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Soluble maize fibre affects short-term calcium absorption in adolescent boys and girls: a randomised controlled trial using dual stable isotopic tracers

  • Corrie M. Whisner (a1), Berdine R. Martin (a1), Cindy H. Nakatsu (a2), George P. McCabe (a3), Linda D. McCabe (a3), Munro Peacock (a4) and Connie M. Weaver (a1)...

Abstract

Soluble maize fibre (SCF) has been found to significantly improve bone mineral density and strength in growing rats compared with several other novel prebiotic fibres. The objective of the present study was to investigate the effect of SCF on Ca absorption and retention in pubertal children by studying the potential absorption mechanisms of the intestinal microbiota. A total of twenty-four adolescent boys and girls (12–15 years) participated in two 3-week metabolic balance studies testing 0 g/d SCF (control (CON) treatment) and 12 g/d SCF (SCF treatment) in a random order by inclusion in a low-Ca diet (600 mg/d). Fractional Ca absorption was measured at the end of the two intervention periods using a dual-stable isotope method. Diet composites and faecal and urine samples were collected daily and analysed for Ca content. Ca retention was calculated as dietary Ca intake minus Ca excretion in faeces and urine over the last 2 weeks. Microbial community composition in the faecal samples collected at the beginning and end of each session was determined by 454 pyrosequencing of the PCR-amplified 16S ribosomal RNA gene. Fractional Ca absorption was 12 % higher (41 mg/d) after the SCF treatment compared with that after the CON treatment (0·664 (sd 0·129) and 0·595 (sd 0·142), respectively; P= 0·02), but Ca retention was unaffected. The average proportion of bacteria of the phylum Bacteroidetes was significantly greater in the participants after the SCF treatment than after the CON treatment. These results suggest that moderate daily intake of SCF, a well-tolerated prebiotic fibre, increases short-term Ca absorption in adolescents consuming less than the recommended amounts of Ca.

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Copyright

Corresponding author

* Corresponding author: C. M. Weaver, fax +1 765494 0674, email weavercm@purdue.edu

