The advent of the open access (OA) movement in publishing has been instrumental in causing a shift in the accessibility of research findings published in academic journals. The adoption of OA and other online publication models means that the results of scientific research published in journals using a free access (FA) framework are now available, free of charge, to anyone with access to the Internet. FA journals typically require a payment from the authors of a manuscript, which has raised concerns about the quality of work published in them; accepting payment from an author may compromise a journal’s acceptance criteria. This study addresses whether journal policy on the treatment of animals is influenced by whether a journal follows a FA publishing model, and whether a requirement to pay for publication has an influence. A random sample of 332 biomedical journals listed in the ISI Web of Knowledge and Directory of Open Access Journals databases were assessed for whether they had an ethical policy on publishing animal studies, and what form of publication framework they used (103 of the journals followed a FA framework; 101 charged in some way for publication). Only 135 (40.7%) of the journals surveyed demanded that submissions comply with a pre-defined ethical stance. FA journals are just as likely to have an ethical policy on the treatment and presentation of animal studies as ‘traditional’, non-FA journals (significance of there being a difference: P = 0.98), and there is no relationship between policy and whether an author is required to pay for publication (significance of there being a difference: P = 0.57). Older journals are more likely to have an ethical policy (P = 0.03). There is, therefore, no obvious compromise shown by FA journals in the explicit policies on reporting studies involving animals. However, since anyone can read published FA studies online, FA journals that do not have an explicit policy about publishing animal research are urged to consider adopting one.

In this study, we investigate the extent to which the incidence of Escherichia coli O157:H7 can be predicted in human faeces, from human intake and infection via water contaminated by livestock and carrying this zoonotic pathogen in North-East (NE) and South-West (SW) regions of Scotland. In SW Scotland, there is a risk of coastal recreational waters failing EU standards for faecal indicator organisms, and this is assumed to be the main potential waterborne route of infection. In NE Scotland, the main waterborne route is assumed to be the many private drinking water supplies; these are mainly derived from shallow groundwater and surveys show that there is potential for significant levels of microbial contamination from livestock. The risk to human health from these sources has been assessed using a combination of process models, epidemiological risk-assessment methods and survey data. A key assumption in the calculations is the amount of mixing of pathogenic and non-pathogenic E. coli between animal faecal sources and contaminated water intake by humans. Using the probability distributions of the E. coli O157 content of individual faecal pat material (which would imply no mixing between source and human intake), based on three recent surveys of animal faeces in Scotland, led to predicted annual risks of infection slightly higher than observed human infection incidence. Using the geometric mean to represent partial mixing (which theoretically may over- or underestimate incidence with a concave dose–response curve) gave infection rates similar to those observed for two of the three faecal surveys. Using the arithmetic mean led to over-prediction of risk. This is to be expected if the true dose–response curve is (such as the Beta-Poisson curve used here) concave. Other factors that may lead to over-prediction of incidence are discussed, including under-reporting, loss of infectivity as a result of environmental exposure, immunity and the appropriateness of the Beta-Poisson curve. It is concluded that better epidemiological data for calibration of the dose–response curve, better knowledge of the degree of mixing and understanding of immunity are key requirements for progress in process model-based predictions of infection rate. The paper also explores the potential of improved farm and catchment scale management to deliver cost-effective mitigation of pollution of bathing and drinking water by livestock zoonoses.

