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Chapter 16 - Chromatin Condensation: Aniline Blue Stain

Published online by Cambridge University Press:  05 April 2021

Ashok Agarwal
Affiliation:
The Cleveland Clinic Foundation, Cleveland, OH
Ralf Henkel
Affiliation:
University of the Western Cape, South Africa
Ahmad Majzoub
Affiliation:
Hamad Medical Corporation, Doha
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Summary

Spermatozoa, one of the two most pivotal cells of biological existence, are responsible for mediating the transfer of genetic information to subsequent generations. Mammalian fertilization and subsequent embryonic development depend in part on the inherent integrity of sperm genome. Different fertility societies around the globe and the World Health Organization (WHO) estimate that infertility is present in between 7 and 15 percent of couples of reproductive age [1].

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Publisher: Cambridge University Press
Print publication year: 2021

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References

Louis, JF, Thoma, ME, Sørensen, DN, McLain, AC, King, RB, Sundaram, R, Keiding, N, Buck Louis, GM. The prevalence of couple infertility in the United States from a male perspective: evidence from a nationally representative sample. Andrology 2013; 1: 741–8.Google Scholar
Bungum, M. Sperm DNA integrity assessment: a new tool in diagnosis and management of male infertility. Obstet Gynecol Int 2012; 2012: 531042.Google Scholar
Cissen, M, Wely, MV, Scholten, I, Mansell, S, de Bruin, JP, Mol, BW, Bratt, D, Repping, S, Hamer, G. Measuring sperm DNA fragmentation and clinical outcomes of medically assisted reproduction: a systematic review and meta-analysis. PLOS One 2016; 11(11): e0165125.Google Scholar
Aitken, RJ, de Iuliis, GN, McLachlan RI. Biological and clinical significance of DNA damage in the male germ line. Int J Androl 2008; 32: 4656.CrossRefGoogle ScholarPubMed
Barratt, CL, Aitken, RJ, Bjorndahl, L, Carrell, DT, de Boer, P, Kvist, U, Lewis, SE, Perreault, SD, Perry, MJ, Ramos, L, Robaire, B, Ward, S, Zini, A. Sperm DNA: organization, protection and vulnerability: from basic science to clinical applications–a position report. Hum Reprod 2010; 25: 824–38.CrossRefGoogle Scholar
Shamsi, MB, Inam, SN, Dada, R. Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility. J Assist Reprod Genet 2011; 28: 1073–85.CrossRefGoogle ScholarPubMed
Lewis, SE, Agbaje, I, Alvarez, J. Sperm DNA tests as useful adjuncts to semen analysis. Syst Biol Reprod Med 2008; 54(3): 111–25.Google Scholar
Aitken, RJ, De Iuliis, GN. On the possible origins of DNA damage in human spermatozoa. Mol Hum Reprod 2010; 16: 313.Google Scholar
Champroux, A, Torres-Carreira, J, Gharagozloo, P, Drevet, JR, Kocer, A. Mammalian sperm nuclear organization: resiliences and vulnerabilities. Basic Clin Androl 2016; 26: 17.CrossRefGoogle ScholarPubMed
Dhawan, V, Kumar, M, Dada, R. Effect of sperm molecular factors, oxidative damage and transcripts in childhood disorders. J Child Dev Disord 2017; 3(1): 6.CrossRefGoogle Scholar
Dhawan, V, Kumar, M, Deka, D, Malhotra, N, Singh, N, Dadhwal, V, Dada, R. Paternal factors and embryonic development: role in recurrent pregnancy loss. Andrologia 2018; 51(1): e13171.CrossRefGoogle ScholarPubMed
Rathke, C, Baarends, WM, Awe, S, Renkawitz-Pohl, R. Chromatin dynamics during spermiogenesis. Biochem Biophys Acta 2014; 1839: 155–68.Google ScholarPubMed
