Organic cropping systems are characterized by soil-disturbance events that can be diversified over years through crop rotations and within seasons by varying planting dates. The Farming Systems Project at Beltsville, MD, USA, is a long-term experiment that includes three organic rotations, corn (Zea mays L.)–soybean [Glycine max (L.) Merr.], corn–soybean–wheat (Triticum aestivum L.), and corn–soybean–wheat–alfalfa (Medicago sativa L.). Analysis of weed presence and cover over the first 18 yr of this experiment revealed that the tall, erect annual broadleaf weeds smooth pigweed (Amaranthus hybridus L.), common lambsquarters (Chenopodium album L.), horseweed (Erigeron canadensis L.), jimsonweed (Datura stramonium L.), and/or velvetleaf (Abutilon theophrasti Medik.) were most prominent in corn and soybean. Generally, these species exhibited traits adapted to the disturbance regimes, nutrient availability, crop environment and duration, and local meteorological conditions associated with the summer annual corn and soybean crops. Abundance of A. hybridus, D. stramonium, and A. theophrasti were controlled primarily by rotation diversity, whereby presence and cover of these species were highest in the short corn–soybean rotation and lowest in the longer rotations that had more diverse seasonal soil-disturbance regimes. Early-season temperature was the primary factor controlling C. album presence and cover, which were higher at lower temperatures associated with earlier planting dates. Higher early-season precipitation was the primary factor associated with higher presence of annual grass species. The relative abundance of species in organic corn and soybean was determined primarily by the diversity of crops and disturbance operations in rotation, the timing of spring tillage and planting, and annual meteorological conditions driving emergence periodicity.

Organic crop production is often limited by the inability to control weeds. An 18-yr data set of weed cover in organic crop rotations at the long-term Farming Systems Project at Beltsville, MD, was analyzed to identify meteorological and management factors influencing weed abundance. A path analysis using structural equation models was employed to distinguish between the direct effect of factors on weed cover and the indirect effect on weed cover through effects on crop competitiveness. Grain yield of corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] served as a surrogate for crop competitiveness and was found to be the most important factor influencing weed cover. Precipitation during late vegetative and early reproductive crop growth had a strong positive effect on crop yield, and thereby a negative indirect effect on weed cover, but this effect was partially offset by a positive direct effect on weed cover. Delayed crop planting date and crop rotational diversification including crops other than summer row crops had a moderate negative effect on weed cover, while having minimal effect on crop performance. Rotary hoeing also had a direct negative effect on weed cover, but a corresponding negative effect on crop performance resulted in a diminished total effect on weeds. Results demonstrate the complex interactions that define the relative abundance of weeds faced by organic growers, but, generally, factors that enhanced crop competitiveness provided the most effective weed management.

Methyl isothiocyanate (MIT) consistently killed large crabgrass [Digitaria sanguinalis (L.) Scop. # DIGSA] seed at concentations of 4.0 mM or greater. Concentrations of 0.6 to 1.0 mM MIT delayed germination of large crabgrass seed but ultimately allowed the majority of seed to germinate. Dormant large crabgrass seed were killed at concentrations of MIT similar to those required to kill nondormant seed. MIT stimulated germination of dormant large crabgrass seed at sublethal concentrations (0.1 to 1.0 mM). Experiments with metham (sodium methyldithiocarbamate) in the greenhouse and field (metham rapidly degrades to MIT in soils) confirmed results of laboratory experiments with MIT.

