Demographic monitoring is required in threatened species management, yet effective and efficient monitoring is challenging for species that are difficult to capture or susceptible to capture stress. One possible monitoring approach for such species is non-invasive genetic sampling with capture–recapture methods (genetic capture–recapture). We evaluated the performance of genetic capture–recapture in a challenging model system, monitoring the threatened Sonoran pronghorn Antilocapra americana sonoriensis. In an effort to determine the best (i.e. efficient, accurate, precise, cost-effective) method for abundance estimation, we used simulations to examine the optimal genetic capture–recapture faecal sampling design for this population. We simulated encounter histories for 100–300 individuals, with 0.33–3.33 samples/individual/session, in 1–3 sampling sessions. We explored trade-offs between sample size, number of sessions and multi-session (MARK) versus single-session (capwire) closed capture–recapture abundance estimators, and an accurate and precise estimate. We also compared the cost between the genetic capture–recapture approaches and current aerial monitoring methods. Abundance was biased positively in capwire and negatively in MARK. Bias increased and precision decreased with fewer samples/individual/session. Annual genetic capture–recapture monitoring cost was nearly twice the cost of aerial surveys, although genetic capture–recapture methods provided much higher precision. However at the current estimated abundance (c. 200), the same level of precision achieved with aerial methods can be obtained by collecting 0.75 samples/individual in a single session, for an annual cost saving of > USD 4,000. This approach of comparing estimator performance and cost can easily be applied to other systems and is a useful evaluation for managers to implement prior to designing capture–recapture studies.

Protected areas are central to global efforts to prevent species extinctions, with many countries investing heavily in their establishment. Yet the designation of protected areas alone can only abate certain threats to biodiversity. Targeted management within protected areas is often required to achieve fully effective conservation within their boundaries. It remains unclear what combination of protected area designation and management is needed to remove the suite of processes that imperil species. Here, using Australia as a case study, we use a dataset on the pressures facing threatened species to determine the role of protected areas and management in conserving imperilled species. We found that protected areas that are not resourced for threat management could remove one or more threats to 1,185 (76%) species and all threats to very few (n = 51, 3%) species. In contrast, a protected area network that is adequately resourced to manage threatening processes within their boundary could remove one or more threats to almost all species (n = 1,551; c. 100%) and all threats to almost half (n = 740, 48%). However, 815 (52%) species face one or more threats that require coordinated conservation actions that protected areas alone could not remove. This research shows that investing in the continued expansion of Australia's protected area network without providing adequate funding for threat management within and beyond the existing protected area network will benefit few threatened species. These findings highlight that as the international community expands the global protected area network in accordance with the 2020 Strategic Plan for Biodiversity, a greater emphasis on the effectiveness of threat management is needed.

Information on the distribution and abundance of the forest elephant Loxodonta cyclotis is needed to allocate limited resources appropriately and set conservation goals for the species. However, monitoring at large scales in forest habitats is complicated, expensive and time consuming. We investigated the potential of applying interview-based occupancy analysis as a tool for the rapid assessment of the distribution and relative abundance of forest elephants in eastern Cameroon. Using single-season occupancy models, we explored the covariates that affect forest elephant occupancy and detectability, and identified spatial and temporal patterns in population change and occupancy. Quantitative and qualitative socio-demographic data offer additional depth and understanding, placing the occupancy analysis in context and providing valuable information to guide conservation action. Detectability of forest elephants has decreased since 2008, which is consistent with the decline in perceived abundance in occupied sites. Forest elephants occupy areas outside protected areas and outside the known elephant range defined by IUCN. Critical conservation attention is required to assess forest elephant populations and the threats they face in these poorly understood areas. Interview-based occupancy analysis is a reliable and suitable method for a rapid assessment of forest elephant occupancy on a large scale, as a complement to, or the first stage in, a monitoring process.

Understanding the human dimension is critical for effective conservation management of species involved in human–carnivore conflict. There is also a need to recognize who among the local human population is supportive of wildlife conservation. We investigated how local people's attitudes and knowledge of the leopard cat Prionailurus bengalensis are influenced by socio-demographic variables, encounter rates and the nature of encounters with the felid in Taiwan. During June–August 2014 we interviewed 150 residents in Miaoli County in northern Taiwan, where the leopard cat is known to occur. More than half of the participants indicated they were supportive of leopard cat conservation. The majority of those who expressed positive attitudes towards protection of the leopard cat belonged to younger, more educated socio-demographic groups. Negative attitudes towards leopard cat conservation were most prevalent among farmers, who also reported the highest incidence of negative experiences, mainly involving predation of poultry. We provide recommendations to mitigate human–felid conflict, including changes to animal husbandry practices. We also describe how conservation efforts for this species and wildlife in general in Taiwan could be improved, for example through incentive and awareness-raising programmes.

