The molecular basis of resistance to tribenuron-methyl, an acetolactate synthase (ALS)–inhibiting herbicide was investigated in four resistant (R) and three susceptible (S) flixweed populations. The resistance level in the R populations was assessed in whole-plant pot experiments in a greenhouse, and resistance indices ranged from 723 to 1422. The ALS genes of the three S populations and four R populations were cloned and sequenced, and the full coding sequence of the ALS gene of flixweed was 2,004 bp. The sequences of the ALS genes of the three S populations collected from Shaanxi, Gansu, and Tianjin were identical. Comparison of the ALS gene sequences of the S and R populations with Arabidopsis revealed that proline at position 197 of the ALS gene was substituted by leucine in R population SSX-2, by alanine in R population SSX-3, and by serine in R populations TJ-2 and GS-2. In another study of two R flixweed populations from Hebei and Shaanxi, resistance was also related to mutation at position 197 of the ALS gene. Both studies confirmed tribenuron-methyl resistance in flixweed in China, with the resistance mechanism being conferred by specific ALS point mutations at amino acid position 197.

Brassicaceae cover crops are gaining attention as potential biofumigants for soil pest suppression because of their ability to release biologically active isothiocyanates (ITCs) and other compounds from hydrolysis of glucosinolates (GSLs). However, biofumigation potential of a Brassicaceae is related to its GSL and ITC profile and GSL to ITC conversion efficiency. Field and laboratory experiments were conducted to evaluate the biofumigation potential of seven Brassicaceae cover crops for weed control in plasticulture tomato and bell pepper. GSL concentration and composition varied among cover crops and between roots and shoots of each cover crop. Similar GSLs were produced in both years by roots or shoots of each cover crop, but GSL concentrations were variable between years. Total GSLs contributed to the soil by incorporation of Brassicaceae cover crop tissues were estimated between 47 to 452 nmol g−1 soil. Highest ITC concentration was detected in soil at 3 h after cover crop incorporation, and concentration decreased at later timings. GSL to ITC conversion efficiency ranged from 1 to 39%, with variation among cover crops and between years. No injury was observed in tomato and bell pepper transplanted 1 wk after cover crop incorporation, indicating the tolerance of tomato and pepper to ITCs released by the cover crops. Early-season yellow nutsedge control from Brassicaceae cover crops was ≤ 53% at 2 wk after transplanting and declined to ≤ 18% later in the season. This research demonstrates that Brassicaceae cover crops have marginal potential for early-season weed control and cannot be used as a weed control practice in commercial tomato and bell pepper production.

Ripgut brome has become a problematic weed in Spain both as a consequence of the continuous cropping of winter wheat through minimal tillage systems and its difficult control with selective herbicides. Ripgut brome populations collected in the regions of Castilla-León and Cataluña, two main cereal-growing areas in Spain, were screened in the greenhouse for response to sulfosulfuron, a selective herbicide for the control of brome grasses in wheat, and to glyphosate, often used as a pre-plant knockdown to control bromes in no-till systems. The fresh weight percentage relative to the untreated controls was calculated for each ripgut brome population and herbicide and was used as a measure of the herbicide response. Results showed variation in fresh weight response to both herbicides among populations. Fresh weight of the populations after sulfosulfuron was applied at the two-leaf stage at a rate of 20 g ai ha−1 varied from 3% in the most susceptible population to 35% in the most resistant; the response was similar (6 to 38%) when the herbicide dose was reduced to half. For glyphosate at 800 g ae ha−1, fresh weight varied from 2 to 25% among populations, but the range of variation in fresh weight response increased as herbicide dose decreased to one half, with rates of from 4% to 90% among populations. The location of the collection site (inside the field or in-margin) showed no differences in response to both herbicides, but there was a statistically significant, geographically correlated differentiation for glyphosate response, with a greater resistance in the populations from Castilla-León. Undamaged plants were found after treatments with both herbicides, showing differences in resistance among plants. The study shows inter- and intrapopulation variability for the response of ripgut brome to sulfosulfuron and glyphosate. The implications for resistance development are discussed within the framework of relationships of the structure of the populations relative to their herbicide response.

