Volatility and drift are problems commonly associated with auxin-like herbicides. Field and greenhouse studies were conducted at Texas A & M University to develop a method of quantifying volatility and subsequent off-target movement of 2,4-D, dicamba, and triclopyr. Rate–response curves were established by applying reduced rates ranging from 4 × 10−1 to 1 × 10−5 times the normal use rates of the herbicides to cotton and soybean and recording injury for 14 d after treatment (DAT) using a rating scale designed to quantify auxin-like herbicide injury. Injury from herbicide volatility was then produced on additional cotton and soybean plants through exposure to vapors of the dimethylamine salt of 2,4-D, diglycolamine salt of dicamba, and butoxyethyl ester of triclopyr using air chambers inside a greenhouse and volatility plots in the field. Injury resulting from this exposure was evaluated for 14 d using the same injury-evaluation scale that was used to produce the rate–response curves. Volatility-injury data were then applied to the rate–response curves so that herbicide rates corresponding with observed injury could be calculated. Using this method, herbicide volatility rates estimated from greenhouse-cotton injury were determined to be 3.0 × 10−3, 1.0 × 10−3, and 4.9 × 10−2 times the use rates of 2,4-D, dicamba, and triclopyr, respectively. Greenhouse-grown soybean developed injury consistent with 1.4 × 10−2, 1.0 × 10−3, and 2.5 × 10−2 times the normal use rate of 2,4-D, dicamba, and triclopyr, respectively. Under field conditions, cotton developed injury symptoms that were consistent with 4.0 × 10−3, 2.0 × 10−3, and 1.25 × 10−1 times the recommended use rates of 2,4-D, dicamba, and triclopyr, respectively. Field soybean displayed injury symptomology concordant with 1.6 × 10−1, 1.0 × 10−2, and 1.1 × 10−1 times the normal use rates of 2,4-D, dicamba, and triclopyr, respectively. This procedure provided herbicide volatility rate estimates that were consistent with rates and injury from the rate–response injury curves. Additional research is needed to ascertain its usefulness in determining long-term effects of drift injury on crop variables such as yield.

Tolerance and yield of ‘H8001 RR’ soybean to single and sequential glyphosate applications made during vegetative and reproductive stages were examined in field trials in 2000 and 2001. In addition, germination and seedling characteristics from harvested seed were assessed. Glyphosate-resistant soybean tolerated single and sequential applications of 1.7 kg ae/ha glyphosate with minimal injury (≤5%), except for an R2 application in 2000 that caused 20% injury. All observed injury was transient and did not adversely affect soybean density, height, seed weight, or seed yield. Germination and seedling growth of harvested seed were similar to those of a nontreated control. These results suggest that glyphosate-resistant soybean can withstand sequential applications of glyphosate during vegetative and reproductive stages without prolonged adverse effects.

Weed scientists are facing research problems, such as invasive weeds, that may require multidisciplinary approaches to solve. One example is jointed goatgrass, a winter annual grass invading winter wheat fields and not easily managed with conventional control tactics. A national research program was started in 1994 to develop jointed goatgrass management strategies. Involving more than 35 scientists with diverse scientific expertise, this national approach fostered cooperative research projects across 11 states. Research involved entomology, economics, plant breeding, plant physiology, genetics, and weed science, leading to successful management systems for jointed goatgrass. To help other scientists organize regional or national programs, we describe development and performance of the jointed goatgrass program as well as suggest ideas for possible improvement. Pivotal to the success of the program was a Steering Committee, whose role was to establish research priorities and coordinate research across the western United States.

