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Effects of QDs@Gd3+-NGR on targeted fluorescence-magnetic resonance imaging and inhibition of pancreatic cancer cells

Published online by Cambridge University Press:  16 March 2020

Qinqin Yan ,
Lin Wang ,
Fengxiang Song ,
Yijun Zhang ,
Lu Gao ,
Fengjun Liu  and
Yuxin Shi
Qinqin Yan
Affiliation:
Department of Radiology, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; and Shanghai Medical Imaging Institute, Fudan University, Shanghai 200032, China
Lin Wang
Affiliation:
Department of Radiology, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Fengxiang Song
Affiliation:
Department of Radiology, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Yijun Zhang
Affiliation:
Department of Radiology, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Lu Gao
Affiliation:
Department of Radiology, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Fengjun Liu*
Affiliation:
Department of Radiology, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Yuxin Shi*
Affiliation:
Department of Radiology, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; and Shanghai Medical Imaging Institute, Fudan University, Shanghai 200032, China
*
a)Address all correspondence to these authors. e-mail: liufengjun121@126.com
b)e-mail: shiyx828288@163.com
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Abstract

Pancreatic cancer is currently one of the most lethal tumors because of delayed diagnosis and treatment. Aminopeptidase N (CD13/APN), expressed in pancreatic cancer cells, is closely related to the malignant biological behavior, for instance, angiogenesis formation, tumor proliferation, and metastasis. In this study, asparagine–glycine–arginine (Asn–Gly–Arg, NGR), selectively binding to CD13 receptor, was modified to construct a novel contrast agent of QDs@Gd3+-NGR for targeted diagnosis and treatment of pancreatic cancer. It consists of QDs-unit for fluorescence imaging, Gd3+-unit for magnetic resonance imaging (MRI), and NGR for binding to CD13 receptor. PANC-1 cells labeled by QDs@Gd3+-NGR showed significant red fluorescence and high intensity on fluorescence and MR imaging, respectively. Besides, it was confirmed that QDs@Gd3+-NGR could inhibit theproliferation, metastasis, and invasion of PANC-1 cells, and increase reactive oxygen species production and death rate in vitro. Reasonably, we believe the targeted contrast agent of QDs@Gd3+-NGR can sensitively detect pancreatic cancer via MR-fluorescence dual-modality imaging, and plays an active role in inhibition of tumor progression. The promising results in this study provide integration of diagnostic and therapeutic strategy for the management of pancreatic cancer in future.

Keywords

nanoprobepancreatic cancermagnetic resonance-fluorescent dual-modality imaginginhibition of tumor cells
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 6 , 30 March 2020 , pp. 591 - 599
Copyright
Copyright © Materials Research Society 2020

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Footnotes

c)

These authors contributed equally to this work.

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