Female fertility is a complex trait with age-specific changes in spontaneous dizygotic (DZ) twinning and fertility. To elucidate factors regulating female fertility and infertility, we conducted a genome-wide association study (GWAS) on mothers of spontaneous DZ twins (MoDZT) versus controls (3273 cases, 24,009 controls). This is a follow-up study to the Australia/New Zealand (ANZ) component of that previously reported (Mbarek et al., 2016), with a sample size almost twice that of the entire discovery sample meta-analysed in the previous article (and five times the ANZ contribution to that), resulting from newly available additional genotyping and representing a significant increase in power. We compare analyses with and without male controls and show unequivocally that it is better to include male controls who have been screened for recent family history, than to use only female controls. Results from the SNP based GWAS identified four genomewide significant signals, including one novel region, ZFPM1 (Zinc Finger Protein, FOG Family Member 1), on chromosome 16. Previous signals near FSHB (Follicle Stimulating Hormone beta subunit) and SMAD3 (SMAD Family Member 3) were also replicated (Mbarek et al., 2016). We also ran the GWAS with a dominance model that identified a further locus ADRB2 on chr 5. These results have been contributed to the International Twinning Genetics Consortium for inclusion in the next GWAS meta-analysis (Mbarek et al., in press).

Cyclosporiasis results from an infection of the small intestine by Cyclospora parasites after ingestion of contaminated food or water, often leading to gastrointestinal distress. Recent developments in temporally linking genetically related Cyclospora isolates demonstrated effectiveness in supporting epidemiological investigations. We used ‘temporal-genetic clusters’ (TGCs) to investigate reported cyclosporiasis cases in the United States during the 2021 peak-period (1 May – 31 August 2021). Our approach split 655 genotyped isolates into 55 genetic clusters and 31 TGCs. We linked two large multi-state epidemiological clusters (Epidemiologic Cluster 1 [n = 136 cases, 54 genotyped] and Epidemiologic Cluster 2 [n = 42 cases, 15 genotyped]) to consumption of lettuce varieties; however, product traceback did not identify a specific product for either cluster due to the lack of detailed product information. To evaluate the utility of TGCs, we performed a retrospective case study comparing investigation outcomes of outbreaks first detected using epidemiological methods with those of the same outbreaks had TGCs been used to first detect them. Our study results indicate that adjustments to routine epidemiological approaches could link additional cases to epidemiological clusters of cyclosporiasis. Overall, we show that CDC’s integrated genotyping and epidemiological investigations provide valuable insights into cyclosporiasis outbreaks in the United States.

The burial of multiple individuals within a single funerary monument invites speculation about the relationships between the deceased: were they chosen on the basis of status, gender or relatedness, for example? Here, the authors present the results of aDNA and isotope analyses conducted on seven individuals from an Early Iron Age barrow at Dolge njive, south-eastern Slovenia. All seven individuals are close biological relatives. While the group composition suggests strict adherence to neither patrilineal nor matrilineal structures, the funerary tradition appears highly gendered, and family links through both the male and female lines seem important in structuring of the community. The results have implications for understanding of kinship and funerary practices in late prehistoric Europe.

Coexistence of people and large carnivores depends on a complex combination of factors that vary geographically. Both the number and range of the Asiatic lion Panthera leo leo in the Greater Gir landscape, India, has increased since the 1990s. The challenge has been managing the success of conservation, with a particular focus on the spillover population ranging extensively in human-dominated landscapes. To understand the factors conducive to lion survival in this landscape, we undertook an interview-based survey. Overall, people expressed positive, tolerant attitudes towards lions. There was a distinct contrast between people's liking for lions (76.9% of respondents) compared to leopards (27.7%) in spite of greater depredation of livestock by lions (82.6%) than by leopards (17.4%). Younger people and respondents having greater awareness regarding lions expressed positive attitudes. Although community discussions on lions had a positive effect, there was no evidence that land-holding, management interventions, personal encounters with lions, or association of lions with religion affected attitudes. Respondents who had experienced livestock depredation tended to express negative attitudes. Respondents with positive attitudes towards lions favoured non-interventionist strategies for managing lions in the village areas. We advocate consideration of varied factors influencing tolerance of wildlife in conservation planning. We emphasize that site-specific human–wildlife conflict issues such as crop-foraging by wild ungulates and variation in attitudes towards different species should also be considered. Specifically, improved livestock management, motivation of local youth and their participation in awareness campaigns could all further strengthen the prevalent positive attitudes towards lions.

