A fluorometric-based method for the kinetic measurement
of conidia growth of Botrytis cinerea in liquid
culture is described. The
method involves an oxido-reduction dye, Alamar blue, as an
indicator of growth. The effect of nystatin, a non-specific
antifungal agent, was studied on conidial germination. An
excellent correlation was found between the IC50
obtained by the fluorometric
method, and by microscopic measurement. The method is rapid
and quantitative, and requires only a small amount of
chemicals and fungal material. Since the measurements were
performed in a 96-well plate, it is possible to test many
different parameters in parallel.