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Chapter 9 - Capacitation and Acrosome Reaction: Fluorescence Techniques to Determine Acrosome Reaction

Published online by Cambridge University Press:  05 April 2021

Ashok Agarwal
The Cleveland Clinic Foundation, Cleveland, OH
Ralf Henkel
University of the Western Cape, South Africa
Ahmad Majzoub
Hamad Medical Corporation, Doha
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The acrosome reaction (AR) is an essential process of spermatozoa for fertilization and is characterized by the exocytosis of the acrosomal content and the release of hybrid membrane vesicles formed by patches of the outer acrosomal membrane and the plasma membrane. Through acomplex membrane fusion process, which exposes the inner acrosomal membrane, both the morphology of the plasma membrane and the distribution of proteins involved in sperm–egg interaction are changed [1, 2]. AR also modifies sperm function, since acrosome-reacted sperm are no longer able to bind to the zona pellucida, but to the oolemma [3].

Publisher: Cambridge University Press
Print publication year: 2021

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