Hostname: page-component-76fb5796d-25wd4 Total loading time: 0 Render date: 2024-04-26T19:46:03.964Z Has data issue: false hasContentIssue false
  • English
  • Français

Microarray analysis of genes differentially expressed in the liver of lean and fat chickens

Published online by Cambridge University Press:  19 November 2009

H. B. Wang ,
Q. G. Wang ,
X. Y. Zhang ,
X. F. Gu ,
N. Wang ,
S. B. Wu  and
H. Li
H. B. Wang*
Affiliation:
College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China
Q. G. Wang
Affiliation:
College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China
X. Y. Zhang
Affiliation:
Ministry of Education Key Laboratory of Bioinformatics, School of Biomedicine, Tsinghua University, Beijing, 100084, P.R. China
X. F. Gu
Affiliation:
Harbin Gene-Tech Biochip Development Inc., LTD, Harbin, Heilongjiang 150090, China
N. Wang
Affiliation:
College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China
S. B. Wu
Affiliation:
School of Environmental and Rural Science and The Institute of Genomics and Bioinformatics, The University of New England, Armidale, Australia
H. Li*
Affiliation:
College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China
*
†E-mail: lihui@neau.edu.cn or lihui645@hotmail.com
Get access

Abstract

Excessive accumulation of lipids in the adipose tissue is one of the main problems faced by the broiler industry nowadays. In chicken, lipogenesis occurs essentially in the liver, in which much of the triglycerides that accumulate in avian adipose tissue are synthesized. In order to better understand the gene expression and its regulation in chicken liver, the gene expression profiles of liver at developmental stages of chicken (1 week, 4 weeks and 7 weeks of age) were investigated and differentially expressed genes between lean and fat chicken lines divergently selected for abdominal fat content for eight generations were screened. Our data indicated that 4 weeks of age was a very important stage on chicken liver lipogenesis compared to 1 week and 7 weeks of age, and the glycometabolism in chicken liver could be related to lipid metabolism and the difference of glycometabolism could be another potential reason for the fat and lean phenotype occurrence besides the difference of lipogenesis in chicken liver. Our result have established groundwork for further study of the basic genetic control of chicken obesity and will benefit chicken research communities as well as researches that use chicken as a model organism for developmental biology and human therapeutics.

Keywords

chickenlivergene expression profilelipid metabolism
Type
Full Paper
Information
animal , Volume 4 , Issue 4 , April 2010 , pp. 513 - 522
Copyright
Copyright © The Animal Consortium 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

These authors contributed equally to this paper.