The effects of postemergence rimsulfuron, metribuzin, and adjuvant combinations on potato crop safety and weed control were evaluated in field studies conducted at the University of Idaho Aberdeen Research and Extension Center in 1999 and 2000. Rimsulfuron at 26 g ai/ha plus metribuzin at 0, 140, or 280 g ai/ha was combined with nonionic surfactant (NIS), crop oil concentrate (COC), or methylated seed oil (MSO) in a 3 by 3 factorial with two controls. Under cool, cloudy conditions in 1999, initial ‘Russet Burbank’ potato injury was greater when metribuzin was included in the tank mixture than when rimsulfuron was applied alone, regardless of adjuvant. Under warmer conditions in 2000, however, adding MSO or COC to the tank mixture caused more injury than adding NIS. Rimsulfuron did not provide acceptable season-long common lambsquarters control in 1999 (76%) or in 2000 (88%), regardless of adjuvant. Rimsulfuron combined with metribuzin at 140 or 280 g/ha provided ≥95% common lambsquarters control both years, regardless of adjuvant. Among adjuvants, using MSO (1999 and 2000) or COC (2000) in the spray mixture improved common lambsquarters control compared with using NIS. Tuber yield and quality were not reduced as a result of metribuzin rate or adjuvant treatments either year compared with the weed-free control.

Weed science is an important component of pest management. Weeds cause approximately 12% loss in United States crop production, reduce crop quality, poison livestock, and adversely affect human health, recreation, and transportation. Herbicides comprise approximately 65% of pesticide expenditures, whereas insecticides and fungicides each comprise less than 20%. The total effect of weeds, including crop losses and costs of control, in the United States was estimated in 1994 to be $20 billion annually. A survey was prepared and mailed to weed scientists at universities and experiment stations in the northeastern United States to determine the number of faculty positions and course offerings devoted to weed science. There are approximately five times as many entomologists and more than three times as many plant pathologists as weed scientists at universities in the northeast. There are more than six times as many graduate students currently in entomology and more than four times as many in plant pathology compared with weed science. Few undergraduate courses in weed science are taught, and most universities have no graduate classes in weed science. There are almost seven times as many undergraduate entomology courses and more than twice as many plant pathology courses as weed science classes in this region. There are more than 17 times as many graduate entomology courses and more than 15 times as many plant pathology courses compared with weed science graduate classes. There are no departments devoted solely to weed science in the northeast, whereas entomology and plant pathology departments are both common. Most universities have little to no faculty assigned to aquatic, forestry, noncrop weed control, weed ecology, or laboratory trials, and numbers assigned to agronomic and horticultural crop weed management are limited. Additional university resources are needed if weed science research, teaching, and extension efforts are to meet the priority needs in weed management.

Field studies were conducted at the Alabama Agricultural Experiment Station near Shorter, AL, from 1998 to 2000 to evaluate six commercial spray adjuvants and their effects on glyphosate applied to cotton with an air-assisted sprayer. Each adjuvant was evaluated with a conventional sprayer calibrated to deliver 94 L/ha solution and a low-volume, air-assisted sprayer calibrated to deliver 19 L/ha solution. Glyphosate was applied to two-leaf pitted and entireleaf morningglory growing in glyphosate-resistant cotton. Visual control of pitted and entireleaf morningglory was more dependent on the rate of herbicide application than on a particular adjuvant or sprayer, with glyphosate at 0.42 kg ae/ha providing 70% control 21 d after treatment. Pitted and entireleaf morningglory biomass measurements generally reflected visual control data. Ammonium sulfate and formulated glyphosate consistently gave the highest visual control and the greatest biomass reduction. Glyphosate application rate was more important than adjuvant addition or sprayer type, with the higher rates of application providing greater control. No differences in weed control were observed between spray systems; therefore, air-assisted sprayers may be used on a field-scale basis with consistent and adequate results.

Two corn hybrids were evaluated to determine tolerance to foramsulfuron applications with and without the safener, isoxadifen-ethyl at five application timings. The corn hybrid N58D1 was more sensitive to foramsulfuron applications than N59Q9. Averaged across all application timings, the addition of isoxadifen-ethyl decreased corn injury of the more sensitive corn hybrid, N58D1. Foramsulfuron application timing had a significant effect on corn tolerance. The greatest corn injury generally occurred from foramsulfuron applications to V6 and V8 corn (visible collars). Foramsulfuron injury ranged between 9 and 37% from these two application timings, 7 d after treatment (DAT). By 21 DAT, corn generally recovered from foramsulfuron injury, and there were a few cases of reduced corn yield at the end of the season. Yield reductions and ear malformations were greatest at the Urbana location with both hybrids when foramsulfuron was applied to V12 corn. The addition of isoxadifen-ethyl alleviated ear malformations and increased corn yield from this application timing. Applications of foramsulfuron before V6 corn also were important in reducing corn injury and protecting yield. Therefore, when growers are using foramsulfuron for weed control, it will be important to select proper hybrids that are more tolerant as well as making applications before V6 corn.