Weed control in organic onion production is often difficult and expensive, requiring numerous cultivations and extensive hand weeding. Onion safety and weed control with mustard seed meal (MSM) derived from Sinapis alba was evaluated in greenhouse and field trials. MSM applied at 110, 220, and 440 g m−2 severely injured onions and reduced onion stand by 25% or more when applied from planting to the one-leaf stage of onions in greenhouse trials. MSM derived from mustard cultivars ‘IdaGold’ and ‘AC Pennant’ reduced plant dry weight of redroot pigweed with an effective dose that provided 90% weed control (ED90) of 14.5 and 3.2 g m−2, respectively, in greenhouse trials, whereas the ED90 of MSM from a low-glucosinolate cultivar ‘00RN29D10’ was 128 g m−2, suggesting that glucosinolate content and ionic thiocyanate (SCN−) production contribute to phytotoxicity of MSM. In field trials, weed emergence, onion injury, and onion yield were recorded following single or three sequential applications of MSM from 1.1 to 4.5 MT ha−1 beginning at the two-leaf stage of onions in 2008, 2009, and 2010. By 8 wk after treatment (WAT), onion injury following MSM sequential applications was 10% or less in all 3 yr. Combined over 2008 and 2009, 48 and 68% fewer weeds emerged 3 WAT with MSM at 2.2 and 4.5 MT ha−1, respectively. In 2010, MSM at 2.2 and 4.5 MT ha−1 reduced the number of weeds emerged 4 WAT by 91 and 76%, respectively. MSM treatment did not significantly affect onion yield or size in 2008 and 2009, but in 2010 onion total yield was reduced by 29% by three sequential applications of MSM at 2.2 MT ha−1. MSM has potential to be used as a weed-suppressive amendment in organic production systems, but the risk of crop injury is substantial.

Agricultural production systems that reduce the use of in-crop herbicides could greatly reduce risks of environmental damage and the development of herbicide-resistant weeds. Few studies have investigated the long-term effects of in-crop herbicide omissions on weed seedbank community size and structure. A crop-rotation study was sampled 10 yr after a strictly annual rotation and an annual/perennial rotation were exposed to different in-crop herbicide omission treatments. In-crop herbicides were applied either in all annual crops (control), omitted from oats only, or omitted from both flax and oats. Seedbank densities were greatest when in-crop herbicides were omitted from flax and oats, and this treatment also reduced crop yield. Shannon-Wiener diversity differed among crops in the annual crop rotation and among herbicide omission treatments in the perennial rotation. Herbicide omissions changed the weed-community structure in flax and in wheat and canola crops in the annual rotation enough to warrant alternate control methods in some treatments. The magnitude of the effects on the seedbank parameters depended largely on the competitive ability of the crop in which herbicides were omitted. No yield response to omitting herbicides in oats indicated that standard weed management practices have reduced weed populations below yield-loss thresholds.

The analysis of allelopathic bioassay data commonly encounters two problems: one is the small number of biological replicates and the other is that the parameters used to infer allelopathic effects such as percentage and average time of seed germination are analyzed individually, and consequently, information on the global effects resulting from the cumulative effects of the tested agent (e.g., leaves extract) may be missed. Therefore, we propose an index to analyze several parameters altogether so as to have a better view of the global influence of a donor plant on a receptor, whose interference is more likely to be detected. The global effect index can help to detect allelopathic interferences of one plant on another, allowing for a more accurate interpretation of the data in the actual biological setting.

Integrated weed management tactics are necessary to develop cropping systems that enhance soil quality using conservation tillage and reduced herbicide or organic weed management. In this study, we varied planting and termination date of two cereal rye cultivars (‘Aroostook’ and ‘Wheeler’) and a rye/hairy vetch mixture to evaluate cover-crop biomass production and subsequent weed suppression in no-till planted soybean. Cover crops were killed with a burn-down herbicide and roller-crimper and the weed-suppressive effects of the remaining mulch were studied. Cover-crop biomass increased approximately 2,000 kg ha−1 from latest to earliest fall planting dates (August 25–October 15) and for each 10-d incremental delay in spring termination date (May 1–June 1). Biomass accumulation for cereal rye was best estimated using a thermal-based model that separated the effects of fall and spring heat units. Cultivars differed in their total biomass accumulation; however, once established, their growth rates were similar, suggesting the difference was mainly due to the earlier emergence of Aroostook rye. The earlier emergence of Aroostook rye may have explained its greater weed suppression than Wheeler, whereas the rye/hairy vetch mixture was intermediate between the two rye cultivars. Delaying cover-crop termination reduced weed density, especially for early- and late-emerging summer annual weeds in 2006. Yellow nutsedge was not influenced by cover-crop type or the timing of cover-crop management. We found that the degree of synchrony between weed species emergence and accumulated cover-crop biomass played an important role in defining the extent of weed suppression.

