The presence of excessive phytic acids in foods exerts undesirable antinutritional effects while their agricultural product is utilized as food for humans and as fodder for animals. In this study, 40 cultivated groundnut genotypes were grown in two years and used to estimate the phytic acid phosphorus (PAP) and inorganic phosphorus (InP) contents. The PAP content differed significantly (P= 0.01) among the genotypes and ranged from 149.3 to 315.0 mg PAP/100 g seed with an average of 227.6 mg PAP/100 g seed. The genotypes TG 17 and TG 67 had the highest (315 mg) and the lowest (149.3 mg) PAP content, respectively. The InP content ranged from 58.7 mg/100 g seed in the SG 99 genotype to 102.6 mg/100 g seed in the TG 40 genotype, with a mean of 82.6 mg/100 g seed. The ratio of InP to PAP varied from 0.24 to 0.56. A significantly higher InP:PAP ratio was found in the genotypes TKG 19A, TAG 24, TG 37A, TBG 39 (TDG 39), TG 51, TG 67 and GG 7, which was due to either an increase in InP content or a decrease in PAP content.

Crop wild relatives are important components of agro-ecosystems as potential gene contributors for crop improvement programmes. Cajanus scarabaeoides (L.) Thou., a pigeonpea wild relative is crossable with cultivated pigeonpea and possesses several beneficial traits. Hundred accessions conserved at the ICRISAT genebank were characterized for 13 quantitative and ten qualitative traits to assess the diversity in the collection. Highly significant genotypic variance for leaflet length, days to 5% maturity, seeds per pod, 100-seed weight, seed protein content and trichome density and length was observed. All C. scarabaeoides accessions used in the present study are the best sources for extra early ( < 80 d to 50% flowering) and early maturity (80–100 d to 50% flowering). Eight accessions (ICP 15692, ICP 15696, ICP 15698, ICP 15699, ICP 15712, ICP 15719, ICP 15732 and ICP 15758) and the control ICP 15695 have produced more than 92% healthy pods per plant and higher number of seed per pod (4–6 seeds). Accessions in cluster 2, 3 and 4 with low mean values for days to 50% flowering were found as the best sources for early flowering and maturity. Accessions in cluster 2 and 3 for seeds per pod and cluster 2 for healthy pods per plant were found as promising sources for use in crop improvement. Mean diversity over all clusters was highest (H= 0.57 ± 0.01) for seeds per pod and lowest for days to 50% flowering (0.48 ± 0.02). Significant negative correlation between pods per raceme and healthy pods per plant ( − 0.213) indicated high pod damage in racemes having more pods. Trichome length had highly significant negative association with healthy pods per plant ( − 0.293). The probability map generated using FloraMap, a GIS tool, revealed the occurrence of C. scarabaeoides quite close to the origin and dispersal of pigeonpea. The probability (>75%) map identified a total of 118 provinces covering 790 districts in Bangladesh, Cambodia, India, Indonesia, Laos, Malaysia, Myanmar, Nepal, Papua New Guinea, Philippines, Thailand and Vietnam as geographical gaps in the collection. Complete passport data including location coordinates should be collected while collecting the germplasm to analyze the spatial aspects of species distribution.

Selecting desired agronomic traits may lead to a loss of genetic diversity in crop species. A molecular investigation was conducted to determine how well a set of olive (Olea europaea L.) accessions sampled in Moroccan traditional orchards represented the entire Moroccan olive diversity range. We therefore collected, in traditional agroecosystems from northern and central Morocco, a total of 88 olive trees chosen for their agronomic traits based on local farmers' knowledge. Using 12 SSR loci, 45 trees (51.1%) had a genotype identical to the ‘Picholine Marocaine’ variety, while the remaining samples were classified into 27 different SSR profiles. Two categories of genotypes were identified: (i) genotypes closely related to the ‘Picholine Marocaine’ variety and probably resulting from intensive vegetative propagation from a limited number of clones, and (ii) genotypes displaying a high number of dissimilar alleles which may have originated from selected spontaneous seedlings. A significant difference in allelic richness was revealed between the 28 on-farm selected genotypes and the overall olive diversity, represented by 57 local genotypes, indicating that the on-farm selected trees represented a subsample of Moroccan genetic diversity. This could be explained by the prevalence of ‘Picholine Marocaine’ in traditional orchards, while some original genotypes with favourable agronomic traits resulting from local farmers' selection were also identified. Applying an ethnobotany approach combined with criteria to fulfil farmers' household needs could be particularly relevant and better explain the obtained results.