Field, greenhouse, and laboratory studies were conducted to examine the potential for antagonism of postemergence graminicides when tank-mixed with cloransulam and to determine the role of herbicide absorption and translocation in observed antagonistic responses. Cloransulam antagonized annual grass control with aryloxyphenoxypropionate herbicides fluazifop-P, quizalofop, and the prepackaged formulation of fluazifop-P plus fenoxaprop. Cloransulam did not affect annual grass control with the cyclohexandiones clethodim and sethoxydim. In the greenhouse, increasing the rate of the graminicides was a more effective strategy for overcoming antagonism for quizalofop than for fluazifop-P or fluazifop-P plus fenoxaprop, and success was species dependent. Annual grass control with clethodim, sethoxydim, and glyphosate was not adversely affected by tank mixtures with cloransulam. Control of large rhizome johnsongrass was initially reduced when cloransulam was mixed with sethoxydim, fluazifop-P plus fenoxaprop, or quizalofop. By 6 wk after treatment, control of rhizome johnsongrass was antagonized only when cloransulam was mixed with sethoxydim. Rainfall within 1.5 h of application reduced johnsongrass control with glyphosate and sethoxydim but did not affect activity of the other herbicides. Absorption of 14C-fluazifop-P and 14C-quizalofop into broadleaf signalgrass was not affected by cloransulam 6 or 24 h after treatment. Translocation of 14C-fluazifop-P to broadleaf signalgrass shoot tissue above and below the treated leaf was decreased when fluazifop-P was combined with cloransulam. Translocation of quizalofop was not affected by cloransulam.

When herbicides are applied in mixture, and infestation by weeds is less than expected compared with when herbicides are applied alone, a synergistic effect is said to exist. The inverse response is described as being antagonistic. However, if the expected response is defined as a multiplicative, nonlinear function of the means for the herbicides when applied alone, then standard linear model methodology for tests of hypotheses does not apply directly. Consequently, nonlinear mixed-model methodology was explored using the nonlinear mixed-model procedure (PROC NLMIXED) of SAS System®. Generality of the methodology is illustrated using data from a randomized block design with repeated measures in time. Nonlinear mixed-model estimates and tests of synergistic and antagonistic effects were more sensitive in detecting significance, and PROC NLMIXED was a versatile tool for implementation.

Identification of the appropriate use rate is a critical first step in the herbicide development process because use rates affect product utility, market value, and the various risk assessments within the regulatory review process prior to registration. For a given herbicide to be commercially successful, it must provide consistent and sustained efficacy based on a use rate structure that meets customer requirements over a wide range of conditions. Recently, recommendations have been made that advocate the use of herbicide use rates below those outlined on registered product label text. Such advice tends to be based on field work and predictive models designed to identify specific conditions where reduced herbicide use rates are theoretically optimized as dictated by threshold values with assumed levels of commercially acceptable weed control. Unfortunately, many other studies indicate that the use of reduced herbicide rates is not without variability of herbicide efficacy and economic risk. Consequently, reduced use rate theories and related predictive models are often of limited practical value to growers. Aside from inconsistent performance, weed control strategies based on reduced herbicide use rates are not a solution to prevent or even delay target site resistance. In fact, prolonged use of sublethal use rates may select for metabolic resistance and add future weed management challenges by replenishing the weed seed bank. Much effort in terms of development time and resources are invested before product commercialization to ensure that product labels are easily understood and provide value to growers. In this regard, every effort is made to identify the lowest effective use rate that will consistently control target weeds and lead to economic optimization for both the grower and manufacturer.

