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Smart Leginon: Fully Automated Cryo-EM Grid Screening for CryoEM using Leginon and Ptolemy

Published online by Cambridge University Press:  22 July 2022

Huihui Kuang ,
Paul Kim ,
Anchi Cheng ,
Kashyap Maruthi ,
Eugene YD Chua ,
Joshua H. Mendez ,
Hui Wei ,
Edward T. Eng ,
Viacheslav Serbynovskyi  and
Clinton S. Potter
...Show all authors
Huihui Kuang
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States Simons Resource for Automated Molecular Microscopy, New York, NY, United States
Paul Kim
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States Simons Resource for Automated Molecular Microscopy, New York, NY, United States Simons Machine Learning Center, New York, NY, United States
Anchi Cheng
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States Simons Resource for Automated Molecular Microscopy, New York, NY, United States
Kashyap Maruthi
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States Simons Resource for Automated Molecular Microscopy, New York, NY, United States
Eugene YD Chua
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States National Center for CryoEM Access and Training, New York, NY, United States Simons Resource for Automated Molecular Microscopy, New York, NY, United States
Joshua H. Mendez
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States Simons Machine Learning Center, New York, NY, United States
Hui Wei
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States Simons Resource for Automated Molecular Microscopy, New York, NY, United States
Edward T. Eng
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States National Center for CryoEM Access and Training, New York, NY, United States Simons Resource for Automated Molecular Microscopy, New York, NY, United States
Viacheslav Serbynovskyi
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States
Clinton S. Potter
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States National Center for CryoEM Access and Training, New York, NY, United States Simons Resource for Automated Molecular Microscopy, New York, NY, United States Simons Machine Learning Center, New York, NY, United States
Bridget Carragher
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States National Center for CryoEM Access and Training, New York, NY, United States Simons Resource for Automated Molecular Microscopy, New York, NY, United States Simons Machine Learning Center, New York, NY, United States
Alex J. Noble*
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States Simons Resource for Automated Molecular Microscopy, New York, NY, United States Simons Machine Learning Center, New York, NY, United States
Tristan Bepler*
Affiliation:
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, United States Simons Machine Learning Center, New York, NY, United States
*
*Corresponding authors: anoble@nysbc.org; tbepler@nysbc.org
*Corresponding authors: anoble@nysbc.org; tbepler@nysbc.org

Abstract

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Type
Technical Advances in Cryo-EM
Information
Microscopy and Microanalysis , Volume 28 , Supplement S1 , August 2022 , pp. 1266 - 1268
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Copyright
Copyright © Microscopy Society of America 2022

References

Cheng, A., et al. , Leginon: New features and applications. Protein Sci, 30 (2021): p. 136-150. doi: 10.1002/pro.3967CrossRefGoogle ScholarPubMed
Kim, P., et al. , Learning to automate cryo-electron microscopy data collection with Ptolemy. arXiv preprint arXiv:211201534. 2021.Google Scholar
Lander, G.C., et al. , Appion: an integrated, database-driven pipeline to facilitate EM image processing. J Struct Biol, 166 (2009), p. 95-102. doi: 10.1016/j.jsb.2009.01.002CrossRefGoogle ScholarPubMed
Rice, W., et al. Routine determination of ice thickness for cryo-EM grids. Journal of Structural Biology, 204 (2018), p. 38-44. doi: 10.1016/j.jsb.2018.06.007CrossRefGoogle ScholarPubMed