Flavour is one of the most highly demanded consumer traits of tomato at present; poor flavour is one of the most commonly heard complaints associated with modern varieties of tomato. In order to combine flavour with other desirable fruit traits in improved cultivars, it is important to determine how much variability exists in the crucial compounds that contribute most to flavour. The objective of the present study was to determine the variability of flavour-contributing components including total soluble solids (TSS) and total titratable acids (TTA) among other subjective traits related to flavour in a core collection of tomato accessions. The core collection was comprised of 173 tomato accessions with a wide genetic background from the United States Department of Agriculture (USDA), Agricultural Research Services (ARS) Plant Genetic Resources Unit repository. The TTA varied from 0.20 to 0.64%, whereas the TSS ranged from 3.4 to 9.0%, indicating the availability of broad variation for these traits. Rinon (PI 118783), Turrialba, Purple Calabash and LA2102 were among the high TTA (>0.45%) containing accessions, whereas those with high TSS (>7.0%) were AVRDC#6, Sponzillo and LA2102. A positive correlation of overall flavour with TTA (r= 0.33; P< 0.05) and TSS (r= 0.37; P< 0.05) indicated that these two components play an important role in determining the overall flavour in tomato. Subjectively measured other traits including fruity odour and fruity flavour had positive correlations with overall flavour. Overall flavour is discussed in the context of other traits including fruit firmness. Information obtained from this study may be useful for tomato breeders aiming to improve tomato flavour.

Natural hybridization between wild/weedy and crop species often results in rare hybrids, which can be more weedy and difficult to control. Moreover, the advent of transgenic crop plants raises questions of biosafety risk assessment on the consequences of rare hybrids with possible fitness enhancing genes on the environment. This study aimed at measuring the fitness components of wild–crop sorghum hybrids for various juvenile survival and adult morphological and fertility characters as part of the risk assessment of transgenic sorghum in Africa where the crop was believed to have first domesticated and serves as the major staple. Out of a pool of hybrids made in 2010 from 23 wild sorghum accessions and two released cultivated sorghum varieties using hand emasculation techniques, seven were selected for the field study of their fitness components in 2011. The study confirmed that crop–wild hybrids of sorghum are fertile. Two approaches were followed (relative fitness and mid-parent heterosis) which showed that most of the hybrids were as fit as their wild parents, and in some cases they showed mid-parent heterosis for the measured traits. The results of this study highlighted a potential risk that hybrids carrying crop genes (including herbicide resistance transgenes) could pose because they could be more weedy than their wild/weedy parents if transgenic sorghum is deployed in regions where the wild and cultivated sorghum populations coexist, such as in Ethiopia and in other parts of Africa.

Genebanks serve as a rich source of diversity that can be exploited for crop improvement. However, large numbers of accessions usually have to be evaluated to find material with the characters of interest, and therefore, enhanced trait information can facilitate the more efficient selection of accessions by users. In this study, we report on the distribution of resistances to 28 races of downy mildew among 1223 genebank accessions of cultivated lettuce (Lactuca sativa L.) and 14 related wild species. Due to modern plant breeding, the overall level of resistance of cultivars released after 1950 appears to have increased two- to three-fold compared with varieties from earlier periods. Although fully resistant reactions could be observed among the accessions of cultivated lettuce for each of the 28 investigated races, the resistance probability was more than two-fold higher on average for accessions from the wild gene pool. In general, species of the primary gene pool appeared less resistant than those of the secondary or tertiary gene pool. Probabilities for examined Lactuca species ranged from 0.29 for L. serriola to 1.00 for L. perennis compared with 0.19 for cultivated lettuce, with lower overall resistance probabilities observed only for L. altaica, L. dregeana and L. tenerrima. For L. serriola, the closest relative of cultivated lettuce and the wild species with the highest number of examined accessions, resistance probabilities to each of the investigated downy mildew races were relatively high for populations originating from Eastern Europe and Northern Asia.