The time constraints of the classic twice-daily milking routine are less easily endured by individual dairy farmers, because of their impact on quality of life. Our aim was to evaluate milk production responses by dairy cows milked twice daily at contrasting intervals. In experiments 1 (20 cows) and 2 (28 cows), four milking regimes were compared during a 3-week period beginning after the peak of lactation. Three groups of five cows were milked twice daily (TDM) with milking intervals of 11 : 13, 7 : 17 and 3 : 21 h in experiment 1, and three groups of seven cows at 11 : 13, 5 : 19 and 2.5 : 21.5 h in experiment 2. One group (five and seven cows respectively) was milked once daily (ODM) in each experiment. In experiment 3 (three groups, 12 cows per group), one group was milked at 10 : 14 h and one at 5 : 19 h, and the third group once daily. Milking treatments began during the second week of lactation and continued for an average of 23 weeks. In experiments 1 and 2, daily milk yields were reduced by 4.1%, 11.5% and 28%, for the 5 : 19, 3 : 21 and ODM milking treatments compared with the 11 : 13 h interval. In experiment 3, the decrease in daily milk yields for 5 : 19 h and ODM was 10% and 40% compared with the 10 : 14 h time interval. In the average daily milk, fat and protein contents and somatic cell counts were not different between the TDM groups, and the ODM group had (or tended to have) a higher fat and protein content. For a given milking, milk fat content decreased from about 60 to 32 g/kg as the preceding milking interval increased from 2.5 to 3 h up to 12 h. It then levelled out and even increased, mainly after 18 to 20 h. Somatic cell count showed a similar trend, and protein content did not change steadily. Dry matter intake, body weight and body condition score were not affected by contrasting milking intervals. After resumption of TDM with conventional intervals, productions of milk, fat and protein no longer differed between the TDM groups. Milk yield of previously ODM cows remained lower by 2 kg/day (P = 0.15) in experiments 1 and 2, and by 7 kg/day (P < 0.05) in experiment 3. These results suggest that TDM at contrasting intervals up to 5 : 19 h is feasible as it decreases milk yield only moderately, especially if implemented from peak of lactation.

The last decade has seen important developments in the use of carotenoid pigments to authenticate pasture-feeding in ruminants. However, dehydrated alfalfa is sometimes incorporated in grain-based concentrates fed to stall-raised lambs, which may affect the reliability of the pasture-feeding authentication methods based on carotenoids in plasma and fat, due to significant residual carotenoid levels post-dehydration. The aim of this study was to examine whether other compounds can give additional information to authenticate diet and discriminate pasture-fed lambs from lambs fed high levels of alfalfa indoors. Two feeding treatments were compared: pasture-feeding (P) v. stall-feeding with dehydrated alfalfa (A). Each treatment group consisted of seven male Romanov × Berrichon lambs. Pasture-fed (P) lambs grazed a permanent graminaceae-rich pasture maintained at a leafy, green stage, offered ad libitum; they received no supplementation at pasture. A-group lambs were individually penned and fed dehydrated alfalfa and straw; their feed level was adjusted to achieve a similar growth pattern as for P-group lambs. Plasma carotenoid concentration was measured at slaughter by spectrophotometry. The reflectance spectrum of perirenal and subcutaneous caudal fat was measured at 24-h post mortem and used to calculate an index (absolute value of the mean integral (AVMI)) quantifying light absorption by carotenoid pigments present in the fat. The nitrogen (N) stable isotopes ratio (δ15N) in both feed and longissimus dorsi muscle was measured by isotopes ratio mass spectrometry (IRMS). Volatile compounds were analyzed in perirenal fat for five randomly chosen lambs per treatment, using dynamic headspace–gas chromatography–mass spectrometry. Plasma carotenoid concentration and AVMI of the fat did not differ significantly between P- and A-group lambs, but there were significant between-treatment differences in meat δ15N values and in the terpene profiles of perirenal fat. A discriminant analysis performed using three compounds in different animal tissues (δ-cadinene in perirenal fat, δ15N value of the meat and plasma carotenoid concentration) clearly separated pasture-fed lambs from lambs fed high levels of alfalfa indoors.

