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11 - Ex situ conservation of ferns and lycophytes – approaches and techniques

Published online by Cambridge University Press:  11 August 2009

Valerie C. Pence
Affiliation:
Plant Conservation Division, Center for Research of Endangered Wildlife, Cincinnati Zoo and Botanic Garden, Cincinnati, OH 45220, USA
Tom A. Ranker
Affiliation:
University of Colorado, Boulder
Christopher H. Haufler
Affiliation:
University of Kansas
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Summary

Introduction

Although generally less conspicuous than seed plants, ferns and lycophytes have been the object of human interest since at least the time of the ancient Greeks. As with other plant and animal species, habitat loss is the major threat, but harvesting for medicine, food, or as ornamental plants has also depleted some species in the wild. Of the approximately 13000 named species, approximately 800 are of conservation concern (Walter and Gillett, 1998). However, some areas of the world are still poorly explored for ferns and lycophytes, and although the exact number of endangered species is uncertain, many species are in habitats that are under pressure. While conserving species in situ is the ideal, it cannot always be ensured. As a result, ex situ conservation methods can play a complementary role in ensuring the survival of these species, by providing a back-up of genetic diversity for the populations in the wild.

Growing ferns and lycophytes ex situ as horticultural or botanically interesting specimens has a long history, but other ex situ conservation methods, such as spore banking and cryopreservation are more recent. Whereas spore banking follows protocols similar to those of seed banking, the independent alternation of generations in ferns and lycophytes provides opportunities for cryopreservation that are not available in seed plants. Previous reviews have described some of these possibilities (Page et al., 1992; Pattison et al., 1992; Pence, 2002).

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Publisher: Cambridge University Press
Print publication year: 2008