Improvement in multi-cut forage sorghum varieties that can perform better than SSG 59-3, a sweet Sudan grass released in 1974, has been a challenge. Efforts were made to create variability in SSG 59-3 through mutagenesis using both physical and chemical mutagens. Fifteen such mutants were evaluated for 2 years in different locations in India. Considerable variability was observed for important fodder yield and quality traits. SSG 237 flowered 10 d early than SSG 59-3. SSG 231, SSG 260, SSG 232 and SSG 237 had high protein percentage and in vitro dry matter digestibility (IVDMD) values compared with SSG 59-3. Hydrocyanic acid (HCN), a major anti-nutritional factor, was low in six mutants, the lowest being in SSG 226. This variability can be used in different breeding programmes aimed at improving multi-cut forage sorghum varieties. The lines with improved fodder quality (low HCN, high protein and IVDMD) can be used in the breeding programme for the improvement in multi-cut forage sorghum varieties.

Low temperature is a major constraint for crop productivity. To cope with this challenge, plants have developed several mechanisms to adapt to low temperature. Developing breeding strategies to enhance cold stress tolerance in crops requires an understanding of the mechanisms by which plants perceive and transmit cold stress-related signals to their cellular machinery, thereby activating adaptive responses. Only one quantitative trait locus for tolerance to low-temperature germination, qLTG3-1, has been narrowed down to the gene level in rice. A 71 bp indel that can be used to distinguish between tolerant and susceptible parents has been identified. We tested the 71 bp indel on 65 diverse rice genotypes including those adapted to colder climates of North and Northeastern India to find evidence of the tolerant allele (insertion) and to see whether it is associated with low-temperature germinability in these genotypes. Our results show that 48% of the rice genotypes tested carried the tolerant allele. The insertion was found to be significantly associated with cold tolerance during germination. Moreover, several landraces/improved varieties known for their superior performance in other abiotic stress conditions such as drought and high salinity conditions, and which were previously never exposed to low temperature, carry the beneficial allele for qLTG3-1, suggesting an additional role of this allele in adverse climatic conditions. This study enhances current understanding of the distribution of the tolerant allele qLTG3-1 in rice germplasm, which could help in the identification of suitable donors for potential marker-assisted breeding programmes.

Lens culinaris Medik. ssp. culinaris is the only cultivated species in the genus Lens. Intensive selection pressure to develop new cultivars, a narrow genetic base, co-evolution of pathogens to partially resistant cultivars and other factors have accelerated susceptibility to different fungal diseases in this species. Few sources for resistance to stemphylium blight (SB) caused by Stemphylium botryosum Wallr. exist among commercial lentil cultivars. A total of 70 accessions were selected from seven species of the genus Lens to screen for SB resistance. The L. culinaris accessions were screened in four different environments, and the accessions of Lens ervoides, L. culinaris ssp. orientalis, Lens tomentosus,Lens nigricans, Lens odemensis and Lens lamottei in growth chamber or greenhouse experiments to identify resistance sources for potential use in lentil breeding. A highly aggressive isolate of SB was used as an inoculum to screen them under controlled conditions. Lentil cultivars ‘Eston’ (resistant) and ‘CDC Glamis’ (susceptible) were used as checks with consistent results in all experiments. Most of the L. culinaris accessions were susceptible to SB, whereas more than 70% of the wild lentil accessions had disease severity scores equal to or significantly lower than that of the SB-resistant check ‘Eston’. Some wild species accessions previously identified with resistance to anthracnose (Colletotrichum truncatum) and ascochyta blight (Ascochyta lentis) were also highly resistant to SB. The highest frequency of resistance to SB was found in L. lamottei followed by L. ervoides of the secondary gene pool. These sources can potentially be used to develop new commercial cultivars with multiple or single disease resistance.

