The successful utilisation of recent advances in plant biotechnology for the benefit of mankind, with the avoidance of fundamental mistakes that could lead to environmental disaster, requires wise and balanced legislation. In addition, the conservation of the habitats and germplasm so essential to plant breeding requires carefully planned management of resources. These, however, depend upon an informed public with a sensitivity to, and knowledge and understanding of, the issues at stake. It is the people, after all, who influence decision making by governments, through the ballot box or through the pressure of public opinion. Botanic Gardens have a vital role to play in public education, and have the capacity to become the shop windows for the whole of plant science.

Four examples of public education in plant biology being developed at the Royal Botanic Garden Edinburgh are considered, as follows:

(i) Public information, as exemplified by the provision of information about plants, lectures about plants and the development of ecological plantings and displays relating to agriculture, plant breeding and conservation.

(ii) Contact through art, as exemplified by the Andy Goldsworthy retrospective exhibition of 1990 and the 1991 exhibitions of the work of Redouté and Margaret Stones, in which the media of sculpture and painting inspired by the natural world have been used to build bridges between plant science and the wider public.

(iii) Education in primary schools, as exemplified by the ‘Living in a Rainforest’ project, in which young people and their teachers spend periods of time in a replica of a Bornean longhouse and through this experience develop an understanding of the importance of the balance between people and the complex ecosystem of the rainforest.

(iv) Education in secondary schools, as exemplified by the Science and Plants for Schools project in which rapid cycling Brassica rapa and other materials are used in the development of new and exciting approaches to the teaching of experimental plant science, genetics and breeding.

The ways in which projects such as these can be designed to convey important messages concerning plant breeding, genetic engineering, germplasm and habitat conservation and the role of plants in the world economy are discussed, and ideas for other approaches to public education in the Royal Botanic Garden Edinburgh are outlined.

With the recent advances in gene technology, a substantial premium has been placed on the ability to predict protein structure and mechanism from sequence data alone. This is because the direct experimental approaches are more time and labour intensive, and progress is slower. One area of theoretical investigation which has a clear potential to assist in predictive exercises is the natural evolution of protein structure and sequence.

Some aspects of protein evolution, such as the fact that homology of sequence often denotes common ancestry, are routinely considered in predicting three-dimensional structure and mechanism. However, there are other aspects to this natural process which are either not realised or are not appreciated as potential analytical tools.

This article introduces the different ways protein evolution can be investigated and indicates developing techniques as well as those already well established.

In order to investigate and demonstrate objective modelling of proteins as a basis for drug design, we have sought to model several proteins in particularly persuasive circumstances. This is either (a) by filing the results of the model with an independent institution prior to X-ray determination of their structure, or (b) by using wholly automatic, general and reproducible methods, or (c) most often by both. Results suggest the ability to predict the core of the protein to an accuracy of about 1 Å rms deviation between predicted and experimental all-atom coordinates, and of surface loops in the range 1-4 Å rms deviation. Although the upper end of the latter scale seems disturbing, it turns out that many of the surface loops show such large variations for the same protein as studied by different crystallographic groups, particularly when no common protein is used as a starting point for refinement in both cases. Recognising the dynamic nature of some loops on enzymes, and including in the calculation the ability to handle dynamics over long timescales, allows analysis and refinement of enzyme inhibitors as pharmaceuticals. Here we analyse these aspects, particularly by reference to X-ray crystallography data.

Plants, animals and microorganisms are being manipulated to improve their characteristics for commercial purposes. Some manipulated organisms will be grown under containment and only released to the environment accidentally and, usually, in relatively small numbers. Others, such as crop plants, will be grown on a large scale in the open environment. There is concern that modified organisms may become pests, or will produce pests as a result of mating with other organisms in the environment.

This paper will discuss some of the issues involved in deciding whether genetically modified plants should be released to the environment or not.

The cost of patenting an invention should be incurred only where the patent is likely to give the inventor an economic or a tactical advantage. Where it is practicable, secrecy may be preferable to patenting. If an advantage from patenting can be envisaged, then in Western Europe the inventor can apply either for a European patent under the European Patent Convention or for a national patent. The inventor in plant biotechnology faces a ban on patenting certain inventions, including plant varieties and essentially biological processes for the production of plants. But since this ban is interpreted strictly, there are opportunities for patenting what at first glance might seem not patentable. A patent application must give a written description of the invention that is complete enough for a skilled person to reproduce it. The inventor may be required to supplement the description in a patent specification for a biotechnological invention, by depositing a sample of relevant biological materials. A European patent is treated as a national patent in the country for which it was granted. Since a patent may be invalidated in enforcement proceedings, patenting may turn out to have been a costly mistake.