The Roosevelt–Rondon Expedition marmoset Mico marcai was first collected in 1914 and all information on this primate previously came from three skins brought back by this expedition. As a result, M. marcai is categorized as Data Deficient on the IUCN Red List. As the presumed range of M. marcai lies on the path of the advancing arc of deforestation in Brazil, the collection of relevant data to assess the conservation status of this Amazonian species is of some urgency. Here we present the first field data on the distribution and population size of, and threats to, M. marcai, to reassess the species’ conservation status. During 2012–2015 we surveyed the species in the Marmelos–Aripuanã interfluve, and estimated its density using distance sampling. We also used spatial predictive modelling to estimate forest loss within the species range under two deforestation scenarios. We found the marmoset in 13 localities and estimated its extent of occurrence to be 31,073 km2. We estimated the species’ density to be 8.31 individuals/km2 and extrapolated this to estimate a total population of 258,218 individuals (CI 150,705–441,860). Under a business-as-usual deforestation scenario, c. 10,000 km2 of forest, comprising 33% of the species’ range, would be lost in three marmoset generations (c. 18 years), and we, therefore, recommend that M. marcai be categorized as Vulnerable on the IUCN Red List based on criterion A3c. Other Amazonian marmosets require similar reassessment as their ranges also fall in the path of the arc of deforestation.

With an estimated < 50 adult individuals remaining, the Critically Endangered Balkan lynx Lynx lynx balcanicus is one of the rarest, most threatened and least-studied large carnivores. To identify priority conservation areas and actions for the subspecies, during 2006–2014 we conducted 1,374 questionnaire surveys throughout the potential range of the Balkan lynx to (1) evaluate human–lynx interactions and identify potential threats, and (2) determine the probability of site use in 207 grid cells through occupancy modelling. Human–lynx interactions were related mainly to poaching of lynx, and damage to livestock by lynx. Poaching was intense throughout the potential range of the subspecies, apparently having affected 50–100% of the total estimated extant population. Damage to livestock was recorded only in relation to sheep, mainly in the southern part of the lynx's potential range. Occupancy modelling indicated 108 grid cells with high probability of site use, which was affected mainly by increased terrain ruggedness and reduced forest cover. Based on the combined results of our study we identified five priority areas for conservation, as well as in situ habitat protection, community participation in the conservation of the subspecies, and the improvement and implementation of the existing legal framework as the priority conservation actions for the Balkan lynx.

Despite the efforts invested in their conservation, the status of many threatened carnivores in key conservation landscapes remains unknown. The dhole Cuon alpinus is an Endangered social carnivore whose geographical range has contracted by c. 80% since the early 20th century. North-east India is a critical link between South Asian and South-east Asian dhole populations. In this study we compiled presence records of dholes across north-east India, from multiple sources. We also conducted camera-trap surveys in one part of this region, Dampa Tiger Reserve in the state of Mizoram. We examined the influence of ecological and management factors on fine-scale site-use by dholes in Dampa Tiger Reserve, showing a positive association of dhole site-use with sambar Rusa unicolor encounters, distance to the forest boundary and presence of forest department personnel, underscoring the importance of prey and protection. Our findings also highlight the need for targeted, multi-scale assessments of dhole ecology across other sites in north-east India.

The snow leopard Panthera uncia is globally threatened and reliable information on its abundance, distribution and prey species is a prerequisite for its conservation. In October–November 2014 we assessed the distribution of the snow leopard in the recently established Api Nampa Conservation Area in the Nepal Himalayas. Within selected blocks we conducted sign surveys and counted the number of bharal Pseudois nayaur, its principal wild prey, along transects totalling 106 km. We recorded 203 putative snow leopard signs at an encounter rate of 1.91 signs/km. Generalized linear models of the number of signs detected per transect showed that elevation had a positive influence and human activities a negative influence on sign encounter rate; prey abundance had only a weak positive influence on sign encounter rate. Within the effectively surveyed area of c. 200 km2, we counted 527 bharal at an estimated density of 2.28 animals/km2. Recruitment of bharal was low, estimated at 48 kids/100 adult females, most likely a result of poor or overgrazed rangeland. We estimate the total number of bharal in this conservation area to be > 1,000, a prey base that could sustain 6–9 snow leopards. Based on our field observations, we identified human disturbance and habitat degradation associated with extraction of non-timber forest products, livestock grazing, and poaching as the main threats to the snow leopard. Standardized sign surveys, preferably supplemented by sampling with remote cameras or with genetic analysis of scats would provide robust baseline information on the abundance of snow leopards in this conservation area.

