Quizalofop-resistant rice allows for over-the-top applications of quizalofop, a herbicide that inhibits acetyl-coenzyme A carboxylase. However, previous reports have indicated that quizalofop applied postemergence may cause significant injury to quizalofop-resistant rice. Therefore, field experiments were conducted to evaluate the response of quizalofop-resistant rice cultivars to quizalofop applications across different planting dates. Under controlled conditions, the effects of soil moisture content, air temperature, and light intensity on quizalofop-resistant rice sensitivity to quizalofop were investigated. In the planting date experiment, injury of more than 11 percentage points was observed on early-planted rice compared with late-planted rice at the 5-leaf stage, with higher injury observed under saturated soil conditions. However, quizalofop applications at the labeled rate caused ≤16% reduction in yield regardless of planting environment. Quizalofop-resistant cultivars exhibited more injury by at least 25 percentage points when soil was maintained at 90% or 100% of field capacity because rice cultivars ‘PVL01’, ‘PVL02’, and ‘RTv7231 MA’ exhibited ≥42%, 30%, and ≥54% injury, respectively, compared with ≤10%, ≤5%, and ≤22% injury, respectively, at 40% or 50% of field capacity, pooled over rating timing. Greater injury ranging from 18% to 31% was observed on quizalofop-resistant rice grown under low light intensity (600 µmol m−2s−1) compared with 5% to 14% injury under high light intensity (1,150 µmol m−2s−1). The injury persisted from 7 to 28 d after 5-leaf stage application (DAFT), averaged over quizalofop-resistant cultivars and air temperatures (20/15 C and 30/25 C day/night, respectively). At 7 DAFT, greater injury (by 5 to 21 percentage points) was observed on quizalofop-resistant cultivars; PVL01, PVL02, and RTv7231 MA exhibited 33%, 9%, and 58% injury, respectively, under 20/15 C temperature conditions compared with 13%, 4%, and 37% injury, respectively, under 30/25 C day/night conditions averaged over light intensities. Overall, quizalofop is likely to cause a greater risk for injury to quizalofop-resistant rice if it is applied under cool, cloudy, and moist soil conditions.