Leaf tissue and leaf-tissue extracts from annual wormwood and pure artemisinin were evaluated for their effects on plant growth when incorporated into sandy loam soil. Dried leaf tissue was successively extracted with methylene chloride (MeCl2), ethanol (EtOH), and water, and the extracts and residue were reduced to dryness and stored at −20 C. Leaf tissue was incorporated in soil at rates equivalent to 0, 0.37, 0.73, or 1.1% (w/w) based on soil dry weight. Peat moss treated with extracts or artemisinin was incorporated into soil at a rate equivalent to the 0.73% (w/w) treatment. Inhibition of growth was species-specific; estimated reduction of dry weight by 0.73% (w/w) leaf tissue was 82, 49, 25, and 9% for redroot pigweed, common lambsquarters, soybean, and corn, respectively. The effects of the MeCl2 extract, which contained all of the extractable artemisinin, on germination and growth of redroot pigweed were similar to that of leaf tissue. Annual wormwood leaf tissue and MeCl2-extract treatments were the only treatments that resulted in a reduction in seedling survival. Artemisinin at levels equivalent to that contained in the MeCl2 extract and leaf-tissue treatments had significantly less effect on seedling survival, germination, and growth of redroot pigweed than the MeCl2 extract. Furthermore, the aqueous extract, which did not contain artemisinin, and the extract residue had activities similar to that of the artemisinin treatment. Thus, the allelopathic effects of annual wormwood can not be attributed to artemisinin alone.

A non-linear dose-response model was used to analyze the response of tomato (Lycopersicon esculentum Mill.) and eastern black nightshade (Solanum ptycanthum Dun. #3 SOLPT) to acifluorfen {5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid}, fluoroglycofen {carboxymethyl 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate}, fomesafen {5-[2-chloro-4-(trifluoromethyl)phenoxy]-N-(methylsulfonyl)-2-nitrobenzamide}, and lactofen {(±)-2-ethoxy-1-methyl-2-oxoethyl 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate}. The relative potency of these herbicides to both species was lactofen > fluoroglycofen > fomesafen > acifluorfen. Tomato was significantly more resistant to acifluorfen, fluoroglycofen, and lactofen than was eastern black nightshade, but there was no significant difference between species in response to fomesafen. At the rate required to reduce tomato weight by 10%, eastern black nightshade weight was reduced 48, 32, 20, and 5% by acifluorfen, fluoroglycofen, lactofen, and fomesafen, respectively.

The influence of various factors on the tolerance of corn and selected weed species to thifensulfuron was studied in greenhouse experiments. Corn fresh weight was reduced by postemergence application of thifensulfuron when applied at 180 g ai ha−1 without surfactant or at 18 g ha−1 with a nonionic surfactant. Corn was more susceptible to root exposure whereas velvetleaf was more susceptible to foliar exposure. Velvetleaf was most susceptible when plants were young, when a nonionic surfactant was added, and at 20 rather than 30 C. Simulated rainfall 8 h after application reduced velvetleaf injury by thifensulfuron at 18 g ha−1 without surfactant; however, with addition of a nonionic surfactant, velvetleaf injury was reduced only if rainfall occurred less than 2 h after application. Soil moisture level did not affect velvetleaf susceptibility.

Five field experiments were conducted with snap bean (Phaseolus vulgaris L.) row spacings of 15, 25, 36, 46, and 91 cm, all of which were seeded at a constant density of 43 plants/m2. When weeds were allowed to emerge with the crop, row spacings of 15 to 36 cm suppressed weed growth by 18% compared to the conventional 91-cm row spacing. When weeds were controlled for the first half of the season, row spacings of 15 to 36 cm suppressed weed growth by 82% compared to the 91-cm row spacing. The effect of the 46-cm row spacing on weed growth was variable. Weed suppression by narrow rows was explained by a higher rate of snap bean canopy closure in narrow rows. Snap beans in row spacings of 15 to 46 cm produced similar yields and were higher by an average of 23% than yields produced by snap beans in 91 - cm rows.

The influence of light, soil moisture, and temperature on establishment of selected species through hairy vetch residue on the soil surface was investigated under controlled conditions in the greenhouse. Hairy vetch residue at rates ranging from 0 to 616 g m−2 had no effect on corn, slightly reduced velvetleaf and green foxtail establishment, and severely inhibited common lambsquarters establishment under full sunlight conditions. The same rates of hairy vetch residue reduced velvetleaf, green foxtail, and common lambsquarters establishment more under a shade cloth with 9% light transmittance than under full sunlight. Day/night temperatures of 24/16 or 32/26 C had no effect and soil moistures of 50 or 133% field capacity had little effect on response of all species to residue rates. Weed establishment was similar under shade cloth without residue as under residue with an equivalent light transmittance, suggesting that light was more important than allelopathy or physical impedance for weed suppression by hairy vetch residue.