The influence of weed seed burial depth on seedling emergence is a common and important topic in undergraduate weed science courses. A laboratory exercise was developed at Iowa State University to actively demonstrate changes in weed seedling emergence with increased seed-burial depth. Twenty-five ivyleaf morningglory and wild mustard seeds were sown in pots in monoculture, and the number of emerging seedlings was counted for 3 wk. The results from two semesters were analyzed to determine whether the experiment was successful in meeting its intended outcomes. Assessment of student perceptions and attitudes indicated that the activity increased students' understanding of weed biology; improved students' critical thinking, reading, and writing skills; and improved students' ability to make scientific observations, interpret graphical information, and analyze and summarize research data.

Genetically engineered varieties of creeping bentgrass, resistant to glyphosate, have been developed. Studies were initiated in 2000 and 2001 to examine the relative competitive lateral spread of several transformed lines of creeping bentgrass, nontransformed controls, and cultivar standards. Five-centimeter-diameter vegetative plugs of creeping bentgrass were transplanted into a 1-yr-old stand of perennial ryegrass in Columbus, OH, and 10-yr-old bermudagrass or 10-yr-old St. Augustinegrass in Loxley, AL. Plots were watered to prevent moisture stress to either the bentgrass plugs or surrounding turf swards. Monthly average diameter of the creeping bentgrass was determined by measuring the longest spread and shortest spread. At the end of the experiment, no differences (P = 0.05) in lateral spread were observed between individual lines of transgenic bentgrass, standard cultivars, and nontransformed control lines. Lateral spread of transgenic lines was similar to or less than their nontransformed parent and the standard cultivars tested. Results indicate that glyphosate-resistant creeping bentgrass lines do not spread laterally more than nontransgenic lines. Therefore, if the glyphosate-resistant creeping bentgrass escaped into surrounding turfgrass swards, the potential for spread would not be greater than other creeping bentgrass cultivars currently in use.

Studies were conducted during a 2-yr period measuring corn silage and grain yield and velvetleaf seed production in response to velvetleaf density. Velvetleaf densities of 0, 2, 5, 10, and 21 plants/m2 were established in conventionally tilled corn. The percent corn yield reduction in response to velvetleaf density was similar for both years despite differences in total corn yield. Corn grain and silage yield responded differently to velvetleaf interference. Although both were adversely affected, silage yield reductions were twice that of grain at the low velvetleaf densities. A hyperbolic yield model predicted a maximum yield loss for corn silage and grain of 36 and 37% with incremental losses of 7 and 3%, respectively, as velvetleaf density increased. Velvetleaf seed production ranged from 2,256 to 4,844 seed/m2 from the lowest to the highest density. This study demonstrates that corn silage yield is more sensitive than corn grain yield to velvetleaf interference, as well as how crop value plays an important role in determining economic thresholds. Finally, this research confirms the prolific nature of velvetleaf and shows that even at low densities, velvetleaf seed production could affect weed control decisions for many seasons to come.

Although the impact of Canada thistle (CT) on annual crop production is relatively well established, few investigations report on this weed's impact within perennial pastures. This field study assessed herbage yield losses within eight central Alberta pastures from 1999 to 2001. Each pasture was sampled in 1999 to quantify thistle and herbage biomass within 25 permanent plots. CT was controlled in 2000 and the response of vegetation measured in 2000 and 2001. Before removal, significant negative relationships (P < 0.05) between thistle abundance and herbage were noted at six sites. After thistle removal, herbage at several sites displayed positive responses. Both thistle density and biomass adequately predicted herbage yield loss. Yield losses due to CT can be substantial, peaking at 2 kg/ha for each kilogram of standing thistle biomass and 4.3 kg/ha with each additional thistle stem per square meter. Demonstrated yield losses were variable among sites however, likely due to factors such as heterogeneity in soils, available moisture, and variation in disturbance history or pasture vegetation composition. CT management in perennial pastures of western Canada may enhance pasture production, but further research is required to reliably predict the ability of pastures to respond.

