iran. Genetic Resources and Crop Evolution 64: 545556.
Riechmann, JL, Heard, J, Martin, G, Reuber, L, Jiang, C-Z, Keddie, J, Adam, L, Pineda, O, Ratcliffe, O and Samaha, R (2000) Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science 290: 21052110.
Sakuraba, Y, Kim, YS, Han, SH, Lee, BD and Paek, NC (2015) The Arabidopsis transcription factor NAC016 promotes drought stress responses by repressing AREB1 transcription through a trifurcate feed-forward regulatory loop involving NAP. Plant Cell 27: 17711787.
Singh, K, Ghai, M, Garg, M, Chhuneja, P, Kaur, P, Schnurbusch, T, Keller, B and Dhaliwal, HS (2007) An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum x T. monococcum RIL population. Theoretical and Applied Genetics 115: 301312.
Sultan, MARF, Hui, L, Yang, LJ and Xian, ZH (2012) Assessment of drought tolerance of some Triticum l. species through physiological indices. Czech Journal of Genetics and Plant Breeding 48: 178184.
Tabatabaei, SF and Maassoumi, TR (2001) Triticum boeoticum ssp thaoudar ‘exists’ in Iran!. Cereal Research Communications 29: 121126.
Tang, Y, Liu, M, Gao, S, Zhang, Z, Zhao, X, Zhao, C, Zhang, F and Chen, X (2012) Molecular characterization of novel TaNAC genes in wheat and overexpression of TaNAC2a confers drought tolerance in tobacco. Physiologia Plantarum 144: 210224.
Wang, H, Zhao, S, Gao, Y and Yang, J (2017) Characterization of Dof transcription factors and their responses to osmotic stress in poplar (Populus trichocarpa). PLoS ONE 12: e0170210.
Xue, G-P, Bower, NI, McIntyre, CL, Riding, GA, Kazan, K and Shorter, R (2006) TaNAC69 from the NAC superfamily of transcription factors is up-regulated by abiotic stresses in wheat and recognises two consensus DNA-binding sequences. Functional Plant Biology 33: 4357.
Yamaguchi, M, Mitsuda, N, Ohtani, M, Ohme-Takagi, M, Kato, K and Demura, T (2011) Vascular-related NAC-domain 7 directly regulates a broad range of genes for xylem vessel differentiation. BMC Proceedings 5(Suppl 7): O37O37.


Type Description Title
Supplementary materials

Nazari et al. supplementary material
Table S1

 Word (15 KB)
15 KB

Expression changes in the TaNAC2 and TaNAC69-1 transcription factors in drought stress tolerant and susceptible accessions of Triticum boeoticum

  • Maryam Nazari (a1), Kiarash Jamshidi Goharrizi (a2), Sayyed Saeed Moosavi (a1) and Mahmood Maleki (a3)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed