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Elucidating the 3D Structure of β-(1,3)-glucan Synthase from Candida glabrata by Subtomogram Averaging

Published online by Cambridge University Press:  22 July 2022

Jennifer Jiang ,
Cristina Jiménez-Ortigosa ,
Muyuan Chen ,
Kelley R. Healey ,
Joyce Kong ,
Yun-Kyung Lee ,
Daniel P. Farrell ,
Frank DiMaio ,
David S. Perlin  and
Wei Dai
Jennifer Jiang
Affiliation:
Institute for Quantitative Biomedicine, Rutgers, The State University of New Jersey, Piscataway, NJ, United States Department of Cell Biology and Neuroscience, School of Arts and Sciences, Rutgers, The State University of New Jersey, Piscataway, NJ, United States
Cristina Jiménez-Ortigosa
Affiliation:
Hackensack Meridian Health-Center for Discovery and Innovation, Nutley, NJ, United States
Muyuan Chen
Affiliation:
SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA, United States
Kelley R. Healey
Affiliation:
Department of Biology, William Paterson University, Wayne, NJ, United States
Joyce Kong
Affiliation:
Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, United States
Yun-Kyung Lee
Affiliation:
Institute for Quantitative Biomedicine, Rutgers, The State University of New Jersey, Piscataway, NJ, United States Department of Cell Biology and Neuroscience, School of Arts and Sciences, Rutgers, The State University of New Jersey, Piscataway, NJ, United States
Daniel P. Farrell
Affiliation:
Department of Biochemistry, University of Washington, Seattle, WA, United States; Institute for Protein Design, University of Washington, Seattle, WA, United States Institute for Protein Design, University of Washington, Seattle, WA, United States
Frank DiMaio
Affiliation:
Department of Biochemistry, University of Washington, Seattle, WA, United States; Institute for Protein Design, University of Washington, Seattle, WA, United States Institute for Protein Design, University of Washington, Seattle, WA, United States
David S. Perlin
Affiliation:
Hackensack Meridian Health-Center for Discovery and Innovation, Nutley, NJ, United States
Wei Dai*
Affiliation:
Institute for Quantitative Biomedicine, Rutgers, The State University of New Jersey, Piscataway, NJ, United States Department of Cell Biology and Neuroscience, School of Arts and Sciences, Rutgers, The State University of New Jersey, Piscataway, NJ, United States
*
*Corresponding author: wd157@dls.rutgers.edu

Abstract

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Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Information
Microscopy and Microanalysis , Volume 28 , Supplement S1 , August 2022 , pp. 1120 - 1123
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Copyright
Copyright © Microscopy Society of America 2022

References

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Tomographic data collected at Purdue University was supported by the National Institutes of Health Midwest Consortium for High Resolution Cryo-electron Microscopy (U24 GM116789-01A1). This work was supported by the National Institutes of Health (R01AI109025) to D.S.P. The authors acknowledge Jason T. Kaelber and Emre Firlar at the Rutgers CryoEM & Nanoimaging Facility for their support in data collection.Google Scholar