Studies were continued to investigate the ability of secondary metabolites sequestered in Antarctic sponges to cause feeding deterrent responses (tube foot retractions) in a common predator of Aritarctic sponges, the sea star Perknaster fuscus. Lipophilic (dichloromethane/methanol) and hydrophilic (methanol/water) extracts of 15 new species of Antarctic marine sponges were tested for their ability to induce sustained tubefoot retraction. One additional species, Cinchyra antarctica, was re-tested from a previous study because we discovered a colour morph that was of comparative interest. Employing established protocols, sponge extracts were imbedded in silicone and presented to an extended tube-foot on the tip of a glass rod. Extracts of ten of the 15 new species of sponges (67%) caused significantly longer tube-foot retractions than controls. Among all sponges tested, significant tube-foot retraction activity was primarily associated with dichloromethane/methanol extracts (eleven sponge species or colour morphs), while significant tube-foot retraction activity was less common in response to methanol/water extracts (three sponges species or colour morphs). Both lipophilic and hydrophilic extracts from the sponges C. antarctica (yellow morph) and Scolymastia joubini elicited significant tube-foot retraction activity, suggesting that more than one compound from these speciesmight elicit tube-foot retractions in P. fuscus. Overall these findings lend considerable support to the hypothesis that there has been significant evolutionary selection for chemical defences among Antarctic marine sponges in McMurdo Sound, in contrast to earlier biogeographic selection models that predicted low levels of chemical defences in polar marine invertebrates.

In many Antarctic benthic communities, sponges can be considered as keystone species on both hard and soft bottoms, affecting community structure and sediment texture (Cattaneo-Vietti et al. 2000). Moreover, Antarctic sponges are known to be exploited by numerous organisms as atrophic source. The most important Antarctic sponge predators are asteroids and molluscs which move and digest slowly. The activity of invertebrate predators is not affected by the sponge nutritional composition: toxicity and relative abundance are considered to be the most important factors regulating predation on the Antarctic sponge fauna (McClintock 1987).

Land-fast ice in the vicinity of Adélie Land was sampled during spring 1995. The ice was annual, thin, with no consistent snow cover, and exposed to oceanic conditions. Temporal and spatial variations of the vertical pigment distribution were studied in relation to environmental factors, during the break up of the ice. Different levels were sampled in the congelation ice and the platelet ice-like layer (PLI). Under-ice water and open water masses were also sampled. The algal biomass was greater in the PLI (24 ±14 μg chl a l−1 offshore and up to 9 mg chl a l−1 near-shore), than in the under-ice water, and fell to 0.9 ± 0.64 μg chl al−1 in open water masses. Homogenous low pigment concentrations were detected in the upper levels of congelation ice. A gradient was identified along a 7 km seaward transect, sampled in November, with the lowest biomass offshore. The integrated pigment concentrations in fast ice reached very high levels 500 mg chl a m−2 near the coast and 0.8 mg m−2 offshore), with apparently no relationship with either the ice thickness or snow cover. In the congelation ice nutrient concentrations were low and their distribution homogenous, whereas in the PLI high concentrations of nitrate (up to 100–300 μM NO3) and silicic acid [30–100 μM Si(OH)4] were detected, often related to high pigment concentrations and proximity to islands. The sea ice algae communities were diverse, but mostly composed of chain-forming and tube-dwelling pennate diatoms (Amphiprora, Berkeleya, Nitzschia and Navicula). Cell densities in PLI reached up to 1010 cells l−1. At very low biomass and cell densities 2 104 cells l−1) the phytoplankton also had a low diversity; some species were similar to those of the PLI, such as Navicula glaciei, but other were typically planktonic (Chaetoceros). At sea ice break-up it is estimated that a significant proportion of particulate matter (up to 0.5 g chl a m−2 near-shore) was transferred to the underlying water masses (on an average 15 t POC km−1 shoreline).