Field and greenhouse experiments were conducted to determine the effect of herbicides on napiergrass growth and control. In greenhouse experiments, hexazinone, glyphosate, and imazapic were applied POST, and carbon dioxide (CO2) assimilation was measured with the use of an open-flow gas-exchange system up to 22 d after treatment (DAT). Carbon dioxide assimilation was reduced to zero, indicating plant death, for hexazinone- and glyphosate-treated napiergrass by 2 and 12 DAT, respectively. Imazapic-treated napiergrass CO2 assimilation declined to a constant rate by 22 DAT, but never reached zero. Field studies at Chula and Ty Ty, Georgia, evaluated herbicides for napiergrass control. Herbicide treatments included autumn-only applications, autumn followed by spring applications, and spring-only applications. All autumn-applied treatments exhibited regrowth in the spring. Plants were not affected by cold winter temperatures. A spade tillage treatment was implemented in January 2010, but was not effective in controlling napiergrass. Spring treatments included split applications of autumn treatments and spring-only treatments of glyphosate, glyphosate plus sethoxydim, and imazapyr. Sequential autumn and spring treatments containing glyphosate at both locations failed to eradicate napiergrass. Imazapyr applied spring achieved 94% plant injury by 34 DAT, and indicated potential napiergrass control. Greenhouse results indicated multiple modes of action could be effective in reducing napiergrass growth, but were inconsistent with field results. Further field studies are needed to derive conclusive methods of napiergrass control.

The introduction of herbicide-resistant crops and postemergence herbicides with a wide action spectrum shifted the research focus from how to when crops should be treated. To maximize net return of herbicide applications, the evolution of weed–crop competition over time must be considered and its effects quantified. A model for predicting the yield trend in relation to weed removal time, considering emergence dynamics and density, was tested on data from glyphosate-resistant soybean grown in cropping systems in Italy and Argentina. Despite an ample variation of weed emergence dynamics and weed load in the four trials, the model satisfactorily predicted yield loss evolution. The estimated optimum time for weed control (OTWC) varied from about 18 d after soybean emergence in Argentina to 20 to 23 d in Italy, with time windows for spraying ranging from 14 to 28 d. Within these limits a single glyphosate application ensures good weed control at low cost and avoids side effects like the more probable unfavorable weed flora evolution with double applications and the presence of residues in grains. Despite the apparent simplicity of weed control based on nonselective herbicides, the study outlines that many variables have to be considered to optimize weed management, particularly for the time evolution of the infestation and, subsequently, a proper timing of herbicide application.

Studies were conducted to determine the growth responses of rice to PRE application of oxadiazon and POST application of bispyribac-sodium. Oxadiazon at 1.0 and 1.5 kg ha−1 and bispyribac-sodium at 0.030 and 0.045 kg ha−1 were applied to four rice varieties (‘IR64’, ‘IR72’, ‘RC09’, and ‘RC18’), which were grown in saturated and aerobic (30% of saturation) soils. Control treatments, where no herbicides were applied, were also included in the study. Shoot and root biomass, and height of rice plants were measured 14 d after application. Phytotoxic effects for both herbicides, including reduced shoot and root biomass, were consistent in all varieties. Rice phytotoxicity symptoms were greater when herbicides were applied to saturated than to aerobic soils. Oxadiazon at 1.0 kg ha−1 reduced rice shoot biomass by 22 to 36% in aerobic condition, and 43 to 56% in saturated condition when compared with the control. Bispyribac-sodium reduced rice shoot biomass by 9 to 17% at 0.030 kg ha−1 in aerobic soil and 23 to 37% in saturated soil. The results of this study suggest that soil water content is an important factor influencing herbicide phytotoxicity in rice, and its influence warrants further research to improve understanding of physiology of phytotoxicity to minimize the effects of these herbicides on crop production.