Field trials were established in orchardgrass and tall fescue grass pastures and hay fields during 2000 and 2001 to evaluate wild carrot, broadleaf plantain, buckhorn plantain, bladder campion, and yellow crownbeard response to a variety of herbicides and prepackaged herbicide combinations. Picloram plus 2,4-D at 0.23 plus 0.84 kg/ha and higher, triclopyr plus clopyralid at 0.96 plus 0.32 kg/ha and higher, and 2,4-D plus triclopyr at 2.20 plus 1.12 kg/ha provided greater than 84% wild carrot, broadleaf plantain, and buckhorn plantain control at 4 mo after treatment (MAT) in both years. Metsulfuron, picloram plus 2,4-D at 0.30 plus 1.12 kg/ha, and 2,4-D plus triclopyr at 2.20 plus 1.12 kg/ha provided from 56 to 61% control of bladder campion 4 MAT, which was the highest bladder campion control observed in these trials. Greater than 80% yellow crownbeard control was achieved during both years with picloram plus 2,4-D at 0.15 plus 0.56 kg/ha and higher and with triclopyr plus clopyralid at 0.96 plus 0.32 kg/ha and higher. Results from these experiments reveal that good broad-spectrum control of many biennial and perennial broadleaf weeds can be achieved with higher rates of the picloram plus 2,4-D and triclopyr plus clopyralid prepackaged combinations.

Field experiments were conducted in 1998, 1999, and 2000 at two locations (Harrow and Ridgetown) in southwestern Ontario to determine the biologically effective rates (I90) of a commercial formulation of flufenacet plus metribuzin for weed control and processing tomato tolerance. At the proposed label use rate, flufenacet plus metribuzin provided excellent (≥90%) early-season (22 to 29 d after planting) control of velvetleaf, good (80 to 89%) control of barnyardgrass and redroot pigweed, and fair (60 to 79%) control of common lambsquarters. Flufenacet plus metribuzin provided fair late-season (59 to 97 d after planting) control of redroot pigweed and common lambsquarters and poor (≤59%) control of barnyardgrass and velvetleaf. At Harrow and Ridgetown, I90 values for early-season weed control ranged from 70 to 1,300 g ai/ha and 50 to 1,900 g ai/ha, respectively. Flufenacet plus metribuzin provided poor weed control at Ridgetown. This result was not attributable to higher weed density or particular weed species but may have been caused by lack of rainfall and too low application rates for the medium-textured soil type. It is estimated that flufenacet plus metribuzin at 1,400 g/ha can control green foxtail season-long, whereas barnyardgrass and common lambsquarters would require 1,900 g/ha. Season-long control of velvetleaf and redroot pigweed would require application rates of 3,200 and 7,100 g/ha, respectively. Only slight early-season crop injury was observed, which was not reflected in yields. Optimum yields of tomatoes were obtained at Harrow at rates lower or slightly higher than the registered rates for corn and soybean. Tomato yields were higher at Harrow than at Ridgetown, which may have been due to differences in soil texture. Tomatoes grown in a medium-textured (Ridgetown) soil appeared to be less competitive against weeds than those grown in a coarse-textured soil (Harrow).

Alfalfa seed producers have a limited number of herbicide options to manage weed problems. MON-37500 (proposed name sulfosulfuron) is a sulfonylurea herbicide that controls dandelion and quackgrass, two common weeds in alfalfa fields. A study was conducted in two alfalfa fields at Valparaiso and Carrot River, Saskatchewan, Canada, from 1999 to 2001 to evaluate perennial weed control and alfalfa production responses with 0.5×, 1×, and 1.5× label-recommended rates of MON-37500 and also 2,4-DB and hexazinone. MON-37500 applied at the 1× and 1.5× rates at both locations reduced mid-May alfalfa vigor from 100% to between 80 and 90% and increased early-season control of dandelion and quackgrass by about 10 to 40 percentage units, when compared with other herbicide treatments. Improved weed control with 1× and 1.5× MON-37500 rates was sustained into mid-June only at Carrot River and was completely eliminated (100% vigor and 0% weed control), or almost so, by mid-July. MON-37500 did not control Canada thistle. Improved early-season weed control with the 1× MON-37500 rate apparently compensated for the loss of alfalfa vigor at Valparaiso, thus resulting in 27% (57 kg/ha) greater seed yield than with the other herbicide treatments. At Carrot River, hexazinone generally provided levels of weed control similar to MON-37500 but did not injure alfalfa. Consequently, alfalfa yields were highest and the proportion of dead (decaying) seed was least with this treatment. The 0.5× MON-37500 rate often resulted in inferior weed control relative to the 1× and 1.5× rates and never was among the herbicide treatments providing the greatest seed yield. Managing the residual activity of MON-37500 and its negative effect on alfalfa growth, especially at locations with soils having coarse texture and low organic matter content, represents the greatest challenge in making MON-37500 a reliable weed management tool for alfalfa seed producers.