Whether monozygotic (MZ) and dizygotic (DZ) twins differ from each other in a variety of phenotypes is important for genetic twin modeling and for inferences made from twin studies in general. We analyzed whether there were differences in individual, maternal and paternal education between MZ and DZ twins in a large pooled dataset. Information was gathered on individual education for 218,362 adult twins from 27 twin cohorts (53% females; 39% MZ twins), and on maternal and paternal education for 147,315 and 143,056 twins respectively, from 28 twin cohorts (52% females; 38% MZ twins). Together, we had information on individual or parental education from 42 twin cohorts representing 19 countries. The original education classifications were transformed to education years and analyzed using linear regression models. Overall, MZ males had 0.26 (95% CI [0.21, 0.31]) years and MZ females 0.17 (95% CI [0.12, 0.21]) years longer education than DZ twins. The zygosity difference became smaller in more recent birth cohorts for both males and females. Parental education was somewhat longer for fathers of DZ twins in cohorts born in 1990–1999 (0.16 years, 95% CI [0.08, 0.25]) and 2000 or later (0.11 years, 95% CI [0.00, 0.22]), compared with fathers of MZ twins. The results show that the years of both individual and parental education are largely similar in MZ and DZ twins. We suggest that the socio-economic differences between MZ and DZ twins are so small that inferences based upon genetic modeling of twin data are not affected.

The long-term stability of mechanically exfoliated MoS2 flakes was compared for storage in the air and storage under vacuum. Significant changes in MoS2 flakes were observed for samples stored in the air, whereas similar flakes on samples stored in vacuum underwent no change. Small speckles were observed to appear on the surface of flakes stored in the air, followed by thinning and eventual decomposition of MoS2 flakes. The speckles are suspected to be formed by oxidation of MoS2 in the presence of atmospheric oxygen and water molecules, resulting in the formation of hydrated MoO3.

Scholars are increasingly utilizing online workforces to encode latent political concepts embedded in written or spoken records. In this letter, we build on past efforts by developing and validating a crowdsourced pairwise comparison framework for encoding political texts that combines the human ability to understand natural language with the ability of computers to aggregate data into reliable measures while ameliorating concerns about the biases and unreliability of non-expert human coders. We validate the method with advertisements for U.S. Senate candidates and with State Department reports on human rights. The framework we present is very general, and we provide free software to help applied researchers interact easily with online workforces to extract meaningful measures from texts.

A major hindrance to establishment of successful complementary forage systems that include warm-season perennial grasses and clovers is tolerance of the latter to herbicides available for weed control. Field experiments were conducted in 2013 at two locations in northeast Louisiana to evaluate simulated residual rate effects of fluroxypyr plus triclopyr and 2,4-D plus picloram applied at 0, 0.25, 0.38, and 0.5× use rates immediately after fall planting of ball, white, crimson, and red clover. For all clovers, when averaged across herbicide rates, plant population 161/171 d after planting (DAP), ground cover, and height 184/196 DAP were equivalent for fluroxypyr plus triclopyr and the nontreated control and greater than 2,4-D plus picloram. Averaged across clovers, plant height after all rates of fluroxypyr plus triclopyr was equivalent to the nontreated control (14.2 to 14.3 vs. 15.3 cm) and greater than 2,4-D plus picloram. Compared with the nontreated control, 2,4-D plus picloram at 25, 38, and 50% of the normal use rates reduced height 58, 76, and 85%, respectively. When averaged across clover species, yield for fluroxypyr plus triclopyr at all rates was equivalent to the nontreated control (2,624 to 2,840 vs. 2,812 kg ha−1). Compared with the nontreated control, 2,4-D plus picloram at the 0.25, 0.38, and 0.50× use rates reduced yield 65, 89, and 99%, respectively.