Community analyses of the macrobenthos living on the Weddell Sea shelf revealed a distinct horizontal patchiness. Within some systematic groups a specific faunistic classification could clearly be defined, e.g. for asterozoans and holothurians. Whilst for fish, only a general zoogeographical pattern was discernible, there were some recognisable relationships to different microhabitats. The extreme differences in the distribution of sponges observed seems to reflect their highly variable biological characteristics. Studies using underwater imaging methods for benthic research have provided strong evidence for the ecological significance of two factors. The first, iceberg scouring, leads to a variety of simultaneous stages of recolonization, which result in an increase in beta-diversity. As a consequence, it is unlikely that regionally a stage approaching a theoretical climax will ever be attained. Secondly, the structural diversity of living substrata provides the basis for an additional variety of epibiotic species. Only weak or non-detectable correlations have been found between benthic assemblages and physical parameters, such as water depth, sediment type, bathymetric features and the abundance of deposited phytodetritus. This indicates a benthic system which is relatively uncoupled from processes in the water column. The combination of stable environmental conditions and disturbances taking place over long periods of time, which are partly a special feature of Antarctica's glacial history, shaped the diversity and faunal composition of the macrobenthos. Consequently, neither Houston's “intermediate-disturbance-hypothesis” nor Sander's “stability-time-hypothesis” can be rejected for this part of the Antarctic ecosystem.

The terrestrial vegetation in Antarctica is restricted to small, very isolated, ice-free areas on the continent and on the islands adjacent to the Peninsula region. Lichens, a symbiosis between a fungus and an alga or cyanobacterium, form the most prominent component in the vegetation. They have the greatest number of species compared with other cryptogamic and unicellular organisms. They are able to colonize all types of solid substrata and have a low mineral nutrient demand. Their physiological vigour is derived from high freezing tolerance and the ability to be photosynthetically activated by water vapour uptake from snow at temperatures as low as c. -20°C. Long-term monitoring in their natural habitats demonstrates that lichens are photosynthetically active at suboptimal temperatures. Locally, however, they can benefit from meltwater on insolated rocks, but only for short periods in early summer. Although at this time they may be exposed to substrate temperatures of > 20°C and strong light they do not suffer from photostress. If, in winter, lichens are covered and kept dark by snow with temperatures close to 0°C, lichens enter a negative carbon balance. This effect may be relevant to lichens under conditions of global or regional warming.

Specimens of 22 species of pycnogonids belonging to twelve genera and seven families were collected during a cruise near Livingston Island, South Shetland Islands. Twenty two of the 94 stations yielded pycnogonids: the new species Ammothea hesperidensis is described fully, illustrated, and compared with similar species. The family Nymphonidae provided both the greatest diversity of species (seven) and number of specimens (35). The most abundant species were Achelia hoekii and Nymphon australe.

In Argentina, the Nototheniidae is represented by about 20 species, which mostly live in coastal waters of the continental shelf and slope. A few species inhabit inshore waters along the coasts of Patagonia and the Fueguian and Falkland (Malvinas) archipelagos and even reach rocky intertidal zones (Norman 1937, Hart 1946). Patagonotothen sima (Richardson 1844) is distributed in the Atlantic from the San Matías Gulf (around 42°S) to the Beagle Channel (54°50'S), inhabiting shallow waters including the intertidal zones. During faunal surveys in the rocky intertidal zone in Puerto Deseado, Santa Cruz (by A. Gosztonyi), it was noted that males of P. sima, one of the most common fish species in that habitat, appeared to have a higher second dorsal fin than the females.

Two sediment cores taken from Jaw lake (Bunger Hills, East Antarctica) were analysed for diatom composition and abundance. A diatom-salinity transfer function developed for the nearby Vestfold Hills was used to determine palaeosalinity reconstructions from the assemblages preserved in each of the cores. There is a large step-wise decrease in salinity in the second core from at least c. 4000 to c. 2000 uncorrected 14C yr BP. The salinity record from the first core starts at c. 3000 uncorrected 14C yr BP and is comparable with the salinity of the second core between c. 3000 and c. 2000 uncorrected 14C yr BP. Sudden lake water dilution in both cores at c. 1900 uncorrected 14C yr BP is followed by brief increases in lake water salinity between c. 1900 and c. 500 uncorrected 14C yr BP, after which gradual dilution to present occurs. This analysis of the local environmental history of this saline lake reveals a mid–late Holocene evolution of the lake basin similar to that documented from earlier marine and freshwater lacustrine sediments in the Bunger Hills. The high coherence of the independent sediment records suggests a robust general palaeosalinity reconstruction of the lake is achieved in addition to providing evidence for the fidelity of single sediment cores as useful and adequate representation of the palaeolimnological histories of Antarctic lakes.

