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To verify the effectiveness and safety of the addition of adipose-derived regenerative cells to autologous fat injection therapy.
Unilateral vocal fold paralysis models were made by cutting the right recurrent laryngeal nerve in two pigs. At day 30, 0.5 ml adipose-derived regenerative cells mixed with 1 ml autologous fat was injected into the right vocal fold of one pig, with the other receiving 0.5 ml Ringer's solution mixed with 1 ml autologous fat. At day 120, fibrescopy, laser Doppler flowmeter, computed tomography, vocal function evaluation and histological assessment were conducted.
Although histological assessment revealed atrophy of the thyroarytenoid muscle fibre in both pigs, there was remarkable hypertrophy of the thyroarytenoid muscle fibre in the area surrounding the adipose-derived regenerative cells injection site.
The addition of a high concentration of adipose-derived regenerative cells to autologous fat injection therapy has the potential to improve the treatment outcome for unilateral vocal fold paralysis.
The aim of this study was to estimate genetic correlations between milk yield, somatic cell score (SCS), mastitis, and claw and leg disorders (CLDs) during first lactation in Holstein cows by using a threshold–linear random regression test-day model. We used daily records of milk, fat and protein yields; somatic cell count (SCC); and mastitis and CLD incidences from 46 771 first-lactation Holstein cows in Hokkaido, Japan, that calved between 2000 and 2009. A threshold animal model for binary records (mastitis and CLDs) and linear animal model for yield traits were applied in our multiple trait analysis. For both liabilities and yield traits, additive genetic effects were used as random regression on cubic Legendre polynomials of days on milk. The highest positive genetic correlations between yields and disease incidences (0.36 for milk and mastitis, 0.56 for fat and mastitis, 0.24 for protein and mastitis, 0.32 for milk and CLD, 0.44 for fat and CLD and 0.31 for protein and CLD) were estimated at about the time of peak milk yield (36 to 65 days in milk). Selection focused on early lactation yield may therefore increase the risk of mastitis and CLDs. The positive genetic correlations of SCS with mastitis or CLD incidence imply that selection to reduce SCS in the early stages of lactation would decrease the incidence of both mastitis and CLD.
To increase X-ray photon number generated by laser-cluster interaction, it is important to understand the dependence of X-ray generation on cluster size. We carried out Xe K-shell X-ray generation using a conical nozzle with Xe clusters, the radius of which was controllable by adjusting the backing pressure. The experiment clarifies the result that the Xe K-shell X-ray photon number increases with increasing cluster radius from 8 to 12 nm, and saturates at the radius between 12 and 17 nm. We also investigated the Xe K-shell X-ray photon number dependence on laser intensity, and found that the threshold laser intensity of the Xe K-shell X-ray generation exists between 2 × 1017 and 5 × 1018 W/cm2.
Using a newly developed rapid test, an outbreak of human metapneumovirus (HMPV) infection in a long-term care facility was detected within only 2 days after the onset of symptoms in a putative index case. The outbreak was almost under control within 8 days mainly by zoning patients, with the exception of two cases of HMPV that were diagnosed 16 and 17 days after the onset of the outbreak. According to an immunological diagnosis as well as the rapid test, it was eventually proven that 18 patients had HMPV infections. We suspected that even asymptomatic residents, who had not been completely separated from the facility population, were a source of infection. That suggested that all asymptomatic residents should be tested and that the separation of the infected patients should be absolute, if an outbreak of HMPV infection is suspected in such a facility.
We investigated the relationships between conception rates (CRs) at first service in Japanese Holstein heifers (i.e. animals that had not yet had their first calf) and cows and their test-day (TD) milk yields. Data included records of artificial insemination (AI) for heifers and cows that had calved for the first time between 2000 and 2008 and their TD milk yields at 6 through 305 days in milk (DIM) from first through third lactations. CR was defined as a binary trait for which first AI was a failure or success. A threshold-linear animal model was applied to estimate genetic correlations between CRs of heifers or cows and TD milk yield at various lactation stages. Two-trait genetic analyses were performed for every combination of CR and TD milk yield by using the Bayesian method with Gibbs sampling. The posterior means of the heritabilities of CR were 0.031 for heifers, 0.034 for first-lactation cows and 0.028 for second-lactation cows. Heritabilities for TD milk yield increased from 0.324 to 0.433 with increasing DIM but decreased slightly after 210 DIM during first lactation. These heritabilities from the second and third lactations were higher during late stages of lactation than during early stages. Posterior means of the genetic correlations between heifer CR and all TD yields were positive (range, 0.082 to 0.287), but those between CR of cows and milk yields during first or second lactation were negative (range, −0.121 to −0.250). Therefore, during every stage of lactation, selection in the direction of increasing milk yield may reduce CR in cows. The genetic relationships between CR and lactation curve shape were quite weak, because the genetic correlations between CR and TD milk yield were constant during the lactation period.