Data on the distribution and population size of the Near Threatened Tibetan antelope Pantholops hodgsonii are necessary to protect this species. Ground-based count surveys are usually carried out from a long distance to avoid disturbing the sensitive animals, and on calving grounds or along migration routes where they are seasonally concentrated. This can result in underestimation of population sizes if terrain features obstruct the view and high concentrations of animals make estimating numbers difficult. Here we test the efficacy of unmanned aerial vehicles (UAVs) for gathering population data for the Tibetan antelope. We conducted the study south of a known calving ground, at the foot of Sewu Snow Mountain, in the Chang Tang National Nature Reserve, China. The UAV did not appear to disturb the animals and resulted in more accurate counts than ground-based observations. A total of 23,063 Tibetan antelopes were identified in twelve orthoimages derived from c. 4,000 aerial photographs. In the first flight area 7,671 females and 4,353 calves were identified (proportion of calves: 36.2%). In the second flight area 7,989 females and 3,050 calves were identified (proportion of calves: 27.6%). Two flights over the same area revealed the direction and speed of moving Tibetan antelope groups. Image resolution, which can be controlled with flight planning, was an important factor in determining the animals’ visibility in the photos. We found that UAV-based surveys outperformed ground-based surveys, and that larger UAVs are preferable for this application.

Local attitudes towards carnivores often reflect the degree of damage they are perceived to cause. Consequently, understanding the interactions between people and these species is essential to conservation efforts. This study investigated local perceptions of three Cerrado canid species and current chicken management practices, to identify the potential damage they cause and how this relates to peoples’ attitudes towards these species. Results from structured interviews at 50 ranches in Goiás, Brazil, highlighted that general knowledge about Cerrado canids differed significantly by species, with interviewees unable to correctly answer questions about the hoary fox Lycalopex vetulus and crab-eating fox Cerdocyon thous in comparison to the maned wolf Chrysocyon brachyurus. Chicken coops were identified as the most effective method for preventing predation, yet only 44% of respondents employed this method. Using a perceived predation measure, interviewees reported chicken predation by all three Cerrado canids even though most of these events were stated to occur during the day, outside the species’ active periods. Reported predation events were a strong predictor of attitude. Participants who experienced predation events reported they did not like having a Cerrado canid on their property. However, 86% of the respondents agreed that Cerrado canids should nevertheless be protected. Our findings support the need to incorporate the human dimension in canid and broader carnivore conservation issues.

In tropical regions the extent of agricultural land is increasing rapidly at the expense of natural forest, with associated losses of biodiversity and ecosystem services. Agroforestry has long been proposed as a more sustainable agricultural system, conserving biodiversity while providing significant local livelihoods. In this context, camera traps were deployed to compare communities of large mammals between natural forest (22,272 hours across 24 deployments) and extensively managed coffee forest (19,059 hours, 23 deployments) for the first time in the south-west Ethiopian highlands. Mammal communities in the two forest types were similar in species richness and Shannon diversity but differed in community composition. Significant indicator species of coffee forest were the crested porcupine Hystrix cristata and the Ethiopian hare Lepus fagani, whereas leopards Panthera pardus and civets Civettictis civetta had a preference for natural forest. The number of detections of mammals was higher in coffee forest, where activity patterns were predominantly crepuscular and nocturnal, which may be a direct adaptation to frequent human disturbance. In natural forest, mammal activity peaked during daytime. Despite the high mammal diversity in extensively managed coffee forest, it cannot fully replace natural forest as a habitat for large mammals. We suggest that a balanced landscape mosaic of coffee and natural forest may be a valuable combination for both conservation and coffee cultivation.