Quartz stone sublithic cyanobacterial communities are common throughout the Vestfold Hills, Eastern Antarctica (68°S 78°E) contributing biomass in areas otherwise devoid of any type of vegetation. In this study, the sublithic microbial community and underlying soil was investigated using a variety of traditional and molecular methods. Although direct epifluorescent counts of the sublithic growth (average 1.1 × 109 cells g−1 dry weight) and underlying soil (0.5 × 109 cells g−1 dry weight) were similar, sublith viable counts (2.1 × 107 cfu g−1 dry weight) were on average 3-orders of magnitude higher in the subliths. Enrichment and molecular analyses revealed the predominate cyanobacteria were non-halophilic, able to grow optimally at 15–20°C, and were related to the Phormidium subgroup with several distinct morphotypes and phylotypes present. Sublithic heterotrophic bacterial populations and those of underlying soils included mostly psychrotolerant taxa typical of Antarctic soil. However, psychrophilic and halophilic bacteria, mostly members of the alpha subdivision of the Proteobacteria and the order Cytophagales, were abundant in the sublithic growth film (20–40% of the viable count and about 50% of isolated individual taxa) but absent from underlying soils. It is suggested that quartz stone subliths might constitute a “refuge” for psychrophilic bacteria.

Molecular analysis of phytoplankton population structure has lagged behind other groups and has usually been inferred from physiological data determined from relatively few clones. Nearly every physiological measurement has shown that no single clone of any phytoplankton species can be considered truly representative of that species. One important reason why studies of phytoplankton population structure are perhaps 20 or more years behind those of other organisms is because of the necessity to establish clonal cultures prior to genetic analysis and the inability to perform fine-scale sampling under most conditions. Isozyme analysis, performed for a few species, has revealed heterozygosity between populations. In addition, fingerprinting analyses, such as Random Amplified Polymorphic DNAs (RAPDs) or multi-locus probes, have shown that phytoplankton blooms are not mono-clonal, are highly diverse and isolates are related by geographic origin. In the Southern Ocean, only two studies have been made of the population structure of phytoplankton. The first, based on quantitative genetic analysis of morphometric features, suggests that there is sufficient genetic variation in populations of Thalassiosira tumida to allow speciation in terms of major shifts in morphology under conditions of continued directional selection. The second, using sequence data from the noncoding regions of the internal transcribed spacer region (ITS) in the ribosomal cistron as a molecular marker, shows that populations of Phaeocystis antarctica within continental water masses are homogenous with little evidence of population structure. Populations found within the Antarctic Circumpolar Current are genetically distinct from others, suggesting the currents also play an important role in determining population structure in phytoplankton populations.

Pigment analysis (HPLC and fluorometry) and light microscopy observations of the gut content of Thelepus extensus (Terebellidae) and surrounding sediments were carried out at two hydrodynamically contrasting sites at subantarctic Îles Kerguelen; 1) a sheltered site (Port-Raymond) with a dense population of large-bodied animals, and 2) an exposed site (Port-aux-Français) with a scarce population of smaller individuals. Chlorophyll a derivatives (phaeophorbide a-like) were the most abundant pigments in sediments and polychaete digestive tracts. The second important group of pigments consisted of five unknown carotenoids of which two were present only in the polychaete digestive tract. Pigments in the muddy sediment at the sheltered site appeared to originate from the grazing activity of the polychaetes, as suggested by the high concentrations of degradation products and the same phaeophorbides observed both in the sediment and in the gut contents. Material originating from the dense Macrocystis pyrifera (Phaeophyceae) and Ulvae (Chlorophyceae) cover constituted a large part of the polychaetes' diet at this sheltered site, as indicated by macroalgal debris in the gut contents and the presence of violaxanthin in the sediment and lutein/zeaxanthin in both polychaetes and sediments. The polychaetes seem to adapt their grazing mode to the environmental conditions, preferring suspension feeding in the sheltered site and deposit feeding at the exposed site. The different morphologies, behaviours and life spans of the two conspecific populations may by linked to the contrasting hydrodynamics of the two sites and to their respective diets.