The study involved 120 crossbred ewes (sixty 1.5 years old animals and sixty 2.5 years old animals; initial liveweight 67.6 kg, condition score 3.7), that were mated in October. They were assigned to six treatments (two shearing treatments (shorn and unshorn) × two silage feed values (low and medium) and two extended grazed herbage allowances (1.0 and 1.8 kg dry matter (DM)/day)) designed to evaluate the effects of shearing at housing, grass silage feed value and extended-grazed herbage allowance on their performance and the performance of their progeny. Swards, which had silage harvested on 6 September, received fertiliser N (34 kg/ha) for extended (deferred) grazing between 19 December and lambing in mid-March. The herbage was allocated at DM allowances of 1.0 or 1.8 kg/ewe daily until 1 February. For the final 6 weeks of pregnancy, daily herbage DM allowances were 1.5, 1.6, 2.0, 2.0 and 2.0 kg for weeks 6, 5, 4, 3 and 2 to parturition, respectively. Two grass silages (low and medium feed value) were offered from housing on 19 December to lambing in mid-March. At housing, half the ewes were shorn whilst the remainder remained unshorn. Each ewe received 23.4 kg concentrate prior to lambing. For the extended-grazed herbage and the low and medium feed-value silages, DM concentrations were 132, 225 and 265 g/kg, and metabolisable energy (ME) concentrations were 10.0, 10.0 and 10.7 MJ/kg DM, respectively. Treatment did not alter (P > 0.05) litter size or number reared. Grass silage feed value did not significantly alter silage DM intake, or ewe and subsequent lamb performance. Increasing herbage allowance in mid-pregnancy decreased herbage utilisation (P < 0.05) and increased herbage intake (P < 0.05). Shearing increased silage intake (P < 0.05), lamb birth weight (P < 0.01) and tended to increase lamb weaning weight (P = 0.07). Relative to the housed shorn ewes, extended grazing did not alter (P > 0.05) ewe or subsequent lamb performance. It is concluded that shearing ewes at housing increased lamb birth weight due to increased silage intake probably associated with cold stress immediately post shearing and reduced heat stress in late pregnancy. Based on differences in lamb weight at weaning 0.8 kg of grass silage DM intake had the same feed value as a daily extended herbage DM allowance of 1.8 kg per ewe throughout the study. Neither silage feed value nor herbage allowance in mid-pregnancy affected lamb birth weight or subsequent growth rate.

The objective of this study was to evaluate the possibility of prediction of intramuscular fat (IMF) in live pigs using ultrasound method. Moreover, the accuracy of prediction at five different ultrasound intensity levels was investigated. Cross-sectional images of longissimus dorsi muscle (LD) at right last rib area, from hybrid pigs, were taken. Each pig was scanned at the same frequency (3.5 MHz) and at the five different ultrasound intensity levels 70%, 75%, 80%, 85% and 90% of total amplifying of sonograph, using the device ALOKA SSD-500. The video image analysis was used to predict IMF content (ultrasound intramuscular fat (UIMF) 70 to UIMF90). The second day after slaughter, the dissection of right half carcass was done. A sample of LD at the last rib was taken for laboratory analysis of IMF content (LAIMF). Scatter plots with UIMF on the x-axis and LAIMF on the y-axis were constructed to account for individual variability within and between intensity levels. Correlations between LAIMF and UIMF were significantly different from zero (r = 0.40–0.52), except for correlation between LAIMF and UIMF90 (r = 0.14). Statistical model with LAIMF (the dependent variable), UIMF (the same model for each intensity level), live weight (the covariates) and sex (the fixed effect) was developed. Coefficients of determination (R2) were 0.33, 0.38, 0.34, 0.25 and 0.17 with UIMF at the intensity level 70%, 75%, 80%, 85% and 90%. Root mean square errors ranged from 0.516% to 0.639%. Standard errors of individual prediction ranged from 0.523% to 0.649%. Goodness-of-fit of the model was also justified by testing the residuals for normality. Although the results are not quite unequivocal in favour of the one intensity level, it seems that intensity levels 75% and 80% are the most suitable to predict IMF in live pigs. Further research is needed, mainly to increase accuracy of collecting, processing and evaluating the sonograms using video image analysis.