A patient (64-year-old, male) with familial cholinesterasemia caused
BChE deficiency was
studied. DNA sequence analysis of all exons identified a point mutation,
an A→G transition at codon
128, resulting in a Tyr→Cys substitution. The propositus showed extremely
low BChE activity, but
his other family members (three individuals) showed from intermediate to
normal BChE activity. An
immunological method revealed the absence of BChE protein in serum of the
propositus. Both PCR
primer introduced restriction analysis (PCR-PIRA) and sequence analysis
revealed all three family members to be heterozygotes for this mutation.
Nano-crystalline GaN (nc-GaN) and hydrogenated nc-GaN (nc-GaN:H) thin films and thin film transistors (TFT) prepared by a reactive sputtering method have been studied. Hydrogen incorporation in nc-GaN film induces localized states at mid-gap energy. Thermal annealing at 400 °C and 600 °C creates mid-gap states which is detectable by electron spin resonance. Further thermal annealing treatment at 800 °C reduces the deep states in nc-GaN and nc-GaN:H. Photoluminescence spectra of the nc-GaN film have two broad peaks at 2.4 eV and 3.2 eV. The source-drain current voltage characteristics of the nc-GaN TFT is demonstrated for the first time. The obtained field effect mobility is 10−4 cm2/V-s. Thermal annealing at 800 °C improves the field effect mobility to 10−2 cm2/V-s.
Microstructures of two MPMG processed YBaCuO materials with and without Y2BaCuO5 (211) inclusions were investigated by transmission electron microscopy. Using the MPMG process, it is possible to change the quantity of the 211 inclusions in the YBa2Cu3O7 (123) matrix. We prepared two YBaCuO samples with 0 and 30 vol. % 211 and with respective critical current density values of 2000 and 30 000 A/cm2 at 77 K and 1 T (magnetic field parallel to the c-axis). As possible pinning centers, we found stacking faults in the 123 matrix. However, we observed no appreciable change in their number and structure by introducing the 211 inclusions. Therefore, the difference in Jc values can be attributed to the 211 inclusion itself.
Extremely high-sensitive image pickup tubes with sensitivities 1000 times higher than those of conventional tubes are fabricated using the avalanche phenomenon in a-Se as photoconductive targets. The excess avalanche noise of a video signal is found to be much less than that expected, based on the carrier ionization rates. The frequency spectra of the noise currents of both the pickup tubes and sandwich-type photocells are examined. The results are compared with those of a simulation, and it is found that the excess noise can be reduced by the charge-storage operation of imaging devices.
High quality ZnSe films were successfully grown on GaAs(100) at low temperatures, 200 °C or lower by Hydrogen Radical-enhanced Chemical Vapor Deposition (HRCVD). Defects were makedly eliminated by the following factors: selection of source materials; avoidance of ion bombardment; and suppression of formation of adducts by alternate gas supply. Strained-layer superlattice (SLS) consisting of ZnSe as the well and ZnS0. 1Se0.9 as the barrier was made by this technique. Emission line attributed to the free exciton was dominantly observed in the SLS.
Textured TCO (Sn02:F) substrates were fabricated using atmospheric pressure CVD (APCVD). Various kinds of TCO developed were classified into three types (A, B, C) in terms of shape of texture, electrical and optical properties. TCO substrates of type A with low carrier concentration and pyramidal texture fulfill the conflicting properties of low absorption and low resistivity after hydrogen plasma treatment. By using type A TCO substrates, the highest conversion efficiencies of 12 % for a small area of 1cm×1cm as a-Si single junction solar cell were certified by several related NEDO research organizations.
Giaever tunneling measurements have been applied to investigate the electronic structures of the high Tc superconductor YB2Cu3Oy near Tc. The superconducting energy gap of 17 meV was observed in V-dl/dv curve at 77 K. In the light of BCS model, 2Δ0Δ/kBTc was estimated as 3.3. Several dip structures were observed in the bias region up to 0.5 eV in V-dl/dV curve, using the 3rd derivative analysis. Through the temperature dependence, these structures were found to be associated with the superconductivity.
Since the discovery of high Tc superconducting oxide such as Ln-Ba-Cu-O system which shows superconductivity above liquid nitrogen temperature, intensive study has been carried out to investigate its intrinsic properties[l-4]. Superconducting wire which operates in liquid nitrogen is one of the emerging products among application, however, there are many factors to be investigated for practical use. Critical current density, Jc, is one of the most important characteristics of superconductor, although, only few report describing Jc measurement ever published. In this paper, fabrication procedure of silver sheathed Y-Ba-Cu-O wire by powder metallurgical technique and its superconducting properties have been reported. Other factors which may influence superconductivity such as microstructure of the oxide, configuration of wires, etc. are also discussed.
Tunneling measurements were applied to investigate the density of states of the high Tc superconductor in the temperature range from 4.2 K to the transition temperature. Several structures were found in conductance curves in higher bias region than ever reported, and they vanished around Tc. Temperature dependence of the bias voltages at which these structures appeared showed the BCS-like behavior.
The effect of thermal processing in the sample preparation of YBa2Cu3Oy has been investigated systematically on microstructures and electrical properties. The grain size was found to grow preferably during the cooling process rather than during the sintering process. The high Tc superconducting phase appears around 500 C in this thermal processing. The annealing experiment shows that the grains still grow at low temperature such as 300 °C.
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