Sensitive High Resolution Ion MicroProbe (SHRIMP) U–Pb zircon dating of pegmatites from Mount Kirkby, northern Prince Charles Mountains, east Antarctica indicates felsic intrusive activity at 991 ± 22 Ma and 910 ± 18 Ma. Pegmatite emplacement occurred during prolonged high-grade early Neoproterozoic tectonism. These ages correlate well with previously published U–Pb zircon ages obtained from felsic intrusive bodies elsewhere within the northern Prince Charles Mountains. Early Palaeozoic activity at Mount Kirkby is restricted to the emplacement of minor planar pegmatites at 517 ± 12 Ma, which provide a maximum age for local development of discrete extensional mylonites. No conclusive evidence of tectonic or metamorphic events at c. 800 Ma and c. 500 Ma, which have been recently postulated for the region, can be identified from the presently available U–Pb zircon data.

Little information is available regarding predator-prey interactions in High-Antarctic coastal systems. In this study, the predation of Trematomus bernacchii (Pisces: Nototheniidae) on Adamussium colbecki (Mollusca: Pectinidae) is described and the related impact on the population structure of the mollusc is hypothesized. Fishes and scallops were collected during several expeditions between 1990/91 and 1997/98 summers, in nearshore waters at Terra Nova Bay (Antarctica). Adamussium colbecki was the main food item of T. bernacchii and an ontogenetic prey-size selection was observed. The predation was mainly on medium size classes of the scallop. These were lacking in the A. colbecki population sampled in the same period suggesting that the impact of fish-feeding on the size structure of the natural population of the mollusc may be substantial. Two size classes of the Adamussium population were not preyed on. Large adults avoid predation either because of the limits for mouth gape in the fish or by swimming avoidance capability, while smaller scallops may not be preyed upon because they are attached through byssus threads to very mobile large adults.

Fossil marine diatom assemblages from the Sørsdal Formation, a deposit of diatomaceous sand from Marine Plain in the Vestfold Hills, provide age and palaeoenvironmental information from a Pliocene coastal setting in East Antarctica. Benthic and meroplanktic diatom taxa suggest deposition in a shallow coastal embayment with water depths of 20–25 m. Diatom biostratigraphy indicates that the Sørsdal Formation belongs to the Fragilariopsis barronii Zone (4.5 to 4.1 Ma), through correlation to reference sections in the Ross Sea and Southern Ocean. Features of the other biota suggest higher sea-surface temperatures and reduced sea-ice cover, consistent with global records of higher sea level.

The majority of species of the Antarctic coastal fish fauna is represented today by the perciform suborder Notothenioidei. The separation of basal lineages of notothenioids has been estimated to have occurred between 23 and 22 million years ago (m.y.a.), while a major diversification probably occurred 16–10 m.y.a. Cytogenetic approaches aim to study the genomic change that, at the cell level, accompanied the radiation of this group. The information available for 66 of 120–130 species makes possible the description of the main patterns of chromosome diversification within the suborder. Within some families (Channichthyidae, Artedidraconidae) the range of the chromosomal variability seems to be minimal whilst the high karyotypic diversity of Nototheniidae and Bathydraconidae is consistent with morphological and molecular data suggesting the paraphyletic nature of these two taxa. Molecular cytogenetics allows detailed chromosome characterization, including mapping of ribosomal genes and of telomeric sequences, thus providing information on processes of karyotypic rearrangement and direction of chromosomal change. Active process of genomic restructuring leads to intraspecific variability in several species, at different levels of chromosomal organization. The growing amount of information make it possible to use notothenioids as a model for testing hypotheses of evolutionary change in marine organisms, including chromosomal diversification.

A macroflora from John Peaks, Powell Island, contains Sagenopteris nilssoniana, Cladophlebis oblonga, Brachyphyllum sp., Elatocladus confertus, and Sphenopteris sp. The macroflora is best correlated with the Botany Bay Group flora, suggesting an Early to Middle Jurassic age for the Powell Island Conglomerate. This age supports new interpretations for the geological evolution of the Antarctic Peninsula that suggest the initial phase of Gondwana break-up was manifested as small rift grabens with continental deposits.

