Book contents
- Frontmatter
- Contents
- Foreword by Andrew Fire
- Foreword by Marshall Nirenberg
- List of Contributors
- Introduction
- Section one Basic RNAi, siRNA, microRNAs and gene-silencing mechanisms
- Section two Design, synthesis of siRNAs
- Section three Vector development and in vivo, in vitro and in ovo delivery methods
- 10 Six methods of inducing RNAi in mammalian cells
- 11 Viral delivery of shRNA
- 12 siRNA delivery by lentiviral vectors: Design and applications
- 13 Liposomal delivery of siRNAs in mice
- 14 Chemical modifications to achieve increased stability and sensitive detection of siRNA
- 15 RNA interference in postimplantation mouse embryos
- 16 In ovo RNAi opens new possibilities for functional genomics in vertebrates
- Section four Gene silencing in model organisms
- Section five Drug target validation
- Section six Therapeutic and drug development
- Section seven High-throughput genome-wide RNAi analysis
- Index
- Plate section
- References
15 - RNA interference in postimplantation mouse embryos
Published online by Cambridge University Press: 31 July 2009
Edited by
Book contents
- Frontmatter
- Contents
- Foreword by Andrew Fire
- Foreword by Marshall Nirenberg
- List of Contributors
- Introduction
- Section one Basic RNAi, siRNA, microRNAs and gene-silencing mechanisms
- Section two Design, synthesis of siRNAs
- Section three Vector development and in vivo, in vitro and in ovo delivery methods
- 10 Six methods of inducing RNAi in mammalian cells
- 11 Viral delivery of shRNA
- 12 siRNA delivery by lentiviral vectors: Design and applications
- 13 Liposomal delivery of siRNAs in mice
- 14 Chemical modifications to achieve increased stability and sensitive detection of siRNA
- 15 RNA interference in postimplantation mouse embryos
- 16 In ovo RNAi opens new possibilities for functional genomics in vertebrates
- Section four Gene silencing in model organisms
- Section five Drug target validation
- Section six Therapeutic and drug development
- Section seven High-throughput genome-wide RNAi analysis
- Index
- Plate section
- References
Summary
Introduction
Sequencing of whole genomes has provided new perspectives into the blueprints of diverse organisms, including the genome of the mouse (Waterston et al., 2002). Although the complete sequence is now available, the estimation of total gene number encoded by the mouse genome is ranging approximately from 25,000 to 50,000 (Okazaki et al., 2002). This uncertainty about the functional units within the genome highlights the importance of a detailed analysis of the encoded genes.
A significant step toward a better understanding of the genome has been the development of large-scale gene expression analysis tools utilizing DNA microarrays (Bono et al., 2003). This technology allows the generation of gene expression profiles that can give important clues for the interpretation of biological processes. However, the obtained data do not directly address the function of individual genes. Rather, they present a snapshot of global gene expression changes. While this is a very useful parameter for understanding the genome, it is not very useful for studying detailed phenotypic changes after gene ablation.
About 15 years ago gene function analysis became available in the mouse through the development of gene knock-out technology (Capecchi, 1989). In this approach genes are targeted in embryonic stem (ES) cells through homologous recombination. The manipulated ES cells are subsequently injected into blastocysts, and chimeric offspring are checked for germline transmission. Successful germline transmission allows the production of animals deficient in the gene of interest. Careful phenotypic analyses of these animals can then disclose the function(s) of the knocked-out gene.
- Type
- Chapter
- Information
- RNA Interference TechnologyFrom Basic Science to Drug Development, pp. 207 - 219Publisher: Cambridge University PressPrint publication year: 2005
References
, , , , , , , , and (1999). Mammalian ELAV-like neuronal RNA-binding proteins HuB and HuC promote neuronal development in both the central and the peripheral nervous systems. Proceedings of the National Academy of Sciences USA, 96, 9885–9890CrossRefGoogle ScholarPubMed
and (1998). Assessing the potential of skin electroporation for the delivery of protein- and gene-based drugs. Trends in Biotechnology, 16, 408–412CrossRefGoogle ScholarPubMed
(1998). Reflections on the culture of the preimplantation embryo. International Journal of Developmental Biology, 42, 879–884Google ScholarPubMed
, , , , , , , , , et al. (2003). Systematic expression profiling of the mouse transcriptome using RIKEN cDNA microarrays. Genome Research, 13, 1318–1323CrossRefGoogle ScholarPubMed
, , , and (2002). Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA. Proceedings of the National Academy of Sciences USA, 99, 14236–14240CrossRefGoogle ScholarPubMed
and (2003). An inhibition of cyclinlin-002D;dependent kinases that lengthens, but does not arrest, neuroepithelial cell cycle induces premature neurogenesis. Journal of Cell Science, 116, 4947–4955CrossRefGoogle Scholar
, , , and (2004). Tissue-specific RNA interference in post-implantation mouse embryos using directional electroporation and whole embryo culture. Differentiation, 72, 92–102CrossRefGoogle ScholarPubMed
(1989). Altering the genome by homologous recombination. Science, 244, 1288–1292CrossRefGoogle ScholarPubMed
Cockroft, D. L. (1990). Dissection and culture of postimplantation embryos. In Postimplantatio mammalian embryos. A practical approach (ed. Rickwood, D. and Hames, B. D.), pp. 15–40. Oxford: Oxford University Press
