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Autologous fat injection therapy including a high concentration of adipose-derived regenerative cells in a vocal fold paralysis model: animal pilot study

Published online by Cambridge University Press:  08 September 2016

N Nishio ,
Y Fujimoto ,
K Suga ,
Y Iwata ,
K Toriyama ,
K Takanari ,
Y Kamei ,
T Yamamoto  and
M Gotoh
N Nishio*
Affiliation:
Department of Otorhinolaryngology, Nagoya University Graduate School of Medicine, Aichi, Japan
Y Fujimoto
Affiliation:
Department of Otorhinolaryngology, Nagoya University Graduate School of Medicine, Aichi, Japan
K Suga
Affiliation:
Department of Otorhinolaryngology, Nagoya University Graduate School of Medicine, Aichi, Japan
Y Iwata
Affiliation:
Department of Otorhinolaryngology, Fujita Health University, Aichi, Japan
K Toriyama
Affiliation:
Department of Plastic and Reconstructive Surgery, Nagoya University Graduate School of Medicine, Aichi, Japan
K Takanari
Affiliation:
Department of Plastic and Reconstructive Surgery, Nagoya University Graduate School of Medicine, Aichi, Japan
Y Kamei
Affiliation:
Department of Plastic and Reconstructive Surgery, Nagoya University Graduate School of Medicine, Aichi, Japan
T Yamamoto
Affiliation:
Department of Urology, Nagoya University Graduate School of Medicine, Aichi, Japan
M Gotoh
Affiliation:
Department of Urology, Nagoya University Graduate School of Medicine, Aichi, Japan
*
Address for correspondence: Dr Naoki Nishio, Department of Otorhinolaryngology, Nagoya University Graduate School of Medicine, 65, Tsurumai-cho, Showa-ku, Nagoya 466–8550, Japan Fax: +11 81 52 744 2325 E-mail: naokin@med.nagoya-u.ac.jp
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Abstract

Objectives:

To verify the effectiveness and safety of the addition of adipose-derived regenerative cells to autologous fat injection therapy.

Methods:

Unilateral vocal fold paralysis models were made by cutting the right recurrent laryngeal nerve in two pigs. At day 30, 0.5 ml adipose-derived regenerative cells mixed with 1 ml autologous fat was injected into the right vocal fold of one pig, with the other receiving 0.5 ml Ringer's solution mixed with 1 ml autologous fat. At day 120, fibrescopy, laser Doppler flowmeter, computed tomography, vocal function evaluation and histological assessment were conducted.

Results:

Although histological assessment revealed atrophy of the thyroarytenoid muscle fibre in both pigs, there was remarkable hypertrophy of the thyroarytenoid muscle fibre in the area surrounding the adipose-derived regenerative cells injection site.

Conclusion:

The addition of a high concentration of adipose-derived regenerative cells to autologous fat injection therapy has the potential to improve the treatment outcome for unilateral vocal fold paralysis.

Keywords

Stem CellsInjectionsVocal Cord ParalysisAnimalHoarseness
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 130 , Issue 10 , October 2016 , pp. 914 - 922
Copyright
Copyright © JLO (1984) Limited 2016 

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