Surface patterns of alternating snow and blue-ice bands are found in the Jutulgryta area of Dronning Maud Land, Antarctica. The snow-accumulation regions exist in the lee of blue-ice topographic ridges aligned perpendicular to winter winds. The snow bands are c. 500–2000 m wide and up to several kilometres long. In Jutulgryta, these features cover c. 5000 km2. These alternating snow and blue-ice bands are simulated using a snow transport and redistribution model, SnowTran-3D, that is driven with a winter cycle of observed daily screen-height air temperature, humidity, and wind speed and direction. The snow-transport model is coupled to a wind model that simulates wind flow over the relatively complex topography. Model results indicate that winter winds interact with the ice topographic features to produce alternating surface patterns of snow accumulation and erosion. In addition, model sensitivity simulations suggest that subtle topographic variations, on the order of 5m elevation change over a horizontal distance of 1 to 1.5 km, can lead to snow-accumulation variations that differ by a factor of six. This result is expected to have important consequences regarding the choice of sites for ice-coring efforts in Antarctica and elsewhere.

Local earthquakes recorded at the King Sejong station (62° 13′31″S, 58° 47′07″W) from 1995–96 have been analysed to study the seismicity and tectonics around the northern Antarctic Peninsula. The nature of shallow-focused normal fault earthquakes along the South Shetland Platform is still unclear. Dominant normal fault earthquakes and minor strike-slip earthquakes in the Eastern Bransfield Basin suggest 1) ongoing extension, and 2) transtensional stress transmitted from the Antarctic–Scotia transform boundaries, the South Scotia Ridge and the Shackleton Fracture Zone. A lack of seismicity in the Central Bransfield Basin supports that active seismicity in the Eastern Bransfield Basin is not a result of subduction along the South Shetland Trench. Shallow focused earthquakes have been observed along the NW–SE trending gravity low line between the Central and the Eastern Bransfield Basins that approximately coincides with the landward projection of a fracture zone in the former Phoenix Plate.

A census of pelagic copepods identifies 346 species from 38 families (i.e. 16.7% of all copepods). Among them, 289 species are considered “cosmopolitan” and 57 “endemic”. Most of the species were described in the late 19th or early 20th century. However, due to a resurgence of interest in biological diversity, a large number of exhaustive revisions at various taxonomic levels have recently been initiated. One hundred and thirteen documents covering the census and distribution of copepod species are analysed. The distribution of species in the South Seas is given for 24 sectors chosen in relation to the geographical coordinates of the South Atlantic, South Pacific and Southern Indian oceans and their location with respect to the Antarctic Convergence or the continental shelf. The respective roles of Lagrangian displacement, of the hydrological features characterising the Southern Ocean and of the general ocean currents are emphasised to explain the scattered distributions of the species. Twenty-one species are catalogued as bipolar, 46 species as specifically sub-Antarctic and 222 cosmopolitan ones are probably from sub-tropical/temperate regions.

A light, mining drill rig deployed from the stern of a research vessel has been used to carry out shallow drilling in 212 m water depth on the continental shelf in the eastern Weddell Sea. Penetration was 15 m below the seabed with 18% recovery in the 31 hours available for the experiment. The recovered glacigenic sediments are predominantly volcanic material of basaltic and andesitic composition with petrological characteristics and age similar to the continental flood basalts exposed in Vestfjella, about 130 km upstream from the drill site. The sediments include a reworked marine Miocene diatom flora. The material documents oscillations of the East Antarctic Ice Sheet over the past 30 ka. The lowermost diamicton probably represents a deformation till, and the grounding line retreated past the drill site 30 km from the shelf edge about 30 kyr BP. A readvance occurred during the Late Wisconsin Glacial Maximum. Assuming a reservoir correction of 1300 yr, marine conditions existed at the site between 10.1-7 kyr BP, and later at least between 2.8 and 2.5 kyr BP. The stratigraphy at the site has been disturbed by iceberg ploughing and/or contact between the ice shelf and the sea floor during local advances after 2.5 kyr BP.