Microscopy and Microanalysis: Volume 23 -

Self-Blotting Nanowire Grids for Cryo-EM Sample Preparation
Hui Wei, Venkat Dandey, Zhening Zhang, Ashleigh Raczkowski, Bridget Carragher, Clinton S. Potter
Published online by Cambridge University Press:

04 August 2017, pp. 848-849
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Almost every aspect of cryo electron microscopy (CryoEM) has been automated over the last few decades. One of the challenges that remains to be addressed is the robust and reliable preparation of vitrified specimens of suitable ice thickness. The development of a new self-blotting nanowire (Zhang et al., 2013) grid in conjunction with a piezo electric dispensing robot called Spotiton (Jain et al., 2012) enables spreading a sample to a thin film without the use of externally applied filter paper. This new approach has the advantage of using small amounts of protein material, resulting in large areas of ice of a well- defined thickness containing evenly distributed particles (Razinkov et al., 2016).

Microscopy and Microanalysis