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Genetic diversity among INERA-Mulungu (DR Congo) Musa spp. germplasm and their relatedness to those in Tanzania using numerical taxonomy

Published online by Cambridge University Press:  29 November 2012

D. B. Nzawele ,
C. L. Rweyemamu  and
A. P. Maerere
D. B. Nzawele*
Affiliation:
Department of Crop Science and Production, Faculty of Agriculture, Sokoine University of Agriculture, PO Box 3005, Chuo Kikuu, Morogoro, Tanzania
C. L. Rweyemamu
Affiliation:
Department of Crop Science and Production, Faculty of Agriculture, Sokoine University of Agriculture, PO Box 3005, Chuo Kikuu, Morogoro, Tanzania
A. P. Maerere
Affiliation:
Department of Crop Science and Production, Faculty of Agriculture, Sokoine University of Agriculture, PO Box 3005, Chuo Kikuu, Morogoro, Tanzania
*
*Corresponding author. E-mail: dowiya@yahoo.com
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Abstract

Bananas and plantains (Musa spp.) constitute staple food for over 20 million people in Democratic Republic of Congo (DRC). Since 1960, DRC is considered as a secondary centre of plantain diversification with few unknown accessions kept in the INERA-Mulungu genebank. Through similarity coefficients, cluster (unweighted pair group method with arithmetic mean, single, complete, sequential, agglomerative, hierarchical and nested design/clustering procedure) and/or multivariate analyses, numerical morpho-taxonomy has established that this diversity is composed of 37 different accessions. Each accession expressed 98 characters among the 401 possible character states, thus providing 39,298 feature patterns (data points). The 98 characters included 32 vegetative and 66 male and female inflorescences. The accessions were clustered into three genomic groups (AAA, AAB and AABB). Subjective classification ascertained nine subgroups: AAB-Silk, AAB-Pome, AAB-Plantain, AABB-Pisang Awak, AAA-Cavendish, AAA-Ibota, AAA-Gros Michel, AAA-Green-Red and AAA-Lujugira-Mutika. Three subgroups were further divided into nine clone sets which consisted of: Dwarf and Giant Cavendish, French and Horn Plantains, and Musakala, Nfuuka, Nakitembe, Nakabululu and Beer/Mbidde within Lujugira-Mutika. Numerical morpho-taxonomy effectively indicated a relationship between the DRC and Tanzania's Musa diversity. For example, the accessions ‘Kamaramasengi’ and ‘Isangi’ were found to be similar to ‘Kisukari’ (AAB-Silk) and ‘Ngego I’ (AAB-French) common in the Tanzanian Southern Highland. Likewise, the accessions Kimalindi-fupi, Kimalindi-ndefu and Jamaica of Tanzania were duplicates of Bakurura (Kigurube), Cavendish of Butuza and Gros Michel in DRC, respectively. Moreover, numerical morpho-taxonomy confirmed the pedigree of AAB-Prata (Cibwalo) in FHIA 17 and FHIA 23 and the closeness of the ancestors of Yangambi Km5 and Gros Michel. Furthermore, numerical morpho-taxonomy established AA-Mshale malembo as one of the AAA-Lujugira-Mutika parents. Molecular investigations are finally required to confirm the genomes.

Keywords

bananagenebanks (DRCTanzania)genomic groupnumerical morpho-taxonomyplantain
Type
Research Article
Information
Plant Genetic Resources , Volume 11 , Issue 1 , April 2013 , pp. 50 - 61
Copyright
Copyright © NIAB 2012