and (2001). The influence of growth factors on the development of preimplantation mammalian embryos. Archives of Medical Research, 32, 619–626CrossRefGoogle ScholarPubMed
and (1991). Integrins play an essential role in somite adhesion to the embryonic axis. Developmental Biology, 143, 418–421CrossRefGoogle ScholarPubMed
, , , , and (2001a). Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature, 411, 494–498CrossRefGoogle Scholar
, and (2001b). RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes & Development, 15, 188–200CrossRefGoogle Scholar
, and (2003). RNA inhibition of BMP-4 gene expression in postimplantation mouse embryos. Genetics, 37, 12–17Google ScholarPubMed
, , and (2002). Small interfering RNA and gene silencing in transgenic mice and rats. FEBS Letters, 532, 227–230CrossRefGoogle ScholarPubMed
, , and (2004). Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis. Proceedings of the National Academy of Science USA, 101, 3196–3201CrossRefGoogle Scholar
(1890). Preliminary note on the transplantation and growth of mammalian ova within a uterine foster-mother. Proceedings of the Royal Society of London, B. Biological Sciences, 48, 457–459CrossRefGoogle Scholar
Henschel, A., Buchholz, F. and Habermann, B. (2004). DEQOR: a web-based tool for the design and quality control of siRNAs. Nucleic Acids Res. 2004 Jul 1; 32 (Web Server issue): W113–20CrossRef
, , , , and (1999). Expression of the antiproliferative gene TIS21 at the onset of neurogenesis identifies single neuroepithelial cells that switch from proliferative to neuron-generating division. Proceedings of the National Academy of Sciences USA, 96, 4639–4644CrossRefGoogle ScholarPubMed
, and (2000). Fate mapping of the mouse prosencephalic neural plate. Developmental Biology, 219, 373–383CrossRefGoogle ScholarPubMed
, , and (2003). siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells. Nucleic Acids Research, 31, 981–987CrossRefGoogle ScholarPubMed
and (2003). RNA interference: Gene silencing in the fast lane. Seminars in Cancer Biology, 13, 259–265CrossRefGoogle ScholarPubMed
and (2003). Conditional knockout mice. Methods in Molecular Biology, 209, 159–185Google ScholarPubMed
, , , , and (2003). Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype. Nature Biotechnology, 21, 559–561CrossRefGoogle ScholarPubMed
(2001). Conditional control of gene expression in the mouse. Nature Reviews Genetics, 2, 743–755CrossRefGoogle ScholarPubMed
, , , and (2002). Efficient delivery of siRNA for inhibition of gene expression in postnatal mice. Nature Genetics, 32, 107–108CrossRefGoogle ScholarPubMed
, , and (2002). Spatial and temporal ‘knock down’ of gene expression by electroporation of double-stranded RNA and morpholinos into early postimplantation mouse embryos. Mechanisms of Development, 118, 57–63CrossRefGoogle ScholarPubMed
, , and (2003). Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing. Nature Biotechnology, 21, 324–328CrossRefGoogle ScholarPubMed
Nagy, A., Gertsenstein, M., Vintersten, K. and Behringer, R. R. (2003). In Manipulating the mouse embryo. A laboratory manual (eds. J. Inglis and J. Cuddihy), pp. 209–250. New York: Cold Spring Harbor Laboratory Press
(1978). Whole-embryo culture and the study of mammalian embryos during embryogenesis. Biological Reviews, 53, 81–122CrossRefGoogle Scholar
Nicholas, J. S. and Rudnick, D. (1938). The development of rat embryos in tissue culture. Proceedings of the National Academy of Sciences USA, 656–658
Oback, B., Cid-Arregui, A. and Huttner, W. B. (2000). Gene transfer into cultured postimplantation mouse embryos using herpes simplex amplicons. In Viral Vectors: Basic Science and Gene Therapy, (ed. A. C. -A. A. García-Carrancá), pp. 277–293. Natick, MA: Eaton Publishing
et al. (2002). Analysis of the mouse transcriptome based on functional annotation of 60770 full-length cDNAs. Nature, 420, 563–573CrossRefGoogle ScholarPubMed
and (2001). Gene transfer into cultured mammalian embryos by electroporation. Methods, 24, 35–42CrossRefGoogle ScholarPubMed
and (2001). Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Developmental Biology, 240, 237–246CrossRefGoogle ScholarPubMed
, , and (1984). Introduction of a selectable gene into different animal tissue by a retrovirus recombinant vector. Proceedings of the National Academy of Sciences USA, 81, 7151–7155CrossRefGoogle ScholarPubMed
, , and (2001). Sparking new frontiers: using in vivo electroporation for genetic manipulations. Developmental Biology, 233, 13–21CrossRefGoogle ScholarPubMed
and (2001). Efficient in utero gene transfer system to the developing mouse brain using electroporation: Visualization of neuronal migration in the developing cortex. Neuroscience, 103, 865–872CrossRefGoogle ScholarPubMed
, , and (2002). Manipulating gene expressions by electroporation in the developing brain of mammalian embryos. Differentiation, 70, 155–162CrossRefGoogle ScholarPubMed
(1998). Postimplantation mouse development: Whole embryo culture and micro-manipulation. International Journal of Developmental Biology, 42, 895–902Google ScholarPubMed
et al. (2002). Initial sequencing and comparative analysis of the mouse genome. Nature, 420, 520–562Google ScholarPubMed
, and (1997). A review of the contribution of whole embryo culture to the determination of hazard and risk in teratogenicity testing. International Journal of Developmental Biology, 41, 329–335Google ScholarPubMed
, , , , , and (2002). Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells. Proceedings of the National Academy of Sciences USA, 99, 9942–9947CrossRefGoogle ScholarPubMed