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Delta-aminolevulinate dehydratase activity in red blood cells of rats infected with Trypanosoma evansi

  • RAQUELI T. FRANÇA (a1), ALEKSANDRO S. DA SILVA (a2), PATRÍCIA WOLKMER (a1), VITOR A. OLIVEIRA (a3), MARIA E. PEREIRA (a3), MARTA L. R. LEAL (a4), CÁSSIA B. SILVA (a1), MATHEUS A. G. NUNES (a3), VALDERI L. DRESSLER (a3), CINTHIA M. MAZZANTI (a1), SILVIA G. MONTEIRO (a2) and SONIA T. A. LOPES (a1)...

Summary

The aim of this study was to evaluate the activity of delta-aminolevulinate dehydratase (δ-ALA-D) in red blood cells of rats infected with Trypanosoma evansi and establish its association with haematocrit, serum levels of iron and zinc and lipid peroxidation. Thirty-six male rats (Wistar) were divided into 2 groups with 18 animals each. Group A was non-infected while Group B was intraperitoneally infected, receiving 7·5×106 trypomastigotes per animal. Each group was divided into 3 subgroups of 6 rats and blood was collected during different periods post-infection (p.i.) as follows: day 5 (A1 and B1), day 15 (A2 and B2) and day 30 PI (A3 and B3). Blood samples were collected by cardiac puncture to estimate red blood cell parameters (RBC), δ-ALA-D activity and serum levels of iron, zinc and thiobarbituric acid reactive substances (TBARS). Rats in group B showed a significant (P<0·05) reduction of RBC count, haemoglobin concentration and haematocrit at days 5 and 15 p.i. The activity of δ-ALA-D in blood was significantly (P<0·001) increased at days 15 and 30 p.i. δ-ALA-D activity in blood had a significant (P<0·05) negative correlation with haematocrit (r=−0·61) and haemoglobin (r=−0·70) at day 15 p.i. There was a significant (P<0·05) decrease in serum iron and zinc levels and an increase in TBARS levels (P<0·05) during infection. The δ-ALA-D activity in blood was negatively correlated with the levels of iron (r=−0·68) and zinc (r=−0·57) on day 30 p.i. It was concluded that the increased activity of δ-ALA-D in blood might have occurred in response to the anaemia in remission as heme synthesis was enhanced.

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Corresponding author

*Corresponding author: Departamento de Pequenos Animais, Universidade Federal de Santa Maria, Camobi – 97, Hospital Veterinário, Sala 103. 97105-900, Santa Maria – RS, Brasil. Tel: and Fax: +55 55 32208814. E-mail: raquelifranca@yahoo.com.br

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Keywords

Delta-aminolevulinate dehydratase activity in red blood cells of rats infected with Trypanosoma evansi

  • RAQUELI T. FRANÇA (a1), ALEKSANDRO S. DA SILVA (a2), PATRÍCIA WOLKMER (a1), VITOR A. OLIVEIRA (a3), MARIA E. PEREIRA (a3), MARTA L. R. LEAL (a4), CÁSSIA B. SILVA (a1), MATHEUS A. G. NUNES (a3), VALDERI L. DRESSLER (a3), CINTHIA M. MAZZANTI (a1), SILVIA G. MONTEIRO (a2) and SONIA T. A. LOPES (a1)...

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