Trumpetcreeper, a deciduous, perennial vine found in the midwestern and southeastern United States, causes crop losses through direct competition and by crop entanglement, and control measures include both herbicides and tillage. The regenerative capacity of trumpetcreeper rootstocks of varying length and diameter when planted at different depths was evaluated in greenhouse experiments in Arkansas. Deeper placement of rootstocks delayed trumpetcreeper emergence but had no effect on shoot growth after emergence. Larger rootstock segments produced more shoots per plant and more total biomass production. However, smaller rootstock segments produced more shoots and total biomass per centimeter of rootstock. Overall, decreasing trumpetcreeper rootstock size will delay shoot emergence but may not result in increased long-term control.

Survival of horseweed in several glyphosate-tolerant cotton and soybean fields treated with glyphosate at recommended rates preplant and postemergence was observed in Mississippi and Tennessee in 2001 and 2002. Plants originating from seed collected from fields where horseweed escapes occurred in 2002 were grown in the greenhouse to the 5-leaf, 13- to 15-leaf, and 25- to 30-leaf growth stages and treated with the isopropylamine salt of glyphosate at 0, 0.025, 0.05, 0.1, 0.21, 0.42, 0.84, 1.68, 3.36, 6.72, and 13.44 kg ae/ha to determine if resistance to glyphosate existed in any biotype. All biotypes exhibited an 8- to 12-fold level of resistance to glyphosate when compared with a susceptible biotype. One resistant biotype from Mississippi was two- to fourfold more resistant than other resistant biotypes. Growth stage had little effect on level of glyphosate resistance. The glyphosate rate required to reduce biomass of glyphosate-resistant horseweed by 50% (GR50) increased from 0.14 to 2.2 kg/ha as plant size increased from the 5-leaf to 25- to 30-leaf growth stage. The GR50 rate for the susceptible biotype increased from 0.02 to 0.2 kg/ha glyphosate. These results demonstrate that the difficult-to-control biotypes were resistant to glyphosate, that resistant biotypes could survive glyphosate rates of up to 6.72 kg/ha, and that plant size affected both resistant and susceptible biotypes in a similar manner.

Four field studies were conducted at the Peanut Belt Research Station near Lewiston Woodville, NC, in 2000, 2001, and 2002 to evaluate crop tolerance, weed control, grain yield, and net returns in glyphosate-resistant corn with various herbicide systems. Preemergence (PRE) treatment options included no herbicide, atrazine at 1.12 kg ai/ha, or atrazine plus metolachlor at 1.68 kg ai/ha. Postemergence (POST) treatment options included glyphosate at 1.12 kg ai/ha as either the isopropylamine salt or the diammonium salt, either alone or in mixtures with mesotrione at 105 g ai/ha plus crop oil concentrate at 1% (v/v) or halosulfuron at 53 g ai/ha plus 0.25% (v/v) nonionic surfactant. All response variables were independent of glyphosate formulation. Addition of metolachlor to atrazine PRE improved large crabgrass and goosegrass control but did not always improve Texas panicum control. POST control of these annual grasses was similar with glyphosate alone or in mixture with halosulfuron or mesotrione. Glyphosate POST controlled common lambsquarters and common ragweed 89 and 93%, respectively. Glyphosate plus halosulfuron POST provided more effective yellow nutsedge control than glyphosate POST. Atrazine PRE or atrazine plus metolachlor PRE followed by any glyphosate POST treatment controlled Ipomoea spp. at least 93%. Glyphosate plus mesotrione in total POST systems always provided greater control of Ipomoea spp. than glyphosate alone. The highest yielding treatments always included glyphosate POST, either with or without a PRE herbicide treatment. Similarly, systems that included any glyphosate POST treatment had the highest net returns.