Analysis of climatic and topographic evidence from the Cascade Range of Washington State indicates that glacial erosion limits the height and controls the morphology of this range. Glacial erosion linked to long-term spatial gradients in the ELA created a tilted, planar zone of 373 cirques across the central part of the range; peaks and ridges now rise ≤600 m above this zone. Hypsometric analysis of the region shows that the proportion of land area above the cirques drops sharply, and mean slopes >30° indicate that the areas above the cirques may be at or near threshold steepness. The mean plus 1σ relief of individual cirque basins (570 m) corresponds to the ∼600-m envelope above which peaks rarely rise. The summit altitudes are set by a combination of higher rates of glacial and paraglacial erosion above the ELA and enhanced hillslope processes due to the creation of steep topography. On the high-precipitation western flank of the Cascades, the dominance of glacial and hillslope erosion at altitudes at and above the ELA may explain the lack of a correspondence between stream-power erosion models and measured exhumation rates from apatite (U-Th/He) thermochronometry.

Field surveys and radiocarbon dating of buried logjams in the floodplain of an old-growth forest river demonstrate the formation of erosion-resistant “hard points” on the floodplain of the Queets River, Washington. These hard points provide refugia for development of old-growth forest patches in frequently disturbed riparian environments dominated by immature forest. Our surveys show that local bed aggradation associated with logjams not only influences channel patterns and profiles but leads to development of a patchwork of elevated landforms that can coalesce to form portions of the valley bottom with substantial (i.e., 1 to > 4 m) relief above the bankfull elevation. In addition, logjam-formed hard points promote channel avulsion, anastomosing morphology, and growth of mature patches of floodplain forest that, in turn, provide large logs needed to form more logjam-formed hard points. Hence, our findings substantiate the potential for a feedback mechanism through which hard points sustain complex channel morphology and a patchwork floodplain composed of variable-elevation surfaces. Conversely, such a feedback further implies that major changes in riparian forest characteristics associated with land use can lead to dramatic simplification in channel and floodplain morphology.

Geomorphic, stratigraphic, geotechnical, and biogeographic evidence indicate that failure of a Pleistocene ice dam between 15.5 and 26 ka generated a megaflood from Glacial Lake Atna down the Matanuska Valley. While it has long been recognized that Lake Atna occupied ⩾9000 km2 of south-central Alaska's Copper River Basin, little attention has focused on the lake's discharge locations and behaviors. Digital elevation model and geomorphic analyses suggest that progressive lowering of the lake level by decanting over spillways exposed during glacial retreat led to sequential discharges down the Matanuska, Susitna, Tok, and Copper river valleys. Lake Atna's size, ∽50 ka duration, and sequential connection to four major drainages likely made it a regionally important late Pleistocene freshwater refugium. We estimate a catastrophic Matanuska megaflood would have released 500–1400 km3 at a maximum rate of ⩾3×106 m3 s–1. Volumes for the other outlets ranged from 200 to 2600 km3 and estimated maximum discharges ranged from 0.8 to 11.3×106 m3 s–1, making Lake Atna a serial generator of some of the largest known freshwater megafloods.

Lacustrine and alluvial terraces and sediments record the extent of at least two Holocene glacially dammed lakes immediately upstream of the Tsangpo River gorge at the eastern syntaxis of the Himalaya. The larger lake covered 2835 km2, with a maximum depth of 680 m and contained an estimated 832 km3 of water; the smaller lake contained an estimated 80 km3 of water. Radiocarbon dating of wood and charcoal yielded conventional radiocarbon ages of 8860 ± 40 and 9870 ± 50 14C yr B.P. for the higher set of lake terraces, and 1220 ± 40 and 1660 ± 40 14C yr B.P. for sediments from the lower terraces. Catastrophic failure of the glacial dams that impounded the lakes would have released outburst floods down the gorge of the Tsangpo River with estimated peak discharges of up to 1 to 5 X 106 m3 s–1. The erosive potential represented by the unit stream power calculated for the head of the gorge during such a catastrophic lake breakout indicates that post-glacial megafloods down the Tsangpo River were likely among the most erosive events in recent Earth history.