The study of the genetic structure of cultivated plant populations maintained by farmers is of great importance for evolutionary and conservation biology. Such studies help understand the bases of crop evolution and conservation in relation to farmers' practices. In this study, we assessed the genetic structure underlying landrace diversity of dry-season sorghum. This crop constitutes a historical model of innovation developed by farmers to extend sorghum cultivation to the dry season. Two types of dry-season sorghum are cultivated. We aimed to assess the link between farmers' taxonomy and molecular genetic structure. We collected both types of dry-season sorghum in two villages of northern Cameroon which represented 20 landraces. These landraces were genotyped using eight polymorphic microsatellite markers. This study compared two clustering methods: a Bayesian method (STRUCTURE) which is based on explicit genetic assumptions and the discriminant analysis of principal component method. The latter, more recently proposed, is based on the combination of principal component analysis and discriminant analysis. We noticed a general congruence between these two methods. We also used both methods to infer the genetic structure of our sample. Our results showed strong genetic structuring of the landraces, with K= 14 genetic clusters. We then analysed the fit between farmers' taxonomy and genetic structure. The data suggested that each type and each landrace corresponds to a given genetic entity. This pattern was robust across both villages, despite differences in cultural practices.

Botrytis grey mould (BGM), caused by the fungus Botrytis cinerea Pers. ex Fr., is an important disease of chickpea causing economic losses across the world in chickpea-growing regions. There are no available resistance sources in cultivated chickpea against this disease. Cicer echinospermum and C. reticulatum, the only two compatible annual wild species, have been reported to have resistance to BGM. Hence, interspecific populations were developed with susceptible cultivars as female parents and C. echinospermum accession IG 73 074 and C. reticulatum accession IG 72 937 as the pollen donors to transfer and assess the nature of genetic control for BGM. Screening the progeny indicated that resistance to BGM was controlled by a single additive gene/allele (bgmr1cr and bgmr1ce), which can be introgressed through a backcross breeding programme.

India is the second most populous nation in the world after China, and its plant genetic resources are an important basis for crop improvement to meet human population needs. An important legume in the diet of the Indian population is common bean (Phaseolus vulgaris L.). Common beans are one of the many important legumes grown in India, but unlike others, its centre of origin is not in Asia but in the Americas. The objective of this study was to evaluate two collections of Indian common beans: one for an internationally available collection of Food and Agriculture Organization (FAO)-protected accessions and one from the north-western Himalayan region. In total, 149 Indian landraces were evaluated with a total of 24 microsatellites across the two collections, and these represented all common bean-growing states of India. A population structure analysis was used to find groups in each collection, and this was compared across the collections. The genetic analysis of the two sets of Indian accessions with neighbour-joining trees and principal component analysis categorized the landraces into Andean and Mesoamerican gene pool groups. The Andean genotypes dominated the north-western Himalayan collection while the FAO-protected accessions were predominantly Mesoamerican. The Indian subcontinent can be considered as a region of high bean diversity; however, very little introgression was observed between the gene pools in both the germplasm sets. Gene pool identity was further substantiated by the comparison of seed traits, particularly seed size. The role of the landraces in plant breeding programmes is discussed.

Bananas and plantains (Musa spp.) constitute staple food for over 20 million people in Democratic Republic of Congo (DRC). Since 1960, DRC is considered as a secondary centre of plantain diversification with few unknown accessions kept in the INERA-Mulungu genebank. Through similarity coefficients, cluster (unweighted pair group method with arithmetic mean, single, complete, sequential, agglomerative, hierarchical and nested design/clustering procedure) and/or multivariate analyses, numerical morpho-taxonomy has established that this diversity is composed of 37 different accessions. Each accession expressed 98 characters among the 401 possible character states, thus providing 39,298 feature patterns (data points). The 98 characters included 32 vegetative and 66 male and female inflorescences. The accessions were clustered into three genomic groups (AAA, AAB and AABB). Subjective classification ascertained nine subgroups: AAB-Silk, AAB-Pome, AAB-Plantain, AABB-Pisang Awak, AAA-Cavendish, AAA-Ibota, AAA-Gros Michel, AAA-Green-Red and AAA-Lujugira-Mutika. Three subgroups were further divided into nine clone sets which consisted of: Dwarf and Giant Cavendish, French and Horn Plantains, and Musakala, Nfuuka, Nakitembe, Nakabululu and Beer/Mbidde within Lujugira-Mutika. Numerical morpho-taxonomy effectively indicated a relationship between the DRC and Tanzania's Musa diversity. For example, the accessions ‘Kamaramasengi’ and ‘Isangi’ were found to be similar to ‘Kisukari’ (AAB-Silk) and ‘Ngego I’ (AAB-French) common in the Tanzanian Southern Highland. Likewise, the accessions Kimalindi-fupi, Kimalindi-ndefu and Jamaica of Tanzania were duplicates of Bakurura (Kigurube), Cavendish of Butuza and Gros Michel in DRC, respectively. Moreover, numerical morpho-taxonomy confirmed the pedigree of AAB-Prata (Cibwalo) in FHIA 17 and FHIA 23 and the closeness of the ancestors of Yangambi Km5 and Gros Michel. Furthermore, numerical morpho-taxonomy established AA-Mshale malembo as one of the AAA-Lujugira-Mutika parents. Molecular investigations are finally required to confirm the genomes.