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References

Amorim, EP, Vilarinhos, AD, Cohen, KO, Amorim, VBO, Santos-Serejo, JA, Silva, SO, Pestana, KN, Santos, VJ, Paes, NS, Monte, DC and Reis, RV (2009) Genetic diversity of carotenoid-rich bananas evaluated by diversity arrays technology (DArT). Genetics and Molecular Biology 32: 96–103.CrossRefGoogle ScholarPubMed
Blench, R (2009) Bananas and plantains in Africa: re-interpreting the linguistic evidence. Ethnobotany Research & Applications 7: 363–380.Google Scholar
Boonruangrod R (2008) Application of cytoplasmic and nuclear genome based marker systems for studying the diversity of present day edible bananas. PhD Thesis, Universität für Bodenkultur.Google Scholar
Christelová, P, Valárik, M, Hribová, E, De Langhe, E and Doležel, J (2011) A multi gene sequence-based phylogeny of the Musaceae (banana) family. BMC Evolutionary Biology 11: 103–115.Google Scholar
De Langhe, E (1961) La taxonomie du bananier plantain en Afrique équatoriale. Journal d'Agriculture Tropicale et de Botanique Appliquée 8: 417–449.Google Scholar
De Langhe, E and Valmayor, RV (1980) French plantains in South East Asia. IBPGR/SEAN 4: 3–4.Google Scholar
De Langhe E, Karamura D and Mbwana A (2001) Tanzania Musa Expedition 2001. Rome: INIBAP/IPGRI, Future harvest, 107 pp.Google Scholar
De Langhe, E, Pillay, M, Tenkouano, A and Swennen, R (2005) Integrating morphological and molecular taxonomy in Musa: the African plantains (Musa spp. AAB group). Plant Systematics and Evolution 255: 225–236.Google Scholar
De Langhe, E, Hribová, E, Carpentier, S, Doležel, J and Swennen, R (2010) Did backcrossing contribute to the origin of hybrid edible bananas? Annals of Botany 106: 849–857.Google Scholar
Evers, G (1992) Banana cultivar diversity in the area of Morogoro, Tanzania. Fruits 47: 377–391.Google Scholar
IPGRI (1996) Descriptors for Banana (Musa spp.). Rome: IPGRI.Google Scholar
Karamura D (1999) Numerical taxonomic studies of the East African highland bananas (Musa AAA-East African) in Uganda. PhD Thesis, University of Reading.Google Scholar
Maerere AP, Rweyemamu CL, Sibuga KP, Mgembe ER, Rwambali E, Nchimbi-Msolla S (2010a) Analysis of the agriculture science, technology and innovation systems: a case study of banana in Tanzania. Acta Horticulturae 879: 851–858.Google Scholar
Maerere AP, Msogoya TJ, Mgembe ER, Mwaitulo S, Mtui HD, Mbilinyi L (2010b) Comparison of yield performance of improved and local banana (Musa) cultivars in Eastern zone of Tanzania. In: Batamuzi EK, Gimbi DM, Max RA, Nombo CI, Msoffe GE (eds) Proceedings of the Fourth Annual PANTIL Scientific Conference, 19–21, October 2009, Morogoro, Tanzania, pp. 172–177.Google Scholar
Miller, RNG, Passos, MAN, Menezes, NNP, Souza, MT Jr., Costa, MMC, Azevedo, VCR, Amorim, EP, Pappas, GJ Jr. and Ciampi, AY (2010) Characterization of novel microsatellite markers in Musa acuminata subsp. burmannicoides, var. Calcutta 4. BMC Research Notes 3: 148–153.CrossRefGoogle ScholarPubMed
Mobambo, KN and Naku, M (1993) Situation de la cercosporiose noire des bananiers et plantains Musa spp. Sous différents systèmes de culture à Yangambi, Haut-Zaïre. Tropicultura 11: 7–10.Google Scholar
Mobambo, P, Staver, C, Hauser, S, Dheda, B and Vangu, G (2010) An innovation capacity analysis to identify strategies for improving plantain and banana (Musa spp.) productivity and value addition in the Democratic Republic of Congo. Acta Horticulturae 879: 821–828.CrossRefGoogle Scholar
Msogoya, TJ, Maerere, AP, Kusolwa, PM and Nsemwa, LT (2006) Field performance of improved banana cv FHIA 17 and FHIA 23 in the Eastern zone of Tanzania. Journal of Agronomy 5: 533–535.Google Scholar
Msogoya, TJ, Grout, B and Maerere, AP (2010) Altered yield and fruit quality of micropropagation-induced off-type of East African Highland banana (Musa AAA East Africa). In: Batamuzi, EK, Gimbi, DM, Max, RA, Nombo, CI and Msoffe, GE (eds) Proceedings of the Fourth Annual PANTIL Scientific Conference, 19–21, October 2009. Morogoro, Tanzania, pp. 231–235.Google Scholar
Mtengeti EE (2008) Residual effects of minjingu phosphate rock and triple superphosphate in some highly weathered and volcanic soils of Tanzania. PhD Thesis, Sokoine University of Agriculture.Google Scholar