References

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1 Fulgoni, VL III, Keast, DR, Auestad, N, et al. (2011) Nutrients from dairy foods are difficult to replace in diets of Americans: food pattern modeling and an analyses of the National Health and Nutrition Examination Survey 2003–2006. Nutr Res 31, 759765.
2 Krebs-Smith, SM, Guenther, PM, Subar, AF, et al. (2010) Americans do not meet federal dietary recommendations. J Nutr 140, 18321838.
3 Abrams, SA, Griffin, IJ, Hawthorne, KM, et al. (2005) A combination of prebiotic short- and long-chain inulin-type fructans enhances calcium absorption and bone mineralization in young adolescents. Am J Clin Nutr 82, 471476.
4 Griffin, IJ, Davila, PM & Abrams, SA (2002) Non-digestible oligosaccharides and calcium absorption in girls with adequate calcium intakes. Br J Nutr 87, Suppl. 2, S187S191.
5 van den Heuvel, EG, Schoterman, MH & Muijs, T (2000) Transgalactooligosaccharides stimulate calcium absorption in postmenopausal women. J Nutr 130, 29382942.
6 Devareddy, L, Khalil, DA, Korlagunta, K, et al. (2006) The effects of fructo-oligosaccharides in combination with soy protein on bone in osteopenic ovariectomized rats. Menopause 13, 692699.
7 Weaver, CM, Martin, BR, Nakatsu, CH, et al. (2011) Galactooligosaccharides improve mineral absorption and bone properties in growing rats through gut fermentation. J Agric Food Chem 59, 65016510.
8 Zafar, TA, Weaver, CM, Zhao, Y, et al. (2004) Nondigestible oligosaccharides increase calcium absorption and suppress bone resorption in ovariectomized rats. J Nutr 134, 399402.
9 Scholz-Ahrens, KE, Acil, Y & Schrezenmeir, J (2002) Effect of oligofructose or dietary calcium on repeated calcium and phosphorus balances, bone mineralization and trabecular structure in ovariectomized rats. Br J Nutr 88, 365377.
10 Lobo, AR, Colli, C & Filisetti, TMCC (2006) Fructooligosaccharides improve bone mass and biomechanical properties in rats. Nutr Res 26, 413420.
11 Bongers, A & Van den Heuvel, EG (2003) Prebiotics and the bioavailability of minerals and trace elements. Food Rev Int 19, 397422.
12 Weaver, CM, Martin, BR, Story, JA, et al. (2010) Novel fibers increase bone calcium content and strength beyond efficiency of large intestine fermentation. J Agric Food Chem 58, 89528957.
13 Harris, J & Benedict, F (1919) A Biometric Study of Basal Metabolism in Man. Washington: Carnegie Institute of Washington.
14 Wu, L, Martin, BR, Braun, MM, et al. (2010) Calcium requirements and metabolism in Chinese-American boys and girls. J Bone Miner Res 25, 18421849.
15 Allen, LH, Raynolds, WL & Margen, S (1979) Polyethylene glycol as a quantitative fecal marker in human nutrition experiments. Am J Clin Nutr 32, 427440.
16 Whisner, CM, Martin, BR, Schoterman, MH, et al. (2013) Galacto-oligosaccharides increase calcium absorption and gut bifidobacteria in young girls: a double-blind cross-over trial. Br J Nutr 110, 12921303.
17 van den Heuvel, EG, Muys, T, van Dokkum, W, et al. (1999) Oligofructose stimulates calcium absorption in adolescents. Am J Clin Nutr 69, 544548.
18 Ariefdjohan, MW, Savaiano, DA & Nakatsu, CH (2010) Optimization of DNA extraction kits for PCR–DGGE analysis of human intestinal microbial communities from fecal specimens. Nutr J 9, 23.
19 Nossa, CW, Oberdorf, WE, Yang, L, et al. (2010) Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome. World J Gastroenterol 16, 41354144.
20 Caporaso, JG, Kuczynski, J, Stombaugh, J, et al. (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Meth 7, 335336.
21 Edgar, RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26, 24602461.
22 Caporaso, JG, Bittinger, K, Bushman, FD, et al. (2010) PyNAST: a flexible tool for aligning sequences to a template alignment. Bioinformatics 26, 266267.
23 Haas, BJ, Gevers, D, Earl, AM, et al. (2011) Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. Genome Res 21, 494504.
24 Griffin, IJ, Hicks, PDM, Heaney, RP, et al. (2003) Enriched chicory inulin increases Ca absorption mainly in girls with lower Ca absorption. Nutr Res 23, 901909.
25 Hamady, M, Lozupone, C & Knight, R (2010) Fast UniFrac: facilitating high-throughput phylogenetic analyses of microbial communities including analysis of pyrosequencing and PhyloChip data. ISME J 4, 1727.
26 Clavijo-Gutierrez, AP (2013) Response of human gut microbiota to diet supplementation with soy or soluble corn fiber. PhD Thesis, Purdue University.
27 Abrams, SA, Griffin, IJ & Hawthorne, KM (2007) Young adolescents who respond to an inulin-type fructan substantially increase total absorbed calcium and daily calcium accretion to the skeleton. J Nutr 137, 2524S2526S.
28 Raschka, L & Daniel, H (2005) Diet composition and age determine the effects of inulin-type fructans on intestinal calcium absorption in rat. Eur J Nutr 44, 360364.
29 Wastney, ME, Martin, BR, Peacock, M, et al. (2000) Changes in calcium kinetics in adolescent girls induced by high calcium intake. J Clin Endocrinol Metab 85, 44704475.
30 Maathuis, A, Hoffman, A, Evans, A, et al. (2009) The effect of the undigested fraction of maize products on the activity and composition of the microbiota determined in a dynamic in vitro model of the human proximal large intestine. J Am Coll Nutr 28, 657666.
31 Hooda, S, Boler, BM, Serao, MC, et al. (2012) 454 pyrosequencing reveals a shift in fecal microbiota of healthy adult men consuming polydextrose or soluble corn fiber. J Nutr 142, 12591265.
32 Davis, LM, Martinez, I, Walter, J, et al. (2010) A dose dependent impact of prebiotic galactooligosaccharides on the intestinal microbiota of healthy adults. Int J Food Microbiol 144, 285292.
33 Eeckhaut, V, Van Immerseel, F, Teirlynck, E, et al. (2008) Butyricicoccus pullicaecorum gen. nov., sp. nov., an anaerobic, butyrate-producing bacterium isolated from the caecal content of a broiler chicken. Int J Syst Evol Microbiol 58, 27992802.
34 Iino, T, Mori, K, Tanaka, K, et al. (2007) Oscillibacter valericigenes gen. nov., sp. nov., a valerate-producing anaerobic bacterium isolated from the alimentary canal of a Japanese corbicula clam. Int J Syst Evol Microbiol 57, 18401845.
35 Jumas-Bilak, E, Jean-Pierre, H, Carlier, JP, et al. (2005) Dialister micraerophilus sp. nov. and Dialister propionicifaciens sp. nov, isolated from human clinical samples. Int J Syst Evol Microbiol 55, 24712478.
36 Martinez, I, Kim, J, Duffy, PR, et al. (2010) Resistant starches types 2 and 4 have differential effects on the composition of the fecal microbiota in human subjects. PLoS ONE 5, e15046.
37 Walker, AW, Ince, J, Duncan, SH, et al. (2011) Dominant and diet-responsive groups of bacteria within the human colonic microbiota. ISME J 5, 220230.

Keywords

Soluble maize fibre affects short-term calcium absorption in adolescent boys and girls: a randomised controlled trial using dual stable isotopic tracers

  • Corrie M. Whisner (a1), Berdine R. Martin (a1), Cindy H. Nakatsu (a2), George P. McCabe (a3), Linda D. McCabe (a3), Munro Peacock (a4) and Connie M. Weaver (a1)...

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