Book contents
- Manual of Sperm Function Testing in Human Assisted Reproduction
- Cambridge Laboratory Manuals in Assisted Reproductive Technology
- Manual of Sperm Function Testing in Human Assisted Reproduction
- Copyright page
- Dedication
- Contents
- Contributors
- Short Biography
- Foreword
- Preface
- Introduction
- Chapter 1 Standard Semen Examination: Manual Semen Analysis
- Chapter 2 Standard Semen Analysis: Computer-Assisted Semen Analysis
- Chapter 3 Standard Semen Analysis: Home Sperm Testing
- Chapter 4 Standard Semen Analysis: Leukocytospermia
- Chapter 5 Standard Semen Analysis: Morphology
- Chapter 6 Sperm Vitality: Eosin-Nigrosin Dye Exclusion
- Chapter 7 Sperm Vitality: Hypo-Osmotic Swelling Test
- Chapter 8 Determination of Mitochondrial Membrane Potential by Flow Cytometry in Human Sperm Cells
- Chapter 9 Capacitation and Acrosome Reaction: Fluorescence Techniques to Determine Acrosome Reaction
- Chapter 10 Capacitation and Acrosome Reaction: Histochemical Techniques to Determine Acrosome Reaction
- Chapter 11 Zona Binding: Competitive Sperm-Binding Assay
- Chapter 12 Zona Binding: Hemizona Assay
- Chapter 13 Oolemma Binding: Sperm Penetration Assay
- Chapter 14 Oxidative Stress Testing: Direct Tests
- Chapter 15 Oxidative Stress Testing: Indirect Tests
- Chapter 16 Chromatin Condensation: Aniline Blue Stain
- Chapter 17 Chromatin Condensation: Chromomycin A3 (CMA3) Stain
- Chapter 18 Sperm Chromatin Structure: Toluidine Blue Staining
- Chapter 19 DNA Damage: TdT-Mediated dUTP Nick-End-Labelling Assay
- Chapter 20 DNA Damage: Sperm Chromatin Structure Assay
- Chapter 21 DNA Damage: COMET Assay
- Chapter 22 DNA Damage: Halo Sperm Test
- Chapter 23 DNA Damage: Fluorescent In-Situ Hybridization
- Chapter 24 Clinical Value of Sperm Function Tests
- Chapter 25 Future Developments: Sperm Proteomics
- Conclusion
- Index
- References
Chapter 19 - DNA Damage: TdT-Mediated dUTP Nick-End-Labelling Assay
Published online by Cambridge University Press: 05 April 2021
- Manual of Sperm Function Testing in Human Assisted Reproduction
- Cambridge Laboratory Manuals in Assisted Reproductive Technology
- Manual of Sperm Function Testing in Human Assisted Reproduction
- Copyright page
- Dedication
- Contents
- Contributors
- Short Biography
- Foreword
- Preface
- Introduction
- Chapter 1 Standard Semen Examination: Manual Semen Analysis
- Chapter 2 Standard Semen Analysis: Computer-Assisted Semen Analysis
- Chapter 3 Standard Semen Analysis: Home Sperm Testing
- Chapter 4 Standard Semen Analysis: Leukocytospermia
- Chapter 5 Standard Semen Analysis: Morphology
- Chapter 6 Sperm Vitality: Eosin-Nigrosin Dye Exclusion
- Chapter 7 Sperm Vitality: Hypo-Osmotic Swelling Test
- Chapter 8 Determination of Mitochondrial Membrane Potential by Flow Cytometry in Human Sperm Cells
- Chapter 9 Capacitation and Acrosome Reaction: Fluorescence Techniques to Determine Acrosome Reaction
- Chapter 10 Capacitation and Acrosome Reaction: Histochemical Techniques to Determine Acrosome Reaction
- Chapter 11 Zona Binding: Competitive Sperm-Binding Assay
- Chapter 12 Zona Binding: Hemizona Assay
- Chapter 13 Oolemma Binding: Sperm Penetration Assay
- Chapter 14 Oxidative Stress Testing: Direct Tests
- Chapter 15 Oxidative Stress Testing: Indirect Tests
- Chapter 16 Chromatin Condensation: Aniline Blue Stain
- Chapter 17 Chromatin Condensation: Chromomycin A3 (CMA3) Stain
- Chapter 18 Sperm Chromatin Structure: Toluidine Blue Staining
- Chapter 19 DNA Damage: TdT-Mediated dUTP Nick-End-Labelling Assay
- Chapter 20 DNA Damage: Sperm Chromatin Structure Assay
- Chapter 21 DNA Damage: COMET Assay
- Chapter 22 DNA Damage: Halo Sperm Test
- Chapter 23 DNA Damage: Fluorescent In-Situ Hybridization
- Chapter 24 Clinical Value of Sperm Function Tests
- Chapter 25 Future Developments: Sperm Proteomics
- Conclusion
- Index
- References
Summary
Male infertility affects men worldwide with about 20 percent of couples having male factor infertility [1]. The routine semen analysis is the first step in the assessment of male infertility. However, conventional semen analysis does not provide a complete understanding of fertility potential, especially in patients with idiopathic infertility [2]. In this scenario, DNA integrity is the most important feature to ensure normal fertilization, implantation, pregnancy and embryonic development. Sperm DNA fragmentation (SDF) can be due to several intrinsic factors such as varicocele, oxidative stress, apoptosis, and chromatin packaging defects. Further, SDF can be caused by extrinsic factors such as lifestyle alterations, infections, exposure to xenobiotics, etc. [3–7].
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- Manual of Sperm Function Testing in Human Assisted Reproduction , pp. 163 - 191Publisher: Cambridge University PressPrint publication year: 2021