A trial was conducted to study the effect of sire line (Duroc (DU) and Pietrain (PI)) on carcass, meat and fat quality of pigs reared outdoor and destined to dry-cured meat production. No differences between sire genotypes were detected in carcass fat thickness (P > 0.10) but carcasses from DU-sired pigs were longer (P < 0.05) and tended to have a higher yield of trimmed shoulders (P = 0.07) and hams (P = 0.06) than carcasses from PI-sired pigs. Loins from DU-sired genotype showed higher (P < 0.05) L* value and lower (P < 0.01) a* value than loins from PI-sired genotype. Pork from DU-sired offspring tended to have higher (P = 0.09) intramuscular fat (IMF) percentage and lower (P < 0.05) moisture proportion than meat from PI-sired offspring. Also, loins from DU-sired pigs had lower (P < 0.001) thawing losses than loins from PI-sired pigs. The subcutaneous fat from the DU-sired line tended to show lower (P = 0.08) percentage of total polyunsaturated fatty acids (PUFAs) than that from the PI-sire line, mostly due to the higher proportion of C18:2 (P = 0.09) and C20:3 (P < 0.01). However, no effect of crossbreed was detected on the total proportion of saturated, monounsaturated fatty acid (MUFAs) or PUFAs of IMF (P > 0.10). We conclude that both sire lines can be used successfully under outdoor conditions but DU boars are more adequate than PI boars for the production of heavy pigs intended for the dry-cured meat industry.

This study investigated the effects of pelvic suspension on the meat quality of M. longissimus dorsi, M. semimembranosus and M. adductor from 35 heifers with at least 75% Charolais breeding. Two-thirds of the heifers were slaughtered directly from pasture at 18 months of age and one-third was finished indoors and slaughtered at 22 months. After slaughter and electrical stimulation one side of each carcass was re-suspended by either the achilles tendon or the pelvic bone. Longissimus muscles were aged 7 or 14 days and were then evaluated for drip loss, colour, shear force and sensory analysis. As compared to 18-month-old heifers, 22-month-old heifers were heavier, more muscular and fatter (P < 0.05). Intramuscular fat content increased with slaughter age (P < 0.003). Pelvic suspension reduced longissimus peak force values, total energy, pH and thawing losses (P < 0.05) in heifers slaughtered at 18 months. Semimembranosus showed the largest response to pelvic suspension with significantly lower peak force and total energy values. Finishing for 4 months did not affect longissimus shear forces. Achilles-suspended samples had lower shear force values after 14 v. 7 days of ageing. Pelvic-suspended samples aged 7 days were, however, just as tender as those aged 14 days. Sensory analysis of longissimus samples aged 14 days showed that samples from pelvic-suspended sides had higher tenderness, lower bite resistance, more meaty taste and less visible marbling compared with samples from achilles-suspended carcasses.

The main aim of the present study was to examine the economic consequences of a reduction in the incidence of clinical mastitis (CM) at herd level under current Swedish farming conditions. A second objective was to ask whether the estimated cost of CM alters depending upon whether the model reflects the fact that in different stages of lactation, CM gives rise to different yield-loss patterns or postulates just one type of yield-loss pattern irrespective of when, during lactation, CM occurs. A dynamic and stochastic simulation model, SimHerd, was used to study the effects of CM in a herd with 150 cows (9000 kg of energy-corrected milk per cow-year). Four herd types, defined by production level and reproductive performance, were modelled to investigate possible interactions between herd type and response to a reduction in the risk of CM. Technical and economic results, given the initial incidence of CM (25.6 per 100 cow-years), were studied together with the consequences of reducing the initial risk of CM by 50% and 90% throughout lactation and the consequences of reducing the initial risk by 50% and 90% before peak yield. A conventional way of modelling yield losses – i.e. one employing a single yield-loss pattern irrespective of when, during the lactation period, the cow develops CM – was compared with a new modelling strategy in which CM was assumed to affect production differently depending on its lactational timing. The effect of the choice of reference level when estimating yield losses was investigated by comparing the results obtained using the potential yield of mastitic cows, had they not developed CM, with those obtained using the yield of non-mastitic cows. The yearly maximum avoidable cost of CM at herd level was estimated at €14 504, corresponding to 6.9% of the net return given the initial incidence of CM. Expressed per cow-year, the maximum avoidable cost was €97. The cost per case of CM was estimated at €428. Herd types all responded in a similar manner to the reduced relative risk of CM. There were no major differences in the results obtained using the new and the conventional modelling strategy, with the exception of the cost per case of CM. Similarities between the results obtained using the two methods were particularly evident when the mastitic cows’ own yield level, had they not developed CM, was used as the reference for production in healthy cows when yield losses were estimated. It was concluded that the conventional way of modelling yield losses is adequate and should, for the foreseeable future, be used in decision support systems.