Hairy vetch was grown as a winter annual cover crop and evaluated for weed suppression when desiccated by paraquat or left alive until natural senescence in a 3-yr field experiment. Total weed density and biomass were variable in the desiccated hairy vetch treatment relative to a bare soil treatment but were consistently lower in the live hairy vetch treatment relative to the desiccated or bare soil treatments. An average of 87% of sites under live hairy vetch compared to 8% of sites under desiccated hairy vetch transmitted less than 1% of unobstructed sunlight. The red (660 nm) to far-red (730 nm) ratio of transmitted light was reduced by 70% under live hairy vetch compared to 17% under desiccated hairy vetch. Daily maximum soil temperature and diurnal soil temperature amplitude were reduced by live hairy vetch > desiccated hairy vetch > bare soil. Soil moisture content was greater under both live and desiccated hairy vetch compared to bare soil during droughty periods. Changes in light extinction, red to far-red ratio, and diurnal soil temperature amplitude were sufficient to explain greater weed suppression by live than desiccated hairy vetch.

Field experiments were conducted from 1989 to 1992 to determine whether no-tillage corn grown in 38-cm rows and a 2× population could improve weed control relative to 76-cm rows and 1× population under reduced-herbicide options. A standard treatment including 1.12 kg ai/ha of atrazine plus 2.24 kg ai/ha of metolachlor was compared with a treatment including the same herbicides applied at 25% of the standard rates. Both treatments included 0.56 kg ai/ha of paraquat which controlled annual weeds established at the time of application. Weed control was less in the 25%-herbicide treatment than in the standard treatment in two of four years when corn was grown in 76-cm rows with a l× population. The 25%-herbicide treatment provided weed control and grain yields similar to the standard treatment in each year when corn was grown in 38-cm rows with a 2× population. Weed control was poor and yield was reduced when no herbicides were applied regardless of row spacing or population. The leaf canopy of corn in the 38-cm row/2×-population treatment reduced light transmittance 1 wk earlier than corn in the 76-cm row/1×-population treatment.

In four field experiments, acifluorfen {5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid} applied preemergence at 1.1 kg/ha was not phytotoxic to peas (Pisum sativum L.) and increased the yield of shelled peas by an average of 25% compared to the weedy control. Yields were highest in all four experiments at the 1.1 kg/ha rate and tended to decline at higher rates of application. Growth chamber studies demonstrated that pea tolerance to acifluorfen was not affected by temperatures of 16/10 to 27/21C (light/dark) nor by stimulated rainfall of 0.5 to 5.0 cm. Peas and soybeans [Glycine max (L.) Merr.] were more tolerant of acifluorfen than were snap beans (Phaseolus vulgaris L.) and lima beans (Phaseolus lunatus L.) when acifluorfen was applied preemergence in the greenhouse or in hydroponic nutrient solution.

In three field experiments with weed-free plots, delaying the application of bentazon [3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide] reduced toxicity to cucumbers (Cucumis sativus L. ‘Poinsett 76′). Maximum injury and yield reduction were observed when bentazon was applied at the first leaf stage, whereas injury and yield reduction were slight when bentazon was applied at the fourth leaf stage. Increasing illuminance after application increased injury and tended to override the effect of leaf stage. In greenhouse studies, the slicing variety ‘Poinsett 76’ was more tolerant of bentazon than the pickling variety ‘Calypso’. The insecticide diazinon [O,O-diethyl-O-(2-isopropyl-6-methyl-4-pyrimidinyl)phosphorothioate] increased bentazon toxicity to cucumbers compared to toxicity caused by bentazon alone. Four other insecticides and two fungicides had no effect on phytotoxicity.

Weed management treatments with various degrees of herbicide inputs were applied with or without a hairy vetch cover crop to no-tillage corn in four field experiments at Beltsville, MD. A hairy vetch living mulch in the no-treatment control or a dead mulch in the mowed treatment improved weed control during the first 6 wk of the season but weed control deteriorated in these treatments thereafter. Competition from weeds and/or uncontrolled vetch in these treatments without herbicides reduced corn yield in three of four years by an average of 46% compared with a standard PRE herbicide treatment of 0.6 kg ai/ha of paraquat plus 1.1 kg ai/ha of atrazine plus 2.2 kg ai/ha of metolachlor. Reducing atrazine and metolachlor to one-fourth the rate of the standard treatment in the absence of cover crop reduced weed control in three of four years and corn yield in two of four years compared with the standard treatment. Hairy vetch had little influence on weed control or corn yield with any herbicide treatments.

