Two microsatellite markers OarHH35 and BMS2508, closely associated with high levels of reproduction in sheep, were analysed for polymorphisms in Xiangdong black goat, Nanjiang brown goat, Guizhou black goat and Boer goat. The number of alleles for OarHH35 was 11, 8, 7 and 10, respectively, in the four goat breeds. That for BMS2508 was 9, 5, 6 and 7. The polymorphic information content (PIC) values for OarHH35/BMS2508 in the above-mentioned goats were respectively 0.8294/0.8047, 0.8399/0.6894, 0.7985/0.6910 and 0.8582/0.7688. By analysing the dominant effect of the two microsatellite markers on litter size of Xiangdong black goat, it was shown that microsatellite markers BMS2508 and OarHH35 significantly influenced (P<0.05) litter size of goats. At the OarHH35 locus, the least squares means (LSM) of genotype 135/135 bp was the highest (3.67 number born/litter). Moreover, LSM of genotypes 123/135 bp and 125/125 bp also reached 3.00 kids born/litter, indicating that alleles 135 bp and 125 bp exerted a significant positive effect on the litter size in Xiangdong black goat. At the BMS2508 locus, LSM of genotypes 132/145 bp and 93/132 bp for litter size was 4.00 and 3.25 kids born/litter respectively, but that of genotype 122/122 bp for litter size was only 1.00 kid/litter, so alleles 145 bp and 93 bp had significant positive effects on the litter size of Xiangdong black goat, and the allele 122 bp had a significantly negative effect.