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Influence of damaging and wilting red clover on lipid metabolism during ensiling and in vitro rumen incubation

Published online by Cambridge University Press:  15 April 2010

G. Van Ranst ,
V. Fievez ,
M. Vandewalle ,
C. Van Waes ,
J. De Riek  and
E. Van Bockstaele
G. Van Ranst
Affiliation:
Unit Plant, Applied Genetics and Breeding, Institute for Agriculture and Fisheries Research, Caritasstraat 21, 9090 Melle, Belgium
V. Fievez*
Affiliation:
Department of Animal Production, Ghent University, LANUPRO, Proefhoevestraat 10, 9090 Melle, Belgium
M. Vandewalle
Affiliation:
Unit Plant, Applied Genetics and Breeding, Institute for Agriculture and Fisheries Research, Caritasstraat 21, 9090 Melle, Belgium
C. Van Waes
Affiliation:
Unit Plant, Crop Husbandry and Environment, Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 109, 9820 Merelbeke, Belgium
J. De Riek
Affiliation:
Unit Plant, Applied Genetics and Breeding, Institute for Agriculture and Fisheries Research, Caritasstraat 21, 9090 Melle, Belgium
E. Van Bockstaele
Affiliation:
Unit Plant, Applied Genetics and Breeding, Institute for Agriculture and Fisheries Research, Caritasstraat 21, 9090 Melle, Belgium Plant Production, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
*
†E-mail: Veerle.Fievez@UGent.be
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Abstract

This paper describes the relationship between protein-bound phenols in red clover, induced by different degrees of damaging before wilting and varying wilting duration, and in silo lipid metabolism. The ultimate effect of these changes on rumen biohydrogenation is the second focus of this paper. For this experiment, red clover, damaged to different degrees (not damaged (ND), crushing or frozen/thawing (FT)) before wilting (4 or 24 h) was ensiled. Different degrees of damaging and wilting duration lead to differences in polyphenol oxidase (PPO) activity, measured as increase in protein-bound phenols. Treatment effects on fatty acid (FA) content and composition, lipid fractions (free FAs, membrane lipids (ML) and neutral fraction) and lipolysis were further studied in the silage. In FT, red clover lipolysis was markedly lower in the first days after ensiling, but this largely disappeared after 60 days of ensiling, regardless of wilting duration. This suggests an inhibition of plant lipases in FT silages. After 60 days of ensiling no differences in lipid fractions could be found between any of the treatments and differences in lipolysis were caused by reduced FA proportions in ML of wilted FT red clover. Fresh, wilted (24 h) after damaging (ND or FT) and ensiled (4 or 60 days; wilted 24 h; ND or FT) red clover were also incubated in rumen fluid to study the biohydrogenation of C18:3n-3 and C18:2n-6 in vitro. Silages (both 60 days and to a lower degree 4 days) showed a lower biohydrogenation compared with fresh and wilted forages, regardless of damaging. This suggests that lipids in ensiled red clover were more protected, but this protection was not enhanced by a higher amount of protein-bound phenols in wilted FT compared with ND red clover. The reduction of rumen microbial biohydrogenation with duration of red clover ensiling seems in contrast to what is expected, namely a higher biohydrogenation when a higher amount of FFA is present. This merits further investigation in relation to strategies to activate PPO toward the embedding of lipids in phenol–protein complexes.

Keywords

polyphenol oxidasefatty acidPUFAbiohydrogenationlipolysis
Type
Full Paper
Information
animal , Volume 4 , Issue 9 , September 2010 , pp. 1528 - 1540
Copyright
Copyright © The Animal Consortium 2010

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