Field studies were conducted in 2007 and 2008 at seven sites in Ohio, Indiana, and Illinois to determine the effect of PRE herbicide and POST application timing on weed control and yield of glyphosate-resistant corn. Levels of PRE herbicide included none; low—atrazine; medium—atrazine and metolachlor; and high—atrazine, mesotrione, and metolachlor. Glyphosate was applied POST when corn was 30 cm tall, or 1 or 2 wk later. Common lambsquarters, giant foxtail, and giant ragweed infested at least six of the seven sites, and other weed species occurred at two to three sites. Control of weeds at the time of POST application ranged from 48 to 91%, 58 to 99%, and 87 to 100% for the low, medium, and high levels of PRE herbicide, respectively, averaged over POST application timing. Control of giant foxtail and redroot pigweed decreased by about 20% between the second and third POST timing, averaged over PRE herbicide, but control of other weeds was similar among timings. Late-season control of common ragweed, velvetleaf, common lambsquarters, and Pennsylvania smartweed exceeded 90%, regardless of PRE herbicide or POST timing. Control of redroot pigweed, ivyleaf morningglory, and giant ragweed was as low as 74, 67, and 83%, respectively, but the high level of PRE herbicide resulted in 90 to 97% control of these weeds. An interaction between PRE herbicide and POST timing for late-season control of giant foxtail, tall waterhemp, and yellow nutsedge reflected the more effective control among POST timings from the higher levels of PRE herbicide. The overall trend in this study was for more effective weed control in PRE/POST herbicide programs with more comprehensive PRE herbicides that have substantial activity on both grass and broadleaf weeds. Highest yield occurred where the PRE treatment consisted of a two- or three-way combination of herbicides applied at 50% of the recommended rate or higher. Yield was reduced at all POST timings with atrazine alone or in the absence of PRE herbicide.