Three sites on the Point Reyes Peninsula, California, provide evidence of major environmental change during the Pleistocene-Holocene transition. A 12,300-yr-long pollen record from Coast Trail Pond reveals a change from closed canopy Pseudotsuga-Abies forest to coastal scrub and grassland about 10,000 yr B.P. The same change is also evident in pollen records from two sea cliff exposures that contain a series of buried soils interstratified with fluvial and debris-flow deposits. Eight radiocarbon dates from these exposures indicate that aggradation at both sites began ca. 12,000 yr B.P. and that the change from forest to scrub and grassland occurred between 10,300 and 9400 yr B.P. High percentages of alder pollen and bracken fern spores reflect repeated geomorphic disturbance at ca. 10,400 yr B.P. The occurrence of major aggradation accompanied by recurrent debris flows between ca. 12,000 and 10,000 yr B.P. suggests that this was a period of more frequent high-intensity storms.

Reservoir sedimentation data and sediment yields from Taiwanese rivers show increased soil erosion in response to both 20th century changes in land use and a more recent increase in typhoon frequency and intensity. Decadal variations of up to 5- to 20-fold in suspended-sediment rating curves demonstrate supply-limited transport and correspond to increased sediment delivery from hillslopes due to changes in land use, regional ground shaking during the Chi-Chi earthquake, and post-2000 changes in typhoon frequency and intensity. While accelerated erosion in central Taiwan after the Chi-Chi earthquake has been documented previously, our results show that periods of increased upland erosion also occurred earlier, in response to 20th century changes in land use. Analyses of rainfall records and typhoon frequency for the period 1900–2009 further point to an island-wide increase in erosion rates corresponding to increased typhoon frequency and intensity after 1990.

An assemblage of fossil sockeye salmon was discovered in Pleistocene lake sediments along the South Fork Skokomish River, Olympic Peninsula, Washington. The fossils were abundant near the head of a former glacial lake at 115 m elevation. Large adult salmon are concentrated in a sequence of death assemblages that include individuals with enlarged breeding teeth and worn caudal fins indicating migration, nest digging, and spawning prior to death. The specimens were 4 yr old and 45–70 cm in total length, similar in size to modern sockeye salmon, not landlocked kokanee. The fossils possess most of the characteristics of sockeye salmon, Oncorhynchus nerka, but with several minor traits suggestive of pink salmon, O. gorbuscha. This suggests the degree of divergence of these species at about 1 million yr ago, when geological evidence indicates the salmon were deposited at the head of a proglacial lake impounded by the Salmon Springs advance of the Puget lobe ice sheet. Surficial geology and topography record a complicated history of glacial damming and river diversion that implies incision of the modern gorge of the South Fork Skokomish River after deposition of the fossil-bearing sediments.

In this article, we challenge the conclusion that the preferences of members of Congress are best represented as existing in a low-dimensional space. We conduct Monte Carlo simulations altering assumptions regarding the dimensionality and distribution of member preferences and scale the resulting roll call matrices. Our simulations show that party polarization generates misleading evidence in favor of low dimensionality. This suggests that the increasing levels of party polarization in recent Congresses may have produced false evidence in favor of a low-dimensional policy space. However, we show that focusing more narrowly on each party caucus in isolation can help researchers discern the true dimensionality of the policy space in the context of significant party polarization. We re-examine the historical roll call record and find evidence suggesting that the low dimensionality of the contemporary Congress may reflect party polarization rather than changes in the dimensionality of policy conflict.