We tested whether the efficacy of chemical weed control might change as atmospheric CO2 concentration [CO2] increases by determining if tolerance to a widely used, phloem mobile, postemergence herbicide, glyphosate, was altered by a doubling of [CO2]. Tolerance was determined by following the growth of Amaranthus retroflexus L. (redroot pigweed), a C4 species, and Chenopodium album L. (common lambsquarters), a C3 species, grown at near ambient (360 μmol mol−1) and twice ambient (720 μmol mol−1) [CO2] for 14 d following glyphosate application at rates of 0.00 (control), 0.112 kg ai ha−1 (0.1 X the commercial rate), and 1.12 kg ai ha−1 (1.0 X the commercial rate) in four separate trials. Irrespective of [CO2], growth of the C4 species, A. retroflexus, was significantly reduced and was eliminated altogether at glyphosate application rates of 0.112 and 1.12 kg ai ha−1, respectively However, in contrast to the ambient [CO2] treatment, an application rate of 0.112 kg ai ha−1 had no effect on growth, and a 1.12-kg ai ha−1 rate reduced but did not eliminate growth in elevated [CO2]-grown C. album. Although glyphosate tolerant does increase with plant size at the time of application, differences in glyphosate tolerance between CO2 treatments in C. album cannot be explained by size alone. These data indicate that rising atmospheric [CO2] could increase glyphosate tolerance in a C3 weedy species. Changes in herbicide tolerance at elevated [CO2] could limit chemical weed control efficacy and increase weed–crop competition.

Research was conducted to determine the optimum population and row spacing for corn production and for suppressing velvetleaf growth and seed production. Corn was grown in a factorial arrangement of three populations targeted at 64,000 (1 ×), 96,000 (1.5 ×), or 128,000 (2 ×) plants ha−1 and two row spacings of 38 or 76 cm. Influences on corn were determined in weed-free plots, and influences on velvetleaf were determined for target plants established at 1.5–m intervals along the center of corn interrows. Four velvetleaf plantings were made at weekly intervals beginning at corn planting. Corn row spacing had little influence on corn or velvetleaf. Corn yield exhibited a parabolic response to population with a maximum of approximately 90,000 plants ha−1 in one year, no response to population in another year, and a linear decline with increasing population in a dry year. Velvetleaf seed production was reduced 69 to 94% by the 1.5 × population and 99% by the 2 × population compared to the standard 1 × population when velvetleaf emerged with corn. Velvetleaf seed production was eliminated when velvetleaf emerged at or later than corn leaf stages 3, 5, and 6 for corn populations of 2 ×, 1.5 ×, and 1 ×, respectively. Reduced velvetleaf seed production was correlated with lower positioning of plants in the corn canopy and reduced light availability. Results suggest that higher corn populations could aid integrated weed management strategies by reducing seed production and limiting the build-up of weed populations.

Total weed density increased after 1 yr of no-tillage and after 2 yr of conventional tillage in a 4-yr experiment with repeated assignment of the same treatment to the same plots. Large crabgrass, goosegrass, and carpetweed densities were higher in the no-tillage compared with the conventional-tillage treatment in at least 1 yr whereas common lambsquarters density was greater in the conventional-tillage treatment the last year of the experiment. Within the no-tillage treatment, rye or hairy vetch residue reduced total weed density an average of 78% compared to the treatment without cover crop when cover crop biomass exceeded 300 g m–2 and when residue covered more than 90% of the soil. Goosegrass, stinkgrass, and carpetweed densities were reduced by cover crop residue in at least 1 yr whereas large crabgrass was unaffected. Common lambsquarters density increased where rye was grown as a cover crop prior to conventional tillage. Despite differences in weed density among treatments, weed biomass was equivalent in all treatments during the last 2 yr.