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References

Agrawal, D. C., Pawar, S. S., and Mascarenhas, A. F. (1993). Cryopreservation of spores of Cyathea spinulosa Wall. ex. Hook. f.: an endangered tree fern. Journal of Plant Physiology, 142, 124–126.CrossRefGoogle Scholar
Allsopp, A. (1952). Longevity of Marsilea sporocarps. Nature, 169, 79–80.CrossRefGoogle Scholar
Ambrozic-Dolinsek, J., Camloh, M., Bohanec, B. and Zel, J. (2002). Apospory in leaf culture of staghorn fern (Platycerium bifurcatum). Plant Cell Reports, 20, 791–796.Google Scholar
Aragón, C. F. and Pangua, E. (2004). Spore viability under different storage conditions in four rupicolous Asplenium L. taxa. American Fern Journal, 94, 28–38.CrossRefGoogle Scholar
Ballesteros, D. and Walters, C. (2007). Water properties in fern spores: sorption characteristics relating to water affinity, glassy states, and storage ability. Journal of Experimental Botany, 58, 1185–1196.CrossRefGoogle Scholar
Ballesteros, D., Ibars, A. M., and Estrelles, E. (2004). New data about pteridophytic spore conservation in germplasm banks. Planta Europa IVth Conference, Valencia, Spain. www.nerium.net/plantaeuropa/Download/Procedings/Ballesteros_ daniel.pdf.
Ballesteros, D., Estrelles, E., and Ibars, A. M. (2006). Responses of pteridophyte spores to ultrafreezing temperatures for long-term conservation in germplasm banks. The Fern Gazette, 17, 293–302.Google Scholar
Beri, A. and Bir, S. S. (1993). Germination of stored spores of Pteris vittata L. American Fern Journal, 83, 73–78.CrossRefGoogle Scholar
Calderon-Saenz, E. (2000). Production of adventitious buds on the leaves in Dicksonia sellowiana. American Fern Journal, 90, 105–108.CrossRefGoogle Scholar
Camloh, M. (1999). Spore age and sterilization affects germination and early gametophyte development of Platycerium bifurcatum. American Fern Journal, 89, 124–132.CrossRefGoogle Scholar
Camloh, M., Vilhar, B., Zel, J., and Ravnikar, M. (1999). Jasmonic acid stimulates development of rhizoids and shoots in fern leaf culture. Journal of Plant Physiology, 155, 798–801.CrossRefGoogle Scholar
Capellades Queralt, M., Beruto, M., Vanderschaeghe, A., and DeBergh, P. C. (1991). Ornamentals. In Micropropagation Technology and Application, ed. Debergh, P. C. and Zimmerman, R. H.. Dordrecht: Kluwer, pp. 215–229.CrossRefGoogle Scholar
Castle, H. (1953). Notes on the development of the gametophytes of Equisetum arvense in sterile media. Botanical Gazette, 114, 323–328.CrossRefGoogle Scholar
Chá-Chá, R., Fernandes, F., and Romano, A. (2003). Preservation of Polystichum drepanum (Sw.) C. Presl. an endemic pteridophyte of Madeira Island. Revista de Biologia, 21, 7–16.Google Scholar
Dyer, A. F. (1979). The culture of fern gametophytes for experimental investigation. In The Experimental Biology of Ferns, ed. Dyer, A. F.. London: Academic Press, pp. 253–305.Google Scholar
Dyer, A. F. (1994). Natural soil spore banks. Can they be used to retrieve lost ferns? Biodiversity and Conservation, 3, 160–175.CrossRefGoogle Scholar
Dyer, A. F. and Lindsay, S. (1996). Soil spore banks – a new resource for conservation. In Pteridology in Perspective, ed. Camus, J. M., Gibby, M., and Johns, R. J.. Kew: Royal Botanic Gardens, pp. 153–160.Google Scholar
Engelmann, F. and Takagi, H. (2000). Cryopreservation of Tropical Plant Germplasm. Rome: International Plant Genetic Resources Institute.Google Scholar
Fabre, J. and Dereuddre, J. (1990). Encapsulation-dehydration: a new approach to cryopreservation of Solanum shoot tips. Cryoletters, 11, 413–426.Google Scholar
Fay, M. F. (1992). Conservation of rare and endangered plants using in vitro methods. In vitro Cellular and Developmental Biology – Plant, 28, 1–4.CrossRefGoogle Scholar
Fernández, H. and Revilla, M. A. (2003). In vitro culture of ornamental ferns. Plant Cell, Tissue and Organ Culture, 73, 1–13.CrossRefGoogle Scholar
Gantt, E. and Arnott, H. J. (1965). Spore germination and development of the young gametophyte of the ostrich fern (Matteuccia struthiopteris). American Journal of Botany, 52, 82–94.CrossRefGoogle Scholar
Goel, A. K. (2002). Botanic gardens: potential source for conservation of plant diversity. Journal of Economic and Taxonomic Botany, 26, 67–74.Google Scholar
Hill, R. H. (1971). Comparative habitat requirements for spore germination and prothallial growth of three ferns of southeastern Michigan. American Fern Journal, 61, 171–182.CrossRefGoogle Scholar
Holloway, P. S. and Boyd, D. J. (1993). Spore viability and germination of the endangered Aleutian shield-fern, Polystichum aleuticum. Hortscience, 28, 182A.Google Scholar
Hvoslef-Eide, A. K. (1990). In vitro storage as influenced by growth conditions prior to storage. In Integration of In vitro Techniques in Ornamental Plant Breeding, ed. Fong, F.. Wageningen: Eucarpia Symposium, pp. 124–131.Google Scholar
Jones, L. E. and Hook, P. W. (1970). Growth and development in microculture of gametophytes from stored spores of Equisetum. American Journal of Botany, 57, 430–435.CrossRefGoogle Scholar
Karp, A. (1994). Origins, causes and uses of variation in plant tissue cultures. In Plant Cell and Tissue Culture, ed. Vasil, I. K. and Thorpe, T. A.. Dordrecht: Kluwer, pp. 139–151.Google Scholar
Lebkuecher, J. G. (1997). Desiccation-time limits of photosynthetic recovery in Equisetum hyemale (Equisetaceae) spores. American Journal of Botany, 84, 792–797.CrossRefGoogle ScholarPubMed
Lindsay, S. and Dyer, A. F. (1990). Fern spore banks: implications for gametophyte establishment. In Taxonomía, Biogeografía y Conservación de Pteridófitos, ed. Rita, J. and PalmaMallorca, J.: Societat d'Historia Natural de les Illes Balears IME, pp. 243–253.Google Scholar
Lloyd, R. M. and Klekowski, E. J. Jr. (1970). Spore germination and viability in pteridophyta: evolutionary significance of chlorophyllous spores. Biotropica, 2, 129–137.CrossRefGoogle Scholar
Lusby, P., Lindsay, S., and Dyer, A. F. (2002). Principles, practice and problems of conserving the rare British fern Woodsia ilvensis (L.) R. Br. In Fern Flora Worldwide: Threats and Responses, ed. Dyer, A. F., Sheffield, E., and Wardlaw, A. C.. The Fern Gazette, 16, 350–355.Google Scholar
Miller, J. H. and Miller, P. M. (1961). The effect of different light conditions and sucrose on the growth and development of the gametophyte of the fern Onoclea sensibilis. American Journal of Botany, 48, 154–158.CrossRefGoogle Scholar
Miller, J. H. and Wagner, P. M. (1987). Co-requirement for calcium and potassium in the germination of spores of the fern Onoclea sensibilis. American Journal of Botany, 74, 1585–1589.CrossRefGoogle Scholar
Mottier, D. M. (1914). Resistance of certain fern prothalli to extreme desiccation. Science, 39, 295.Google Scholar
Oliphant, J. L. (1989). In vitro cultivation of Todea babarea –from spore to sporophyte. International Plant Propagators Society Proceedings, 38, 324–325.Google Scholar
Ong, B.-L. and Ng, M.-L. (1998). Regeneration of drought-stressed gametophytes of the epiphytic fern, Pyrrosia pilosellodes (L.) Price. Plant Cell Reports, 18, 225–228.CrossRefGoogle Scholar
Page, C. N. (1979). Experimental aspects of fern ecology. In The Experimental Biology of Ferns, ed. Dyer, A. F.. London: Academic Press, pp. 552–589.Google Scholar
Page, C. N., Dyer, A. F., Lindsay, S., Mann, D. F., Ide, J. M., Jermy, A. C., and Paul, A. M. (1992). Conservation of pteridophytes: the ex situ approach. In Fern Horticulture: Past, Present and Future Perspectives, ed. Ide, J. M., Jermy, A. C., and Paul, A. M.. Andover: Intercept, pp. 269–278.Google Scholar
Pangua, E., García-Álvarez, L., and Pajarón, S. (1999). Studies on Cryptogramma crispa spore germination. American Fern Journal, 89, 159–170.CrossRefGoogle Scholar
Pattison, G. A., Ide, J. M., Jermy, A. C., and Paul, A. M. (1992). National plant collections: ex situ conservation. In Fern Horticulture: Past, Present and Future Perspectives, ed. Ide, J. M., Jermy, A. C., and Paul, A. M.. Andover: Intercept, pp. 305–308.Google Scholar
Pence, V. C. (2000a). Survival of chlorophyllous and nonchlorophyllous fern spores through exposure to liquid nitrogen. American Fern Journal, 90, 119–126.CrossRefGoogle Scholar
Pence, V. C. (2000b). Cryopreservation of in vitro grown fern gametophytes. American Fern Journal, 90, 16–23.CrossRefGoogle Scholar
Pence, V. C. (2001). Cryopreservation of shoot tips of Selaginella uncinata. American Fern Journal, 91, 37–40.CrossRefGoogle Scholar
Pence, V. C. (2002). Cryopreservation and in vitro methods for ex situ conservation of pteridophytes. The Fern Gazette, 16, 362–368.Google Scholar
Pence, V. C. (2003). Ex situ conservation methods for bryophytes and pteridophytes. In Strategies for Survival, ed. Guerrant, E., Havens, K., and Maunder, M.. Covelo, CA: Island Press, pp. 206–227.Google Scholar
Pence, V. C., Sandoval, J., Villalobos, V., and Engelmann, F. (2002). In Vitro Collecting Techniques for Germplasm Conservation. IPGRI Technical Bulletin No. 7. Rome: International Plant Genetic Resources Institute.Google Scholar
Pérez-García, B., Orozco-Segovia, A. and Riba, R. (1994). The effects of white fluorescent light, far-red light, darkness, and moisture on spore germination of Lygodium heterodoxum (Schizaeaceae). American Journal of Botany, 81, 1367–1369.CrossRefGoogle Scholar
Planta Europa (2007). Targets of the EPCS. www.plantaeuropa.org/pe-EPCS-targets.htm.
Proctor, M. C. F. and Pence, V. C. (2002). Vegetative tissues: bryophytes, vascular “resurrection plants” and vegetative propagules. In Desiccation and Plant Survival, ed. Black, M. and Pritchard, H.. Oxford: CAB International, pp. 207–237.CrossRefGoogle Scholar
Quintanilla, L. D., Amigo, J., Pangua, E., and Pajaron, S. (2002). Effect of storage method on spore viability in five globally threatened fern species. Annals of Botany, 90, 461–467.CrossRefGoogle ScholarPubMed
Randi, A. M. and Felippe, G. M. (1988). Efeito do armazenamento de esporos, da aplicação de DCMU e da pré-embebição em PEG na germinação de Cyathea delgadii. Ciência e Cultura 40, 484–489.Google Scholar
Sakai, A., Kobayashi, S., and Oiyama, I. (1990). Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var brasiliensis Tanaka) by vitrification. Plant Cell Reports, 9, 30–33.CrossRefGoogle ScholarPubMed
Simabukuro, E. A., Dyer, A. F., and Felippe, G. M. (1998). The effect of sterilization and storage conditions on the viability of the spores of Cyathea delgadii. American Fern Journal, 88, 72–80.CrossRefGoogle Scholar
Smith, D. L. and Robinson, P. M. (1975). The effects of spore age on germination and gametophyte development in Polypodium vulgare L. New Phytologist, 74, 101–108.CrossRefGoogle Scholar
Stokey, A. G. (1951). Duration of viability of spores of the Osmundaceae. American Fern Journal, 41, 111–115.CrossRefGoogle Scholar
Tannoury, M., Ralambosoa, J., Kaminski, M., and Dereuddre, J. (1991). Cryopreservation by vitrification of coated shoot-tips of carnation (Dianthus caryophyllus L.) cultured in vitro. Comptes Rendus de l'Academie des Sciences, Paris, Serie III, 313, 633–638.Google Scholar
Theuerkauf, W. D. (1993). South Indian pteridophytes – ex situ conservation. Indian Fern Journal, 10, 219–225.Google Scholar
Towill, L. E. and Bajaj, Y. P. S. (2002). Crypreservation of Plant Germplasm. II. Biotechnology in Agriculture and Forestry, 50. Berlin: Springer-Verlag.CrossRefGoogle Scholar
Walter, K. S. and Gillett, H. J. (1998). 1997 IUCN Red List of Threatened Plants. Gland, Switzerland: IUCN The World Conservation Union.Google Scholar
Walters, C. (2004). Principles for preserving germplasm in gene banks. In Ex Situ Plant Conservation: Supporting Species Survival in the Wild, ed. Guerrant, E. O. Jr., Havens, K., and Maunder, M.. Covelo, CA: Island Press, pp. 113–138.Google Scholar
Ward, N. B. (1852). On the Growth of Plants in Closely Glazed Cases, 2nd edn. London: John Van Voorst.CrossRefGoogle Scholar
Wardlaw, A. C. (2002). Horticultural approaches to the conservation of ferns. In Fern Flora Worldwide: Threats and Responses, ed. Dyer, A. F., Sheffield, E., and Wardlaw, A. C.. The Fern Gazette, 16, 356–361.
Whittier, D. P. (1996). Extending the viability of Equisetum hymenale spores. American Fern Journal, 86, 114–118.CrossRefGoogle Scholar
Wilkinson, T. (2002). In vitro techniques for the conservation of Hymenophyllum tunbrigense (L.) Sm. In Fern Flora Worldwide: Threats and Responses, ed. Dyer, A. F., Sheffield, E., and Wardlaw, A. C.. The Fern Gazette, 16, 458.
Windham, M. D., Wolf, P. G., and Ranker, T. A. (1986). Factors affecting prolonged stored spore viability in herbarium collections of three species of Pellaea. American Fern Journal, 76, 141–148.CrossRefGoogle Scholar
Withers, L. A. (1985). Cryopreservation of cultured plant cells and protoplasts. In Cryopreservation of Plant Cells and Organs, ed. Kartha, K. K.. Boca Raton, FL: CRC Press, pp. 243–267.Google Scholar

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