To evaluate and improve the involvement of stakeholders in community-based natural resource management, we developed a stakeholder collaboration index. We compared the stakeholders of five Kenyan conservancies by conducting 10 focus group meetings with conservancy management committees and wildlife game scouts. We used the nominal group technique to identify and rank perceptions of the conservancies’ strengths, weaknesses and opportunities, and any threats. The resulting 455 responses were categorized into ecological, institutional or socio-economic themes of ecosystem management. Collaboration index scores ranged from low (0.33) to high (0.95) collaboration, on a scale of 0–1, with a mean of 0.61. Managers and game scouts had varying perceptions of the conservancies but they agreed about major strengths and threats to conservation. The index highlighted shared perspectives between managers and scouts, which could be used as opportunities for increased stakeholder involvement in collaborative management. The stakeholder collaboration index is a potentially useful tool for improving management of environmental conservation programmes.

The Endangered Antillean manatee Trichechus manatus manatus is one of the most threatened aquatic mammal species in Mexico and the wider Caribbean region. The decline of this subspecies is mainly a result of historical exploitation and the impact of current coastal development. The conservation strategies adopted for the Antillean manatee include habitat protection, reduction of the most severe threats, and the rescue of stranded, orphaned or injured individuals and their management in captivity. This latter strategy has produced positive outcomes in some countries but has been the subject of controversy in others, including Mexico. We analyse the benefits and challenges associated with the management of captive manatees in Mexico, and the consequences of a lack of government policy and strategy for the post-rehabilitation release of individuals. We describe the evolution of this controversy from 1997–2017 in Mexico, analyse the consequences and implications for the conservation of the species, and propose an integrated management strategy that could address the issues raised. Although this strategy has been developed in the context of Mexico, it is applicable to management of this species across the Caribbean region.

The Sulawesi civet Macrogalidia musschenbroekii is endemic to the Indonesian island of Sulawesi, where it is the largest mammalian predator. Limited field data means that little is known about the species’ distribution, habitat preferences, conservation status and needs, but it is believed to depend on primary forest. We conducted camera-trap surveys across the forests of North Sulawesi, including in two of its main protected areas: Bogani Nani Wartabone National Park and Tangkoko Nature Reserve. From 148 camera trap stations and 10,371 trap nights, Sulawesi civets were recorded 17 times at 12 stations, and in almost equal numbers in primary forest, secondary forest and farmland, including the first photographic records from both the National Park and Nature Reserve. We also collected data on the Malay civet Viverra tangalunga, an introduced species of Viverridae and potential competitor. Our records (n = 21) revealed that it is established in secondary forest; it only co-occurred twice with the Sulawesi civet. With a lapse of > 20 years since the last field record of the Sulawesi civet, our findings offer new insight into its status and new enthusiasm within the provincial government for its conservation, which has led to an extension of camera-trap research into neighbouring Gorontalo province.

Loss and degradation of natural habitats continue to increase across the tropics as a result of agricultural expansion. Consequently, there is an urgent need to understand their effects, and the distribution and habitat requirements of wildlife within human-modified landscapes, to support the conservation of threatened species, such as felids. We combined camera trapping and land cover data into occupancy models to study the habitat use and space partitioning by four sympatric felid species in an agricultural landscape in Colombia. Land use in the area includes cattle ranching and oil palm cultivation, the latter being an emerging land use type in the Neotropics. Factors determining species occupancy were the presence of wetlands for jaguars (positive effect); water proximity for pumas (positive effect); and presence of pastures for ocelots and jaguarundis (negative effect). Only ocelots were occasionally recorded in oil palm areas. Our results suggest that to align development with the conservation of top predators it is crucial to maintain areas of forest and wetland across agricultural landscapes and to restrict agricultural and oil palm expansion to modified areas such as pastures, which are of limited conservation value. Because there is no spatial segregation between the felid species we studied, conservation strategies that benefit all of them are possible even in modified landscapes.

There is an unprecedented demand for bushmeat in large cities in sub-Saharan Africa, and this is a major threat to many species. We conducted 2,040 interviews in six cities in four West African countries, in forest and savannah settings. We analysed age- and sex-related differences in the frequency of bushmeat consumption. Overall, we found similar patterns in all cities: 62.2% of men and 72.1% of women said they would never eat bushmeat, whereas 12.8% of men and 8.8% of women said they liked bushmeat and ate it regularly. Younger generations of both sexes tended not to eat bushmeat, regardless of their city of origin. This study of the effects of age, gender and geographical location on bushmeat consumption in African cities provides insights regarding which population groups to target in campaigns to change behaviours.