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Kinetic evaluation of conidial germination of Botrytis cinerea by a spectrofluorometric method

Published online by Cambridge University Press:  01 March 1998

A. L. PELLOUX-PRAYER ,
B. PRIEM  and
J. P. JOSELEAU
A. L. PELLOUX-PRAYER
Affiliation:
Centre de Recherches sur les Macromolécules Végétales, CNRS, affilié à l‘Université Joseph Fourier, BP 53, 38041 Grenoble cedex 9, France
B. PRIEM
Affiliation:
Centre de Recherches sur les Macromolécules Végétales, CNRS, affilié à l‘Université Joseph Fourier, BP 53, 38041 Grenoble cedex 9, France
J. P. JOSELEAU
Affiliation:
Centre de Recherches sur les Macromolécules Végétales, CNRS, affilié à l‘Université Joseph Fourier, BP 53, 38041 Grenoble cedex 9, France
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Abstract

A fluorometric-based method for the kinetic measurement of conidia growth of Botrytis cinerea in liquid culture is described. The method involves an oxido-reduction dye, Alamar blue, as an indicator of growth. The effect of nystatin, a non-specific antifungal agent, was studied on conidial germination. An excellent correlation was found between the IC50 obtained by the fluorometric method, and by microscopic measurement. The method is rapid and quantitative, and requires only a small amount of chemicals and fungal material. Since the measurements were performed in a 96-well plate, it is possible to test many different parameters in parallel.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 3 , March 1998 , pp. 320 - 322
Copyright
The British Mycological Society 1998

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