Book contents
- Principles of IVF Laboratory Practice
- Principles of IVF Laboratory Practice
- Copyright page
- Contents
- Contributors
- Foreword
- The Evolution of IVF Practice
- Section 1 Starting a New Laboratory and Training Protocols
- Section 2 Pre-procedure Protocols
- Section 3 Gametes
- Section 4 Insemination/ICSI
- Section 5 Fertilization Assessment
- Section 6 Embryo Assessment: Morphology and Beyond
- Chapter 29 Polar Body Biopsy for IVF
- Chapter 30 Embryo Assessment at the Pre-compaction Stage in the IVF Laboratory
- Chapter 31 Embryo Assessment at the Post-compaction Stage in the IVF Laboratory
- Chapter 32 Embryo Assessment at the Blastocyst Stage in the IVF Laboratory
- Chapter 33 Trophectoderm Biopsy for Pre-implantation Genetic Testing
- Chapter 34 Embryo Culture by Time-Lapse: Selection and Beyond
- Section 7 Embryo Cryopreservation
- Section 8 Embryo Transfer
- Section 9 Quality Management
- Index
- References
Chapter 32 - Embryo Assessment at the Blastocyst Stage in the IVF Laboratory
from Section 6 - Embryo Assessment: Morphology and Beyond
Published online by Cambridge University Press: 07 August 2023
- Principles of IVF Laboratory Practice
- Principles of IVF Laboratory Practice
- Copyright page
- Contents
- Contributors
- Foreword
- The Evolution of IVF Practice
- Section 1 Starting a New Laboratory and Training Protocols
- Section 2 Pre-procedure Protocols
- Section 3 Gametes
- Section 4 Insemination/ICSI
- Section 5 Fertilization Assessment
- Section 6 Embryo Assessment: Morphology and Beyond
- Chapter 29 Polar Body Biopsy for IVF
- Chapter 30 Embryo Assessment at the Pre-compaction Stage in the IVF Laboratory
- Chapter 31 Embryo Assessment at the Post-compaction Stage in the IVF Laboratory
- Chapter 32 Embryo Assessment at the Blastocyst Stage in the IVF Laboratory
- Chapter 33 Trophectoderm Biopsy for Pre-implantation Genetic Testing
- Chapter 34 Embryo Culture by Time-Lapse: Selection and Beyond
- Section 7 Embryo Cryopreservation
- Section 8 Embryo Transfer
- Section 9 Quality Management
- Index
- References
Summary
Embryo quality assessment and grading are major procedures in in vitro fertilization (IVF) laboratories and determining factors for clinical outcome success. Transferring in vitro cultured embryos at the blastocyst stage (generally day 5 to 6 after insemination), including elective single-embryo transfer (eSET), allows better embryo selection and has been associated with higher implantation rates and better clinical outcomes. Blastocyst transfer has become the preferred choice, replacing cleavage-stage (days 2 to 4) embryo transfer, among IVF laboratories worldwide. Recent advancements in embryo culture media, culture conditions and equipment, trophectoderm (TE) biopsy for pre- implantation genetic testing (PGT) and the concept of selecting only a few high-implantation-potential embryos for cryopreservation have contributed to more clinics practicing extended embryo culture to the blastocyst stage. Therefore, blastocyst quality assessment has become an essential and important protocol. This chapter discusses essential steps for blastocyst assessment as a quick guideline and lists blastocyst grading criteria based on three commonly used scoring systems in the reference protocol.
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- Principles of IVF Laboratory PracticeLaboratory Set-Up, Training and Daily Operation, pp. 232 - 239Publisher: Cambridge University PressPrint publication year: 2023