A total of 200 crossbred pigs (castrated males and females) were used in five replicates to evaluate the influence of rearing conditions for fattening pigs on growth performance, manure production and gaseous emissions. Approximately at 36 kg body weight (BW), littermates were allocated to either a conventional (fully slatted floor, 0.65 m2/pig, considered as control, CON) or an alternative (sawdust bedding, 1.3 m2/pig, with free access to an outdoor area 1.1 m2/pig, OUT) system, until slaughter at approximately 115 kg BW. Pigs had free access to standard growing and finishing diets. Manure was stored as slurry below the slatted floor in the CON system and as litter, for the inside area, or slurry and liquid, for the outside area, in the OUT system. The amount and composition of manure were determined at the end of each replicate. Ammonia emission from the rooms was measured continuously. Dust and odour concentrations were measured in replicates 1 and 2, and CH4, N2O and CO2 emissions were measured in replicate 3. Compared with the CON, the OUT pigs exhibited a faster growth rate (+8%, P < 0.001) due to their greater feed intake (+0.21 kg/day, P < 0.01), resulting in a heavier BW (+7.3 kg, P < 0.001) and a lower lean meat content (−1.6% points, P < 0.001) at slaughter. The total amount of manure produced per pig was similar in both systems (380 kg/pig), but because of the contribution of sawdust, dry matter (DM) content was higher (P < 0.001) and concentrations in N, P, K, Cu and Zn in DM were lower (P < 0.001) in manure from the OUT than from the CON system. In the OUT system, most of the manure DM (70%) was collected indoor, corresponding mostly to the contribution of the sawdust, and most of the manure water (70%) was collected outdoor. Pigs excreted indoor about 60% and 40% of urine and faeces, respectively. Ammonia emission from the room was lower for the OUT system, whereas total NH3 emissions, including the outdoor area, tended to be higher (12.0 and 14.1 g/day N-NH3 per pig for CON and OUT, respectively). Nitrous oxide emission was higher (1.6 and 4.6 g/day N-N2O per pig for CON and OUT, respectively) and methane emission was lower (12.1 and 5.9 g/day per pig for CON and OUT, respectively), for the OUT compared with the CON system.