Nsabimana A (2006) Establishing genetic diversity of Rwanda highland banana using random amplified polymorphism DNA markers. PhD Thesis, University of Kwazulu-Natal.Google Scholar
Nzawele, DB, Rweyemamu, CL and Maerere, AP (2009) Banana (Musa spp. Colla) cropping systems, production constraints and cultivar preferences in Eastern Democratic Republic of Congo. Journal of Animal & Plant Sciences 4: 341–356.Google Scholar
Nzawele, DB, Blomme, G, Dheda, DB, Rweyemam, CL, Velly, D, Vigheri, N, Milango, A, Eden-Green, S, Karamura, E and Maerere, AP (2010) The alarming spread of banana Xanthomonas wilt in eastern Democratic Republic of Congo and it impact on food security and income. Tree and Forestery Science and Biotechnology 4: 56–59.Google Scholar
Onyango, M, Haymer, D, Keeley, S and Manshardt, R (2010) Analysis of genetic diversity and relationships in East African ‘Apple Banana’ (AAB genome) and ‘Muraru’ (AA genome) dessert bananas using microsatellite markers. Acta Horticulturae 879: 623–636.Google Scholar
Opara, UL, Jacobson, D and Al-saady, NA (2010) Analysis of genetic diversity in banana cultivars (Musa cvs.) from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses. Journal of Zhejiang Uni 332 v-Sci B (Biomed & Biotechnol) 11: 332–341.CrossRefGoogle ScholarPubMed
Palmer M (2012) Principal component analysis. Available at www.ordination.okstate.edu/PCA.htm.Google Scholar
Pecrot, A (1958) Carte des sols et de la végétation du Congo Belge et du Rwanda Urundi. 16 Dorsale du Kivu. A. sols, carte de reconnaissance. Bruxelles: INEAC.Google Scholar
Pestana, RKN, Amorim, EP, Ferreira, CF, Amorim, VBO, Oliveira, LS, Ledo, CAS and Silva, SO (2011) Genetic dissimilarity of putative gamma-ray-induced ‘Preciosa – AAAB-Pome type’ banana (Musa sp) mutants based on multivariate statistical analysis. Genetic and Molecular Research 10: 3976–3986.Google Scholar
Robinson, JC (1996) Bananas and Plantains. Wallingford: CAB International.Google Scholar
Sebasigari, K (1987) Morphological taxonomy of Musa in Eastern Africa. In: Persley, GJ and De Langhe, EA (eds) Proceedings of an International Workshop on Banana and Plantains Breeding Strategies, 13–17 October 1986, Cairns, Australia, pp. 172–176.Google Scholar
Simmonds, NW and Shepherd, K (1955) The taxonomy and origins of the cultivated bananas. Journal of Linnean Society of London Botany 55: 302–312.CrossRefGoogle Scholar
Sneath, PHA and Sokal, RR (1973) Numerical Taxonomy: the Principles and Practice of Numerical Classification. San Francisco, CA: Freeman WH and Company.Google Scholar
Stover, RH and Simmonds, NW (1991) Bananas. Tropical Agriculture Series, 3rd edn.London: Longman Scientific & Technical.Google Scholar
Swennen, R (1990) Limits of morphotaxonomy: names and synonyms of plantains in Africa and elsewhere. In: Jarret, RL (ed.) Proceeding of Identification of Genetic Diversity in the Genus Musa, 5–10 September 1988, Los Banos, Philippines, pp. 172–176.Google Scholar
Swennen, R and Vuylsteke, D (1987) Morphological taxonomy of plantains (Musa cultivar AAB) in West Africa. In: Persley, G and De Langhe, E (eds) Proceeding of Banana and Plantains Breeding, 13–17 October 1986, Cairns, Australia, pp. 165–171.Google Scholar
Tezenas Du Montcel, H, De Langhe, E and Swennen, R (1983) Essai de Classification des bananiers plantains (AAB). Fruits 38: 461–474.Google Scholar
Ude, G, Pillay, M, Nwakanma, D and Tenkouano, A (2002) Genetic Diversity in Musa acuminata Colla and Musa balbisiana Colla and some of their natural hybrids using AFLP markers. Theoretical and Applied Genetics 104: 1246–1252.Google Scholar
Vuylsteke, D, Swennen, R and De Langhe, E (1991) Somaclonal variation in plantains (Musa spp. AAB group) derived from shoot-tip culture. Fruits 46: 429–439.Google Scholar
Wiley, EO (1981) Phylogenetics: the Theory and Practice of Phylogenetic Systematics. New York/Chichester/Brisbane/Toronto: John Wiley & Sons, Inc, pp. 348–365.Google Scholar
World, CG (2012) Available at http://www.worldclimateguide.co.uk/climateguides/democraticrepublicofthecongo/bukavu.php.Google Scholar
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