Goosegrass is a problematic summer annual weed in cotton, soybean, and corn production in the southern United States. Glyphosate is labeled for POST control of goosegrass in glyphosate-resistant (GR) cotton, soybean, and corn production. A population of goosegrass in west Tennessee not controlled by glyphosate was examined in greenhouse and laboratory studies. At 21 days after treatment (DAT), a glyphosate-susceptible (SS) biotype was controlled > 90% with glyphosate at rates greater than 210 g ae ha−1. Comparatively, the GR biotype was only controlled 12% at 210 g ae ha−1. Using goosegrass control data, I50 values for GR and SS biotypes were 868 and 117 g ae ha−1, susceptibility, resulting in a resistance factor (RF) of 7.4. Treatment with glyphosate at 210 g ae ha−1 reduced fresh weight biomass of the SS biotype to 5 g per pot compared to 36 g for the GR biotype. A total of 3,360 g ae ha−1 glyphosate was required to reduce fresh weights of the GR biotype to ∼5 g per pot. Using fresh and dry weight biomass data, I50 values for the GR biotype were 3 to 10 times greater than the SS biotype. On each date from 1 to 6 DAT the SS biotype accumulated higher concentrations of shikimate than the GR biotype. Future research should evaluate strategies for managing GR goosegrass with alternative modes of action. To prevent the spread of resistance, additional research evaluating programs for managing glyphosate-susceptible goosegrass in GR crops is also warranted.

Aminocyclopyrachlor (AMCP) is a synthetic auxin herbicide that controls primarily broadleaf (eudicotyledonous) weeds. Previous research indicates that St. Augustinegrass is unacceptably injured by AMCP. In light of the fact that synthetic auxin herbicides usually are safe when applied to monocotyledons, the mechanism for this injury is not fully understood. Anatomical response of St. Augustinegrass to AMCP was investigated using light microscopy. Apical meristem node tissue responded with callus tissue proliferation, abnormal location and development of the apical meristem, necrosis of the developing vascular tissue, vascular parenchyma proliferation, and xylem gum blockages. Node tissues away from the apical meristem responded with xylem gum blockages and the stimulation of lateral meristems and adventitious root formation. Root tip response to AMCP treatment was characterized by a loss of organization. Root tip apical meristem and vascular tissue maturation was disorganized. Additionally, lateral root generation occurred abnormally close to the root tip. These responses impair affected tissue functionality. Mature tissue was unaffected by AMCP treatment. All of these responses are characteristic of synthetic auxin herbicide treatment to other susceptible species. This research indicates that AMCP treatment results in St. Augustinegrass injury and subsequent death through deleterious growth stimulation and concomitant vascular inhibition.

Field and pot studies were conducted in Central New York to determine the potential weed-management benefits of a buckwheat cover crop grown before winter wheat. Specific objectives were to determine buckwheat residue effects on (1) emergence and growth of winter annual weeds; (2) wheat establishment and yield; and (3) emergence of summer annual weeds in the spring following overwinter seed burial. In a field study, buckwheat was sown at two timings (July or August), mowed, and either incorporated or left on the soil surface. Winter wheat was drilled into buckwheat residue in September and weed and crop growth were monitored. In a complementary pot study, four winter annual weeds were sown in soil removed from buckwheat and bare-soil plots at 0 or 15 d after incorporation and monitored for emergence and early growth. To assess buckwheat residue effects on spring emergence from overwintering seeds, seeds of three weed species were buried in buckwheat residue and bare-soil plots in the fall, exhumed in April, and evaluated for emergence. To investigate the mechanism for possible effects of buckwheat residue on overwintering seeds, two levels each of seed treatment (none or fungicide) and fertilization (none or 170 kg ha−1) were applied before burial. Buckwheat residue had no negative effect on wheat yields but suppressed emergence (22 to 72%) and growth (0 to 95%) of winter annual weeds, although effects were often small and inconsistent. Buckwheat residue had no effect on the emergence of buried weed seeds in spring. However, fungicide treatment enhanced the emergence of Powell amaranth seeds by 12.5 to 25.5% and of barnyardgrass seeds by 0 to 12%. Our results suggest that buckwheat residue can contribute to weed management in wheat cropping systems, but that further studies investigating the mechanistic basis for the inconsistent selective effects of buckwheat residue on weeds are needed before buckwheat use can be optimized.