A 4-year interdisciplinary study was performed to compare two organically managed sheep production systems, using 118 ewes and 24 ha each. The systems differed in the ewes’ reproduction rhythm: one lambing per ewe per year (1L/1Y), with the aim of balancing feed self-sufficiency and lamb marketing periods v. three lambings over 2 years (3L/2Y), with the aim of maximising ewes’ productivity. The sustainability was evaluated through ewes’ reproductive performance, lamb growth rate, carcass characteristics and quality, animal health, forage and feed self-sufficiency, soil mineral balance and gross margin. General animal health was assessed by recording ewe and lamb mortality and putative cause of death. Nematode digestive-tract strongyles parasitism was studied using faecal egg counts and necropsies. Carcass quality was assessed by recording carcass weight, conformation and fatness, and colour and firmness of subcutaneous fat. Thirty-three percent of 3L/2Y ewes lambed twice a year against 4% of 1L/1Y. Mean ewe productivity was 161.3% and 151.0% in 3L/2Y and 1L/1Y, respectively, and it was more variable between years in 3L/2Y. Average concentrate feed consumption and the corresponding cost per ewe were higher in 3L/2Y than in 1L/1Y (156 v. 121 kg, and €49.5 v. €39.3, respectively). Finally, average gross margin was lower in 3L/2Y than in 1L/1Y (€59 v. €65 per ewe, respectively). Even in year 2002, when ewe productivity was highest in 3L/2Y (193%), gross margin was not different between systems (€90 v. €86 per ewe in 3L/2Y and 1L/1Y, respectively), because of higher concentrate costs in 3L/2Y. The 3L/2Y animals presented a lower health status, with a higher lamb mortality (P < 0.05) and a higher digestive-tract strongyles and coccidia parasitism level (P < 0.05). Lamb carcass conformation, fatness and fat colour were not different between systems, but carcass weight and subcutaneous dorsal fat firmness were lower in 3L/2Y lambs than in 1L/1Y lambs (P < 0.05 and < 0.001, respectively). Intensification in an organically managed sheep system, through an increased reproduction rhythm, thus did not lead to better economic results and proved riskier, more variable and more difficult to manage, and so less sustainable. The less intensive system (1L/1Y) was both highly efficient from the animal standpoint and highly feed self-sufficient. The technical and economic results of this system were better than those of organic private farms in the same area and matched those of non-organic farms.

The first part of this study was a cross-sectional analysis of the impact of 29 management factors on udder health in organic dairy farms in Switzerland. All 77 farms joined the extension program ‘pro-Q’. As a measure of udder health the theoretical bulk milk somatic cell count (TBMSCC) calculated by the monthly cow composite somatic cell count over a time period of 1 year was chosen. The basic udder health of the farms was determined by TBMSCC during the year prior to the start of the project (mean for all farms = 176 460 cells/ml). In the multivariable analysis, the five factors ‘swiss brown breed’, ‘alpine summer pasturing’, ‘calf feeding with milk from mastitis diseased cows’, ‘hard bedding’ and ‘no post-milking’ remained as significant risk factors on udder health. In the second part of the study, the development of management factors and the udder health situation affected by an extension program after 1 year was investigated. A partial improvement of the management factors on the farms but no overall improvement on udder health and no association between management changes and udder health changes were found. Improvement of udder health was more likely in farms with higher basic TBMSCC than in those farms with less udder health problems at the beginning of the project.

The present study was aimed to compare the developmental changes of carcass composition, meat quality characteristics and organ weight in pigs of different breeds. Six pigs (sex balance) of each breed were slaughtered at 35, 80 and 125 days of age, respectively. The carcass was chilled and the left carcass side was dissected into bone, lean meat, fat and skin; additionally, organ weight and meat quality parameters were observed. Carcasses of the Jinhua pig were lighter (P < 0.001), contained less lean meat percentage (P < 0.01) and more carcass fat percentage (P < 0.05) than did carcasses of the Landrace. L*-values were lower in Jinhua pigs than in Landrace at 125 days of age (P < 0.05), but the Jinhua pig had higher a*-values compared with Landrace at the age of 80 days (P < 0.01) and 125 days (P < 0.01), respectively. In addition, Jinhua pigs showed lower colour scores (P < 0.05), higher intramuscular fat (IMF) percentage (P < 0.05), less marbling scores (P < 0.05) and lower drip loss (P < 0.05) than Landrace. For organ weight, Jinhua pigs had higher relative heart weight at the age of 80 days (P < 0.05) and 125 days (P < 0.001), and higher relative liver weight at 125 days of age (P < 0.01) than that of Landrace. In addition, the relative kidney weight was heavier (P < 0.001) in the Jinhua pig than in the Landrace during the whole experiment. These results indicated that developmental changes of carcass composition, meat quality parameters and organ weight displayed breed differences. Jinhua pigs were fatter than Landrace but the former had better quality characteristics in the meat.