References

Bhattacharyya, N, Chattapadhyay, R, Oddoux, C, Banerjee, D 1993. Characterization of the chicken apolipoprotein A-I gene 5′-flanking region. DNA and Cell Biology 12, 597–604.CrossRefGoogle ScholarPubMed
Bourneuf, E, Hérault, F, Chicault, C, Carré, W, Assaf, S, Monnier, A, Mottier, S, Lagarrigue, S, Douaire, M, Mosser, J, Diot, C 2006. Microarray analysis of differential gene expression in the liver of lean and fat chickens. Gene 372, 162–170.CrossRefGoogle ScholarPubMed
Burt, DW 2002. Applications of biotechnology in the poultry industry. World’s Poultry Science Journal 58, 5–13.CrossRefGoogle Scholar
Carre, W, Bourneuf, E, Douaire, M, Diot, C 2002. Differential expression and genetic variation of hepatic messenger RNAs from genetically lean and fat chickens. Gene 299, 235–243.CrossRefGoogle ScholarPubMed
Chambers, JR 1990. Genetics of growth and meat production in chickens. In Poultry Breeding and Genetics (ed. RD Crawford), no. 22, pp. 599–643. Elsevier, Amsterdam Press, The Netherlands.Google Scholar
Cogburn, LA, Wang, X, Carre, W, Rejto, L, Aggrey, SE, Duclos, MJ, Simon, J, Porter, TE 2004. Functional genomics in chickens: development of integrated-systems microarrays for transcriptional profiling and discovery of regulatory pathways. Comparative and Functional Genomics 5, 253–261.CrossRefGoogle ScholarPubMed
Daval, S, Lagarrigue, S, Douaire, M 2000. Messenger RNA levels and transcription rates of hepatic lipogenesis genes in genetically lean and fat chickens. Genetics Selection Evolution 32, 521–531.CrossRefGoogle ScholarPubMed
Gavrilova, O, Haluzik, M, Matsusue, K, Cutson, JJ, Johnson, L, Dietz, KR, Nicol, CJ, Vinson, C, Gonzalez, FJ, Reitman, ML 2003. Liver peroxisome proliferator-activated receptor gamma contributes to hepatic steatosis, triglyceride clearance, and regulation of body fat mass. Journal of Biological Chemistry 278, 34268–34276.CrossRefGoogle ScholarPubMed
Griffin, HD, Guo, K, Windsor, D, Butterwith, SC 1992. Adipose tissue lipogenesis and fat deposition in leaner broiler chickens. Journal of Nutrition 122, 363–368.CrossRefGoogle ScholarPubMed
Grisoni, ML, Uzu, G, Larbier, M, Geraert, PA 1991. Effect of dietary lysine level on lipogenesis in broilers. Reproduction, Nutrition, Development 31, 683–690.CrossRefGoogle ScholarPubMed
Guan, Y, Zhang, Y, Breyer, MD 2002. The role of PPARs in the transcriptional control of cellular processes. Drug News and Perspectives 15, 147–154.CrossRefGoogle ScholarPubMed
Kessler, AM, Snizek, PN Jr, Brugalli, I 2000. Manipulação da quantidade de gordura na carcaça de frangos. In Anais da Conferência APINCO de Ciência e Tecnologia Avícolas. Brazil Press, APINCO, Campinas, SP, Brazil, pp. 107–133.Google Scholar
Kotani, K, Peroni, OD, Minokoshi, Y, Boss, O, Kahn, BB 2004. GLUT4 glucose transporter deficiency increases hepatic lipid production and peripheral lipid utilization. The Journal of Clinical Investigation 114, 1666–1675.CrossRefGoogle ScholarPubMed
Majerus, PW, Kilburn, E 1969. Acetyl coenzyme A carboxylase. The roles of synthesis and degradation in regulation of enzyme levels in rat liver. Journal of Biological Chemistry 244, 6254–6262.CrossRefGoogle ScholarPubMed
Matsusue, K, Haluzik, M, Lambert, G, Yim, SH, Gavrilova, O, Ward, JM, Brewer, B Jr, Reitman, ML, Gonzalez, FJ 2003. Liver-specific disruption of PPAR gamma in leptin-deficient mice improves fatty liver but aggravates diabetic phenotypes. The Journal of Clinical Investigation 111, 737–747.CrossRefGoogle Scholar
Noy, Y, Sklan, D 2001. Yolk and exogenous feed utilization in the posthatch chick. Poultry Science 80, 1490–1495.CrossRefGoogle ScholarPubMed
O’Hea, EK, Leveille, GA 1968. Lipogenesis in isolated adipose tissue of the domestic chick (Gallus domesticus). Comparative Biochemistry and Physiology 26, 111–120.CrossRefGoogle ScholarPubMed
Peters, JM, Rusyn, I, Rose, ML, Gonzalez, FJ, Thurman, RG 2000. Peroxisome proliferator-activated receptor alpha is restricted to hepatic parenchymal cells, not Kupffer cells: implications for the mechanism of action of peroxisome proliferators in hepatocarcinogenesis. Carcinogenesis 21, 823–826.CrossRefGoogle Scholar
Richards, MP, Poch, SM, Coon, CN, Rosebrough, RW, Ashwell, CM, McMurtry, JP 2003. Feed restriction significantly alters lipogenic gene expression in broiler breeder chickens. Journal of Nutrition 133, 707–715.CrossRefGoogle ScholarPubMed
Rosa, G, Manco, M, Vega, N, Greco, AV, Castagneto, M, Vidal, H, Mingrone, G 2003. Decreased muscle acetyl-coenzyme a carboxylase 2 mRNA and insulin resistance in formerly obese subjects. Obesity Research 11, 1306–1312.CrossRefGoogle ScholarPubMed
Schneiter, R, Guerra, CE, Lampl, M, Tatzer, V, Zellnig, G, Klein, HL, Kohlwein, SD 2000. A novel cold-sensitive allele of the rate-limiting enzyme of fatty acid synthesis, acetyl coenzyme a carboxylase, affects the morphology of the yeast vacuole through acylation of Vac8p. Molecular and Cellular Biology 20, 2984–2995.CrossRefGoogle ScholarPubMed
Speake, BK, Murray, AM, Noble, RC 1998. Transport and transformations of yolk lipids during development of the avian embryo. Progress in Lipid Research 37, 1–32.CrossRefGoogle ScholarPubMed
Tontonoz, P, Hu, E, Graves, RA, Budavari, AI, Spiegelman, BM 1994. mPPARg2: tissue-specific regulator of an adipocyte enhancer. Genes and Development 8, 1224–1234.CrossRefGoogle ScholarPubMed
Tusher, VG, Tibshirani, R, Chu, G 2001. Significance analysis of microarrays applied to the ionizing radiation response. Proceedings of the National Academy of Sciences of the United States of America 98, 5116–5121.CrossRefGoogle Scholar
Volpe, JJ, Vagelos, PR 1973. Saturated fatty acid biosynthesis and its regulation. Annual Review of Biochemistry 42, 21–60.CrossRefGoogle ScholarPubMed
Wakil, SJ, Stoops, JK, Joshi, VC 1983. Fatty acid synthesis and its regulation. Annual Review of Biochemistry 52, 537–579.CrossRefGoogle ScholarPubMed
Wang, Q, Li, H, Leng, L, Wang, Y, Tang, Z, Li, N, Zhang, F 2007b. Polymorphism of heart fatty acid-binding protein gene associated with fatness traits in the chicken. Animal Biotechnology 18, 91–99.CrossRefGoogle ScholarPubMed
Wang, H, Li, H, Wang, Q, Wang, Y, Han, H, Shi, H 2006. Microarray analysis of adipose tissue gene expression profiles between two chicken breeds. Journal of Biosciences 31, 565–573.CrossRefGoogle ScholarPubMed
Wang, HB, Li, H, Wang, QG, Zhang, XY, Wang, SZ, Wang, YX, Wang, XP 2007a. Profiling of chicken adipose tissue gene expression by genome array. BMC Genomics 8, 193.CrossRefGoogle ScholarPubMed
Wolfe, RR, Peters, EJ 1987. Lipolytic response to glucose infusion in human subjects. American Journal of Physiology 252, E218–E223.Google ScholarPubMed
Zhang, H, Yang, Z, Shen, Y, Tong, L 2003. Crystal structure of the carboxyltransferase domain of acetyl-coenzyme a carboxylase. Science 299, 1683–1691.CrossRefGoogle ScholarPubMed