Decision-making processes must indicate if, how, and when weed control should be practiced. So far, Decision Support Systems (DSSs) for weed control to prevent crop yield losses can guide decisions on “if” and “how.” Experience shows that farmers need a DSS that can also guide when to treat, but this can only be done if the actual weed density observed in the field is known during the crop cycle. Emergence models allow the prediction of daily density, but precision depends on the survey date. This study focuses on the estimation of the date of the survey for the best prediction of the daily density throughout the crop cycle. The predicted daily density of each species can be used by DSSs without any further survey, saving time and money and improving the use of the DSSs. Results showed that the best date is when the actual density of each weed reaches or exceeds 50% emergence, and this is earlier than the critical point date, supporting the validity of the date estimation method. The possibility to provide specific advice for farmers considering a proper mortality rate of weed seedlings is then discussed. The ability to optimize the date of sampling can improve the reliability of decision-making tools for integrated weed management, in agreement with the European Union goal of sustainable use of pesticides and more environmentally sustainable cropping systems through the use of integrated pest management.

Bittercress (Brassicaceae) is one of the most prolific and costly weeds of the container nursery industry. Bittercress accessions from container nurseries throughout the major production zones in the United States were examined and compared with herbarium specimens. The identity of these weedy bittercress species were further explored using sequences of the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) region and the nrDNA region for the COP1-interacting protein 7 (CIP7). Four species of bittercress were detected in the nursery industry of the United States, including New Zealand bittercress, hairy bittercress, flexuous bittercress, and little bittercress. The taxon referred to here as Cardamine flexuosa With. (flexuous bittercress) likely contains two genotypes previously reported as European C. flexuosa and Asian C. flexuosa. Phylogenetic relationships between the four species we examined, particularly in relationship to flexuous bittercress, were not fully resolved by the molecular evidence generated for this study. New Zealand bittercress is nonnative and does not appear in current keys to the species for the United States. Flexuous bittercress is also an alien species, which appears in some U.S. keys but not in all. To aid nurserymen and botanists in identification of these four closely related bittercress species, a key was developed and is accompanied by detailed descriptions and illustrations.

The effect of herbicide rates on weed control and crop yield is the subject of countless and ongoing research projects. Weed seed banks receive very little attention in comparison. The seed bank resulting from 3 yr (2006 to 2008) of single herbicide rates in a cropping system where glyphosate/glufosinate and corn/soybean were rotated or not was evaluated in a field located in St-Augustin-de-Desmaures, Québec, Canada. Field plots under conventional tillage were seeded in corn every year, or corn and soybean (1 yr). These plots received the same herbicide every year or various glyphosate/glufosinate 3-yr sequences. Subplots were sprayed with a single POST application of the recommended rate of glyphosate (900 g ae ha−1) or glufosinate (500 g ai ha−1) or lower rates. Subplots received the same full (1.0×, recommended) or reduced (0.5×, 0.75×) rate every year. After crop harvest in 2008, soil cores were extracted and the weed seed bank was evaluated. Including soybean in the cropping system resulted in lower seed banks compared to those under continuous corn cropping. Including glufosinate in a glyphosate herbicide sequence increased weed seed banks due to the lower efficacy of the glufosinate rates tested at reducing the seed bank of annual grasses. Higher herbicide rates translated into lower seed banks, up to a certain rate. After 3 yr, the lowest seed bank (full glyphosate rates every year) still had 4,339 ± 836 seeds m−2 and was higher than the initial seed bank (2,826 ± 724 seeds m−2).