Dadoune, JP. Expression of mammalian spermatozoal nucleoproteins. Microsc Res Tech 2003; 61: 5675.Google Scholar
Ward, WS. Function of sperm chromatin structural elements in fertilization and development. Mol Hum Reprod 2010; 16: 30–6.CrossRefGoogle ScholarPubMed
Miller, D, Brinkworth, M, Iles, D. Paternal DNA packaging in spermatozoa: more than the sum of its parts? DNA, histones, protamines and epigenetics. Reproduction 2010; 139: 287301.CrossRefGoogle Scholar
Krawetz, SA. Paternal contribution: new insights and future challenges. Nat Rev Genet 2005; 6: 633–42.CrossRefGoogle ScholarPubMed
Jenkins, TG, Carrell, DT. The sperm epigenome and potential implications for the developing embryo. Reproduction 2012; 143(6): 727–34.CrossRefGoogle ScholarPubMed
Kumar, M, Kumar, K, Jain, S, Hassan, T, Dada, R. Novel insights into genetic and epigenetic paternal contribution to the human embryo. Clinics 2013; 68(S1): 514.CrossRefGoogle Scholar
Kumar, M, Dhawan, V, Kranthi, V, Dada, R. Paternal factors: role in idiopathic recurrent pregnancy losses. Int J Reprod Fertil Sex Health S2; 001: 16.Google Scholar
Hammoud, SS, Nix, DA, Zhang, H, Purwar, J, Carrell, DT, Cairns, BR. Distinctive chromatin in human sperm packages genes for embryo development. Nature 2009; 460(7254): 473–8.CrossRefGoogle ScholarPubMed
Carrell, DT, Emery, BR, Hammoud, S. Altered protamine expression and diminished spermatogenesis: what is the link? Hum Reprod Update 2007; 13: 313–27.Google Scholar
Wykes, SM, Krawetz, SA. The structural organization of sperm chromatin. J Biol Chem 2003; 278: 29471–7.Google Scholar
Carrell, DT, Hammoud, S. The human sperm epigenome and its potential role in embryonic development. Mol Hum Reprod 2010; 16(1): 3747.Google Scholar
Dada, R. Sperm DNA damage diagnostics: when and why? Transl Androl Urol 2017; 6(Suppl. 4): S691–4Google Scholar
Sellami, A, Chakroun, N, Zarrouk, BS, Sellami, H, Kebaili, S, Rebai, T, Keskes, L. Assessment of chromatin maturity in human spermatozoa: useful aniline blue assay for routine diagnosis of male infertility. Adv Urol 2013; 2013: 1–8.CrossRefGoogle Scholar
Sakkas, D, Mariethoz, E, St. John, JC. Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the Fas-mediated pathway. Exp Cell Res 1999; 251: 350–5.Google Scholar
Aitken, RJ, De Iuliis, GN. Origins and consequences of DNA damage in male germ cells. Reprod Biomed Online 2007; 14: 727–33.Google Scholar
Hammadeh, M, Zeginiadov, T, Rosenbaum, P. Predictive value of sperm chromatin condensation (aniline blue staining) in the assessment of male fertility. Arch Androl 2001; 46: 99104.Google Scholar
Agarwal, A, Said, MT. (2004) Sperm Chromatin Assessment. Abingdon: Taylor and Francis, pp. 93103.Google Scholar
Aoki, VW, Moskovtsev, SI, Willis, J, Liu, L, Mullen, JB, Carrell, DT. DNA integrity is compromised in protamine-deficient human sperm. J Androl 2005; 26: 741–8.Google Scholar
Hammadeh, ME, Hasani, SA, Stieber, M, Rosenbaum, P, Kupker, D, Diedrich, K, Schmidt, W. The effect of chromatin condensation (Aniline Blue staining) and morphology (strict criteria) of human spermatozoa on fertilization, cleavage and pregnancy rates in an intracytoplasmic sperm injection programme. Hum Reprod 1996; 11: 2468–71.CrossRefGoogle Scholar
Erenpreiss, J, Bars, J, Lipatnikova, V, et al. Comparative study of cytochemical tests for sperm chromatin integrity. J Androl 2001; 22: 4553.CrossRefGoogle ScholarPubMed