In greenhouse and growth-chamber studies, increasing the level of either light or temperature increased bentazon [3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide] phytotoxicity to cucumber (Cucumis sativus L.). Light had a greater influence on phytotoxicity than did temperature. Light and temperature conditions after bentazon application had a greater influence on phytotoxicity than did light and temperature conditions before application. Maximum phytotoxicity was obtained from treatment with low light level before application and high light level after application.

Increasing crop density is a cultural weed management practice that can compliment the use of cover crops for weed suppression. In this research, we created a range of cover crop biomass and soybean densities to assess their weed-suppressive ability alone and in combination. The experiment was conducted in 2008 and 2009 in Maryland and Pennsylvania using five levels of cereal rye residue, representing 0, 0.5, 1.0, 1.5, and 2.0 times the ambient level, and five soybean densities ranging from 0 to 74 seeds m−2. Weed biomass decreased with increasing rye residue and weeds were completely suppressed at levels above 1,500 g m−2. Weed biomass also decreased with increasing soybean density in 2 of 4 site–years. We evaluated weed suppression by fitting an exponential decay model of weed biomass as a function of rye biomass and a hyperbolic model of weed biomass as a function of soybean density at each of the five tactic levels. We multiplied these individual tactic models and included an interaction term to test for tactic interactions. In two of the four site-years, the combination of these tactics produced a synergistic interaction that resulted in greater weed suppression than would be predicted by the efficacy of each tactic alone. Our results indicate that increasing soybean planting rate can compensate for lower cereal rye biomass levels when these tactics are combined.

Insufficient weed control is a major constraint to adoption of reduced-tillage practices for organic grain production. Tillage, cover crop management, and crop planting date are factors that influence emergence periodicity and growth potential of important weed species in these systems. We assessed two hairy vetch cover crop management practices, disk-kill and roll-kill, across a range of corn planting dates from early May to late June in three experiments in Beltsville, MD. Patterns of seed dormancy, emergence, and early weed growth were determined for overseeded populations of common ragweed, giant foxtail, and smooth pigweed, three important species in the Mid-Atlantic states that represent early to late emergence. Common ragweed emergence was lowest and dormancy was highest of the three species across all planting dates. Giant foxtail emergence was higher than the other species in roll-killed hairy vetch and included a significant number of seeds that germinated before rolling operations in late June. Smooth pigweed had the highest emergence and lowest dormancy in disk-killed hairy vetch in June. Individual giant foxtail plant weight was higher in roll-killed than disk-killed hairy vetch in 2 of 3 yr, whereas that of smooth pigweed plants was higher in disk-killed than roll-killed vetch in 2 of 3 yr. Giant foxtail was the dominant species in roll-killed hairy vetch (averaged 79% of total weed biomass at corn silking), probably because of early germination and establishment before rolling operations. Smooth pigweed was the dominant species in disk-killed hairy vetch at June planting dates (averaged 77% of total weed biomass), probably because of high growth rates under warm conditions in tilled soil. This research demonstrated that cover crop management practices and the timing of planting operations can shift the dominant species of weed communities in organic farming systems and must be considered in long-term weed management planning.

Mulches on the soil surface are known to suppress weed emergence, but the quantitative relationships between emergence and mulch properties have not been clearly defined. A theoretical framework for describing the relationships among mulch mass, area index, height, cover, light extinction, and weed emergence is introduced. This theory is applied to data from experiments on emergence of four annual weed species through mulches of selected materials applied at six rates. Mulch materials, in order from lowest to highest surface-area-to-mass ratio, were bark chips, Zea mays stalks, Secale cereale, Trifolium incarnatum, Vicia villosa, Quercus leaves, and landscape fabric strips. The order of weed species' sensitivity to mulches was Amaranthus retroflexus > Chenopodium album > Setaria faberi > Abutilon theophrasti, regardless of mulch material. The success of emergence through mulches was related to the capacity of seedlings to grow around obstructing mulch elements under limiting light conditions. Mulch area index was a pivotal property for quantitatively defining mulch properties and understanding weed emergence through mulches. A two-parameter model of emergence as a function of mulch area index and fraction of mulch volume that was solid reasonably predicted emergence across the range of mulches investigated.