Iron is a critically important metal for a wide variety of cellular events. The element holds this central position by virtue of its facile redox chemistry and the high affinity of both redox states (iron II and iron III) for oxygen. These same properties also render iron toxic when levels exceed the normal binding capacity of the cell. As a result of this potential toxicity, selective iron chelators are finding an important role in the treatment of iron overload associated with many forms of thalassaemia. In addition, they appear to have potential in treating situations where a local increase in iron concentration causes an unfavourable pathology, for instance, in reperfused tissue (heart disease and stroke) and in Parkinsonian brain. There is also evidence that iron chelators may minimise the toxicity of paraquat and the side effects of bleomycin and doxorubicin.

Non-haem iron enzymes can also be inhibited by iron chelators and consequently such enzymes as ribonucleotide reductase and lipoxygenase can be selectively inhibited. Such inhibitory action is being investigated for the treatment of malaria, neoplastic disease, psoriasis and asthma.

Recent developments in these areas are discussed in the present overview.

A wide variety of normal and malignant mammalian cells, as well as inflammatory cells, release extracellular active oxygen species such as superoxide and hydrogen peroxide. Both these species at low levels can stimulate growth and growth responses in a range of normal and malignant cell types. In order to assess the significance of such findings, superoxide dismutase and catalase were added exogenously to the culture medium of hamster (BHK-21) and rat (208F) fibroblasts (non-transformed or oncogene transformed). This resulted in a reduction of cell proliferation and increased cellular uptake of trypan blue stain, suggesting that superoxide and/or hydrogen peroxide may have important roles as extracellular ‘messengers’ promoting cell proliferation and maintaining cell viability. Superoxide dismutase mimics, like superoxide dismutase itself, are also cytostatic towards these cells and provide a basis for the development of tumour-specific antiproliferative drugs.

Plant biotechnology can serve industrial concerns in two ways; it can lead to new products and to new ways of producing an existing product (i.e. ‘processes’). The technology can be used in any business involved in using or selling plant products. In the agricultural input business molecular genetics is having most effect on the introduction of new variation into crop plants. The first products are likely to involve the introduction of insect and disease resistance and herbicide tolerance; some genes transferred will change product quality. Future applications will involve the control of more complex traits. Biotechnology will also enhance plant breeding via improved technologies (e.g. RFLPs and tissue culture); also the production of hybrids is likely to be enhanced and extended. Early products will also include the production of rDNA microbial products for control of pests and diseases.

Of considerable concern to businesses is whether or not they can make sufficient financial return on plant biotechnology. The reasons behind this concern are the time it takes to introduce a product to the market; the expenditure on meeting regulatory requirements; the overall level of public acceptance of the products and the level of return from the farmer. To ensure a fair return on investment there also needs to be a secure structure of intellectual property protection (patents, plant breeders rights, etc.). Continued success in the application of plant science to agricultural improvement depends on a proper economic environment as well as the advancement of the science.

Advances in biotechnology are creating opportunities to increase food production in developing countries and reduce or eliminate the environmental problems associated with conventional farming practices. International agricultural research centres have used the techniques of modern molecular biology for more than a decade, mostly for tissue culture and the elimination of virus diseases in planting materials. As the centres expand their use of the new tools of biotechnology, the focus of their work is shifting. The centres are playing an even larger role in linking developing countries' scientists with colleagues in public and private sector institutions in the developed world. They are also using the new techniques to speed up the development of pest- and disease-resistant crop varieties. Whenever possible they are developing collaborative links with institutions that have the experience and infrastructure needed to carry out advanced molecular research, thus minimising the need for expensive, highly sophisticated in-house capabilities.

In Chapter 11 of Our Mutual Friend, Dickens noted that “Mr Podsnap had even acquired a peculiar flourish of his right arm in often clearing the world of its most difficult problems, by sweeping them behind him”. With a surname redolent of a kitchen botanist, Mr Podsnap no doubt realised that problems are mere opportunities for the theatrically dextrous. Perhaps the act of sweeping provides forward momentum to capitalise on yet more problems. In plant biotechnology, many of the problems confronting mankind are opportunities to employ exciting science.