Field studies were conducted in Knoxville, TN, over a 2-yr period (2007 and 2008) to determine the field dissipation rate and efficacy of pyroxasulfone, acetochlor, dimethenamid, and s-metolachlor to broadleaf signalgrass. Depending on rainfall patterns, pyroxasulfone at 209 g ai ha−1 provided broadleaf signalgrass control of > 75%, which was equal to or superior to acetochlor at 1,740 g ai ha−1, dimethenamid at 1,500 g ai ha−1 and s-metolachlor at 1,420 g ai ha−1. Pyroxasulfone provided residual control into the growing season and provides a tool for resistance management of later-emerging weeds. Herbicide dissipation was rapid in all soils (half-life usually < 20 d), although it was slower in a dry year. The order of herbicide dissipation and half-life in days in the 2 yr was acetochlor (3.5, 5 d) > dimethenamid (5, 9 d) > s-metolachlor (8.8, 27 d) > pyroxasulfone (8.2, > 71 d). There was poor correlation between observed weed control at 45 d after treatment and chemically determined herbicide concentrations at that same time, with ∼ 40% difference in 2007 and ∼ 50% difference in 2008.

Leaf-cover area is a widely required plant development parameter for predictive models of weed growth and competition. Its assessment is performed either manually, which is labor intensive, or via visual inspection, which provides biased results. In contrast, digital image processing enables a high level of automation, thereby offering an attractive means for estimating vegetative leaf-cover area. Nonetheless, image-driven analysis is greatly affected by illumination conditions and camera position at the time of imaging and therefore may introduce bias into the analysis. Addressing both of these factors, this paper proposes an image-based model for leaf-cover area and biomass measurements. The proposed model transforms color images into an illumination-invariant representation, thus facilitating accurate leaf-cover detection under varying light conditions. To eliminate the need for fixed camera position, images are transformed into an object–space reference frame, enabling measurement in absolute metric units. Application of the proposed model shows stability in leaf-cover detection and measurement irrespective of camera position and external illumination conditions. When tested on purple nutsedge, one of the world's most troublesome weeds, a linear relation between measured leaf-cover area and plant biomass was obtained regardless of plant developmental stage. Data on the expansion of purple nutsedge leaf-cover area is essential for modeling its spatial growth. The proposed model offers the possibility of acquiring reliable and accurate biological data from digital images without extensive photogrammetric or image-processing expertise.

Growing crops that exhibit a high level of competition with weeds increases opportunities to practice integrated weed management and reduce herbicide inputs. The recent development and market dominance of hybrid canola cultivars provides an opportunity to reassess the relative competitive ability of canola cultivars with small-grain cereals. Direct-seeded (no-till) experiments were conducted at five western Canada locations from 2006 to 2008 to compare the competitive ability of canola cultivars vs. small-grain cereals. The relative competitive ability of the species and cultivars was determined by assessing monocot and dicot weed biomass at different times throughout the growing season as well as oat (simulated weed) seed production. Under most conditions, but especially under warm and relatively dry environments, barley cultivars had the greatest relative competitive ability. Rye and triticale were also highly competitive species under most environmental conditions. Canada Prairie Spring Red wheat and Canada Western Red Spring wheat cultivars usually were the least competitive cereal crops, but there were exceptions in some environments. Canola hybrids were more competitive than open-pollinated canola cultivars. More importantly, under cool, low growing degree day conditions, canola hybrids were as competitive as barley, especially with dicot weeds. Under most conditions, hybrid canola growers on the Canadian Prairies are well advised to avoid the additional selection pressure inherent with a second in-crop herbicide application. Combining competitive cultivars of any species with optimal agronomic practices that facilitate crop health will enhance cropping system sustainability and allow growers to extend the life of their valuable herbicide tools.

In the Midwestern United States, winter hilling, consisting of two tillage activities per year, is required in vinifera-grape vineyards for winter protection. However, this practice often leads to severe soil erosion and pesticide offsite movement. The effectiveness of wheat straw mulch as a replacement for soil mounding was investigated as a way of providing winter protection and to mitigate pesticide leaching and runoff. A laboratory experiment was conducted where simazine was applied to wheat straw or bare soil and then followed by simulated rainfalls. When compared with bare soil, straw reduced simazine leaching and runoff by 40 and 68%, respectively. Adsorption or interception, or both, of simazine by straw were responsible for this effect. Additionally, straw reduced soil erosion by 95% and would largely reduce simazine runoff associated with sediment displacement. The first simulated rainfall contributed 70 and 34% of total simazine runoff from bare soil and straw, respectively. In conclusion, mulching with straw during winter months to provide winter protection could be an effective practice for controlling simazine offsite movement and soil erosion in vinifera vineyards.