The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric
analysis. Male human leukocytes were used as internal reference cells.
Nucleated fish erythrocytes were stained simultaneously to human leukocytes
with a staining buffer containing propidium iodide (PI). Nuclear DNA
contents of target species were estimated in relation to an assigned value
of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (±CI)
was 1.55 ± 0.02 pg for D. labrax, 1.90 ± 0.03 pg for S. aurata, 1.57 ± 0.02 pg for L. ramada, and 2.43 ± 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant differences in genome size between
males and females in either D. labrax or L. ramada.
/ Anguilla anguilla