Agarwal, A, Majzoub, A, Esteves, SC, Ko, E, Ramasamy, R, Zini, A. Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios. Trans Androl Urol 2016; 5(6): 935–50.CrossRefGoogle ScholarPubMed
Muratori, M, Tamburrino, L, Marchiani, S, Cambi, M, Olivito, B, Azzari, C, et al. Investigation on the origin of sperm DNA fragmentation: role of apoptosis, immaturity and oxidative stress. Mol Med 2015; 21: 109–22.Google Scholar
Pourmasumi, S, Khoradmehr, A, Rahiminia, T, Sabeti, P, Talebi, AR, Ghasemzadeh, J. Evaluation of sperm chromatin integrity using Aniline Blue and Toluidine Blue staining in infertile and normozoospermic men. J Reprod Infertil 2019; 20(2): 90101.Google Scholar
Talebi, AR, Sarcheshmeh, AA, Khalili, MA, Tabibnejad, N. Effects of ethanol consumption on chromatin condensation and DNA integrity of epididymal spermatozoa in rat. Alcohol 2011; 45(4): 403–9.CrossRefGoogle ScholarPubMed
Irez, T, Dayioglu, N, Alagoz, M, Karatas, , Guralp, O. The use of aniline blue chromatin condensation test on prediction of pregnancy in mild male factor and unexplained male infertility. Andrologia 2018; 50(10): e13111.Google Scholar
Ahmad, SA, Umar, LA, Lestari, SW, Mansyur, E, Hestiantoro, A, Paradowszka-Dogan, A. Sperm chromatin maturity and integrity correlated to zygote development in ICSI program. Syst Biol Reprod Med. 2016; 62(5): 309–16.Google Scholar
Simon, L, Brunborg, G, Stevenson, M, Lutton D, McManus J, Lewis, SE. Clinical significance of sperm DNA damage in assisted reproduction outcome. Hum Reprod 2010; 25:1594–608.CrossRefGoogle ScholarPubMed
Zini, A. Are sperm chromatin and DNA defects relevant in the clinic? Syst Biol Reprod Med 2011; 57: 7885.Google Scholar
Lin, MH, Lee, RK, Li, SH,Lu, CH, Sun, FJ, Hwu, YM. Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates. Fertil Steril 2008; 90(2): 352–9.CrossRefGoogle Scholar
Esterhuizen, AD, Franken, DR, Lourens, JGH, van Zyl, C, Müller I, , Van Rooyen, LH. Chromatin packaging as an indicator of human sperm dysfunction. J Assist Reprod Genet 2000; 17(9): 508–14.Google Scholar
Sadeghi, MR, Hodjat, M, Lakpour, N et al. Effects of sperm chromatin integrity on fertilization rate and embryo quality following intracytoplasmic sperm injection. Avicenna J Med Biotechnol 2009; 1(3): 173–80.Google ScholarPubMed
Razavi, S, Nasr-Esfahani, MH, Mardani, M, Mafi, A, Moghdam, A. Effect of human sperm chromatin anomalies on fertilization outcome post-ICSI. Andrologia 2003; 35(4): 238–43.CrossRefGoogle ScholarPubMed
Abu, DA, Franken, DR, Hoffman, B, Henkel, R. Sequential analysis of sperm functional aspects involved in fertilization: a pilot study. Andrologia 2012; 44(Suppl. 1): 175–81.Google Scholar
Jayaraman, V, Upadhya, D, Narayan, PK, Adiga, SK. Sperm processing by swim‐up and density gradient is effective in elimination of sperm with DNA damage. J Assist Reprod Genet 2012; 29(6): 557–63.Google Scholar
Kazerooni, T, Asadi, N, Jadid, L, Kazerooni, M, Ghanadi, A, Ghaffarpasand, F, et al. Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion. J Assist Reprod Genet 2009; 26(11–12): 591–6.CrossRefGoogle ScholarPubMed
Palermo, GD, Neri, QV, Cozzubbo, T, Rosenwaks, Z. Perspectives on the assessment of human sperm chromatin integrity. Fertil Steril 2014; 102: 1508–17.Google Scholar

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