The evolutionary diversity of wild potato species makes them excellent materials for improving the narrow genetic basis of the cultivated potato Solanum tuberosum. Understanding their genetic diversity is important not only to choose the best parents for breeding, but also to design proper crossing schemes and selection strategies. The objectives of this study were to determine the resistance response to Ralstonia solanacearum, Potato virus Y and low temperatures of 21 clones of 12 potato species, and to determine their genetic diversity through simple sequence repeat (SSR) markers. Sources of resistance have been found for all the investigated traits, with high resistance variability not only between but also within species. Combined resistances were also identified, with positive implications for efficient breeding. SSR analysis allowed the detection of 12 loci and 46 alleles across all genotypes, with an average value of 3.8 alleles per locus. Both unique and rare alleles useful for marker-assisted selection were found. SSR-based cluster analysis revealed that resistant genotypes were distributed among all clusters, suggesting that genetically different resistant genotypes were identified. The information obtained in this study is discussed from a breeding perspective.

The National Institute of Agrobiological Sciences' (NIAS) Genebank is the coordinating institute in Japan for the conservation of plant, microorganism and animal genetic resources related to food and agriculture. The NIAS Genebank manages this activity in collaboration with sub-banks located across Japan and acts as the central bank. It is important for genebanks to maintain the viability of genetic resources during conservation. Generally, seeds are stored at low temperature and low humidity. Germination percentage is used to monitor seed viability. We have developed data-management software to manage germination testing data in the NIAS Genebank. The software can monitor the pattern of the loss of seed viability of each accession and also support the selection of accessions that need regeneration of seeds. Web-based plant accessions search software displays the latest data on the availability and germination percentages of accessions.

The National Bureau of Plant genetic Resources (NBPGR), New Delhi carries out the registration of unique crop germplasm in order to protect the intrinsic intellectual property as well as to facilitate greater utilization of germplasm in crop improvement programmes. It is therefore imperative to enhance access to information on registered crop germplasm. Here, we present a concept of a search engine that can suffice dual functions of a Web-based and portable search application. The concept entails converting raw data through a series of transformations from a Microsoft-Excel to eXtensible Markup Language (XML) data format. XML data initialized on compatible Web browsers are then queried for the search term based on a looping regular expression matching. The results are then loaded onto the browser in a tabulated output. The concept is implemented in the form of ‘Inventory of Registered Crop Germplasm’ on the Web as well as on a portable memory (compact disk or flash drive). The portable search engine works with minimal hardware and software requirements to enable its widespread utility to ensure greater access to information on registered crop germplasm. The portable search engine can be obtained from the NBPGR, New Delhi and the Web-based search engine can be accessed at http://www.nbpgr.ernet.in/IRCG/index.htm.

The NIASGBsnp system manages data on single nucleotide polymorphisms (SNPs) of rice (Oryzasativa L.) genetic resources in the National Institute of Agrobiological Science (NIAS) Genebank. NIASGBsnp currently holds data on 768 SNP markers for 301 rice accessions and plans to add the SNP data of active rice accessions in the NIAS Genebank. It can show differences between accessions by graphical genotyping. Passport, characteristics and evaluation data of accessions can be retrieved to allow phenotype to be associated with genotype. NIASGBsnp will support various research purposes such as genomic selection and plant pathology research.